Objective To investigate the association between Cardiomyopathy associated 5 (CMYA5) polymor?phisms and schizophrenia in the Uygur Chinese population. Methods Taq-man assay was used to detect CMYA5 gene rs3828611 in 684 schizophrenia patients and 678 healthy controls from Chinese population. The positive and negative symptoms scale (PANSS) was used to evaluate patients’symptoms. Results Neither the genotype nor the allele frequen?cies of rs3828611 was significantly different between the patients and the controls (P>0.05). The differences were not sig?nificant in either each gender subgroup or in each age (teenager and adult) subgroup (P>0.05). The total score and the sub scores of PANSS were not significantly different among patients with different genotype groups (P>0.05). Conclu?sions There is no association between CMYA5 rs3828611and schizophrenia in the Uygur Chinese population.

AIM:To study the effects of FK506 binding protein 12.6(FKBP12.6) overexpression on the performance of sarcoplasmic reticulum(SR) in ventricular myocytes of rats with heart failure.METHODS:The adenovirus(Ad)-mediated gene transfer was used to overexpress FKBP12.6 in ventricular myocytes of rats with heart failure.Western blotting and reverse transcriptase-polymerase chain reaction(RT-PCR) analysis were used to reveale specific overexpression of FKBP12.6.X-rhod-1-AM was used as the Ca2+ indicator,and cells were viewed under a confocal microscope.RESULTS:Adenovirus mediated overexpression of FKBP12.6 resulted in a 5-fold increase in relative FKBP12.6 mRNA levels and a 4-fold increase in relative FKBP12.6 protein levels at 48 h after transfection compared with control.The amplitude of the fluorescence [Ca2+]i transient was significantly increased in Ad-FKBP12.6-GFP cardiomyocytes compared with Ad-GFP myocytes(peak F/F0,3.16?0.42 vs 1.43?0.38,P

Objective To evaluate the left ventricular function and the synchrony of myocardial ischemic segments in patients with coronary heart disease (CHD) after PTCA and stent implantation by quantitative tissue velocity imaging (QTVI). Methods Thirty-six patients with isolated left anterior descending stenosis (≥75%) were examined by QTVI three days before, one week and one month after successful PTCA and stent implantation to measure the following items of 5 different left ventricular segments: peak systolic velocity( Vs), early diastolic velocity (Ve), late diastolic velocity (Va), time to peak systolic velocity(Ts). Then the coefficient of variation (SD/mean) of the 5 different Ts were calculated.Results The value of Vs,Ve and Va were decreased and the Ve/Va ratio was reverses three days before PTCA + stent. Compared with that before PTCA + stent,the value of Vs and Ve were increased significantly in one week ( P ＜0. 05) and one month( P ＜0.01 ) after PTCA + stent,respectively,the value of Va was not statistically significant. Ve/Va ratio was recovered in one week after PTCA treatment. Ts and Ts-SD were shorted dramatically in one week( P ＜0. 05) and one month( P ＜0.01 ) after PTCA + stent compared with that before PTCA + stent in which Ts were prolonged more than 33 ms. Conclusions QTVI can quantitatively assess the left ventricular function and the synchrony of myocardial ischemic segments, and can be used to real-time detect the changes of function and synchrony of left ventricle after PTCA and stent implantation.

Objective To evaluate the relationship between cardiomyopathy-associated 5 (CMYAS) gene rs10043986 polymorphism and schizophrenia in Uygur Chinese population.Methods The SNP rs10043986 in CMYA5 gene was genotyped in 325 patients with schizophrenia and 183 normal controls using TaqMan technology.The symptoms of schizophrenia were assessed by positive and negative syndrome scale (PANSS).The association of the loci with schizophrenia,age of onset,clinical symptom was analyzed.Results The allelic and genotypic distributions in rs10043986 between patients with schizophrenia (C,T allele:91.5％,8.5％ ; C/C,C/T,T/T genotypes:83.4％,16.3％,0.3％) and normal controls (C,T allele:96.4％,3.6％ ; C/C,C/T,T/T genotypes:92.9％,7.1％,0) had statistically significance after analysis (x2 =9.038,P=0.003 ; x2 =9.417,P=0.009).Via analysis of stratification by gender and age at onset.The results showed that both allele (x2=11.812,P=0.001) and genotype (x2=12.769,P=0.001) frequency in rs10043986 with patients were significantly different in females,but neither in males (all P＞0.05).Allelic or genotypic distributions between adult cases and controls had statistically significance (x2=8.219,P=0.004; x2=8.379,P=0.015),but there were not significant differences between adolescent cases and controls (all P＞ 0.05).Furthermore,we also notice that the PANSS scores of patients between Genotype C/C and C/T had no statistically significance (allP＞0.05).Conclusion The results reveal that T allele at CMYA5 rs10043986 may be confer risk for susceptibility of female and adult schizophrenia in Uygur Chinese population,and that rs 10043986 polymorphism may not significantly associate with symptoms severity of schizophrenia.

Objective To evaluate the relationship between the C270T polymorphism brain-derived neurotrophic factor and susceptibility of schizophrenia using meta-analysis. Methods A retrieval was performed on the case control study on the C270T polymorphism of the patients with schizophrenia. The meta-analysis was applied for investigating and summarizing the relationship between C270T polymorphism and schizophrenia. Subgroups were divided according to races. Results A total of 16 studies with 3874 patients and 4309 controls were included. The frequencies of C/T allele and genotype CC/(CT+TT) were associated with schizophrenia (all P<0.01) with OR 1.65 [95%CI (1.26, 2.16)] and 1.71 [95%CI (1.27, 2.30)], respectively. The association of C/T allele and genotype CC/(CT+TT) with schizophrenia was signif-icant in Asian subgroup (P<0.01), with OR 1.89 [95%CI (1.30, 2.75)] and 1.97 [95%CI (1.29, 3.03)], but not in Cauca-sian subgroup (all P=0.05). Conclusion The C270T polymorphism of brain-derived neurotrophic factor gene might con-tribute to the genetic susceptibility of schizophrenia in Asian population.

Objective To explore the clinical features, inducements and interventions in a case of mass hysteria. Methods A mass hysteria strook after a fight gang in a factory. Fifty-four cases were diagnosed as mass hysteria. The general information and clinical symptoms of all patients with mass hysteria were collected. All patients were assessed us-ing Hamilton anxiety scale (HAMA) before treatment, and at the end of one, two and four weeks of the treatment, respec-tively. Results The patients were thirty-eight females (70.4%) and sixteen males (29.6%), with average age (20.26±2.04) years old. Fifty patients showed anxious about self-safety mostly. The most common symptoms were convulsions or con-vulsive seizure in forty-seven cases (87.0%), outburst of emotion in thirty-two cases (59.3%), and episodic syncope in twenty-nine cases (53.7%). Thirty-four cases (62.96%) were disscoiative disorders mixed with conversion disorders. The scores of HAMA at the end of one, two and four weeks of the treatment were lower than that before treatment (all P<0.05). Conclusions The inducements of such an episode of mass hysteria are witness of fighting and the overwhelming anxiety about self-security. Disscoiative disorders mixed with conversion disorders is the major clinical feature. In order to control the episode of mass hysteria, the priority task is to relieve people’s anxiety.

BACKGROUND:Hot-melt adhesive is safe and environmental friendly adhesive due to free of solvent, which is particularly suitable for medical applications. OBJECTIVE:To describe the types and characteristics of currently used hot-melt adhesives and to prospect the technical research and development of hot-melt adhesive for polyolefin tubes or catheters as wel as to point out the corresponding key points to the hot-melt adhesion. METHODS:Literature search was carried out in SCI, Elsevier, and CNKI with the key words of“hot melt adhesive, medical application”in English and Chinese, respectively, for the initial retrieval of relevant articles or patents published January 1995 to December 2015. RESULTS AND CONCLUSION:To date, the medical hot-melt adhesives reported in the literature could be grouped as amorphous polyolefin, thermoplastic elastomer, acrylic and polyurethane types. The heat resistance of the most of the hot-melt adhesives does not meet the requirement for steam sterilizing process. In the present review, a strategy is proposed to develop a novel hot-melt adhesive which is good for binding polyolefin parts and can undergo the sterilization process. Given this, it is essential to choose a kind of polypropylene random copolymer with a suitable melting point as a substrate. With the aid of an adjuvant agent, therefore, we can develop a novel hot-melt adhesive that exhibits a lower melting point than the polyolefin tube, withstands steam sterilization temperature to ensure that the tube is not deformed during melt adhesion and is not become invalid during sterilization.

ObjectiveTo observe the dynamics of antibodies and protection against Schistosoma japonicum infections in buffaloes after immunized with recombinant 26 kDa glutathione S transferase (reSjc26GST). Methods Buffaloes in 2 villages endemic for schistosomiasis japonica were selected as test and control groups, respectively.In test group initially 96 buffaloes were vaccinated with reSjc26GST, and 90 buffaloes in the control group did not experience vaccination. The indicators included levels of antibodies to reSjc26GST in buffaloes before and after infection with S japonicum and changes in infection rate. Results Specific antibodies, which showed a trend of trapezoid increase, were induced in buffaloes after immunized with reSjc26GST. Twenty months after immunization, the infection rate of the test group was decreased by 62 2% when compared with that before vaccination,and by 67 7% when compared with that of the control in the corresponding period.Conclusion Specific antibodies and a certain extent of protection were induced in buffaloes after immunized with reSjc26GST, which played an significant role in ameliorating morbidity.

Objective To investigate the incidence and influencing factors of urinary incontinence in elderly patients with ambulatory care, and to provide basis for further improving the nursing of elderly patients with urinary incontinence. Methods A questionnaire survey was conducted among 970 elderly hospitalized patients aged over 60 years in 24 hospitals of 10 cities in Southwest China by the international advisory committee on urinary incontinence ( ICI-Q-SF) . According to the questionnaires we had known the incidence about urinary incontinence of the elderly ambulatory inpatients and analyzed the relationship between some factors ( age, sex, marriage, self-perception of health assessment, toilet capacity and so on ) and urinary incontinence. Results This investigation shows that the incidence about urinary incontinence of the elderly ambulatory inpatients was 20. 5%. The severity of symptom was divided into 3 grades: mild, moderate and severe, the incidence were 58. 2%, 30. 2% and 11. 6%. Regression analysis showed that the high incidence of urinary incontinence occurred in these inpatients who was older, mateless, poor self-evaluation and poor toilet capacity (P<0. 05). Conclusions Elderly ambulatory inpatients are the people at high risks of urinary incontinence. This is affected by many factors, so we should strengthen the nursing intervention for this kind of patients.

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
Animal Structures/chemistry ; Animals ; Antibodies, Helminth/blood ; Antigens, Helminth/chemistry/*genetics/immunology/*isolation & purification ; Cloning, Molecular ; Dirofilaria immitis/chemistry/*genetics/immunology ; Disease Models, Animal ; Dogs ; Escherichia coli/genetics ; Gene Expression ; Molecular Sequence Data ; Molecular Weight ; Recombinant Fusion Proteins/chemistry/genetics/immunology/isolation & purification ; Sequence Analysis, DNA ; Tumor Markers, Biological/chemistry/*genetics/immunology/*isolation & purification