OBJECTIVE To investigate the breathing circuit bacteria and the lower respiratory airway bacteria(LRAB) isolated from mechanical ventilation patient. METHODS A total of 48 strains of the breathing circuit and 87 strains of LRAB from mechanical ventilation patients were analyzed. RESULTS The main organism in the breathing circuit were Pseudomonas aeruginosa(31.2%),and Stenotrophomonas maltophilia(27.2%).The main organisms in LRAB were P.aeruginosa(37.9%),Klebsiella pneumoniae(17.3%) and Candida albicans(13.7%).The pathogens of the breathing circuit were 73.3 % similar with those of LRAB. CONCLUSIONS To control infection effectively,effective sterilizing measures must be carried out,especially attention to sterilizing and managements of the breathing circuit.

OBJECTIVE To explore the nursing intervention of lower respiratory airway bacteria(LRAB) isolated from patients with mechanical ventilation.METHODS A total of 72 stains of LRAB were isolated from mechanical ventilation patient and nursing intervention were analyzed.RESULTS The major pathogenic bacteria were Pseudomonas aeruginosa(22.3%),Acinetobacter baumannii(18.1%),Staphylococcus aureus(16.7%).CONCLUSIONS To control infection effectively,effective sterizing measures must be carried out,especially attention to EICU management,sterilizing of the breathing circuit and management of artificial respiratory airway.

Objective To investigate the influence of symptom incidence,severity,symptom clusters and related interference on the daily activities of colorectal cancer patients during the chemotherapy.Methods A sample of 112 colorectal cancer patients during the chemotherapy has been presented by M.D.Anderson Symptom Inventory (MDASI-GI) of Chinese Version.Exploratory factor and regression analysis are utilized to discuss influence of symptoms on the daily activities of patients.Results For patients with colorectal cancer after chemotherapy,the high incidence of symptoms are successively fatigue [99.1％ (111/112)],sadness [98.2％ (110/112)] and changes in taste [97.3％ (109/112)].Meanwhile,the severe symptoms are successively fatigue [92.8％ (104/112)],disturbed sleep [92.0％ (103/112)] and dry mouth.Furthermore,four symptom clusters are identified as upper-gastrointestinal symptom cluster,emotional symptom cluster,fatigue-related symptom cluster and excretion-related symptom cluster which possess statistical significance to the daily activities of patients (R2=0.782,F=96.071,P＜0.01).They were effective predictors factors of symptom distress.Conclusions Due to high relapse rate and severe condition,colorectal cancer patients during chemotherapy suffer from multiple symptoms which affect seriously daily activities of patients.Consequently,Medical staff should give prompt and accurate assessment,and then take specific intervening measures to alleviate the patients' symptom so as to improve patients' living conditions.

Objective To investigate health behavior of senile patients with hypertension and influencing factors.Methods Using general data scale,health conception scale,self-rated abilities for health practice scale and health-promoting lifestyle profile,206 patients with hypertension in the clinic and inpatient department were selected by convenience sampling method to investigate their health behavior.Results The score of health behavior of senile patients with hypertension was (72.25±16.66),23.8％ of patients were at high level.Among the subscale of health-promoting lifestyle profile,the score of nutrition was the highest,activity and healthy responsibility were the lowest.The score of health promotion was positively correlated with health conception,self-efficacy and education level,while negatively correlated with unhealthy habits.Conclusions Education about activity and healthy responsibility should be enhanced,we should set up right health conception,develop effective measures to enhance self-efficacy in order to improve health behavior of older patients with hypertension.

Objective To learn the roles of Helicobater pylori(Hp) colonization in shimofuri lesion formation of degestive tract and its transformation.Method Infective rate of Hp in biopsy specimens of gastric antrum and duodenum,degree of inflammation of the duodenal bulb mucosas were measured,and analyzed relationship of these indexes with Hp colonization in duodenal bulb mucosas,all of patients with shimofuri lesion were randomly divided into ranitidine suppressing acid group(ranitidine group) and anti-Hp therapy group(anti-Hp group),then two groups were secondly observed with gastroscope.Results The positive rate of Hp colonization in duodenal bulb was significantly difference in various phases of the disease(P

Rheumatoid arthritis(RA) is an autoimmune disease(AID) characterized by arthrosynovitis.Cytokines are involved in the whole course of the disease.Such important cytokines as TNF-? and IL-1? play a key role in the proliferation of synovial cells and progression of inflammation,and,therefore,have been taken as clinical therapeutic targets for RA in the recent years.The blocking agent of TNF-? or IL-1? is effective for only a few patients and the increasing adverse events it causes,such as infection and malignancy,are arousing more and more concern.The abnormal expression and imbalance of interleukin-6(IL-6) are closely associated with RA.IL-6 is an important regulatory factor for the pathogenesis of RA,and the blocking agent may be applied to the treatment of the disease.

Objective: To study 63 cases of nasal tumors treated by dermatologic surgery, and analyze their generality and characters. Methods: The cases were analyzed with SPSS 10.0 and SAS6.12. Results: The average age of the patients was 46.92?18.63.The number of man was about equal to that of women. Most cases were basal cell carcinoma (42.9%). Nevus came second (38.1%). The other cases were benign tumors. The distribution of operational locations showed the character of nasal surgery. Operations of Alar occupied 52.4%,22.2% were on the dorsal and 15.9% on the tip. Other locations occupied 9.5%. Cases of primary close occupied 69.8%. Cases applying complex close were 30.2%. Statistic analysis illustrated that primary close was different from complex close with distribution of operational locations. The area of defects between primary close and complex close was also different. At the same time, the differentiation of applying complex close was obvious between malignant tumors and benign tumors. Conclusion: Skin cancers and benign tumors usually occur on the nose, so it is very important to master the techniques of plastic surgery to repair the defects of nose, besides removing tumors perfectly by the classical way of dermatologic surgery, Mohs Micrographic Surgery.

Objective To develop a method for the determination of acrylamide in flocculant by gas chromatography-mass spectrometry.Methods Acrylamide was derived through bromination and extracted by organic solvent.GC-MS method was used to identify the target substance and quantify acrylamide.Results The recoveries obtained from samples spiked with standards were in the range of 70.2%-109.2% and relative standard deviations (n=5) was less than 5.0%.Linearity of peak area was obtained over a wide range of concentration (4.3-2 000 ?g/ml),and the correlation coefficients was 0.999 4.Conclusion The method is reliable,selective and reproducible and it is applicable to the determination of the acrylamide in flocculant.

Objective To investigate the relationship between IL-6-174C/G and-572C/G site gene polymorphism with nonvalvular atrial fibrillation(NVAF) as well as the national differences of interleukin-gene polymorphism between Kazak and Han nationality in Xinjiang region.Methods Seventy-nine Kazak patients of NVAF(Kazak NVAF group),78 Han patients with NVFA (Han NVAF group),75 Han cases of non-AF and 79 Kazak cases of non-AF were selected in Xinjiang region.The venous bloods were collected from extracting DNA.IL-6 gene-174G/C and-572C/G polymorphism in various groups were analyzed by adopting the PCR-RFLP technique.Results The allele frequency of IL-6-572G/C site G in the NVAF group was significantly higher than that in the control group(x2 =4.076,P＜0.05).The allele frequency of IL-6 gene-572 G/C site G had statistical difference between the NVAF group and the control group(OR=1.519,95 ％CI:I.087-2.122,P＜0.05),but the distribution difference between the Han and Kazak control groups had no statistical significance(P＞0.05);the advanced age ≥75 years old)(OR=6.468,95 ％CI:2.427-17.240) and left atrial dimension(OR =1.053,95 ％ CI:1.022-1.085) were the independent factors of AF occurrence;the left ventricular ejection fraction was a protective factor for preventing AF occurrence.The allele frequency of IL-6 gene 174G/C site C and G had no statistical difference among various groups(P＞0.05).Conclusion The allele of IL-6-572 G/C site G is a risk factor of genetic predisposition in NVAF.IL-6-174G/C site gene polymorphism has no relation with the susceptibility of AF.

OBJECTIVE:To study the centralized dispensing mode in outpatient pharmacy of large hospital METHODS:To establish the label dispensing system and management strategy RESULTS & CONCLUSION:Dispensing is the heart of a pharmacy Good networking system with efficient workflow and rational use of manpower could determine the success in a pharmacy,such as no dispensing error,no illegible handwriting,short wait-time automation of management of drug information and statistics of pharmacist's work load and rise of dispensing efficiency and quality of service