BACKGROUND: There was degeneration in the dermis only,but not necrosis completely after deep burn or mixed Degree Burns. After treatment with auto-skin grafting,the denatured dermis will recover to normal or close to normal morphology and structure. OBJECTIVE: To comparatively analyze the long-term effect of repair of deeply burned in function regions with large sheet of split-thickness auto-skin grafting with the preservation of denatured dermis or not during eschar shaving. DESIGN,TIME AND SETTING: A case analysis was performed in Department of Burnt Surgery of Dongguan Qingxi Hospital from March 2006 to June 2008. PATICIPANTS: Fifty-eight patients with deep burn in function regions were randomly divided into three groups according to different therapy ways. METHODS: The denatured dermis of patients in derma excised group was cut completely during eschar shaving and large sheet of split-thickness auto-skin was transplanted. The necrotic crust was cleared away with pare scab or scrape scabby method and reserved the denatured derma in derma reserved group. The patients who did not agree to operating skin transplanted on clinic served as control group,and they were treated with dressing change means till wound healing. MAIN OUTCOME MEASURES: Comparison of the bleeding volume in operation and scar proliferation after operating among the three groups,and evaluation the grade of the function. RESULTS: The bleeding volume in derma reserved group was less than that in dermis excised group during the operation. The survival rate of grafting skin in derma reserved group was lower than that in derma excised group. The shrinkage of grafting skin in derma excised group was more obvious than that in derma reserved group,and the long-term function would be worse than that in derma reserved group. The scar proliferation in control group was most obvious and the recovery of function was worst among the three groups. CONCLUSION: The good show and function was obtained to recovery of deep burn in function regions with large sheet of split-thickness auto-skin grafting and the preservation of denatured dermis during eschar shaving.

OBJECTIVETo detect serum interleukin-21 (IL-21) levels in patients with chronic hepatitis B receiving different therapeutic regimens.

METHODSA total of 198 patients with inactive chronic hepatitis B were divided into 3 groups according to the therapeutic regimens, namely interferon (IFN)-treated group (IFN group, n∓38), nucleoside analogue-treated group (NA group, n∓72) and untreated group (control group, n∓88). IL-21 and serum hepatitis B virus (HBV) markers were detected in these patients using enzyme-linked immunosorbent assay (ELISA), and the liver function indices were measured with an auto-biochemical analyzer.

RESULTSThe serum IL-21 levels in Con and IFN groups were significantly higher than those in NA group (102.29∓14.03, 123.01∓38.26, and 48.10∓7.06 pg/ml, respectively, P<0.05). When all the patients were regrouped according to the status of HBeAg, serum IL-21 level was 114.83∓19.88 pg/ml in HBeAg-negative group (n∓105), significantly higher than that of 61.53∓6.61 pg/ml in HBeAg-positive group (n∓93) (P<0.05). Bivariate correlation analysis showed no significant correlations between IL-21 and liver function indices.

CONCLUSIONThe immunomodulator IFN might be capable of increasing serum IL-21 levels, while nucleoside analogues can decrease IL-21 level in patients with chronic hepatitis B. HBeAg-negative patients have a significantly higher serum IL-21 level, suggesting that the expression of HBeAg might result in IL-21 depression.

Adult ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; drug therapy ; Humans ; Interferons ; therapeutic use ; Interleukins ; blood ; Male ; Nucleosides ; therapeutic use

OBJECTIVE: To establish a non-coated enzyme-linked immunosorbent assay (ELISA) based on zika virus envelope (E) protein for detecting the expression of E protein in infected cells. METHODS: Adherent Vero-143 cells infected with zika virus in a 96-well plate were fixed, and the antibodies against zika virus E protein were added at an optimized concentration to establish the non-coated ELISA method for E protein. The antiviral activities of lignans compound C1 was evaluated using this method. The accuracy of this non-coated ELISA was verified by RT-PCR, and the cross reaction with dengue virus was assessed. RESULTS: After optimization, the background absorbance at 450 nm of uninfected cells was reduced to about 0.20. The antiviral activities of lignans compound C1 detected by this method were basically consistent with the results of RT-PCR. No cross reaction with dengue virus was found in this assay. CONCLUSIONS A non- coated ELISA method based on zika virus E protein was established, which can be used for screening antiviral agents against zika virus.
Antibodies, Viral ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Viral Envelope Proteins ; Zika Virus ; Zika Virus Infection

Pigs are considered as ideal donors for xenotransplantation because they have many physiological and anatomical characteristics similar to human beings. However, antibody-mediated immunity, which includes both natural and induced antibody responses, is a major challenge for the success of pig-to-primate xenotransplantation. Various genetic modification methods help to tailor pigs to be appropriate donors for xenotransplantation. In this study, we applied transcription activator-like effector nuclease (TALEN) to knock out the porcine α-1, 3-galactosyltransferase gene GGTA1, which encodes Gal epitopes that induce hyperacute immune rejection in pig-to-human xenotransplantation. Meanwhile, human leukocyte antigen-G5 gene HLA-G5, which acts as an immunosuppressive factor, was co-transfected with TALEN into porcine fetal fibroblasts. The cell colonies of GGTA1 biallelic knockout with positive transgene for HLA-G5 were chosen as nuclear donors to generate genetic modified piglets through a single round of somatic cell nuclear transfer. As a result, we successfully obtained 20 modified piglets that were positive for GGTA1 knockout (GTKO) and half of them expressed the HLA-G5 protein. Gal epitopes on the cell membrane of GTKO/HLA-G5 piglets were completely absent. Western blotting and immunofluorescence showed that HLA-G5 was expressed in the modified piglets. Functionally, the fibroblasts from the GTKO/HLA-G5 piglets showed enhanced resistance to complement-mediated lysis ability compared with those from GTKO-only or wild-type pigs. These results indicate that the GTKO/HLA-G5 pigs could be a valuable donor model to facilitate laboratory studies and clinics for xenotransplantation.
Animals ; Animals, Genetically Modified ; Gene Knockout Techniques ; HLA Antigens ; Humans ; Nuclear Transfer Techniques ; Swine ; Transplantation, Heterologous