Aim To study the difference of proteins expression of epithelial cells and stroma of breast canc-er patients.Methods Formalin-fixed paraffin-embed-ded tissue samples from invasive ductal carcionma pa-tients were stained by improved HE staining methods. And the neoplastic epithelium and stroma were micro-dissected using laser capture microdissection and ana-lysed by mass spectrometry.Results Total 43 1 differ-ent proteins were detected by mass spectrometry and there were respectively 384 and 298 different kinds of proteins identified in epithelial cells and stroma.A-mong them,25 1 proteins were commonly expressed in two samples,while the contents of 69 and 60 proteins in stroma were respectively higher or lower than the ones in epithelial cells.The expression level and local-ization of proteins identified in epithelial cells and stro-ma were associated with their roles in development and progression of tumor cells.Conclusions The differen-tial expression proteins between epithelial cells and stroma of invasive ductal carcionma patients may func-tion as biomarkers for breast cancer screenning,diag-nosis and prognosis.

Objective To study the relationship between the MRI features of acute cerebral infarction (ACI) and developing vascular dementia (VD). Methods The patients who had ACI history more than 6 months were divided into vascular dementia group and no vascular dementia group by MMSE and CDR evaluations. The MRI data of acute stoke of the two groups were reviewed and analyzed retrospectively. Results In VD group the happenings of infarction at subcortex of lobus frontalis, subcortex of lobus temporalis, anterior capsula interna, knee of capsula interna, nucleus caudatus and thalamus were more than those in no vascular dementia group (P

Objective To investigate the association between the polymorphisms of brain-derived neurotrophic factor(BDNF) gene C270T and sporadic Alzheimer's disease(SAD) in Han Chinese.Methods The polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP) was used to detect BDNF gene C270T genotype and allele frequencies in 55 SAD patients and 80 age-and sex-matched healthy people(as normal control group).Results The frequencies distribution of BDNF gene C270T genotype and allele in the normal control group were suit to Hardy-Weinberg equilibrium(?2=0.167,P=0.682).The frequencies distribution of genotype and allele in the SAD group[C/C:52(94.5%),C/T:3(5.5%) and C:97.27%,T:2.73%]and normal control group[C/C:74(92.5%),C/T:6(7.5%) and C:96.25%,T:3.75%] were no statistical differences.Conclusion There is no association between the polymorphisms of BDNF gene C270T and SAD in Han Chinese.

Objectives A rare case of juvenile dermatomyositis (JDM) complicated by myelodysplastic syndrome (MDS) was reported in order to improve the understanding of the disease in its clinical signs, differential diagnosis and treatment. Meth-ods The course of the disease, clinical characteristics and the process of diagnosis and treatment were analyzed. Specimens of bone marrow, lymph nodes, muscles were examined by histopathological or immunohistopathological means and lfow cytometry. Results The diagnosis of JDM complicated by MDS was made based on the clinical manifestations, related laboratory results and the accompanying changes in hematological and bone marrow examinations. Conclusions JDM complicated by MDS is very special and rarely seen in China and its pathological mechanism and strategy of diagnosis and treatment should be further explored.

Objective To investigate the influence of synergistic effect of estradiol and testosterone on the level of lipids and coagulation function in mice with hyperlipidemia .Methods We established a maouse model in hyperlipidmia , giving estradiol (1 μg/d), testosterone (7 μg/d) or estradiol combined testosterone (1 μg/d E2 +7Tμg/d), respectively.After 14 weeks, we collected the blood from the mice , separated the serum to detect the lipids level , separated the plasma to test the coagulation function .Result Compared with controls , after high-fat diet , the serum level of total cholesterol ( TC) , triglyceride ( TG) , high density lipoprotein ( HDL) were significantly increased , PT and APTT were shorter ( P<0.05 ).However, after treating with estradiol combined testosterone , compared with cases, the serum level of C, TG and HDL were lower and PT , APTT were longer ( P <0.05 ), had no significance with controls . Conclusion The synergistic effect of estradiol and testosterone can mediate the lipids , reduce the level of LDL , regulate the coagulation function , reduce the risk of coronary heart disease , and also provide a new strategy for hormone therapy in coronary heart disease .

Objective The impact of complement Clq on inflammation in beta amyloidstimulated microglia.Methods After the cultured BV-2 microglial cells were treated with 100mg/L beta-amyloid fibers (fAβs),some of them were given C1q,others wcrc given C1q and C1qA.Then,interleukin-6 (IL-6) and tumor necrosis factor α (TNF α) in the supernatant and cell lysate were determined by the sandwich ELISA.Results A significant increase in TNF-α started at giving 50 nmol/L C1q after 100 mg/L fAβs (F =1177.27,P＜ 0.05),while the release of TNF-α was significantly suppressed by using 50 nmol/L C1qA on basis of this(P＜0.05).The level of IL-6 showed no above change.Conclusions C1q may enhance the inflammation of Aβ-induced BV-2 microglia cells and TNF-α may play important role in this effect.

Objective To observe the changes of the quality of life in type 2 diabetes accompanying subclinical atherosclerosis and explore the effect of diabetic macrovascular complications in the quality of life.Methods One hundred and thiry-six type 2 diabetes were measured carotid intima media thickness (IMT) and then divided into AS group(n =51) and CON group(n =85).Two groups were examined with Special of Quality of Life for Diabetes Mellitus (DSQL).Plasma glucose,plasma insulin,glycosylated hemoglobin(HbA1c),high sensitivity C-Reactive Protein (hs-CRP) as well as insulin resistance index (HOMA-IR) and body mass index (BMI) were observed.Results The scores of DSQL and all domains had obvious difference between AS group and control group(P ＜0.05 orP ＜0.01) ;Relative to the control group the AS group was significantly increased BMI,HbA1c levels,hsCRP levels.The DSQL was associated with IMT,BMI,HbA1c,hsCRP,HOMA-IR.Conclusion The diabetic macrovascular compliations might result in impaired quality of life,which is associated with hyperglycemia,insulin resistance,inflammation,and central obesity.Psychotherapy and health education are very important for the improvement the DSQL in type 2 diabetic accompanying subclinical atherosclerosis.

Objective To study the value of medical thoracoscopy in the diagnosis of cryptogenic pleural effusions.Methods Medical thoracoscopy was performed in 106 patients with cryptogenic pleural effusion.Pleural biopsies were routinely performed.Results Pathologic diagnosis was obtained in 102 patients,with pleural metastasis of lung cancer in 28 cases,malignant pleural mesothelioma in 9 cases,malignant lymphoma in l case,pleural metastasis of other cancer in 3 cases,pleural tuberculosis in 40 cases,inflammation in 16 cases and sarcoidosis in 1 case.No abnormality was seen by medical thoracoscopy in 4 patients.Conclusions Medical thoracoscopy is a safe and well-tolerated procedure with high diagnostic accuracy to cryptogenic pleural effusion,and ideal pleural organ can be obtained under direct vision.The positive rate of diagnosis of pleural effusions is improved significantly by medical thoracoscopy.

Objective To provide a fixtures and feasible injection method for rat tail vein injection.Methods SD rats were randomly divided into A group and B group,thirty rats in each group.Rats in group A fixed by a simple and practical experimental rats fixtures.And rats in group B fixed by common plastic drink bottles.Then the tail vein injection experiment was conducted respectively.Results It took one people 31.2 seconds in group A and 33.1 seconds in group B to finish the experiment from capture to fix rats,and took one people 68.4 seconds in group A to finish the experiment from capture to finish the injection,while it couldn't finish in group B.It took two people 25.4 seconds in group A and 25.8 seconds in group B to finish the experiment from capture to fix rats,and took 63.7 seconds in group A and 85.6 seconds in group B to finish the experiment from capture to finish the injection.Conclusion The experimental rats fixtures can increase the success rate of rats tail vein injection,and shorten the injection time.It is a safe and effective method.

Objective To study the effects of butylphthalide (NBP) on memory and apoptosis related protein as well as neuronal pathology in hippocampus of vascular dementia (VD) rats. Methods VD model was generated by the permanent occlusion of bilateral common carotid arteries in SD rats to produce the forebran ischemia. Male SD rats were randomly allocated into sham-operation group, VD model group, NBP treatment group and nimodipine treatment group. The function of memory was tested by the Morris water maze. The neuronal pathological changes and the expression of Bcl-2 and Bax proteins in the hippocampus were observed with hematoxylin-eosin (HE) staining and immunohistochemical staining, respectively. Results The impaired memory of VD rats was proved by the lengthened mean escape latency [(78.79±21.93)vs.(16.96±7.44),P＜0.05] and the neuron in hippocampus was severely damaged. The decveased ratio of Bcl-2/Bax resulted from the overexpression of Bax proteins in VD model group versus the sham-operation group [(43.00±6.72)vs.(6.00±1.29),P＜0.05]. The treatment of NBP notably improved the memory function of VD rats and reduced the hippocampus pathological injury (P＜0.05). The expression of Bcl-2 protein raised [(33.14±8.05)vs.(21.81±4.97),P＜0.05] along with reduced expression of Bax protein [(32.93±4.99)vs.(43.00±6.72),P＜0.05] after NBP treatment. However, there was no significant difference in the treatment effects between nimodipine and NBP group (P＞0.05). Conclusions NBP treatment could improve memory of VD rats and reduce the hippocampus pathological lesion by inhibiting the apoptosis related protein.