Objective To investigate the effect of intracerebroventricular injection of morphine and orphanin FQ (OFQ) on somatosensory evoked potential (SEP) and Na+-K+ATPase activity in cerebral cortex of rat. MethodsTo study the SEP with the BL-420 biological signal collecting system. The cerebral cortex tissues were extracted, homogenized and centrifuged. Na+-K+ATPase activity was measured with ATPase analytical kit. Results After intracerebroventricular injection with OFQ 0.9 ?g, the amplitude of P1-N1 and N1-P2 increased significantly(P

Objective To optimize the procedure of extracting components from Sophora alopecurcurodies ( Kudouzi) . Methods After external use of the extracts of Kudouzi by four different extraction proce-dures on the footpad inflammation rat model induced by carrageenan, the swelling rate was observed as the therapeutic index. The contents of oxysop hocarpine and oxymatrine of extracts from Kudouzi, as the two chemical indexes, were measured by using HPLC. The extraction procedure was screened by thera-peutic index combined with chemical indexes. Then the parameters of the procedure screened were opti-mized by orthogonal test. Results All the extracts inhibited footpad swelling in rats, and the effect of the ethanol extract was the strongest, and in which the contents of oxysop hocarpine and oxymatrine were the highest. The footpad swelling rate was negatively correlated with the contents of the two chemical in-dexes. The optimized extraction procedure was obtained with 10 times of 80%( V/V) alcohol, 4 hours at 50℃. Conclusion The extraction procedure of Kudouzi was stable and feasible, and the contents of ac-tive components were positively correlated with their effects.

Objective To explore the clinical effect of training assisted by a lower limb rehabilitation robot on the bladder and intestinal function of paraplegic spinal cord injury survivors. Methods Thirty-eight paraplegic patients with spinal cord injury were divided according to their admission order into an experimental group ( n=19) and a control group (n=19). Both groups were given conventional rehabilitation training, while the experimental group was additionally provided with robot-assisted lower limb training in three stages:adaptation, training and con-solidation. It lasted 30 minutes daily, 5 days per week for 12 weeks. Before and after the training, an urodynamics examination system was used to evaluate the maximum urine flow, bladder capacity, residual urine volume, bladder pressure and detrusor pressure. Colon transit time, mean rectal pressure and intestinal function were measured using the colon transit test, a mean rectal pressure test, and the Functional Independence Measure ( FIM) scale respective-ly. Results The average bladder volume, maximum urine flow rate, average urine flow rate, detrusor pressure, bladder compliance, average rectal pressure and intestinal FIM score of the robot training group after training were all significantly better than before the training, as were the average residual urine volume and colon transit time. After the training, the average bladder volume, maximum urine flow rate, average urine flow rate, detrusor pressure, bladder compliance and average rectal pressure of the robot training group were all significantly higher than those of the control group, while the average residual urine volume and colon transit time were significantly smaller. Then, 32％ of the patients in the experimental group achieved no less than 6 points for their average FIM score, significantly higher than in the control group. Conclusion Robot-assisted lower limb training combined with comprehensive rehabilitation training can effectively improve the bladder and intestinal function of paraplegic patients after a spinal cord injury.