Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide.MicroRNAs (miRNAs) are a kind of endogenous non coding singlestranded RNAs,which can function as a tumor promoter or suppressor in the development and progression of human malignant tumors.Studies have shown that miRNAs play an important role in the occurrence and development of HCC.Numerous studies have found that the expression of miR-34a in various tumors is reduced and it participates in the carcinogenesis.Currently,the role of miR-34a in the tumorigenesis and development of HCC has attracted widespread attentions.Studies have confirmed that miR-34a can regulate the expression of its target genes and thus affect some key biological processes such as cell proliferation,apoptosis,invasion and metastasis of HCC.In this paper,we mainly reviewed the recent research progress on the role of miR34a in HCC.

Objective To explore the tumor markers for the diagnosis of primary liver carcinomas (PLC) by detecting the serum protein spectrum differently expressed between PLC patients and healthy controls. Methods We detected the serum protein spectrum in 50 PLC patients and 50 healthy controls using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique and find the significant protein peaks. The serum Alpha-fetoprotein (AFP) levels in all 100 serum samples were also measured by ELISA. Results The protein peaks, which could discriminate healthy individuals from PLC patients, were detected. Four protein molecules (3354.71, 8825.80, 4345.08, 13 715.01) had a significant difference between PLC patients and the normal controls (P <10-5), indicating that these protein molecules might be a potential marker for PLC. The specificity and sensitivity of SELDI-TOF-MS were 94% and 90% respectively. Sixteen PLC patients were AFP positive and the sensitivity was 54%(27/50). Conclusion With a high specificity and sensitivity, the detection of serum protein spectrum can be performed easily and quickly by SELDI-TOF-MS technique, which provides a serological way in identifying PLC and most likely to benefit from AFP strategies.

Objective:To determine the effects of Jinlong capsule combined with interventional therapy on the immune functions of primary hepatocellular carcinoma patients. Methods:Sixty randomly selected cases of clinically diagnosed primary hepatocellular carcinoma were divided into the observation group and the control group. Three days after operation, the observation group was given four Jinlong capsules three times a day for 30 days (one treatment). Meanwhile, the control group received interventional therapy after the operation. One to four days following one treatment, peripheral blood specimens were collected from the two groups to determine the cellular immune function indices. Results:The cell numbers (mean) of the peripheral blood components CD3, CD4, NK, SIL-2R, TSGF, and SIL-2R and the CD4/CD8 ratio in the observation group showed no significant difference before and after treatment. In the control group, these indices were significantly different before and after treatment. Conclusion:The Jinlong capsule facilitates the cellu-lar immunity recovery of patients with primary hepatocellular carcinoma after interventional therapy.

Objective To explore status and influencing factors of nurses′ satisfaction with grading management in a level three hospital. Methods The plan of grading management was established which involved the defining nurses′ qualifications at all levels, establishing the evaluation index system of nurses grading, performing APN arranges, confirming the competition procedure. A total of 250 nurses were investigated by a questionnaire according to convenient sampling method. Results A total of 250 questionnaires were delivered and 236 of them were collected with the effective response rate of 94. 4%. Nurses were satisfied with grading management. Influence factors of nurses′ satisfaction with grading management were age and education level. Conclusions It is suggested that nursing managers further optimize grading management model by considering many factors in order to improve the nurses′satisfaction.

Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide.At present,the molecular mechanism of the carcinogenesis and development of HCC have not been systematically elucidated.Silent information regulator 1 (SIRT1) is a histone deacetylase,which is dependent on nicotinamide adenine dinucleotide (NAD+).It is involved in many biological processes such as metabolism,oxidative stress,genotoxicity,aging and tumor formation.During the recent years,thefunction of SIRT1 in the carcinogenesis and progress of HCC has attracted wide attention.SIRT1 is abnormally expressed in the development of HCC,and acts as an oncogene.It promotes the proliferation,apoptosis,metastasis and chemotherapy resistance of HCC through multiple molecular pathways including microRNA,AMP-activated protein kinase,Yes-associated protein,telomerase,autophagy,PI3K/Akt and so on.In this article,we discussed the latest research progress of SIRT1 in HCC.