Many inorganic salts were studied for their effect on the ascorbic acid content of Mung bean sprout. With the view of the possible application of the experimental results on large scale bean sprout production, the chemicals studied were dissolved in the tap water used for soaking and daily flushing the bean during sprouting. The relation between temperature, length of the sprout and ascorbic acid content were also observed.It was found that Co(NO3)2, at a concentration of 0.001 M promoted ascorbic acid synthesis; and NH4NO3 at 0.01 M hastened the growth of the sprout. Other substances studied either exerted no positive effect on ascorbic acid production or depressed the growing of the sprout. When the temperature was between 10-15℃ and the length of the sprouts were 2 cm, ascorbic acid production was most favorable.

The objective of this study is to evaluate the relative bioavailabilities (RBA) of iron in Soybean meal and egg yolk powder by means of the rat hemoglobin repletion method, and to analyse their iron contents by the atomic absorption spectrometric determination.The iron content of soybean meal is 159.78 ppm, RBA is 67.24%; the iron content of egg yolk powder is 128.65ppm, RBA is 70.90%. This means that both soybean and egg yolk are of high iron contents, and their RBAs are not too inferior, indicating that the absolute amounts of available iron are still high. So both of them should be considered a rather good source of dietary iron.Using the total iron consumption and the final total hemoglobin of each animal during the hemoglobin repletion period as a dose-response index to calculate the RBA is thought to be reasonable.

The Present study designed according to AOAC is conducted to evaluate the practical protein quality of the porcine blood solely and mixed with other dietary proteins.Proximate analysis shows the average contents of crude protein and water in the spray-drying porcine blood are 85.0% and 12.0% respectively.The porcine blood protein is scant of Isoleucine (0.5%) and its Leu/Ile ratio is as high as 26. The amino acid pattern results in poor protein quality of the porcine blood, with BV, NPU and NPR value being only 16.5, 15.5 and 0 respectively. Therefore diets containing porcine blood as a sole source of protein can not maintain the normal body weight of rats.But porcine whole blood protein is rich in Lysine (7.8%) . When the spray-drying porcine blood is mixed with the wheat flour in proportion of 3%, the protein quality of the mixed dietary protein comparing with either of its constitutional proteins, is improved remarkably and its nutritive parameter values (BV:73.3, NPU: 61.8, PER: 2.46, Calbrated PER:1.9 and NPR: 4.3 respectively) are higher than those of the wheat flour protein (BV: 55.3, NPU: 46.6, PER: 1.01, Calibrated PER: 0.8 and NPR: 3.1 respectively).

In order to evaluate appropriately the role of lipid metabolism by the refined konjac meal (RKM), a new source of dietary fiber, the effects of RKM on the level of serum and liver lipids, hepatic histopathology and on the composition and output of fecal bile acids in comparison with pectin, algin and agar were studied.The RKM contained about 75-80% of glucomannan. All four kinds of dietary fiber "were given at a level of 5% in the diet to the young male Wistar rats fed on a lipid-rich diet containing 5% lard, 1% cholesterol and 0.25% chplate. The control group was fed on a lipid-rich diet alone withput fiber. The normal group was fed on a basal diet without extra lipid added. Feeding period was 9 weeks.The results obtained were as follows;1)All the dietary fibers tested have the similar effects on serum lipid composition. At the end of 2-week of the experiment, the serum total cholesterol levels of the four experimental groups were only 62% to 75% of that of the control group, and at the end of 7-week they were lowered to the same level of the normal group. The level of the triglyceride in serum did not change in all groups during the experiment.2)Relative liver weights were lower in four experimental groups than in control group. Hepatic hisiopathological examinations showed lipotropic effect in RKM, pectin and algin groups. Both of the above effects appeared most significantly in RKM group. The concentrations of total cholesterol and triglyceride in liver in RKM group were lower than those in control group as well as other three experimental groups.3)No harmful effects on the intake of food and growing of rats were found when feeding these four kinds of dietary fiber at the level of 5%.4)The daily output of fecal bile acids (CDCA + GDCA) was significantly increased in four experimental groups.The increase of fecal output of bile acids, especially CDCA, may be one of the mechanisms of hypocholesterola-emic effect of dietary fibers.It could be concluded that RKM has the same significant hypocholestero-lemic effect as the pectin, algin and agar, but its lipotropic effect is more potent.

Objective To study the distribution of the gap conjunction and connexin(Cx)26 and 32 in inner ear of guinea pig. Methods We investigated expression and distribution of gap conjunction and Cx 26 and Cx 32 in cochlea of guinea by immunohistochemistry, RT-PCR and electron microscopy. Results T The gap conjunction was easily seen between hair cells and supporting cells in cochlea of guinea pigs. Cx26 was widely distributed in the stria vascularis, the spiral ligament and between the supporting cells, and Cx32 appeared mainly among supporting cells, in the stria vascularris. Conclusion There exist lots of gap conjunctions in inner ear of guinea pig. Two kinds of connexins can be seen in guinea pig's cochlea, and the Cx 26 plays more important role than other Cx.

Although breast feeding has long been proved to be the best way tor infant feeding, for many reasons, however, the number of breast-fed infants were always fewer than those of artiticially or mixed fed ones either in urban or in rural, e.g. it was found that only 35.7% of infants aged 4 months in Shanghai were breast-fed in 1980. Therefore manufacturing some kinds of humanized milk for improving infant nutrition seems necessary.A humanized milk powder was manufactured in Hongyuan Powdered Milk Factory. For testing its suitability for infant nutrition, a feeding experiment in infants was conducted in our laboratory in spring 1982.75 healthy babies aged 1-5 months were equally randomly assigned into three groups, i. e. humanized milk group, breast feeding group, and cow's milk group. The other food supplements except the milks were same in all groups.All the babies were subjected to anthropometric examination and blood hemoglobin determination before and after the 3 months' experimental period.Experimental results showed that there was no significant difference between the three groups either in growth and development or in blood hemoglobin level.In addition, Hongyuan humanized milk powder was also found to be more digestible and acceptable for the majority of the tested infants.

Samples of the soybean and mung bean sprout were obtained bimonthly throughout the year from market as well as directly from the top and middle layers of the sprouting vessels of the producers. The contents of moisture and ascorbic acid were determined.Although the temperature of the environment varies from 4-25℃, that of the middle layers of most sprouting vessels varies within a narrow range of 20-28℃.The ascorbic acid contents of both bean sprouts obtained at different month of the year show no statistical significance of variation. The average value of 9.65 mg % for soybean sprout is significantly lower than that of mung bean sprout, 13.34 mg %.Samples of both bean sprouts obtained from the top layer of the sprout vessel are richer in the vitamin content than those obtained from the middle layer. This is attributed to the difference of oxygen supply.