The standard ofClassificationandDetermination of Physical Constitutions in Traditional Chinese Medicine (TCM), which was issued by the China Association of TCM in 2009, has been promoted and implemented. However, we found that the criteria were inconsistent with the principles and methods of constitution classification in TCM after serious research. The features for physical constitution classification were repetitive and redundant without clear boundaries among them. Furthermore, there were poor correlations among different factors within a single physical constitution. Accordingly, it was suggested that the theory of physical constitutions in TCM should not be confused with and treated as the science of it. Because the standard ofClassiifcationandDetermination of Physical Constitutions in TCM is not mature enough, we should be cautious when applying it.

Objective:To approach the potential rules ofsyndrome differentiation and medication for diabetes by ancient medical scholars.Methods:By DBMS(Data Base Management System) ofPuji Fang, the information about diabetes and its correlated disease was collected, such as kinds ofthe drugs, application times ofdrugs and the number ofprescription.The qualitative analysis and quantitive analysis were used to ascertain the optimal and common used drugs and pairs ofdrugs for diabetes, and to deduce the potential indication ofdiabetes in ancient times.Results:The most optimal drugs used for diabetes in ancient times were DwarfLilyturfTuber, trichosanthis, Ginseng and Indian Bread.The common used pairs ofdrugs were DwarfLilyturfTuber and trichosanthis, DwarfLilyturfTuber and Ginseng, trichosanthis and Golden Thread, Ginseng and Indian Bread.So we can deduce that the potential indications ofdiabetes were impairment ofbody fluid by dry and heat, and deficiency ofboth qi and yin.Conclusion:By integrated logic analysis and statistical analysis, we can reveal the ancient treatment rule ofthe disease and it provided reference for clinic today.

There was severe logical contradiction among the basic concepts in Chinese medicine such as syndrome, symptoms and signs, etiopathogenisis and pathogenesis .In this paper, from the comparative reseaech of the different versions of Basic Theory of Traditional Chinese Medicine (TCM) used in higher colleges and universities of TCM in recent 50 years,higher Colleges of Chinese medicine materials, it cleared up the evolution of the basic connotation of syndrome, symptoms and signs, pathogens and pathogenesis, and revealed fundamental reason for the occurance of logical contradiction.

Pathogenesis theory occupies an important position in TCM theoretical system. Suwen is the pioneer. Enriched by later generations,the traditional pathogenesis has been a significant change. It causes understandable bias to their initial implications and affects modern research. In this paper,based on Suwen's academic background,it discusses the composition,completeness and the basis to confirm Pathogenesis 19,offering some new ideas.

Objective:To study potential function of relieving-pain,calming and stopping bleeding function of astragali.Methods:To observe the effect of the water decoction of high,middle and low doses astragali on physical pain(hot plate method) and chemical-induced pain(writhing method)in mice reaction;the effect on the sleep durance of mouse which was applied under valve dosage of sodium pentobarbital and impact of blood coagulation in rats.It was compared with the blank control group,as well as the positive control group.Results:Astragali decoction can increase the pain threshold in mice,reduce the number of writhing in mice,enhance the volume of the role of sleep in mice on the threshold of sodium pentobarbital hypnosis,and extend the role of sodium pentobarbital hypnosis time.At the same time can shorten the prothrombin time and partial thromboplastin time,and increase fibrinogen.Conclusion:Astragali had definite relieving-pain,calming and stopping bleeding function.This result was consistent with the analysis conclusions through ancient prescriptions database with astragali.

To investigate the relationship between diabetes and its names in ancient times by analytical method of medical theory,comparison of medication rules and statistical analysis method.And then foundation will be established for exploring the ancient treatment rules of diabetes.The comparison and mutual reference of the above three methods can reveal disease essence and their mutual relationship in the different levels,which had an important technology signifi cance.

Objective To establish intellectual critique of nursing thesis course in postgraduate nurse,and to evaluate teaching effectiveness and the reasonableness of the course.Methods A total of 44 postgraduate nurses were involved in the study,which were carried out through action research of planning,acting and observing,and reflecting.After the course,researchers evaluated the reasonableness of the course and teaching effectiveness through the self-control.Results There was significant difference in the scientific research ability score of research object before and after the course:(60.09±16.26) scores vs.(71.27±13.22) scores,t=-3.264,P＜0.01.But there was no significant difference in the critical thinking ability scores of research object before and after the course:(295.55±29.71) scores vs.(292.71±26.67) scores,t=0.478,P＞0.05.Conclusions The course of intellectual critique of nursing thesis can improve the research ability of postgraduate nurse,but the role of the critical thinking ability remains to be discussed.And the course needs to be further improved.

Objective To explore the aesthetic results of medial upper Iip repair for skin and soft tissue defects using local flap.Methods According to the location and the size of upper lip defect,the modified rhomboid flap of 60 ° above the defect was designed to repair the wound in philtrum;the O-L flap along philtral column to vermilion border was designed to cover the wound close to the peak and lateral to philtral column.Results Twenty-one postoperative patients that underwent pigmented nevus removal (12 female,9 male;ages 16-33 years) had defects in philtrum for 10 cases and close to the peak and philtral column for 11 cases.The diameter of the defect ranged from 0.5 cm to 1.0 cm.All flaps survived primarily without any complications and follow-up was for 1 to 18 months with excellent outcomes in all cases.All patients were satisfied with contour and functions.No pigmented nevus recurrence occurred.Conclusions The flap designed according to the aesthetic principle could be used to repair medial upper lip defect of medium-sized,which not only obtains functional reconstruction,but also satisfies aesthetic results,with aesthetic subunits saved and scar concealed.

Objective To investigate the role of T-type calcium channel in lidocaine-induced neuronal cytotoxicity . Methods SH-SYSY cell line was a gift from cell biology laboratory of our medical university. The cells were cultured in DMEM liquid culture medium at 37℃ in incubator filled with 5% CO2 , and randomly divided into 4 groups ( n = 66 each) : control group (group C)and M, L and ML groups were exposed to 5 μmol/L mibefradil (a T-type calcium channel blocker), 10 mmol/L lidocaine and 5 μmoL/L mibefradil + 10 mmol/L lidocaine for 24 h. Cell morphology was examined by electronic microscopy at 24 h of drug exposure. Cell viability (by MTT) and neuronal apoptosis (by flow cytometry) were detected immediately before and at 1, 6, 12 and 24 h of exposure to mibefradil or/and lidocaine.Results In C and M groups, the cells demonstrated dendritic protrusions, enlarged nerve processes and dense lattice. After being exposed to lidocaine for 24 h, the dendritic protrusions disappeared,the cells decreased in size, shrinked and became round; the cell viability was significantly decreased while the neuronal apoptosis increased. The lidocaine-induced changes were significantly attenuated by co-incubation with mibefradil. ConclusionT-type calcium channel is involved in lidocaine-induced neuronal cytotoxicity.

Objective To explore a new methodology with bilateral rhomboid dermal flaps for surgical treatment of inverted nipple. Methods 36 cases with 64 inverted nipples were involved in this study. Among these cases, 8 of them were evaluated as mild degree, while 19 moderate and 9 severe. In this procedure, the bilateral rhomboid incisions were designed. The rhomboid skin was deepithelialized and two dermal flaps were created. Then these two dermal flaps were filled crosswise through the tunnel beneath the nipple and fixed as supporting. After the operation, the continuous traction of the nipple lasted for one week, and sutured out 10 days later. Results None of these 36 cases appeared nipple blood circulation disorder after operation. Postoperative follow-up for 6 months-3 years showed that the appearance of the nipple was satisfactory. There were no recurrence and sensory disturbance. Conclusions The method introduced in this study is simple, the appearance of nipple is natural and no complication has been found. The incision is larvaceous while the scar is invisible. Meanwhile this method is also benefit for reserving the function of breast-feeding. It is proved to be an ideal method for correcting the inverted nipple.