Objective To study the clinical effect of shenfu injection on ultrasound cardiogram in patients of acute myocardial infarction combined with pump failure.Methods Eighty patients with acute myocardial infarction combined with pump failure were divided into control group and experiment group by random digits table method with 40 cases each.The patients in control group received common drug treatment,and the patients in experiment group received common drug combined with shenfu injection treatment.The clinical effect and the change of ultrasound cardiogram after treatment were compared between the 2 groups.Results The total effective rate in experiment group was significantly higher than that in control group [82.5％ (33/40) vs.50.0％ (20/40)],and there was statistical difference (P ＜ 0.05).The results of ultrasound cardiogram showed there were no statistical differences in peak E velocity,left ventricular ejection fraction,left ventricular fractional shortening and E/A before treatment between the 2 groups (P ＞0.05),and the indexes were significantly improved after treatment compared with those before treatment.Furthermore,the indexes in experiment group were significantly better than those in control group [(0.87 ± 0.18) m/s vs.(0.65 ± 0.22) m/s,(51.0 ± 10.2)％ vs.(43.9 ± 12.1)％,(27.5 ± 6.5)％ vs.(22.5 ± 5.3)％,0.86 ±0.18 vs.0.76 ±0.13],and there were statistical differences (P ＜0.05).Conclusion Shenfu injection may play an important role in improving prognosis,decreasing mortality for patients with acute myocardial infarction combined with pump failure.

This paper reports the observation on the distribution of succinic dehydrogenase of the cochlea in normal guinea pig. The method we adopted is as follows: The temporal bone was rapidly removed and the apical portion of the cochlea and the round window were perforated after decapitation under the temperature at 0? to 4℃. Incubation solution was infused into the apical portion of the cochlea and flowed out through the round window. The above procedure was carried out within 3 min. Then the specimen was put into incubation solution for 30 min at 37 C. The same infusion pathway from apical hole to round window was adopted, to introduce a cold, buffered formol sucrose solution for fixation. After first fixation by infusing, the cochlea in the fixative was subjected to a vacuum system for 20～30 min, and finally put into refrigerator to complete fixation. The specimen was then decalcified in 10 per cent EDTA, and immersed in 10 and 20 per cent gelation successively for embedding. The frozen sections was cut into 15? thick midmodiolar sections in a cryostat. This observation reveals that the inner and outer cell, the cell of spiral ganglion and the stria vascularis showed a very strong enzyme activity, while the tectorial membrane, the basilar membrane and other cells showed no activity. The results obtained and the methods are discussed.

The accomplishment of voluntary active euthanasia must involve the concept of "the other".The whole autonomy is an Abstract theoretical principle,which meets logic difficulty in real practice."The other" limits autonomy on the attitude towards life,family relationship,social relationship,physicians,and so on,which is also the practical basis for the accomplishment of euthanasia.When an individual chooses the will of performing euthanasia,he or she should abide by the principles of respect,communion and autonomy.These principles should also be followed in the future legislation of euthanasia.

Objective To investigate the effects of low doses 147Pm internal irradiation against hereditary mutation in mouse cells induced by subsequent high dose of 147Pm irradiation. Methods The frequencies of micronucleated polychromatic erythrocytes (mn-PCE) micronucleated normochromatic erythrocytes (mn-NCE) and chromosome aberration in bone marrow and peripheral blood cells were observed. Results The frequencies of nm-PCE、mn-NCE and chromosome aberration induced by high dose of 147Pm irradiation showed a highly significant increase over the control at 24 h after irradiation (P＜0.05 or 0.01).However, the frequencies in cells pre-exposed to 0.37-37 Bq/g of 147Pm were decreased significantly (P＜0.05 or 0.01),as compared with those of the group without pre-exposure. Conclusion Low doses of in vivo 147Pm β-irradiation can induce a radioadaptive response of the lymphocytes and erythroblasts.The induction of the adaptive response has a wide dose range.The mn-PCE and mn-NCE are more sensitive indexes for revealing biological effects of radiation as compared with the chromosome aberration.

Objective To analyze factors affecting the expenses of inpatients and their comprehensive force of influence so as to check irrational increases of medical expenses. Methods Data on hospitalization expenses for disease entities ranking in the first ten places in a third tier hospital in 1997 were taken as samples and a statistical analysis was conducted of the medical expenses incurred in the discharged cases by means of the nerve network model. Results Major factors affecting hospitalization expenses were days of hospitalization, pre hospitalization conditions, pre operation length of stay and age, the comprehensive force of influence of which was respectively 0 4879 , 0 2124 , 0 1847 and 0 0612 . Conclusion To check the irrational increases of medical expenses, we must focus our efforts on the reduction of expenses for medicine and shorten days of hospitalization and pre operation length of stay.

PurposeTo investigate the relationship between the TCD higher blood flow velocity and the pathology of internal cranial arteries,so as to improve the veracity of diagnosing cerebrovasculer diseases by TCD. MethodsComparing the blood flow velocity of 84 patients,whose cerebral blood flow are abnormal higher in one or above vessels detected by TCD,with the results of magnetic resonance angiography (MRA). ResultsCerebrovascular stricture,closeness and other diseases are discovered at different parts and to some extent in 80 of above 84 cases according to their detecting results of MRA. ConclusionIt is reliable to diagnose stricture,closeness and other diseases of internal cranial large arteries by TCD higher blood folw velocity.

Objective To evaluate the effects of micro-screw on the intrusion of lower incisors and the leveling of curve of Spee in the process of orthotherapy.Methods Fifteen patients (5 males,10 females) with deep-bite were selected,aged from 11.3 to 28.8 years,with average of 17.2 years.All of them were Angle Class Ⅱ Classification 1 malocclusion and had a curve of Spee larger than 3 mm.Orthotherapy of extraction was adopted for the first premolars of two upper mandible and second premolars of two lower mandible.All these patients had micro-screws implanted at root apex area between lower lateral incisor and canine bilaterally,and when the 0.457 mm× 0.635 mm (0.018 in ×0.025 in) Ni-Tisquare wire was internalized,the strength of intrusion from micro-screws was used.Lateral cephalometric radiographs were taken before loading the micro-screw and immediately when the curve of Spee was leveled.Two linear and two angular measurements were selected for cephalometric analysis.Results The following results were noted: the lower incisors were significantly intruded for a mean of (4.10±0.98) mm; the mandibular molars were not significantly extruded for a mean of (0.91±0.69) mm; some lower incisors proclined and some lower incisors retroclined for a mean of (-0.09±4.12)°.After bite opening,the mandibular plane angle was minimally altered for a mean of (0.25±0.70)°; the ratio of lower incisor intrusioin to lower molar extrusion was 4.5: 1.0.Conclusions The combined use of micro screw with Ni-Ti wire is capable of enhancing the intrusion of lower incisors and leveling the curve of Spee,and it has minimal side effects on the posterior teeth.

Objective To evaluate the expression of GPR30 and Ki-67 in Non-small cell lung cancer(NSCLC) and the relationship between them.The clinicopathological features of GPR30 in NSCLC were also analyzed.The molecular mechanism that estrogen mediated the proliferation of H1299 by activating GPR30 was further studied.Methods The expression of GPR30 and Ki-67 in 80 cases of specimens of NSCLC after surgery was examined using immunohistochemistry method.After 17-β-estradiol(E2) or G-1 added,H1299 cells were counted and the cell cycle distribution was analyzed by flow cytometry.Finally,the activated ERK1/2 and the expression of cyclin D1 and p16 after G-1 treatment in H1299 cells were examined through western blotting.Results Expressions of GPR30 was more in stage Ⅲ or low differentiation tissues or adenocarcinoma (P＜0.05).A positive correlation between GPR30 and Ki-67 was further disclosed (r=0.502,P=0.000).The proliferation of H1299 cells was promoted and more cells entered S-p hase after E2 or G-1 treatment for 3 days,which could be inhibited after G-15 or U0126 pre-treatment for 2 hours.We further discovered that the activated ERK1/2 and cyclin D1 expression increased after G-1 treatment,which was blocked after G-15 or U0126 pre-treatment for 2 hours.The change of p16 was on the opposite.Conclusion A positive correlation existed between GPR30 and Ki-67.GPR30-EGFR-MAPKs signaling transduction pathway was involved in the estrogen-induced proliferation of NSCLC cells.Blocking GPR30 signaling pathway may be a promising new strategy for NSCLC treatments.

Objective To explore the diagnostic value of contrast-enhanced spiral CT after low tension and drinking water in papillary carcinoma of ampulla. Methods CT manifestations of papillary carcinoma of ampulla comfirmed pathologically in 20 cases were analysed retrospectively emphasized in artery phase on contrast CT which was performed after injecting 654-2 20 mg and drinking 300~500 ml water.Results Of all the cases , only 2 cases showed tumor filling defect in the descending part of duedenum nearby pancreas on routine CT, but all the cases showed more or less tumor filling defect on contrast-enhanced spiral CT . The diameters of the tumor were between 0.8 to 2.6 cm,2 cases with head of pancreas affected,the diameters of the tumor were between 2.4 to 2.6 cm.There were 2 cases with lymph node metastasis nearby duedenum.All the cases showed expansion of gallbladder ,intrahepatic duct and choledochus expanding in the liver. Conclusion Contrast-enhanced spiral CT after low tension and drinking water is superior to routine CT in determining the size and morphosis of tumor.

Objective To study the protective effect of emodin on hepatic ischemia-reperfusion injury in the rat(liver). Methods Thirty Wistar rats were randomly devided into 3 groups:the sham-operatment group (Control group),the ischemia-reperfusion group (I/R group) and the emodin-treatment group (EMD group). By(clamping) the left and median lobes of the liver for 1 h and followed by 1 h of reperfusion,the(partial) (70%)(normothemic)(ischemia)-reperfusion injury model was established. The rats of emodin group received emodin [60mg/(kg?d)] by(oral) route for 3 days before ischemia. Blood and liver samples were obtained for(biochemical) and(histological) analysis 1 h after reperfusion. Some liver specimens were taken for flow cytometry(analysis) to assess the(mitochondrial)(membrane) potential (??m) . Rhodanmin123 and PI were used to label the cells.Results After reperfusion,(serum) levels of alanine transaminase(ALT) and liver contents of(malondialdehyde) (MDA) in the I/R group rats were increased significantly (P