Objective To observe the effect of leflunomide, combined tripterygium glycosides in treatment of nephrotic syndrome. Methods 64 patients with refractory nephrotic syndrome, according to drug using were divided into two groups,34 patients received the conventional therapy plus leflunomide combined tripterygium glycosides,30patients received conventional therapy alone, such as cyclophosphamide and prednisone,effects and follow-up were observed,as well as kidney function, blood lipid changes. Results After 30d treatment,the efficacy were observed. The results showed the efficiency of the observation group were significantly higher than control group. After 1 year followup,the recurrence rate of the observation group was significantly lower than the control group. 24h urine protein in both groups decreased significantly, ALB was higher than before treatment ,TG TCh was significantly lower than before treatment,and different indexes in the treatment group were significantly better than after the reduction and increase the level of the control group(P ＜ 0.05). Conclusion Lellunomide,tripterygium glycosides combined treatment of ne phrotic syndrome had good effect,and recurrence rate was low,and it could improve kidney function,blood fat,it was worthy of wide dissemination and application.

Objective To design a new portable vital signs monitor for family use. Methods TMS320F2812, a kind of digital signal processor, was applied as the main processor to control the functional mod-ules including A/D convertor, LCD display. Bluetooth communication module was introduced to achieve real-time fast transfer of data between the system and the PC. Results Not only can the system monitor electrocardiogram (ECG), breath rate, pulse rate, body temperature and other vital signs accurately, but also it runs stably. Conclusion The features of DSP and Bluetooth were well combined in the design of the family-oriented, easy to use, multi-functional monitor with small size, low power consumption and convenience. A broad application and market prospects can be predicted.

Objective To observe the efficacy and safety of safflower yellow injection in the treatment of sta-ble angina pectoris.Methods 70 patients with stable angina pectoris were selected and divided into observation group and control group by random number table method,35 cases in each group.The observation group used injection of safflower,and the control group used Xuesaitong.The effect was compared between the two groups.Results The total effective rate of the observation group was 94.3%,which was higher than 74.3% of the control group,the differ-ence was statistically significant(χ2 =5.285,P <0.01).The clinical indexes of the two groups after treatment were significantly improved than before treatment,the differences were statistically significant (t =13.021,25.407,3.376, 3.930,7.119,10.163,18.044,3.765,3.124,6.096,all P <0.05).The angina attack times of the observation group was less than that of the control group ,the dosage of nitroglycerin was lower than that of the control group ,the differences were statistically significant(t =4.686,6.568,all P <0.05).The incidence rate of adverse reaction was 5.7% in the observation group,compared with the control group of 8.6%,the difference was not statistically signifi-cant(χ2 =0.729,P >0.05).Conclusion The injection of safflower yellow pigment in the treatment of stable angina pectoris has high safety.

Objective:To investigate the expressions of tumor stem cell marker CD133 and tumor vascular endothelial cell marker CD105 in lung cancer tissue and their clinical significance.Methods:Streptavidin-biotin-peroxidase complex (SABC)-immunohistochemical staining was used to detect the expression of CD133 and CD105 in 65 cases of lung cancer tissue and 30 specimens of adjacent non-cancerous tissue. The relationship between the expression of CD133/CD105 and tumor size, histological types, differentiation, TNM stage, lymphoid metastasis, and prognosis were analyzed. Results:The positive expression rates of CD133 and CD105 in lung cancer tissues were significantly higher than those in adjacent non cancerous tissues, respectively (69.2% vs 26.7% and 67.7% vs 10.0%, both P<0.05). The positive expression rates of CD133 and CD105 in the group with lymphoid metastasis were higher than those with non lymphoid metastasis, respectively (82.5% vs 48.0% and 80.0% vs 48.0%, both P<0.05). The expressions of CD133 and CD105 were positively correlated with lymphoid metastasis with Pearson coefficient r of 0.35 and 0.32, respectively (P<0.05). No significant correlation was found between the expression of CD133/CD105 and tumor size, histological types, differentiation degree as well as TNM stage(P>0.05). The expression of CD133 was positively correlated with the expression of CD105 in lung cancer tissue with Pearson coefficient r of 0.37 (P<0.05). Postoperative median survival periods of CD133 and CD105-positive group were significantly shorter than the CD133 and CD105-negative groups (37 months vs 66 months,35 months vs 70 months, respectively, both P<0.05). Conclusion:The expression of CD133 and CD105 was associated with lymphoid metastasis and prognosis in patients with lung cancer.Their overexpression implies poor prognosis of lung cancer patients.

Objective To find out some ways to solve the problems which are dealing with the clinical application of intrafix.Methods From February to May 2013,324 patients with back blood using intrafix in emergency department were randomly assigned to the experimental group A and the control group A,with 162 patients in each group.The experimental group A used the method of pressing pipe while pulling out the needles when transfusion was over,while the control group A used traditional ways.206 patients who occurred bubbles in the intrafix among the above two groups were divided into the experimental group B and the control group B.The experimental group B got rid of the bubbles using top-down method,the control group B used traditional ways.The effect of withdrawing the needles and exhaustion of bubbles were compared.The least flushing time of remaining liquid using transfusion pipe flushing method was calculated.Results The experimental group A and the experimental group B were better in withdrawing the needles and exhaustion of bubbles compared with those of the control group A and the control group B.Using the method of drop coefficient to calculate the least flushing time can ensure the drug to be transfused into the body thoroughly.Conclusions When transfusion is over,pressing intrafix pipe will have a better result than the traditional way when pulling out the pipe.Top-down method can clearly settle the problem of intrafix pipe,which can not get rid of bubble via traditional way.Flushing pipe and press pipe method can apparently decrease drug waste.

BACKGROUND:Chronic hepatitis B virus infection can impact the biological characteristics of bone marrow mesenchymal stem cel s. Adipose-derived mesenchymal stem cel s have gained more and more attention due to their high safety, little invasiveness, easy purification and rapid proliferation. OBJECTIVE:To establish the isolation and culture method of adipose-derived mesenchymal stem cel s from patients with hepatitis B virus infection in vitro, and to observe the biological characteristics of cel s. METHODS:Adipose-derived mesenchymal stem cel s were isolated from the subcutaneous fat of hepatitis B virus infection patients by col agenase digestion and adherent method. Growth curve of adipose-derived mesenchymal stem cel s were detected by MTT method and cel phenotypes were detected by flow cytometry. The adipogenic and osteogenic differentiation potential of adipose-derived mesenchymal stem cel s were detected in vitro. RESULTS AND CONCLUSION:(1) Adipose-derived mesenchymal stem cel s from 10 patients with hepatitis B virus infection were al isolated and cultured successful y. The primary passage time of adipose-derived mesenchymal stem cel s was (8.3±1.2) days. The growth curve of cel s was“S”shaped. Cel s came into a logarithmic phase at days 3-4, and came into platform at day 7. (3) Passage 3 adipose-derived mesenchymal stem cel s highly expressed CD29, CD166, HLA-ABC and CD44, but did not express or lowly expressed CD34 and HLA-DR. (3) Adipose-derived mesenchymal stem cel s differentiated into adipocytes after adipogenic induction, and differentiated cel s were positive for oil red O staining;after osteogenic induction, adipose-derived mesenchymal stem cel s differentiated into osteoblasts that were positive for alkaline phosphatase staining. These findings indicate that the col agenase digestion and adherent method can be used to effectively isolate adipose-derived mesenchymal stem cel s from patients with hepatitis B virus infection, and the cel proliferation is rapid so that a large number of cel s can be obtained in the short term.

Myelodysplastic syndromes (MDS) is a heterogeneous group of clone bone marrow disorders.The diagnosis of MDS can be challenging when dysplastic features are minimal and cytogenetic analysis shows no abnormalities The high sensitivity of flow cytometry (FCM) in the detection of dysplasia in myeloid lineages makes it a valuable tool to distinguish possible clonal causes of cytopenia(s) from nonclonal causes.Due to the heterogeneity and absence of specific FCM markers for MDS,the immunophenotyping of MDS by FCM remains a big difficulty.Here we will focus on how to perform immunophenotyping of MDS by FCM and the potential diagnostic,prognostic and therapeutic value of FCM in MDS.

Objective To explore the effect of spironolactone on vascular remodeling of injuried rabbit artery. Methods 20 male New Zealand rabbits were randomly divided into two groups. The rabbit models of iliac artery angioplasty were set up by BC. After injury, control group was fed with standard forage, and experimental group was fed with standard forage and spironolactone(20mg?kg~ -1?d~ -1). The rabbits were killed four weeks after injury. The injuried segments of iliac artery were removed, and processed for histological and morphological analysis by imumohistochemistry and TD 2000 analysis system of pathology image. Results AUIEM and AUEEM in the experimental group significantly increased as compared with the control group(P0.05). Conclusion Spironolactone could improve vascular remodeling after angioploasty of iliac artery in rabbits.

Objective To observe the analgesia effects of nitrous oxide inhalation in childbearing. Method Respectively estimate the labor time,delivery type,degrees of labor pain, fetal distress and postpartum hemorrhage condition between analgesia group of 86 cases and control group of 45 cases. Result Compared to control group, the painful extent of delivery process in analgesia group was obvious lightened and childbearing time was decreased. Conclusion BZ Analgesia effects of nitrous oxide inhalation is positive in childbearing and has great value of clinical applying in obstetrics.

Twenty-two neurosurgical patients were randomly divided into two groups:Group 1 iv drip midazolam and isoflurane inhalation;Group 2 isoflurane inhalation only. The hemodynamic indices and concentration of isoflurane were studied. The results showed that the blood pressure of all the patients were significantly decreased(PMO.01). The concentration of isoflurane in group 1 were .signicantly lowered by 27% than in group 2(P