OBJECTIVE: To study the sedative and antistress action of Shenqiwuweizi capsule. METHODS: The sedative and hypnotic experiments were made to observe the action of Shenqiwuweizi capsule on the mice's sleep induced by pentobarbital sodium at subthreshold dosage or hypnotic dosage. The swimming and hypoxia tolerance experiment were performed to observe the effects of Shenqiwuweizi capsule on the swimming time and hypoxia tolerance time in mice. RESULTS: Shenqiwuweizi capsule could obviously prolong the sleeping time of mice induced by pentobarbital sodium, increase the number of sleeping animals caused by pentobarbital sodium at subthreshold dosage, and significantly prolong the time of swimming and hypoxia tolerance in mice. CONCLUSION: Shenqiwuweizi capsule had sedative and anti-stress action.

To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.

Currently,although surgical aortic valve replacement (SAVR) is still the golden standard in treatment of severe aortic stenosis according to the guideline,transcatheter aortic valve implantation (TAVI) is gradually becoming a common treatment for patients who are prohibitive or in high risk for SAVR.Recently,the valve manufacturers,including medical companies in China,are making their utmost to develop valve device,leading remarkable results achieved by TAVI.With the complications being controlled,TAVI displays promising future.It is likely that TAVI is expected to become a substitute for SAVR to treat patients with aortic stenosis or even aortic regurgitation.

OBJECTIVETo compare and classify the samples of Angelica sinensis from 36 different areas in Gansu province.

METHODThe HPLC was used to detect samples, and the computer aided similarity evaluation was used to analyze the fingerprints. Similarity combined with principal component analysis (PCA) and multidimensional pattern recognition of Euclidean distance were used to compare and classify the samples of A. sinensis in different areas.

RESULTIt was found that the quality of A. sinensis is closely related to the growth environment.

CONCLUSIONThis method could be used to classify the samples of A. sinensis from a variety of sources.

Objective To investigate whether chlorophyllin could protect human umbilical vein endothelial cell (HUVEC) against oxidative damage by inducing the expression of heme oxygenase-1 (HO-1) and to explore the underlying mechanism.Methods The cellular protection of chlorophyllin against oxidative damage was detected by cell-survival assay with flow cytometry.The level of free radicals was detected directly by electron spin resonance spectra.The induced expression of HO-1 was shown by RT-PCR,Western blot,immunofluorescence confocal laser microscopy and enzymatic activity test.Whether the activation of PI3K/Akt pathway was involved was detected by Western blot.Results Chlorophyllin could protect HUVEC against oxidative damage caused by H2O2 via scavenging the excessive free radicals.Chlorophyllin treatment could induce expression of HO-1 in a dose- and time-dependent manner.The activation of PI3K/Akt pathway was required in the induction of HO-1.LY294002,the specific inhibitor of PI3K,could suppress the activation of PI3K/Akt and the induced expression of HO-1 in a dose-dependent manner.Conclusions Chlorophyllin shows cellular protection against oxidative damage by counteracting the excessive free radicals.Up-regulation of HO-1 expression plays a pivotal role in the protection of chlorophyllin,while the activation of PI3K/Akt signaling pathway is required in the induction of HO-1.

Allergic bronchial pulmonary aspergillosis is a kind of lung disease that is a body of the parasitic in the bronchial aspergillus caused by allergic reactions.This disease usually occurs in patients with asthma and cystic fibrosis.Due to China's high incidence of asthma,to improve the awareness of asthma in patients with allergic bronchopulmonary aspergillosis and timely treatment are particularly important.Glucocorticoid inhibition of immune inflammatory response combined with antifungal agents to reduce fungal load is the main treatment.There are also anti-IgE treatment and other treatment.

Intracerebral hemorrhage is a kind of nervous system disease with high mortality and disability. By analyzing the clinical variables closely associated with the functional outcome of intracerebral hemorrhage, a scoring scale that can quickly predict the outcome of intracerebral hemorrhage is established, which is helpful to guide clinicians in risk stratification and clinical diagnosis and treatment of patients with intracerebral hemorrhage. At present, more than 10 clinical outcome scoring scales have been developed. This article summarizes these scoring scales of intracerebral hemorrhage in chronological order, and reviews their constituent variables, outcome evaluation value, and follow-up verification.

Objective To investigate whether autophagy can be induced by 1,4-benzoquinone (1,4BQ) in HL60 cells,as well as the role of reactive oxygen species (ROS)in induced autophagy.Methods In order to determine a suitable 1,4-BQ treatment concentration for autophagy detection in HL60 cells,the cell vitality were examined by CCK8 assay.Logarithmic-growth-phased cells were divided into control group,1,4-BQgroup(10μmol/L 1,4-BQ,24 h),NAC group (antioxidant n-acetyl cysteine,5mmol/L,24 h) and the 1,4-BQ+NAC group (5 mmol/L NAC were preincubated for 1h prior to the treatment with 10 μmol/L 1,4-BQ for 24 h).The autophagic acidic vesicle were inspected by acridine orange staining,LC3 were detected by immunofluorescence staining,and expressions of LC3 and Beclin1 were quantitatively detected by Western blot.Results The results from cell viability test indicated that 1,4-BQ exhibited a dose-dependent toxicity to HL60cells.Compared with control group.the cell viability in 20.0、40.0μmol/L concentration were decreased obviously,and the differences had statistical significance (P＜0.05).Compare with contrd group acidic vesicle,LC3II,LC3II/LC3I and Beclin1 protein expressions were increased in 1,4-BQ group,after both respectively 12.4％ and 27％,the differences had statistital significance.While 1,4-BQ+NAC group was observed that acidic vesicle,LC3 and Beclin1 protein level were markedly lower than 1,4-BQ group,after both decreased 12.6％ and 22.6％ respectively,both the difference were statistically significant (P＜0.05).Conclusion 1,4-BQ can induce autophagy in HL60 cells,the induction of autophagy is at least partly resulted from ROS.Antioxidant can effectively suppress the occurrence of induced autophagy.

Objective To investigate whether autophagy can be induced by 1,4-benzoquinone (1,4BQ) in HL60 cells,as well as the role of reactive oxygen species (ROS)in induced autophagy.Methods In order to determine a suitable 1,4-BQ treatment concentration for autophagy detection in HL60 cells,the cell vitality were examined by CCK8 assay.Logarithmic-growth-phased cells were divided into control group,1,4-BQgroup(10μmol/L 1,4-BQ,24 h),NAC group (antioxidant n-acetyl cysteine,5mmol/L,24 h) and the 1,4-BQ+NAC group (5 mmol/L NAC were preincubated for 1h prior to the treatment with 10 μmol/L 1,4-BQ for 24 h).The autophagic acidic vesicle were inspected by acridine orange staining,LC3 were detected by immunofluorescence staining,and expressions of LC3 and Beclin1 were quantitatively detected by Western blot.Results The results from cell viability test indicated that 1,4-BQ exhibited a dose-dependent toxicity to HL60cells.Compared with control group.the cell viability in 20.0、40.0μmol/L concentration were decreased obviously,and the differences had statistical significance (P＜0.05).Compare with contrd group acidic vesicle,LC3II,LC3II/LC3I and Beclin1 protein expressions were increased in 1,4-BQ group,after both respectively 12.4％ and 27％,the differences had statistital significance.While 1,4-BQ+NAC group was observed that acidic vesicle,LC3 and Beclin1 protein level were markedly lower than 1,4-BQ group,after both decreased 12.6％ and 22.6％ respectively,both the difference were statistically significant (P＜0.05).Conclusion 1,4-BQ can induce autophagy in HL60 cells,the induction of autophagy is at least partly resulted from ROS.Antioxidant can effectively suppress the occurrence of induced autophagy.

Objective To investigate the role of RNA-binding motif protein X-linked(RBMX)in regulating the proliferation,migration,invasion and glycolysis in human bladder cancer cells.Methods A lentivirus vectors system and RNA interference technique were used to construct bladder cancer 1376 and UC-3 cell models with RBMX overexpression and knockdown,respectively,and successful cell modeling was verified using RT-qPCR and Western blotting.Proliferation and colony forming ability of the cells were evaluated using EdU assay and colony-forming assay,and cell migration and invasion abilities were determined using Transwell experiment.The expressions of glycolysis-related proteins M1 pyruvate kinase(PKM1)and M2 pyruvate kinase(PKM2)were detected using Western blotting.The effects of RBMX overexpression and knockdown on glycolysis in the bladder cancer cells were assessed using glucose and lactic acid detection kits.Results RT-qPCR and Western blotting confirmed successful construction of 1376 and UC-3 cell models with RBMX overexpression and knockdown.RBMX overexpression significantly inhibited the proliferation,clone formation,migration and invasion of bladder cancer cells,while RBMX knockdown produced the opposite effects.Western blotting results showed that RBMX overexpression increased the expression of PKM1 and decreased the expression of PKM2,while RBMX knockdown produced the opposite effects.Glucose consumption and lactate production levels were significantly lowered in the cells with RBMX overexpression(P<0.05)but increased significantly following RBMX knockdown(P<0.05).Conclusion RBMX overexpression inhibits bladder cancer progression and lowers glycolysis level in bladder cancer cells by downregulating PKM2 expression,suggesting the potential of RBMX as a molecular target for diagnosis and treatment of bladder cancer.