It is important to protect the subjects'right of informed consent in biomedicine research.In this study,we focused on discussing how to protect the subjects' right of informed consent,including the perspectives of leg principles,the obligations of the researchers,the functions of Institutional Review Board,and the self-protection of subjects.

In the process of scientific research management informationization,some critical questions are proposed.It is necessary to investigate comprehensively the information construction,and make a plan with overall point of view.Meanwhile,it is of importance to think highly of the standardization and practicability of information,keep good coordination and communication with all persons involved in the information construction,maintain and renew the information duly,draw the assistance from foreign technical support,and utilize the achievements of the informationization to improve the management.

Objective To express and purify Cc PT1 protein from Aspongopus chinensis in prokaryotic cell.Methods Thesynthesized Cc PT1 gene was cloned to vector p GEX-4T-1 to construct recombinant expression plasmid p GEX-4T1-Cc PT1,which was then transformed to competent E.coli Rosetta strain and induced by IPTG.The induction temperature(20 ℃ and37 ℃),final concentration of IPTG(0.25,0.5,0.75 and 1 mmol/L)and induction time(6,8,10,12 h)were opti-mized.The obtained protein was purified by GST protein purification system,which was then analyzed by 10% SDS-PAGEand identified by Western blot.GST tags were removed by Pre Scission Protease during purification.Results The recombi-nant protein GST-Cc PT1 was expressed in the form of inclusion body with a concentration of 0.026 9 mg/ml,of which therelative molecular mass was 29 800,consistent with the expectation.The optimum induction condition was induction withIPTG of final concentration of 0.75 mol/L for 12 h at 20 ℃.The purified protein was more than 90% in purity and boundspecifically to mouse monoclonal antibody against GST.After remove of GST tags,Cc PT1 protein showed a relative molecu-lar mass of about 2 830 and the yield was 11.15%.Conclusion A.chinensis Cc PT1 protein was expressed by prokaryoticexpression system,and the purity of Cc PT1 protein was high after purification,which laid a foundation of the in-depth studyof anticancer peptides of A.chinensis.

Objective　To analyze the current epidemiological situation of measles and to put forward strategies for controlling measles in Xinjiang. Methods　A descriptive epidemiology was performed to survey incidence of measles in Xinjiang from 1953～1999. Results 　The highest incidence rate of 1 155.14 per 100 000 was reported in 1964. Since 1 965, the measles incidence had dramatically decreased with using measles vaccine in Xinjiang, particularly the action of launching EPI program since 1984. The l owest incidence rate of 7.85 per 100 000 was achieved in 1990. From 1990～1 999 cases mostly concentrated on preschool and school children. Seasonal distri b ution of measles was in April and May. Conclusions　It is urgent to improve efficacy of measles vaccine, to establish measles epidemiological an d laboratory surveillance system, and to strengthen immunization for measles and controlling of the outbreak.

Objective:To summarize the clinicopathologia and imaging features of Hemiballism-hemichorea induced by hyperglycemia and increase the understanding of the disease and improve the accuracy of diagnosis. Methods:The imaging images and clinical manifestation of 5 cases Hemiballism-hemichorea induced by hyperglycemia were analyzed retrospectively. The disease pathogenesis and mechanism relating to the Characteristic neuroradiological findings was obtained by literature review. Results:The common clinical manifestation of Hemiballism-hemichorea induced by hyperglycemia is unilateral limb involuntary movements. CT scans typically show an area of hyperdensity in the basal ganglia and The characteristic finding on the T1-weighted MRI is high signal intensity in the contralateral putamen. Conclusion: Hemiballism-hemichorea induced by hyperglycemia is an unusual but important differential diagnosis in patients with particular neuroradiological findings as prompt diagnosis and treatment of hyperglycemia has an excellent prognosis. The correct diagnosis could be received through integrated the patient's past medical history, presentation, laboratory and imaging inspection into account.

Objective To investigate the application value of low kilovolt technique combined with lower contrast in the head and neck angiography by the second-generation dual source CT.Methods From October 2013 to January 2015,120 patients undergoing head and neck computed tomography angiography (CTA) were randomly divided into groups A,B,C,D.Each group was given a different tube current and different dose of contrast agent and saline solution 50 ml.Group A received current 100 mA and contrast agent 50 ml,group B received current 100 mA and contrast agent 40 ml,group C received current 80 mA and contrast agent 50 ml,and group D received current 80 mA and contrast agent 40 ml.CT values of aortic arch,bilateral common carotid artery bifurcation,double sided M1 segment of middle cerebral artery,basilar artery enhanced degree of the straight sinus were measured in each group.Residual artifacts caused by contrast agent in brachiocephalic vein and subclavian vein were observed.5-score method was used to assess the quality of reconstructed image,and the radiation exposure dose was calculated.Results The mean effective dose was reduced by 29％ in group C as compared with group A [(0.53±0.17) mGy vs.(0.74±0.04) mGy].There was no significant difference in developing strength in cerebral arteries angiography between group C and group A (P=0.247),but the inage noise was slightly larger in group C than group A,without significant differences (P=0.203).The average effective dose in group A was almost the same as that in group B [(0.74 ± 0.04) mGy vs.(0.73 ± 0.05) mGy].Structure display of cerebral arteries on CT volume rendering (VR) and multiplane reformation (MPR) images had no significant differences between group A and group B (P=0.114).The average effective dose in group C was almost the same as that in group D[(0.53 ± 0.17) mGy vs.(0.53 ±0.01) mGy].Structure display of cerebral arteries on CT volume rendering (VR) and muhiplane reformation (MPR) images had no significant differences between group C and group D (P=0.109).The mean effective dose was reduced by 28％ in group D as compared with group B[(0.53±0.01)mGy vs.(0.73±0.05) mGy].There was no significant difference in developing strength in cerebral arteries angiography between group C and group A (P=0.236),but the image noise was sligbtly larger in group C than group A (P =0.212).Conclusions Application of low tube current combined with low concentration of contrast agent in the head and neck dual-source CT angiography is feasible in clinical diagnosis,with good clinical value.It can not only get better image quality,but also meet the needs of clinical diagnosis.

Chemotherapy is one of the primary treatments for cancer patients,while tumor multidrug re-sistance( MDR) represents a major obstacle in the treatments of patients,limiting the efficacy of chemotherapy.To date,the mechanism of drug resistance has not been fully elucidated.lncRNAs are a family of long noncoding RNAs that regulate gene expression by epigenetic modification,transcription and post transcriptional processing. Recent studies have showed that the aberrant regulation of target genes by lncRNAs may be involved in the acqui-sition of tumor MDR.Therefore,targeting lncRNAs may be an attractive strategy for improving the chemosentivity and reversing chemotherapeutic resistance.This paper reviews the relationship between lncRNAs and tumor drug resistance as well as the underlying possible mechanism.

The etiology, pathogenesis, and diagnostic criteria of chronic prostatitis were reviewed in this article. Based on clinical practice, the authors systematically discussed the thoughts and methods for the treatment of chronic prostatitis by integrated traditional Chinese and Western medicine. Meanwhile, advice on disputed problems in clinical study of prostatits were given, such as curative effect estimation value of the number of leukocytes in expressed prostatic secretion (EPS) and bacterial culture in EPS, the opportunity and treatment course of antibiotics, National Institutes of Health chronic prostatitis symptom index, classification of syndromes of traditional Chinese medicine (TCM), TCM symptom score, and clinical study period.

This paper discusses project management of translational medicine in several important aspects:scientific planning,platform constructing,team building,process management,technology transfer,protection of intellectual property rights,supervision on ethics,and project funding system of the institutions.

Objective To investigate the best pathologically diagnostic method for breast cancer before surgery. Methods Wet fine needle aspiration cytology was conducted in 68 cases of probable breast cancer by using a 10 ml syringe with size 7 needle. Results Malignant cells were found in 19 cases,without any false positive cases.Of the remaining 49 cases of negative cytological findings: 46 cases underwent segmental mastectomy,1 of which was postoperatively diagnosed as having fibrosarcoma by paraffin section examination and the rest of which were all demonstrated the presence of benign tumors;3 cases without surgical intervention were followed for (11～20) months and no evidences of breast cancer were found.The sensitivity was 95 0% (19/20),the specificity was 100% (48/48),the accuracy rate was 98 5% (67/68),and the false negative rate and false positive rate were 5 0% (1/20) and 0 (0/48),respectively.Of the 19 cases of positive cytological findings,no implantation metastasis in needle passage occurred.There were no complications in the study. Conclusions Wet fine needle aspiration cytology is safe,economical,convenient,quick,almost painless,and accurate,being the method of first choice in the diagnosis of breast cancer.