OBJECTIVE To investigate genes associated with the drug-resistance of ?-lactamases antibiotics in Pseudomonas aeruginosa(PAE) and type Ⅰ integrating enzyme gene isolated from clinical patients.METHODS The drug-sensitivity for 16 antibiotics were detected by the disc agar diffusion(DAD) and microdilution.To ditect the related drug-resistence genes TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,F-OX,MOX,CTX-M9,CTX-M1 and outer membrane protein gene oprD2 and 18 main drug resistance genes of typeⅠ integrating enzyme gene and others with PCR method.RESULTS Resistant rates of 34 multidrug resistant P.aeruginosa(MRPA)strains for amikacin,ceftazidime,ciprofloxacin,cefoperazone/sulbactam,cefepime,imipenem,levofloxacin,piperacillin/tazobactam,aztreonam,piperacillin and tobramicin were 14.7%,29.4%,61.8%,67.6%,70.6%,73.5%,76.5%,76.5%,79.4%,91.2% and 94.1%,respectively,with 100.0% for others antibiotics.The positive diagnosis rates of gene encoding extended-spectrum ?-lactamases FOX,IMP,DHA,and VIM were 23.5%,11.8%,11.8% and 5.9%,24(70.6%) were deleted in outer membrane protein gene oprD2,other ?-lactamase genes were absent in all isolates,the type Ⅰ integrating enzyme gene intⅠ1 was positive in 34 strains.CONCLUSIONS P.aeruginosa carries various ?-lactamases genes in clinical PAE patients,and the deletion ratio of oprD2 gene is high,type Ⅰ integrating enzyme exists largely in MRPA.

Background and purpose:Vascular endothelial growth factor-C(VEGF-C) is ranked first as a lymphoangiogenic factor, which induces lymphatic proliferation and spread of solid tumors. Lymph node status remains the strongest prognostic factor in a variety of human malignant tumors including uterine cervical carcinomas and lymphnode metastasis is stimulated by tumor cell aggressiveness, such as abilities of growth and metastases.We examined the correlation between VEGF-C expression and tumor aggressiveness in cervical carcinomas with respect to clinicopathologic features and patient outcome and to see whether the expression of VEGF-C and its receptor in cervical carcinoma tissue and their paratumour tissue had a role in tumor metastasis and other clinical significance. Methods:48 fresh cervical cancer tissues and their paratumour tissue were examined by RT-PCR to detect the VEGF-C/KDR/Flt-4mRNA expression.Results:There was a significant difference of VEGF-C/KDR/Flt-4mRNA expression between the tumor , paratumour tissue and the normal controls. And there was no significant relationship between VEGF-C mRNA, KDR mRNA or flt-4mRNA expression with the pathological types and clinical stage of invasive carcinoma of cervix. However,expression was significantly associated with the grade of tumor pathology, lymph node metastasis, tumor size and the invasion of deep muscular layer(P

OBJECTIVE To analyze the multi-drug resistant genes(genes encoding extended-spectrum ?-lactamases,outer membrane protein gene oprD2,aminoglycoside-modifying enzyme genes,sulfonamides and disinfectant-resistance gene and plasmid-mediated quinolone resistance gene) of Pseudomonas aeruginosa clinical strains.METHODS The drug-resistance to 15 antibiotics was detected by the disc agar diffusion(DAD) and microdilution broth method in 32 P.aeruginosa strains isolated from Jun to Dec 2006.Twenty-eight related drug-resistant genes and outer membrane protein gene oprD2 were examined by PCR method.RESULTS The resistance rate of 32 P.aeruginosa strains to amikacin,ceftazidime,ciprofloxacin,cefepime,cefoperazone/sulbactam,imipenem,meropenem,levofloxacin,piperacillin/tazobactam,aztreonam and piperacillin were 9.4%,25%,59.4%,68.7%,68.8%,78.1%,81.1%,81.3%,84.4% and 94.4%,respectively,and that to others antibiotics were 100%.The detective rate of aminoglycoside-modifying enzyme genes(aac(6′)-Ⅱ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aph(3′)-Ⅵ,ant(3″)-Ⅰ and aac(3)-Ⅰ) were 68.8%,62.5%,21.9%,9.4%,9.4% and 6.3%,and the genes encoding extended-spectrum ?-lactamases(blaFOX,blaIMP,blaVIM and blaDHA) were 37.5% 15.6% 6.3% and 9.3%,respectively.Twenty-two(68.8%) of them were detected in oprD2 and 9(28.1%),but positive in qacE?-sul1 gene.CONCLUSIONS The detective rate of aminoglycoside-modifying enzyme genes,extended-spectrum ?-lactamases genes and the loss rate of outer membrane protein gene oprD2 are high in these 32 multi-drug resistant P.aeruginosa clinical strains.

OBJECTIVE To investigate genes associated with aminoglycosides modification enzymes(AMEs)in Pseudomonas aeruginosa(PAE)isolated from NICU patients.METHODS Drug-resistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(3)-Ⅲ、aac(3)-Ⅳ,aac(6')-Ⅰ,aac(6')-Ⅱ,aph(3')-Ⅵ,ant(3″)-Ⅰand ant(2″)-Ⅰwere detected bypoly merase chain reaction(PCR)amplification in 36 PAE isolates.The bacteria were identified by ATB.RESULTS The positive rates of aac(6')-Ⅱ,aac(3)-Ⅱ,aac(6')-Ⅰ and aph(3')-Ⅵ genes were 52.8%,47.2%,11.1% and 2.8% of 36 isolates,respectively.Drug-resistant genes encoding AMEs were detected positively in 77.8% of 36 isolates.CONCLUSIONS AMEs genes are present in high percentage of PAE isolated from NICU patients.

Background and Purpose:Cervical cancer is one of the most common malignant neoplasms among women.The 5-year survival for patients with early stage disease is over 70%.While it is generally accepted that either radical surgery or radiotherapy can achieve cure for the majority of patients with early-stage cervical cancer,there is no standard approach to the patients with bulky disease,whose prognosis remains very poor in spite of the therapeutic advances achieved in recent years.Neoadjuvant chemotherapy has been used as part of the multidisciplinary treatment before either operation or radiation therapy.Although the approach still had many controversies,numerous studies have supported its effectiveness in bulky cervical cancer.The present study was conducted to explore whether neoadjuvant chemotherapy with the combination of vincristine,bleomycin and cisplatin could improve the operability and pathological response rate in Ⅰb_(2)~Ⅱb bulky cervical cancer patients.Methods:A total of fifty-six patients with bulky cervical cancer were histologically diagnosed and staged into Ⅰb_(2)~Ⅱb according to the standards of International Federation of Gynecology and Obstetrics(FIGO) and randomly selected,including 15 cases with Ⅰb_(2),15 with Ⅱa,26 with Ⅱb.They were treated by a regimen of PVB chemotherapy for 2 cycles and followed by operation.The immediate response and adverse effects were analyzed.Results:The overall response rate was 78.6%.The resectability rate for surgery after chemotherapy was 100%.No intolerable toxic and adverse effects were recorded during neoadjuvant chemotherapy.Preoperative neoadjuvant chemotherapy can apparently increase the possibility of resection by operation and enhance the remission rate.The primary tumor sizes were significantly decreased after chemotherapy before surgery(P

Objective To analyze the changing process and development trend of deaths (mortality)in patients with viral hepatitis after the use of the Internet-based National Notifiable Infectious Disease Reporting System.Methods Descriptive statistical and moving average methods were employed to analyze the infectious disease reports released by the Chinese government during 1992-2013,and the changing process and development trend of deaths (mortality)in patients with viral hepatitis in China over the past 10 years were investigated.Re-sults The annual deaths (mortality)in patients with viral hepatitis showed a decreasing trend.The deaths (mortality)from hepatitis B showed a slight declining trend,accounting for a great proportion (over 70%)of the deaths (mortality)from viral hepatitis;the deaths (mortality)from hepatitis C had shown a slight downward trend since 2007;the deaths (mortality)from hepatitis A and unclassified hepati-tis decreased to different extents;the deaths (mortality)from hepatitis E remained at a relatively low level.Conclusion The treatment of viral hepatitis has been gradually improved in China and the public awareness of viral hepatitis keeps increasing.However,much attention still needs to be given to the prevention and treatment of viral hepatitis,especially hepatitis B and C,by the government.

Objective:To examine the expression of VEGF gene in the endometrial carcinoma tissues,para-tumor tissues,normal endometria and peripheral blood,and analyze the role of VEGF in tumor growth and tumor metastasis.Methods:Real-time fluorescence quantitative PCR was used to detect the expression of VEGF gene in 51 endometrial carcinoma samples and the corresponding para-tumor tissues,40 normal endometria samples and their corresponding peripheral blood samples.The relation between the VEGF expression and clinical pathological parameters was analyzed.Results:The expression of VEGF gene was higher in the endometrial carcinoma tissues than in the corresponding para-tumour tissues and normal endometrial tissues(P0.05).The expression of VEGF in peripheral blood was higher in patients with endometrial carcinoma than that in the normal controls;and the expression was significantly correlated with the clinical stage,histological grades,pathological types and the presence of lymph node metastasis(P0.05).Conclusion:Real-time fluorescent quantitative PCR can sensitively,specifically detect the expression of VEGF in the endometrial carcinoma tissues and peripheral blood samples.VEGF might play an important role in the development,invasion,and metastasis of endometrial carcinoma.

Objective: To explore the relationship between the combinative intervention therapy and clinical curative effect, through used function magnetic resonance imaging (fMRI) to observe the change of detection data for patients with cerebral infarction pre and post-intervention therapy of acupuncture combined with traditional Chinese medicine (TCM). Methods: 40 patients with cerebral infarction were divided into control group (20cases, received normal treatment)and observation group (20cases, received combinative treatment of acupuncture and TCM). Pre and post therapy, the patients of every group received evaluation about state of illness and detection of fMRI. The score of national institute of health stroke scale (NIHSS) was used to evaluate the degree of neurologic impairment, and analyze the imaging manifestations of fMRI on patients with cerebral infarction at basal ganglia who received acupuncture combined with TCM. Some cerebral functional imaging techniques, included blood oxygenation level dependent( BOLD), fMRI and diffusion tensor imaging (DTI), were used to grasp the changes of relevant functional region of brain and different brain regions connected with function region of patients with cerebral infarction before and after therapy. Results: The NIHSS score of observation group was significantly less than that of the control group (t=5.450, P<0.05), and the total efficiency of observation group (95%) was significantly higher than that of the control group (75%) (x2=4.329, P<0.05). And the differences of the activation frequency, activation volume, ADCavg values and the values of FA between the observation group and the control group were statistically significant (t=5.425, 2.760, 3.453, 5.513, P<0.05). Conclusion: Acupuncture combined with traditional Chinese medicine can efficiently improve the status of neurologic impairment for patients with cerebral infarction, and the clinical efficacy before and after treatment can preferably be observed by fMRI.

Objective: to select the optimal acupuncture therapeutic projects on cerebral infarction-related motor dysfunction of lower limbs. Methods: to optimize the combination projects on 4 factors and 3 levels affecting the acupuncture effect on cerebral infarction by using orthogonal design targeting on patients with cerebral infarction-related motor dysfunction of lower limbs, and Fugl-Meyer score of limb motor function was taken as indexes. Results: The relatively optimal Fugl-Meyer score of lower limb function can be obtained within 3-day duration of cerebral infarction. Conclusions: As far as the considered factors and levels are concerned, the previously mentioned project is the optimal acupuncture therapeutic project for cerebral infarction-related motor dysfunction of lower limbs.

OBJECTIVE To investigate and analyze the application of prophylactic antibiotics in perioperation period in our hospital.METHODS According to the designed table with unified contents and standards,a retrospective investigation was made in 325 patients who had been discharged from our hospital.RESULTS Of the 325 patients in our study,antimicrobials use in 281 patients was rational,44 patients had irrational antimicrobials use.CONCLUSIONS The results show that it is very important to strengthen and improve the management of the antimicrobials use in a normal and standard way in our hospital.