1.Application of protective lung ventilation model in thoracic surgery patients with single lung ventilation
Journal of Chinese Physician 2018;20(6):886-888,892
Objective To explore the value of protective lung ventilation mode in patients undergoing single lung ventilation in thoracic surgery operation.Methods 59 patients undergoing surgical treatment in Ankang Central Hospital from January 2012 to January 2015 were analyzed.According to the ventilation mode adopted in the operation,the patients were divided into the protective group (30 cases) and conventional group (29 cases).The protective group took tidal volume 8 ml/kg.According to the patient's circulatory function,we gradually increase the end expiratory positive pressure ventilation,until 10 cmH2O.The conventional group took tidal volume 8 ml/kg and was maintained positive end expiratory pressure at 3 cmH2O.Results There was no statistically significant difference in operation time,blood volume,infusion volume,and single lung ventilation time between the protective group and the conventional group (P > 0.05).There was also no statistically significant difference in T1,T2,T3,T4,inhalation of oxygen partial pressure (FiO2),mean arterial pressure (MAP) level between the two groups (P > 0.05).The T2 and T3 moment of arterial oxygen partial pressure (PaO2) oxygenation index (OI) level in protective group was significantly higher than that in conventional group (P < 0.05).The preoperative serum tumor necrosis factor-α (TNF-α),interleukin-8 (IL-8),superoxide dismutase (SOD),macrophage inflammatory protein Ⅰ (MIP-Ⅰ),malondialdehyde (MDA) level between the two group showed no significant difference (P >0.05).While after the operation,the serum TNF-alpha,MIP-Ⅰ,IL-8 and the level of MDA in protective group was significantly lower than that of conventional group (P < 0.05),the SOD level showed the opposite (P < 0.05).Conclusions The protective lung ventilation mode is beneficial to maintain the blood gas indexes,at the same time reduce the inflammatory response and protect the lung function of the patients.
2.Research on Registration System of Medical Device Master Files in China.
Peng ZHAO ; Shupei ZHANG ; Xinli SHI
Chinese Journal of Medical Instrumentation 2021;45(2):205-209
The registration system of medical device Master Files is established to solve the problem that the outsourcing suppliers are not willing to cooperate with the device applicants in the process of providing medical device application documents. After a brief introduction of Master Files systems established by foreign regulatory agencies, this article focuses on the research of establishing a medical device Master Files registration system in China. The results show that the establishment of Chinese Master Files registration system can both improve the standardization and convenience of outsourcing activities of medical devices, and satisfy the needs of the development of medical device industry and regulatory system. At the same time, the probability of additional risk caused by the implementation of the system is low. Therefore, it is expected that the benefits of the system to promote public health outweigh the potential risks, which demonstrates that establishment of the system has important application values.
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3.Effect of remote limb isehemic postconditioning on posterior circulation ischemia vertigo
Huaiguo WU ; Qingbing HOU ; Shupei WANG ; Yanliu JIANG ; Lu ZHANG
Chinese Journal of Neuromedicine 2016;15(7):660-663
Objective To study the effect of remote limb isehemic postconditioning (RLIPC) on posterior circulation ischemia vertigo (PCIV). Methods Seventy patients with PCIV, admitted to our hospital from January 2013 and June 2014, were randomly divided into control group and therapy group (n=35). The patients of control group were treated with routine medicine, and those of therapy group were treated with RLIPC and routine medicine. Before and after treatment, dizziness assessment rating scale (DARS) and dizziness handicap inventory (DHI) scale were used to evaluate the changes of manifestations; peak systolic velocity (PSV) and end diastolic velocity (EDV) of vertebra artery (VA) were evaluated by colour doppler ultrasound. Results Before treatment, no significant differences on DARS and DHI scores were noted between the two groups (P>0.05); after treatment, , the DARS and DHI scores in the therapy group were 26.03±4.24 and 60.91±10.15, respectively, which were significantly decreased as compared with those in the control group (28.80±5.16 and 68.11±12.44, t=2.388 and 2.584, P=0.000); PSV and EDV of VA in the therapy group were 49.97±7.69 and 16.90±2.80, respectively, which were significantly increased as compared with those in the control group (47.31±7.47 and 15.12± 2.74, t=-2.505 and-2.631, P=0.015 and 0.011). Conclusions RLIPC could alleviate the symptoms of PCIV, which might be related to increased blood flow of VA. RLIPC is simple and safe, and can be used in the treatment of PCIV.
4.Genistein promotes the proliferation of ovarian cancer OVCAR-5 cells by upregulating Cyclin D1 and CDK4 expressions
Wen LI ; Yi LI ; Zhongwei WANG ; Hongtao REN ; Yang ZHANG ; Pengtao YANG ; Shupei PAN ; Yali WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(1):59-64
【Objective】 To explore the effect of Genistein on the proliferation and cell cycle regulation of ovarian cancer cells. 【Methods】 Ovarian cancer OVCAR-5 cells were treated with Genistein. Cell counting and MTS assays were performed to determine the alterations of cell proliferation. Real-time PCR and Western blotting were conducted to examine the expression changes of key cell cycle regulators. 【Results】 Genistein significantly promoted the proliferation and viability of OVCAR-5 cells. After Genistein treatment, cellular mRNA and protein expression levels of cell cycle activators such as PCNA, Cyclin D1 and CDK4 were increased, but those of cell cycle inhibitors such as p21 and p27 were decreased. 【Conclusion】 Genistein can upregulate the proliferation and G1-S transition of ovarian cancer OVCAR-5 cells. The discrepancy may be caused by diverged experimental conditions and/or different ER expression patterns of cell lines. The findings may provide basic information for in-depth analysis of the role(s) and mechanisms by which genistein confers its effect on ovarian cancer cells.