1.Operative management of type Ⅱ fracture of the distal clavicle
Chinese Journal of Orthopaedic Trauma 2002;0(04):-
Objective To introduce an operative method for the treatment of type Ⅱ fracture of distal clavicle. Methods The clavicle and coracoid proc ess were compressively fixed with screw in, and the coracoclavicular ligament wa s repaired in 24 cases of type Ⅱ fracture of the distal clavicle. Results The fractures healed in all the cases with good function of joint, and without screw loosening or traumatic arthritis. Conclusion The operative method is an ideal m anagement for the treatment of adult type Ⅱ fracture of distal clavicle, due t o its easy handling, reliable fixation, exact curative effects and less complica tions.
2.Relationship of k-ras mutation and colorectal cancer
Huijuan CHEN ; Hongbo LI ; Shuomin LI ; Biwen LU ; Junfeng CHENG ; Yuanli LIU
Cancer Research and Clinic 2010;22(7):461-463
Objective To explore the status of mutations of k-ras gene in colorectal cancer (CRC) patients and to make theory preparation for the k-ras mutation detection in diagnosis laboratory. Methods The Genomic DNA was extracted, mutation analysis of k-ras was detected by PCR and bi-direction sequencing in the 56 specimens. Results Rate of k-ras mutation was 46.63 % (26/56) including 76.92 % (20/26) located at codon 12, and 23.08 %(6/26) located at codon 13, and no mutation was found at both codons simultaneously. G>A transition is the most common type of k-ras mutation,GGT>GAT (G12D) is the predominant mutation at codon 12 and GGOGAC (G13D) at codonl3. Chi-square analysis revealed the k-ras mutation was significantly correlated to the gender of the patients. Conclusion The k-ras mutation is mainly located at the codon 12, G>A transition is the most type of k-ras mutation in CRC. k-ras mutation seems to correlate with the gender of CRC patients.