1.Study on intestinal absorption of multiple constituents under the drug response of Wuwei-Jiangya recipe
Huihui ZHAO ; Li YU ; Kailun ZHANG ; Changling WEI ; Yang LIU ; Baosheng ZHAO ; Shuofeng ZHANG ; Xiaoyan GAO ; Liying LIU
International Journal of Traditional Chinese Medicine 2012;34(9):804-807
ObjectiveTo research the intestinal absorption characteristics in rats of multiple constituents,when Wuwei-Jiangya recipe was used in rats and showed reducing blood pressure effects.Methods ① After extracting Wuwei-Jiangya recipe,we fed it to rats once daily,until the blood pressure reduced; ②Establish Wuwei-Jiangya recipe and intestinal absorption of multiple constituents fingerprint by using reverted gut sac method and RP-HPLC fmgerprint.ResultsAfter one week's administration,there was the hypotensive effects and multiple constituents can be absorbed by intestine.ConclusionWhen the drug works,reverted gut sac method for studying intestinal absorption constituents can be used for locking the exposure constituents.
2.Comparing studies of silybin metabolism in normal rats and liver injury model rats
Yang LIU ; Shuofeng ZHANG ; Wenning YANG ; Guoqing OUYANG ; Xiaoyan GAO ; Baosheng ZHAO ; Honghuan DONG ; Lei ZHANG ; Liying LIU
International Journal of Traditional Chinese Medicine 2012;34(8):700-702
Objective To research the metabolism and distribution ofsilybin in normal rats and liver injury model rats.Methods The normal rats group and immunity liver injury rat models were fed with the same dose ofsilybin capsule,and HPLC was used to determine the silybin concentration in biological samples in different time.Results The silybin concentration in the normal group in biological samples was higher than the model group at different time.In the normal group,the consequence of silybin concentration in each viscera distribution from top to bottom was liver>kidneys>plasma>heart,while in the model group the fact was kidneys>heart>liver>plasma.Conclusion The difference of metabolism and distribution of silybin in normal rats and liver damage model rats was obvious.
3.Development and application of bioelectric measurement system for vivo bone puncture.
Zhiyun WANG ; Qingkai DENG ; Qingshui YIN ; Jingsong GUO ; Jianbo WANG ; Shuofeng ZHAO
Chinese Journal of Medical Instrumentation 2010;34(3):164-166
Procedure of a bioelectric signal collection system for vivo critter is introduced in this paper. It is easy to measure the bioimpedance in the tip of appliance, when puncture into the tissue, especially puncture into the bone tissue. We can get a judgment on the position of appliance, thereby achieve assistance on the clinic operation.
Biopsy, Needle
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instrumentation
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Bone and Bones
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physiology
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Electrophysiological Phenomena
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Equipment Design
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Specimen Handling
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instrumentation
4.Characteristics of eye movement behavior of reading interest and comprehensibility
Jiaxin ZHAO ; Dongsen ZHANG ; Jinhui SHI ; Qiuyue SONG ; Zhaofeng HE ; Xiaomin YING ; Shuofeng HU
Military Medical Sciences 2023;47(12):919-927
Objective To identify the eye movement characteristics that can distinguish reading interest and comprehensibilityin order to provide reference for prediction of reading interest and comprehensibility based on eye movement.Methods Tobii Glasses 3 was used to collect eye tracking data from 11 participants who read 12 texts on different topics.After reading all the texts,the participants marked each sentence with theirlevels of interest and comprehensibility.Then,Python R and excel software were used to preprocess and analyze the data,and the differences in eye movementamid the four combinations of reading interest(interested or not)and comprehensibility(levels of difficulty)were studiedat overall and individual levels.Results At the overall level,there were statistically significant differences in eye movement between the easy comprehension group and the difficult comprehensiongroup where interest was concerned,but there was little difference between the interested group and the disinterested groupwhen it came to comprehensibility.There were differences in eye movement behavior between individuals,and some of the statistically significant differences in eye movement were consistent across most of the participants.In addition,some of the eye movement characteristicswere oppositeacross different individuals or could not distinguish between the interested and disinterested at the overall level,but could be distinguished within individuals.Conclusion At the overall level,it is relatively easy to determine comprehensibility through eye movement,but it is more difficult to distinguish whether the participant is interested in the sentence or not,especially when the text is easily comprehensible.At the individual level,eye movement behavior varies widely between individuals,and individuals manifest different characteristics in distinguishing reading interest and comprehensibility.
5.A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo.
Daming ZUO ; Yu CHEN ; Jian-Piao CAI ; Hao-Yang YUAN ; Jun-Qi WU ; Yue YIN ; Jing-Wen XIE ; Jing-Min LIN ; Jia LUO ; Yang FENG ; Long-Jiao GE ; Jia ZHOU ; Ronald J QUINN ; San-Jun ZHAO ; Xing TONG ; Dong-Yan JIN ; Shuofeng YUAN ; Shao-Xing DAI ; Min XU
Protein & Cell 2023;14(1):37-50
The twenty-first century has already recorded more than ten major epidemics or pandemics of viral disease, including the devastating COVID-19. Novel effective antivirals with broad-spectrum coverage are urgently needed. Herein, we reported a novel broad-spectrum antiviral compound PAC5. Oral administration of PAC5 eliminated HBV cccDNA and reduced the large antigen load in distinct mouse models of HBV infection. Strikingly, oral administration of PAC5 in a hamster model of SARS-CoV-2 omicron (BA.1) infection significantly decreases viral loads and attenuates lung inflammation. Mechanistically, PAC5 binds to a pocket near Asp49 in the RNA recognition motif of hnRNPA2B1. PAC5-bound hnRNPA2B1 is extensively activated and translocated to the cytoplasm where it initiates the TBK1-IRF3 pathway, leading to the production of type I IFNs with antiviral activity. Our results indicate that PAC5 is a novel small-molecule agonist of hnRNPA2B1, which may have a role in dealing with emerging infectious diseases now and in the future.
Animals
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Mice
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Antiviral Agents/pharmacology*
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COVID-19
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Hepatitis B virus
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Interferon Type I/metabolism*
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SARS-CoV-2/drug effects*
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B/antagonists & inhibitors*