Objective To research the effect of Dingxin capsule on the level of TXB2 and MMP-9 in rats, and explore the mechanism of treating and preventing atrial fibrillation (AF) by TCM. Method AF animal model was established by injecting the calcium chloride-acetylcholine mixed liquid in coccygeal vein of rats. The rats with typical ECG were divided randomly into 4 groups:AF model group (M-group), Dingxin capsule group (D-group), verapamil group (V-group), normal group (N-group). TXB2 and MMP-9 were measured 4 weeks later. Result Comparing with M-group, the level of TXB2 and MMP-9 of D-group decreased obviously (P

Objective The relationship between XbaI polymorphism of ApoB gene and cerebral infarction(CI)among Chinese population was assessed by Meta-analysis.Methods All related case-control studies were collected from all publications,the relevant studies were identified after eliminating those poor-qualified studies.Meta-analysis was conducted for investigating heterogeneity among individual studies,and summarizing the effects across studies.Results The combined data statistics revealed the frequencies of the X-X+/X+X+ genotypes showed no statistically difference(Z=1.72,P=0.08).Through the subgroup analysis,it was obviously increase the risk of CI in Chinese northern population(OR=2.66,95%CI:1.67~4.24),but no statistically difference in Chinese southern population(OR=1.26,95%CI:0.90~1.77).Conclusions ApoB gene XbaI polymorphism may be significantly associated with susceptibility of CI in Chinese northern population,but not a definite risk for Chinese southern population.

Objective To observe the effects of recombinant staphylokinase (r-SAK) on platelet activation parameters in patients with acute myocardial infarction(AMI) by intravenous thrombolysis in order to investigate the clinical thrombolytic efficacy of r-SAK therapy in AMI comparing with recombinant tissue-type plasminogen activator(rt-PA) therapy.Methods Thirty-three patients with AMI within 12 h after the onset were selected and divided randomly into the r-SAK therapy group(n=17) and rt-PA therapy group(n=16).Coronary artery angiography(CAG) was performed 90 min after thrombolytic therapy in patients.Thrombin-antithrombin complex(TAT) and alpha granule membrane protein(GMP-140) were measured by similar commercial enzyme-linked immunosorbent assay(ELISA).Results In r-SAK group and rt-PA group,the plasma contents of GMP-140 2 h after thrombolytic therapy were significantly higher than before therapy(P0.05).In rt-PA group,the plasma content of TAT 2 h after thrombolytic therapy increased significantly(P0.05).) Conclusion r-SAK has similar effect with rt-PA and it will become available for highly fibrin-selective thrombolytic therapy of AMI.Thrombolytic treatment with r-SAK can improve the injury of myocardial microperfusion.

As a rising automatic identification technology, radio frequency identification (RFID) is receiving considerable attention and eliciting widespread interest in medical institutions in China. Some medical institutions have begun exploring and developing RFID systems for kinds of applications. A majority of medical institutions pay insufficient attention to the issue of RFID interference with medical devices. Specialized standards for the application of RFID in healthcare environments are desired. And there are misalignments between existing technology standards. Further researches should be developed to study the influence on safety and efficiency of medical devices induced by RFID system.

Objective To investigate the clinical features and treatment of mycoplasma pneumoniae infection complicated with allergic purpura.Methods 40 children with mycoplasma pneumoniae infection complicated with allergic purpura were selected.Clinical symptoms,disease treatment and outcome were analyzed retrospectively.Results 25 cases were abdominal type allergic purpura,9 cases were articulated joints allergic purpura,6 cases were kidney patients with allergic purpura.In addition,some patients showed upper or lower respiratory tract infections.All the patients received blood circulation improvement,anti-allergy and other conventional treatment,and on the basis of this,azithromycin,low-dose dexamethasone or gamma globulin was given.All 40 patients were cured,the average length of hospital stay was (18.6 ± 3.1) d.Conclusion Mycoplasma pneumoniae infection may trigger allergic purpura purpura-like rash,the clinical symptoms,some accompanied by lower respiratory infections,should give the conventional anti-mycoplasma pneumoniae infection treatment,and the treatment time should be sufficient to cure allergic purpura,shortening hospitalization time,has an important role in the prevention of recurrent disease.

Objective To investigate ASPS induced G_2/M arrest in lung cancer cell line H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis was used to inspect the proteins of ERK, p-ERK. Results Compared with control group, G_2/M phase cells increased with concentration significantly, G_0/G_1 phase cells were not different, G_2/M phase cells and G_0/G_1 phase cells were not different when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, protein of p-ERK was significantly increased, expression of ERK was no different. Conclusion ASPS may induce G_2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.

Objective To analyze the clinical and pathological features of collecting duct carcinoma (CDC) of the kidney. Methods 11 patients with CDC were analyzed,among which 6 were males and 5 were females.Their age ranged from 22 to 67 years old with a mean age of 55.4 cases were found by routine health examination,4 cases were presented with gross hematuria and 3 cases had flank or abdomen pain.The CT scan showed an unclear boundary mass in kidney,with tumor sizes from 2.1 to 8.5 cm ( mean 5.6 cm).Only medullary involvement was present on CT in 3 cases,Medullary and cortical involvement in 5 cases,Medullary and pelvic involvement in 2 cases,and all involvement in 1 case.Infiltrative lesion has expanded kidney but maintains reniform contour.Contrast-enhanced CT scan showed lesion mild to moderate enhancement compared with surrounding parenchyma. Results Radical nephrectomy was performed in 8 cases and palliative nephrectomy in 3 cases.All patients were finally diagnosed by pathology.Grossly,the tumor is often appears gray or white.In HE staining,tumor showed prominent tubular or tubulaopapillary structures with desmoplasia and inflammatory reaction.Occasionally,some tumor cells take on a hobnail appearance.Immunohistochemical examination showed UEA-1 positive in all cases,EMA positive in 9 cases,PNA positive in 8 cases,and HMW-CK positive in 7 cases.Only 2 patients showed positive CD10.7 patients died within 6 to 47 months (mean 12.5 months),2 survived with tumor free for 9 months and 8 years respectively,one lost of follow-up,and one patient with distant metastasis is receiving postoperative chemotherapy. Conclusions Collecting duct carcinoma of the kidney is a rare histological type of renal cell carcinoma,whose final diagnosis depends on histopathological examination.Rapid progression,highly malignant,poor prognosis are the characteristics of this disease.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; Phytotherapy

Objective To evaluate the quality of Hedysari Radix from different producing areas in Gansu Province by setting Hedysari Radix from different producing areas in Gansu Province as study subjects and trace elements in Hedysari Radix as evaluation indexes; To provide the references for establishment of the quality standards of Hedysari Radix.Methods The content of Fe, Cu, Ca, Mn, Zn, Mg, Cr, Co, Na, Li, Ni and K in Hedysari Radix were detected by using flame atomic absorbption method. Data were analyzed by the factor analysis conducted by SPSS21.0 software. The common factors were extracted by principal component and analytic function for quality comprehensive ranking was established.ResultsThree common factors were extracted, namely F1, F2, and F3. The quality function was Y=0.657F1+0.119F2+0.089F3. Cr, Li, Co, Cu, Zn, Fe and Mg were the characteristic trace elements of Hedysari Radix that could affect the quality of Hedysari Radix. When the quality of Hedysari Radix was evaluated by the contents of trace elements, the quality of wild Hedysari Radix were better than cultivated Hedysari Radix and the quality rank was consistent with the idea that Longnan is the main producing area of Hedysari Radix. The quality of samples from Dingxi area was better.Conclusion The quality evaluation of Hedysari Radix based on trace elements shows the differences in Hedysari Radix from different producing areas in Gansu Province and the results are relatively reasonable.

ABSTRCT OBJECTIVE:To analyze the correlation between contents of active ingredients in Hedysarum polybotrys and ecologi-cal factors. METHODS:HPLC was used for the simultaneous determination of calycosin and formononetin in H. polybotrys;UV spectrophotometry was used to determine the content of hedysari polysaccharide;and hot-dip method was used to determine the con-tent of extracts in H. polybotrys. Factor analysis method was used to analyze the correlation between contents of active ingredients and ecological factors. RESULTS:4 common factors,which were heat,geography,illumination and precipitation,were extracted. The heat was an ecological dominant factor influencing the quality of H. polybotrys. And ≥10 ℃ accurnulated temperature was the most significant variate influencing hedysari polysaccharide,latitude was the most significant variate influencing the contents of ca-lycosin and formononetin H. polybotrys;and annual averge amount of radiation was the most significant variate influencing content of extracts in H. polybotrys. CONCLUSIONS:Different ecological factors have different effects on the contents of active ingredi-ents in H. polybotrys,and it is the result of the combined effect of various of ecological factors.