Objective To explore the influence of carbamazepine on the plasma homocysteine (Hcy),folic acid and vitamin B12 levels in the treatment of epilepsy.Methods The clinical medical history and medication com-pliance of 80 epilepsy patients were retrospectively analyzed.40 cases treated with carbamazepine were set as group A,another 40 cases without taking antiepileptic drug therapy were set as group B.And 40 cases in the same period in hospital physical examination were selected as control group.The levels of blood Hcy,folate and vitamin B12 were detected by using the fluorescent quantitative method,the electrochemical luminescence immunoassay,and the differ-ences of three groups of the levels were compared.Results The Hcy level of group A was significantly higher than the control group and group B(F =7.16,P =0.00),and the folic acid level of group A was significantly lower than the control group and group B(F =9.67,P =0.00).The Vit B12 concentration of group A was lower than the control group and group B,but among the three groups,the difference was not statistically significant(F =1.05,P =0.15). Loss of appetite and body weight decreased in 4 cases,2 cases of dizziness,fatigue,but symptoms were mild,did not affect the findings.Control in 13 cases,12 cases were markedly effective,effective in 8 cases,ineffective in 7 cases,the total effective rate was 82.5%.Conclusion Carbamazepine treatment can cause epilepsy patients blood Hcy level rise,and folic acid level drop,has no significant effects on the Vit B12 concentration,so in patients with epilepsy should pay attention to monitor blood Hcy and folic acid level during the treatment,in order to avoid adverse events such as thrombosis and influence the prognosis.