OBJECTIVETo observe therapeutic effect of acupuncture at Zhaohai (KI 6) and Shenmai (BL 62) on insomnia.

METHODSSeventy-eight cases of insomnia were randomly divided into a treatment group of 40 cases and a control group of 38 cases. The treatment group were treated with acupuncture at Zhaohai (KI 6) using reinforcing method and at Shenmai (BL 62) using reducing method, combined with acupuncture at acupoints selected according to syndrome differentiation. The control group were treated with acupuncture at acupoints selected according to syndrome differentiation. Their therapeutic effects were compared.

RESULTSThe cured rate and the total effective rate were 62.5% and 97.5% in the treatment group, and 31.6% and 68.4% in the control group, respectively, with significant difference between the two groups (P < 0.01).

CONCLUSIONAcupuncture at Zhaohai (KI 6) and Shenmai (BL 62) has a better therapeutic effect on insomnia.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Sleep Initiation and Maintenance Disorders ; Syndrome

Objective To explore the risk factors of children with cerebral palsy during pregnancy and neonatal period in Xinxiang area.Methods A retrospective analysis of the relevant research data of cerebral palsy children in Xinxiang area was performed.The research objects were children with cerebral palsy born from May 1,2005 to April 30,2010.At the same time,3 healthy children were selected as the control group to analyze the related risk factors causing cerebral palsy in children.Results The risk factors of children with cerebral palsy in Xinxiang city were as follows : maternal nutritional status, vaginal bleeding during pregnancy, pregnancy-induced hypertension syndrome, and abnormal production history were associated with cerebral palsy (x2 =2.313,13.296,5.034,7.434, all P ＜ 0.05)during the perinatal period;related factors during neonatal period were premature birth,severe asphyxia, severe jaundice, and intracranial infection(x2 =4.637,29.50,4.633,5.107, all P ＜ 0.05).Multivariate Logistic regression analysis showed the history of severe asphyxia (OR =2.340,95 ％ CI: 1.250-4.440), severe jaundice (OR =4.110, 95％ CI:2.430-6.740) ,premature birth(OR =2.570,95％ CI: 1.410-4.770) ,pregnancy-induced hypertension syndrome (OR =2.350,95 ％ CI:I.020-5.440), vaginal bleeding during pregnancy (OR =73.600,95 ％ C1:3.060-17.750) and abnormal production history(OR =5.710,95％ CI: 1.250-26.310) were independent risk factors causing children with cerebral palsy.Conclusions The history of severe asphyxia, severe jaundice, premature birth, pregnancy-induced hypertension syndrome, vaginal bleeding during pregnancy and congenital dysplasia are independent risk factors of children with cerebral palsy in Xinxiang area.It should be strengthened to screen and standardize the management of high-risk newborn infants with cerebral palsy, and do well management for perinatal high-risk pregnant women management.Early prevention can help to reduce the incidences of cerebral palsy in local area.

Objective To explore the pathogenesis of juvenile idiopathic arthritis (JIA) and its potential molecular targets.Methods Gene microarray data about JIA was downloaded from Gene Expression Omnibus (GEO) in NCBI.Different expressed genes were identified through bioconductor packages in R programing.The causal genes from the different expressed genes were optimized and replenished by GLAD4U and ToppGene.Functional annotation information of causal genes was carried out by DAVID.Results List of causal genes was obtained as well as their enriched function.Some potential pathways such as Cytokine-cytokine receptor interaction,Jak-STAT signaling pathway,which may be important in the progression of JIA were screened.Conclusions The pathogenesis of JIA,as well as its potential causal genes,can be captured through bioinformatics methods,so it may be used as time and cost saving method in experimental study and clinical application.

AIM: To observe the effect of Dioscin treatment on NF-κB signaling pathway and cellular inflammatory injury and explore its potential mechanism in uric acid-induced mouse tubular epithelial cells (mTECs). METHODS: After 1.2 mol/L uric acid induced mTECs, Dioscin and NF-κB P65 inhibitor BAY11-7082 were given to intervene respectively. IκB-α, NF-κB P65, PP65, NLRP3, IL-1β and β-actin were detected by Western Blot, immunofluorescence staining and real-time PCR. RESULTS: Western Blot, immunofluorescence staining and real-time PCR analysis showed that expression levels of PP65, NLRP3 and IL-1β were significantly downregulated in the uric acid-induced mTECs with Dioscin and BAY11-7082 treatment. CONCLUSION: Dioscin attenuates uric acid-induced cellular inflammatory damage by suppression NF-κB signaling pathway.

OBJECTIVE: To observe the effect of conventional acupuncture combined with row-like puncture at sternocleidomastoid on peripheral facial palsy at recovery stage. METHODS: A total of 60 patients with peripheral facial palsy at recovery stage were randomized into an observation group and a control group, 30 cases in each one. Acupuncture was applied at affected Cuanzhu (BL 2), Yangbai (GB 14), Sibai (ST 2), Quanliao (SI 18), Jiache (ST 6), Dicang (ST 4), Hegu (LI 4), Taichong (LR 3) and Zusanli (ST 36) in the control group. On the basis of the treatment in the control group, row-like puncture was applied at sternocleidomastoid (1 needle was punctured at muscle origin and insertion respectively, 3 to 4 needles were row-like punctured at the connection line of muscle origin and insertion). The treatment was given once a day, 5 times were as one course, with 2-day interval, totally 4 courses were required in the both groups. The house-brackmann (H-B) facial nerve function grade, facial nerve function rating system-dynamic view rating scale score and facial disability index (FDI) scale score [including scores of FDI physical function (FDIp) and FDI social life function (FDIs)] before and after treatment were observed, and the clinical efficacy was evaluated in the two groups. RESULTS: After treatment, the H-B facial nerve function grades were improved compared before treatment in the both groups ( CONCLUSION Compared with conventional acupuncture, combination therapy with row-like puncture at sternocleidomastoid can improve the therapeutic effect of peripheral facial palsy at recovery stage.
Acupuncture Points ; Acupuncture Therapy ; Facial Paralysis/therapy* ; Humans ; Needles ; Punctures ; Treatment Outcome

Objective To conduct the epidemiological investigation and analysis of cerebral palsy in Xinxiang of Henan Province and to investigate its risk factors in order to provid a basis for further study of etiology and prevention of cerebral palsy information.Methods Cluster sampling survey was carried out among children aged 1-6 years in XinXiang,Henan Province,and the data were analyzed by using SPSS 13.0 statistical analysis software.Results The morbidity of infantile cerebral palsy in Xinxiang of Henan Province was 2.82‰.The prevalence distribution in all age groups was 2.46 ‰-3.11‰(x2 =0.374,P =0.996),and the prevalence rate in male and female was significantly different(x2 =0.139,P =0.709) ; the sex ratio was 1.09 ∶ 1.00.Prevalence rate was slightly lower in urban areas than in rural areas (x2 =0.526,P =0.769).But no significant differences were observed in all of the data above.The incidence of cerebral palsy of children whose mothers did not established perinatal care manual and guidance during pregnancy was 5.86 times of the children whose mothers established perinatal care manual and guidance (x2 =116.806,P =0.000) ;the incidence of cerebral palsy in children whose mothers did not receive regular prenatal care during pregnancy was 5.37 times of the children whose mothers receive regular prenatal care during pregnancy (x2 =43.904,P =0.000);the incidence of cerebral palsy in children who had no neonatal follow-up after birth was 8.55times of the children with neonatal follow-up after birth (x2 =68.987,P =0.000).The incidence of cerebral palsy in children whose developmental disorders were not timely diagnosed and treated medically was 5.39 times the children whose developmental disorders were timely diagnosed and treated (x2 =56.003,P =0.000).The significant differences were observed in all of the data above.In the classification of cerebral palsy,the spastic type was the most (42.1％) ;followed by the dyskinetic (24.6％) ; the mixed (18.8％) ; and the ataxia(14.5％).Conclusions The survey results can reflect current prevalence of infantile cerebral palsy in children aged 1-6 years in XinXiang,and can be served as a basis for further prevention and treatment of cerebral palsy information.

Objective An experiment study on the femoral head plastic operation to prevent and treat aseptic necrosis of the femoral head. Methods 60 Chinese white rabbits were equally random divided into 4 groups. The four groups except A group were made a globose defect and filled with 95% alcohol tampon for 30 minutes. The B group was natural repair group. The C group was filled with bone cement after being made defect. The D group was filled with bone cement added barium sulfate agent after being made defect. After 12 weeks, all rabbits in four groups were killed. The specimens were random divided into two parts. The articular cartilage was observed and measured immediately. The other specimen was determined with histological examination and extreme anti-pressure rigidity test . Result The femoral heads of bone cement group and the femoral heads of contrast agent bone cement group kept their outline all the time through the articular cartilage observation. After 12 weeks the cartilage thickness of natural repair group (511.74 ± 69.00) was thinner than the other three groups [ control group ( A group ) (511.74 ± 69 00 );Bone cement group ( C group) (468. 36 ± 82. 99 ); Bone cement group contrast agent ( D group ) ( 515.61 ±64. 65 ) ], and it had significant difference ( P < 0. 05, P < 0. 01 ). The rigidity of natural repair group [ (676. 57 ±50. 92) N/mm] had significant difference with that of the other three groups[ A group( 884. 66±52. 29)N/mm;C group(1178.40 ± 170.45) N/mm;D group(928.60 ± 104.42) N/mm] ( P <0.05, P<0. 01 ). Conclusions Femoral head plastic operation was simple and it could release pain. And femoral head outline could be kept integrity. It would be a new operation which was used to treat young patients in Ficat Ⅱ～Ⅲ period to postpone or substitute total hip replacement.

AIM: To study the effect of acupuncture treatment on intraocular pressure(IOP)control, corrected visual acuity, light sensitivity and visual fields mean defect in patients with glaucoma beside the original treatment.

METHODS:Totally 48 cases(62 eyes)with glaucoma whose IOP were under control were randomly divided into two groups, the observation group and control group, from January to October 2016. Patients in control group were treated with the original therapy for 3 courses, while observation group were treated with acupuncture therapy for 3 courses, 12d as one course, beside original therapy. Corrected visual acuity, light sensitivity and visual fields mean defect of the two groups before and after treatment were analyzed.

RESULTS: Corrected visual acuity, light sensitivity and visual fields mean defect of control group had no differences between before and after treatment by paired t test(P>0.05), those of observation group were significantly different(P<0.05). The three examinations were significantly better of observation group than those of control group after treatment by independent t test(P<0.05). The total effective rate of observation group was 74%, which was significantly better than that of control group(32%)(χ² = 9.329, P < 0.05).

CONCLUSION: For glaucoma patients, whose IOP were under control, acupuncture has some improvement on corrected visual acuity, light sensitivity and visual fields mean defect, and is better than single IOP-control therapy in short term effects, but a long-term effect is needed to be studied.

Objective To explore the effect of age on the fracture healing through bioinformatical analysis of gene ex?pression data in GEO, and to screen critical molecular targets and pathways involved in this process. Methods Through R programming language, we identified different expressed genes between 26/52 week old rats and 6 week old rats in every time points of the experiment (No fracture;3 days, 1 week, 2 weeks, 4 weeks and 6 weeks after fracture). By comparison of these different expressed genes, those genes that may contribute to fracture healing were identified. Function annotation was conducted based on DAVID database and PPI network that was constructed via STRING database. Results Compared with 6 week old rat, 52 week old rat show more different genes at 2, 4 and 6 weeks after fracture as well as more than intact rats. At the time point of 6 weeks after fracture, 26 week old rat present 4 different genes while 52 week old rat present 99 differ?ent genes compared with 6 week old rat. We totally found 99 genes that might play important roles in the process of fracture healing. These genes involved in biological process related to bone healing, immune, inflammatory and etc. Also, two screened gene enriched KEGG pathways were identified: ECM-receptor interaction and Arachidonic acid metabolism. Through the analysis of PPI network, Pcna, Fn1, Casp3 and etc, who present high density connectivity in PPI network, were screened out. Conclusion Pcna, Casp3 and Fn1 and etc might play important roles in fracture healing through affecting ECM-receptor interaction and Arachidonic acid metabolism.

AIMTo study the effects of ginkgolide B on lipopolysaccharide (LPS)--induced TNFalpha production in mouse peritoneal macrophages and NF-kappaB activation in rat pleural polymorphonuclear leukocytes.

METHODSL929 crystal violet staining assay was used to show the level of TNFalpha released from mouse peritoneal macrophages induced by LPS. Electrophoretic mobility shift assay (EMSA) was used to determine NF-kappaB binding activities.

RESULTSGinkgolide B (1, 10 micromol x L(-1)) was shown to significantly inhibit LPS (10 mg x L(-1))-induced TNFalpha production in mouse peritoneal macrophages, the IC50 was 0.26 micromol x L(-1); LPS (1 mg x L(-1)) and PAF (1 nmol , L(-1)) were shown to increase the NF-kappaB binding activities in rat pleural polymorphonuclear leukocytes; ginkgolide B (10 micromol x L(-1)) was found to inhibit LPS (1 mg x L(-1))-induced NF-kappaB activation in rat pleural polymorphonuclear leukocytes; ginkgolide B (1, 10 micromol x L(-1)) was shown to inhibit PAF (1 nmol x L(-1))-induced NF-kappaB activation in rat pleural polymorphonuclear leukocytes.

CONCLUSIONThe inhibition of NF-kappaB activation and TNFalpha production might be considered to be part of the mechanisms underlying the antiinflammatory action of ginkgolide B; PAF is involved in activation of the NF-kappaB pathway stimulated with LPS.

Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Diterpenes ; isolation & purification ; pharmacology ; Female ; Ginkgo biloba ; chemistry ; Ginkgolides ; Lactones ; isolation & purification ; pharmacology ; Lipopolysaccharides ; antagonists & inhibitors ; Macrophages, Peritoneal ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; NF-kappa B ; metabolism ; Neutrophils ; enzymology ; Plants, Medicinal ; chemistry ; Platelet Activating Factor ; antagonists & inhibitors ; Rats ; Rats, Wistar ; Tumor Necrosis Factor-alpha ; biosynthesis