1.Carvedilol attenuates CPB-induced apoptosis in dog heart: regulationof Fas/FasL and caspase-3 pathway.
Shunye ZHANG ; Zongquan SUN ; Lixin LIU ; Hasichaonu
Chinese Medical Journal 2003;116(5):761-766
OBJECTIVETo evaluate the effects of Carvedilol on cardiopulmonary bypass (CPB)-induced myocardiocyte apoptosis and its effects on regulation of Fas, FasL expression, caspase-3 activity and oxidative stress in the left ventricle (LV) in this setting.
METHODSTen adult dogs undergoing conventional hypothermic CPB were randomly divided into control and Carvedilol treated groups (n = 5, respectively). Dogs in Carvedilol treated group received a bolus of Carvedilol (1 mg/kg) intravenously and a maintenance dosage of Carvedilol (3 micro g.min(-1).kg(-1)) for 3 hours after the reperfusion of the heart. Dogs in control group received no Carvediolol. LV samples were obtained before, during and 3 hours after CPB. In situ nick end-labeling (TUNEL) technique was used to detect the apoptotic cells. The expressions of Fas and FasL were detected immunohistochemically and quantified by fluorescence activated cell sorting (FACS). The activity of caspase-3 enzyme and malondialdehyde (MDA) level were measured by cleavage of Z-DEVD-AMC substrate and thiobarbituric acid reactive substance (TBARS) method, respectively.
RESULTSBefore and during CPB, all the parameters were not significantly different intra- or between groups (P > 0.05). After CPB, these parameters in both groups were significantly elevated compared with those of before and during CPB (P < 0.028, respectively). However, the number of apoptotic cells in Carvedilol treated group was significantly decreased compared with that of the control group (P < 0.021). The expressions of Fas and FasL were significantly downregulated by Carvedilol (P < 0.001 and 0.003, respectively). The caspase-3 activity and the content of MDA in the Carvedilol treated group was also significantly reduced (P < 0.026 and 0.005, respectively).
CONCLUSIONSCarvedilol significantly reduces CPB-induced cardiomyocyte apoptosis in dog hearts and the reduction of cardiomyocyte apoptosis is associated with downregulation of Fas and FasL expression, inhibition of caspase-3 activity and oxidative stress in LV.
Adrenergic beta-Antagonists ; pharmacology ; Animals ; Apoptosis ; Carbazoles ; pharmacology ; Cardiopulmonary Bypass ; Caspase 3 ; Caspases ; metabolism ; Dogs ; Down-Regulation ; Fas Ligand Protein ; Female ; In Situ Nick-End Labeling ; Male ; Membrane Glycoproteins ; metabolism ; Myocytes, Cardiac ; cytology ; metabolism ; Propanolamines ; pharmacology ; Signal Transduction ; fas Receptor ; metabolism
2.Prognostic analysis on unprotected left main coronary artery disease and/or three-vessel coronary disease after different revascularization methods
Pengjie YANG ; Xuebin HAN ; Lijun ZHU ; Yongzhi DENG ; Shunye ZHANG ; Jicheng XI ; Xiaolong ZHANG ; Jingping WANG ; Jin DONG ; Jian AN
Chinese Journal of Thoracic and Cardiovascular Surgery 2017;33(5):306-308
3.Assessment of different orchiectomy for patients with advanced prostate cancer
Yuan GAO ; Shunye SU ; Ludong LIU ; Yunjiang ZANG ; Shenyang WANG ; Mingrong ZHANG ; Quan ZHOU ; Shaowen ZENG ; Liang QIAO
Journal of Endocrine Surgery 2011;05(5):340-342
Objective To compare epididymis-sparing orchiectomy (group A) with traditional orchiectomy (group B ) in patients with advanced prostate cancer,and to evaluate which procedure is better.Methods A total of 60 cases of advanced prostate cancer patients were enrolled,with 30 cases in group A and 30 cases in group B.They were given oral anti-androgen from 1 day after castration.Serum level of testosterone and prostatic specific antigen (PSA) was detected before castration,and 1 week,1,3,6,9 and 12 months after castration.Patient satisfaction was also evaluated.Results On time point of 12 months after castration,the average level of serum testosterone was 0.2 nmol/L (95 % confidence interval,0.1 ~ 0.9 nmol/L) in group A and 0.3 nmol/L (95% confidence interval,0.2 ~ 0.9 nmol/L) in group B (P >0.05 ) ; the average value of PSA was 0.22 ng/ml in group A and 0.27 ng/ml in group B (P >0.05 ) ; patient satisfaction rate was 96.7% (29/30) in group A and 53.3% (16/30) in group B.Conclusions No significant difference of testosterone level and PSA is found between the 2 groups.However,epididymis-sparing orchiectomy meets the psychological needs better because it helps to maintain the appearance of the scrotum through epididymis preservation and epididymoplasty.
4.Single ventricle separation in 1 case
Zhibiao ZHANG ; Jicheng XI ; Chongjun WANG ; Bin YAO ; Shunye ZHANG ; Yongzhi DENG ; Weiwei CHENG ; Yong DOU
Chinese Journal of Thoracic and Cardiovascular Surgery 2017;33(11):691-692
5.Regulatory effect of bcl-2 family proteins in CPB-induced cardiomyocyte apoptosis in dog hearts.
Zongquan SUN ; Shunye ZHANG ; Lixin LIU ; Hasichaolu
Journal of Huazhong University of Science and Technology (Medical Sciences) 2002;22(2):103-106
Whether conventional hypothermic CPB induces myocyte apoptosis in dog hearts and modulation of bcl-2, bcl-xl, bax, bad, and caspase-3 pathways in this setting was investigated. Ten healthy adult dogs were randomized into sham-operated and CPB groups. Samples of left ventricle were obtained before, during and 3 h after CPB. In situ TUNEL was used to detect apoptotic myocytes. Immunohistochemistry and flow cytometry were employed for detection of expressions of bcl-2, bcl-xl, bax and bad proteins. Z-DEVD-AMC substrate cleavage and TBARS methods were used to measure the activity of caspase-3 and the content of lipid peroxide in LV myocardium, respectively. After CPB, the number of apoptotic myocytes in CPB group was significantly increased. The results of immunohistochemistry demonstrated that bcl-2, bcl-xl, bax and bad proteins were constitutionally present on the sarcolemma of the LV myocytes. FACS results showed that, after CPB, expressions of bax and bad in CPB group were significantly upregulated, while the expressions of bcl-2 and bcl-xl were not significantly changed in both groups. The activity of caspase-3 and the content of lipid peroxide in LV myocardium in CPB group were also significantly increased after CPB. The present study shows that there exists myocardiocyte apoptosis in dog hearts undergoing conventional hypothermic CPB and the myocyte apoptosis is initiated by ischemia and performed during reperfusion. Moreover, the CPB-induced myocyte apoptosis was associated with upregulation of expressions of bax and bad proteins, activation of caspase-3 and increase of oxidative stress.
Animals
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Apoptosis
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Cardiopulmonary Bypass
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Caspases
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metabolism
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Dogs
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Female
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Hypothermia, Induced
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Lipid Peroxidation
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Male
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Myocytes, Cardiac
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metabolism
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pathology
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Proto-Oncogene Proteins
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genetics
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metabolism
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Proto-Oncogene Proteins c-bcl-2
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genetics
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metabolism
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Random Allocation
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bcl-2-Associated X Protein
7.Global risk classification in patients undergoing percutaneous or surgical complex coronary artery disease revascularization
Pengjie YANG ; Bao LI ; Yijun XU ; Zhiqiang CHEN ; Peng LIU ; Jingping WANG ; Jian AN ; Jin DONG ; Shunye ZHANG ; Yongzhi JICHENG ; Deng XI
Chinese Journal of Thoracic and Cardiovascular Surgery 2017;33(9):564-566
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