OBJECTIVETo analyze isofraxidin and its metabolites in rat plasma, bile, urine and feces after the oral administration of A. senticosus extracts.

METHODAfter the oral administration of 325 mg x kg(-1) of A. senticosus extracts, rat blood samples were collected at 1, 1.5, 2, 4, 6 h and bile samples were collected during 0-12 h. The above samples were prepared by SPE. Their urine and feces samples were collected during 0-12 h and 12-24 h. These samples were analyzed by UPLC-Q-TOF-MS and processed using Metabolynx XS.

RESULTM0 was detected in rat plasma, bile, urine and feces, isofraxidin glucuronide (M1) was detected in rat plasma and bile, which was first reported as the metabolite of isofraxidin.

CONCLUSIONIsofraxidin can be metabolized in the form of glucuronidation in vivo and evacuated in the form of isofraxidin.

Animals ; Chromatography, High Pressure Liquid ; Coumarins ; blood ; metabolism ; pharmacokinetics ; Drugs, Chinese Herbal ; metabolism ; pharmacokinetics ; Eleutherococcus ; chemistry ; Male ; Mass Spectrometry ; Rats ; Rats, Wistar ; Statistics as Topic