OBJECTIVETo investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.

METHODThe content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.

RESULTActive ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).

CONCLUSIONActive ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.

Antioxidants ; pharmacology ; Cell Culture Techniques ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; Flavanones ; analysis ; genetics ; radiation effects ; Flavonoids ; analysis ; genetics ; pharmacology ; radiation effects ; Free Radical Scavengers ; pharmacology ; Light ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods ; Plant Extracts ; chemistry ; Rehmannia ; chemistry ; Scutellaria baicalensis ; chemistry ; growth & development ; Suspensions ; chemistry

Objective:To understand the genetic variations of the coding gene of the main neutralizing antigen VP7 of G9 group A rotavirus in Xiamen area and the difference between VP7 gene in some domestic and broad areas. Methods:Four types G9 group A rotavirus strains were collected from feces of children with diarrhea in Xiamen Children′s Hospital. The time of collection was October 5, 2017, November 12, 2017, December 7, 2017 and January 15, 2018, respectively. The full-length sequence of type G9 group A rotavirus VP7 gene was obtained by reverse transcription polymerase chain reaction, and the homology, phylogenetic and amino acid sequence alignment were analyzed by using DNA Star, MEGA and other biological software. Results:Phylogenetic tree analysis showed that the local strain of Amoy CHINA/2018 in Xiamen area and the human/SC7/CHN/2013/G9 local strain in Chengdu City had the most closely relationship in the evolutionary tree clusters, and the homology was far from the Hu/JS2016 local strain in Jiangsu Province. The amino acid sequence analysis showed that compared with the reference strain WI61, the Xiamen local strains had variations in the amino acid primary structure, including D100N and Y144H, which were representative mutation sites in the Amoy CHINA/2018 phylogenetic tree cluster.Conclusion:The full-length genome sequence of the type G9 group A rotavirus VP7 shows that the strain is mutated in China.

Objective In response to the new landscape of the DIP payment reform,this study uses a public tertiary comprehensive hospital in Jingzhou as a case study.By focusing on disease analysis,it explores refined operational management strategies to promote the hospital's transformation,upgrade,and operational efficiency.Methods Starting from July 2022,a problem-oriented approach was employed to analyze the characteristics of disease structure using methods such as the Boston Ma-trix,Pareto Analysis,and the Onion Method.A multidisciplinary operational management model was adopted to provide targeted supervision to departments for improving diagnosis and treatment processes,optimizing disease types and cost structures,and im-plementing refined management.Results The sample departments showed continuous growth in medical service capabilities,with the number of disease types increasing by 5.76％year-on-year and the number of discharges rising by 20.9％.The CMI val-ue for DIP-related drug consumption increased by 0.22.The economic structure improved significantly,with average drug costs per inpatient decreasing by 14.19％and average material costs dropping by 30.62％.Medical insurance settlements saw substan-tial improvement,with losses reduced by over 80％.Operational efficiency was notably enhanced,with the average length of stay decreasing by 1.0 days year-on-year.Patient experience improved,with satisfaction rates exceeding 97％.Conclusion The re-fined operational management model based on disease analysis under the DIP framework can facilitate efficient hospital operations,promote rational use of medical insurance funds,and effectively reduce patient burdens,leading to a win-win situation for all stakeholders.