Twenty-eight adult male rats were used for this study. The experimentar were injected intramuscular with 25 mg/d cortisone acetate and were killed in various intervals separately. The neurohypophysis were fixed in mixed solution of 4% paraformaldehyde and 2% glutaraldehyde in buffered cacodylate. Frozens ections were incubated in the medium of sodium glycero-phosphate and lead nitrate, postfixed in caulfield solution, dehydrated, embedded, ultrathin sectioned, stained With uranium acetate without lead citrate, and observed under EM, In the neurohypophysis of control animals, the active areas of acid phosphatase were few and were limited in lysosome and Golgi apparatus of pituicytes and endothelia of capillaries. After injection of cortisone, the lysosome of pituicytes were increased and the activity of acid phosphatase were strengthened. Besides pituicytes and endothelia cells, there were so many positive thin granules in the processes of dark pituicytes, some areas of positive reaction of AcP appeared in neurosecretion fibers. After increasing the drug dosage, positive reaction of AcP areas showed a tendency of expansion. In this study, we discussed the effect of cortisone on the AcP of neurohypophysis and the functional significance of pituicytes in neurosecretion procedure.

Objective To investigate gender differences in mortality after percutaneous coronary intervention(PCI) in contemporary "real world" practice and determine whether female gender is an independent risk factor for PCI mortality.Methods A total of 2 493 patients including 1 860 men(male group) and 633 women(female group) who underwent PCI during July 1st,2004 to September 30st,2005 in Beijing Anzhen Hospital were retrospectively investigated and followed up.The risk factors before PCI and the mortality rates post PCI were compared.Results Women undergoing PCI were older and had more comorbidities than men.However,men were more likely to have depressed ejection fraction,prior myocardial infarction,and prior coronary revascularization.Women demonstrated a ten-fold higher in-hospital mortality rates compared with men(1.1% vs 0.1%,P

Purpose To investigate the clinicopathological features and prognosis in patients with colorectal cribriform comedo-type ad-enocarcinoma. Methods Compared with 278 cases of colorectal common-type adenocarcinoma, 24 cases of colorectal cribriform come-do-type adenocarcinoma were analyzed for clinical stage, lymphovascular invasion, lymph node metastasis and genetic characteristics. Results The percentage of clinical Ⅲ and Ⅳ (87. 5%) and lymph node metastasis rate (87. 5%) in patients with colorectal cribri-form comedo-type adenocarcinoma was obviously higher than that in colorectal common-type adenocarcinoma (42. 4%,42. 4%) (P<0. 05). The percentage of lymphovascular invasion (87. 5%) of colorectal cribriform comedo-type adenocarcinoma were significantly higher than that of common-type adenocarcinoma (22. 7%) (P<0. 05). There were no statistical significance of age, sex, and molec-ular genetics of KRAS and mutated BRAF mutation between two groups ( P>0. 05 ) . Conclusions There is a higher clinical stage and poorer prognosis including unique morphological characteristics, highly lymphovascular invasion and lymph node metastasis in pa-tients with colorectal cribriform comedo-type adenocarcinoma.

Objective To establish nursing safety management system in the operating room and explore the effect of clinical application.Methods Toally 350 patients with surgical treatment from January to December 2013 were assigned as the control group,where routine nursing safety management was conducted.Another 350 patients from June 2014 to June 2015 were as the observation group,where nursing safety management system was used in the operating room.The two groups were compared in terms of adverse events,the passing rate of quality and safety.Result As compared with the contrast group,the incidence of adverse events was significantly lower and the passing rate of quality and safety was significantly higher in the observation group (P＜0.05).Conclusion Establishment of nursing safety management system can improve the nursing risk awareness,reduce the incidence of preoperative and postoperative adverse events and improve the level of nursing safety management and the quality of care.

To observe different expression patterns of β-catenin and its clinical significance in colorectal cancer (CRC).Methods A total of 181 cases of CRC tissues and 30 cases of normal colorectal tissue were investigated by immunohistochemistry for the expression of β-catenin.Results The expression rate of β-catenin was 56.9％ (103/181) in CRC,and higher than that in normal colorectal tissue (P ＜ 0.05).The overexpression of nuclear β-catenin was significantly correlated with histological differentiation,lymph node metastasis and Dukes' stage in CRC (P ＜ 0.05),and no relationship with other pathological parameters,such as age,gender and the depth of infiltration.The incomplete membranous expression of β-catenin was significantly correlated with histological differentiation,the depth of infiltration,lymph node metastasis and Dukes' stage in CRC (P ＜ 0.05).The high expression of nuclear β-catenin related to histological differentiation and Dukes' stage in CRC (P ＜ 0.05).In the follow-up data of 82 cases of CRC,the expression of nuclear β-catenin was associated with poor prognosis,and the 5-year survival rate was significantly lower than that of self-control groups (P ＜ 0.05).Conclusion β-catenin plays important roles in colorectal carcinogenesis.Abnormal expression of β-catenin was related to the aggressive progression of CRC and may be helpful for evaluating the prognosis of patients with CRC.β-catenin is expected to become a new target for diagnosis and treatment of CRC in future.

Purpose To investigate the expression and clinical significance of microRNA-21 (miR-21) in patients with prostate cancer (PCa).Methods The human tissue specimens were paraffin-embedded blocks which were collected from the Department of Pathology.miR-21 was detected by in situ hybridization (ISH) in 90 cases of PCa and 69 cases of benign prostatic hyperplasia (BPH).Results The ISH data showed that the positive rate of miR-21 in PCa (71.1％) was significantly higher than that in BPH (24.6％) (P ＜ 0.05),the expression of miR-21 correlated with the Gleason grade (P ＜ 0.05),and no relationship was observed with other clinicpathological features (P ＞ 0.05).Conclusion The miR-21 plays an important role in the pathogenesis of PCa,which can be a new diagnostic target for PCa.

Objective To investigate the expression of inducible co-stimulator (ICOS) on T lymphocytes in peripheral blood from the patients with systemic lupus erythematosus (SLE). Methods Two-color immunofluorescent staining and flow cytometric assay were used to analyze the level of ICOS expression on T lymphocytes in peripheral blood from 33 SLE patients and 16 healthy volunteers. The data of systemic lupus erythematosus diseases activity index (SLEDAI) in SLE patients were also collected. Results The level of ICOS expression on T cells from active SLE group was markedly higher than that in the control group and the inactive SLE group (P 0.05). However, a positive correlation between the expression level of ICOS and the SLEDAI score was found in both active SLE group and inactive SLE group(r = 0.711,P = 0.001?r = 0.561,P = 0.03). Conclusions The expression of ICOS on T cell in peripheral blood from active SLE is increased, which might play a role in the pathogenesis of SLE.

The expression of inducible co-stimulator (ICOS) in peripheral blood T lymphocytes from the patients with systemic lupus erythematosus (SLE) and the role in the pathogenesis of SLE was investigated. By using two-color immunofluorescent staining and flow cytometric assay, the expression levels of ICOS in peripheal blood T lymphocytes from 33 patients with SLE and 16 healthy volunteers were detected. SLE diseases activity index (SLEDAI) of the patients with SLE was used to evaluate the disease activity. The correlation between the ICOS expression and SLEDAI was analyzed among the groups. The results showed that the expression levels of ICOS in T lymphocytes in active SLE group was markedly higher than those in the control and inactive SLE groups (both P< 0.01). There was no significant difference in the expression levels of ICOS between the inactive SLE and the control groups (P>0.05). In active SLE and inactive SLE groups, positive linear correlation was found between the levels of the ICOS expression in T lymphocytes and SLEDAI (r=0. 711, P=0.001; r=0.561, P=0.03). It was suggested that the expression of ICOS in peripheral blood T lymphocytes from the patients with active SLE was up-regulated and and ICOS might be related to the pathogenesis of SLE.
Antigens, Differentiation, T-Lymphocyte/*biosynthesis ; Antigens, Differentiation, T-Lymphocyte/genetics ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/etiology ; Lupus Erythematosus, Systemic/*immunology ; T-Lymphocytes/*immunology

In previous studies, we demonstrated 10 types of lymphocytes in lymph nodes, each exhibited a different fluorescent color by our thioflavine staining method. Among them, 4 types were able to differentiate into plasma cells of the same fluorescent colors. In the present study, different types of lymphocytes were demonstrated in human peripheral blood by their different fluorescent colors after thioflavine staining. The lymphocytes from the venous blood of 50 healthy persons were isolated with Ficoll-Conray solution and E-rosette and EAC-rosette tests and fluorescent staining with thioflavine were performed. Most of the lymphocytes in peripheral blood are small ones with nuclei and cytoplasm showing blue fluorescence and the blue fluorescence of the cytoplasm is paler than that of the nuclei. The nuclei in a part of these lymphocytes have distinct boundaries. The nuclei in another part of these lymphocytes are smaller and with indistinct boundaries and indentation on one side and show dim fluorescence. Other lymphocytes show different fluorescence. Some show blue round nuclei with distinct nuclear membrane, and no color of fluorescence in cytoplasm, but with blue white patches on one side of the nuclei. Some show dark blue nuclei and bright blue cytoplasm and others show orange yellow or orange red nuclei and yellow cytoplasm. In addition, lymphocytes of grayish blue or grayish yellow nuclei and bluish green cytoplasm or lymphocytes of yellowish fluorescence may be seen at times. Very few lymphoeytes of orange red nuclei with nearly no cytoplasm may be seen occasionally.The lymphocytes with blue fluorescence and indentation on one side of nucleus, those with blue nuclei and blue white patches in the cytoplasm as well as those with orange yellow nuclei and yellow cytoplasm can form E-rosettes with sheep erythrocytes. They are T cells. The lymphocytes with distinct boundaries of nucleus, small size and blue fluorescence those with dark blue nuclei and bright blue cytoplasm as well as those with orange red nuclei and yellow cytoplasm can form EAC-rosettes with sheep erythrocytes sensitized by specific antibody and complement. They are B cells. The lymphocytes with blue nuclei and blue white patches may transform into lymphocytes with orange yellow nuclei and yellow cytoplasm under ultra-violet light irradiation. The latter are few in number in the blood but may be progressively increased in number on prolonged observation. They belong to Group Ⅲ of lymphocytes and are mainly located in the paracortical thymus-dependent zone of lymph nodes. The sma ller lymphocytes with blue fluorescence and distinct nuclear boundary may transform into lymphocytes with orange red nuclei and yellow cytoplasm, which are also very few in number in the blood and are also progressively increased in number on prolonged observation. They belong to Group Ⅱ of lymphocytes and constitute the main component of lymph nodules in lymph nodes.

Objective Dieulafoy's lesion is a rare cause of upper gastrointestinal bleeding. The purpose of this study was to recognize the clinical characteristics of gastric Dieulafoy and to identify possible predictive factors of rebleeding. Methods Retrospective study of patients with gastrointestinal bleeding secondary to Dieulafoy's lesion from January 2009 to June 2016. We analyzed the clinical data and endoscopic findings and the correlated with rebleeding risk factors with Dieulafoy's lesion. Results 111 patients were included in the study, 97 (87.4%) patients were male; the most common location of the bleeding lesions were Proximal stomach of 53 cases (47.7%); According to the Forrest type, 46.8% of the cases were arterial (spurting), 52.3% of the cases were arterial (oozing), there were 101 (91.0%) patients treated by endoscopic combined drug therapy. The success rate of Endoscopic hemostatic treatment was 84.2%, endoscopic hemostatic treatment success rate was as follows: single endoscopic, 85.0%; two endoscopic, 84.8%; three endoscopic, 75.0%. The hemostatic treatment success rate of 101 patients with endoscopic combined drug was as follows: Proximal stomach, 83.7%; mid-stomach, 82.1%; and distal stomach, 88.9%. Age (P = 0.002) and blood transfusion (P = 0.004) were risk factors for rebleeding in the study. Blood transfusion was associated with a higher recurrence rate for bleeding (P = 0.018, OR=37.77, 95% CI = 1.86~766.47) for 101 patients with endoscopic in combination with drug. Conclusion Endoscopic therapy is effective for treating Dieulafoy's lesion. The blood transfusion was associated with a high rate of bleeding recurrence. There were no significant differences between the rebleeding and non-rebleeding groups with respect to bleeding location or hemostatic methods.