Objective To investigate the role of tyrosine kinase Src in a murine C.albicans infection model. Methods Observed cell proliferation by alarmarblue assay at 2, 24 and 48 h after Src inhibitor PP2 treatment. Phagocytosis was determined by a fluorometric assay. Cytokine TNF-αand IL-10 production was detected by ELISA. Results The 0~33.3 μmol/L PP2 had no effect on cell proliferation after PP2 treatment for 2 h. When the PP2 treatment extended to 24 or 48 h, PP2 (11.1, 33.3μmol/L) showed significant inhibition on cell proliferation with 78%, 9%, and 54%,13%, respectively. At 48 h after 11.1μmol/L PP2 treatment, the internalization of C.albicans in macrophage is significantly inhibited, contributing to the inhibition of cell proliferation. However, the 11.1 and 33.3μmol/L PP2 significantly inhibited the cytokine TNF-αand IL-10 production during C.albicans infection (P<0.01). Conclusion Src kinase played an important role during C.albicans infection, especially for the cytokine TNF-αand IL-10 production.

Objective To explore the risk factors for gestational diabetes mellitus (GDM ) in advanced maternal age (AMA) at the early stage of pregnancy .Methods A total of 1200 subjects with AMA were enrolled in this study as AMA group from Jun 2016 to Jul 2017 in Beijing Obstetrics and Gynecology Hospital of Capital Medical University .Each group was further divided according to OGTT results :GDM group and NGDM group .The risk factors of GDM were analyzed .Results The prevalence of GDM was 24.17%(290/1200) .The history of type 2 diabetes in first-degree relatives,pre-pregnancy BMI,TG,FPG,FIns,and HOMA-IR were significantly different in the two groups (P＜0.05) .Pearson correlation analysis showed that GDM was positively correlated with the history of type 2 diabetes in first-degree relatives,pre-pregnancy BMI,TG,FPG,FIns and HOMA-IR (r= 0.519,0.413,0.451, 0.625,0.531,0.214,P＜ 0.05) .Multivariate logistic regression analysis showed that history of type 2 diabetes in first-degree relatives,pre-pregnancy BMI,TG,FPG,FIns and HOMA-IR were independent risk factors for GDM (OR=1.635,1.133,2.153,2.418,1.390,1.901,P＜0.05) .Conclusion During early pregnancy in subjects with AMA,TG,FPG,FIns,history of type 2 diabetes in first-degree relatives,pre-pregnancy BMI were independent risk factors for GDM .