This article discussed the necessity and research significance of TCM literature digitization from the aspects of features of TCM literature and connotation and advantages of literature digitization. It introduced the research background of TCM literature digitization by using the examples of domestic and foreign digital libraries and digitized periodicals, expounded the research status of TCM modern and ancient literature digitization, elaborated the research condition of TCM ancient literature digitization, and analyzed the existing problems and provided suggestions.

Adult ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Arthritis, Rheumatoid ; diagnosis ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Spondylitis, Ankylosing ; diagnosis ; drug therapy ; Tripterygium ; chemistry

Acupuncture and moxibustion for treatment of neurogenic bladder dysfunction after spinal cord injuries at home and abroad in recent years is introduced. The acupuncture and moxibustion methods for treatment of this kind of disease are classified and analyzed from body acupuncture, electroacupuncture, acupoint pressure and massage, acupoint-injection, acupoint sticking and so on. Acupuncture and moxibustion are widely applied to treatment of neurogenic bladder dysfunction after spinal cord injuries, with definite therapeutic effect and good safety. The effectiveness of other evaluation indexes of acupuncture and moxibustion for treatment of neurogenic bladder dysfunction after spinal cord injuries needs to be proved by more randomized and controlled trials of high quality with strict design and scientific methods.
Acupuncture Points ; Acupuncture Therapy ; methods ; Humans ; Spinal Cord Injuries ; complications ; therapy ; Urinary Bladder, Neurogenic ; therapy

Aim To investigate the curative effect of rAAV-PR39-ADM,which co-expressed the gene of an-tibacterial peptide (PR39 ) and adrenomedullin (ADM),in a rat cerebral ischemia/reperfusion (I/R) injury.Methods In vitro,Matrigel angiogenesis as-say was made with human umbilical vein endothelial cells.In vivo,the cerebral I/R model was established by the occlusion of the cerebral artery for 2h and then reperfused for 24 h.SD rats were randomly divided in-to sham group,I/R+normal saline group,I/R+null virus (AAV ) group, and IR +rAAV-PR39-ADM group.rAAV-PR39-ADM,saline and null virus were administered through the femoral vein after 24 h of the reperfusion in I/R group.MRI,neurological deficit score,TTC and HE staining were measured respective-ly 1 ,2,3 and 4 weeks after the injection in order to e-valuate the therapeutic efficacy.Results In vitro, rAAV-PR39-ADM group had significant angiogenic effect compared with sham group and null virus group. In vivo,successful I/R model was verified by the ima-ges of MRI.Compared with sham group,the nerve function defect score and the cerebral infarction size in each time nodes were significantly raised in I/R groups (P<0.01).There was no significant difference in in-farct size and nerve function defect score between I/R+normal saline group and I/R +null virus (AAV ) group,and obviously,the IR +rAAV-PR39-ADM group lowered these indexes compared with the other two groups.HE staining showed that the number of neurons,new capillaries vessels of I/R +PR39-ADM group were significantly more than those in group I/R and group I/R +null virus.Conclusion The treat-ment of rAAV-PR39-ADM promotes vascular forma-tion,neuron protection and reduces the infarct size in the model of cerebral ischemia/reperfusion.

This article briefly described the existing problems in talent training of TCM informatics, conducted discussion according to discipline personnel training practice teaching mode, proposed to introduce Arduino open-source hardware into TCM informatics teaching, try to carry out practice exploration for existing classroom and experiment teaching, and proposed relevant suggestions to promote learning interest of students and improve their application ability, with a purpose to further improve the practice teaching mode of TCM informatics, cultivate "TCM+informatics" compound and application talents to promote long-term development of this discipline.

OBJECTIVETo observe the differences in therapeutic effect between penetrating needling on head points and acupuncture of syndrome division in the treatment of vertigo caused by vertebral-basilar arterial blood-supply insufficiency.

METHODSSixty cases of vertigo caused by vertebral-basilar arterial blood-supply insufficiency were divided into 2 groups: a penetrating needling on head points group (group A) and an acupuncture of syndrome division group (group B), 30 cases in each one. In group A, penetrating needling technique was applied from Baihui (GV 20) towards Qianding (GV 21), Shuaigu (GB 8) towards Qubin (GB 7) and Yuzhen (BL 9) towards Tianzhu (BL 10). Electric stimulation was added. In group B, the acupoints were selected according to syndromes. For example, upper disturbance of wind yang: Ganshu (BL 18), Xingjian (LR 2),etc. were selected; upper disturbance of turbid phlegm: Yinlingquan (SP 9), Fenglong (ST 40), etc. were selected; qi and blood deficiency: Baihui (GV 20), Xuehai (SP 10), etc. were selected; liver and kidney yin deficiency: Ganshu (BL 18), Shenshu (BL 23), etc. were selected. Electric stimulation and needling manipulation were conducted on those acupoints. Ten treatments made one session. After continuous 2 sessions of treatment, the efficacy, symptom score and physical sign score were compared between two groups. Transcranial Doppler (TCD) examination was done to observe hemodynamic changes of anterior cerebral artery (ACA), middle cerebral artery (MCA) and posterior cerebral artery (PCA) before and after treatment.

RESULTSThe total effective rate in group A was 96.7% (29/30) that was obviously better than 83.3% (25/30) in group B (P<0.05). The scores of vertigo degree, vertigo frequency, duration and accompanied symptoms after treatment were lower obviously as compared with those before treatment in two groups (all P<0.05). The improvements in group A were much significant (all P<0.05). The highest mean velocity(Vm) of bilateral MCA, ACA and PCA were apparently reduced after treatment in two groups (P< 0.05, P<0.01). The improvements in group A were much more apparently (all P<0.05).

CONCLUSIONThe penetrating needling on head points can effectively relieve vertigo caused by vertebral-basilar arterial blood-supply insufficiency, reduce the attack frequency and improve in its accompanied symptoms. Its clinical efficacy is significantly superior to that in acupuncture of syndrome division group.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Basilar Artery ; physiopathology ; Blood Circulation ; Female ; Head ; blood supply ; Humans ; Male ; Middle Aged ; Vertebral Artery ; physiopathology ; Vertebrobasilar Insufficiency ; complications ; therapy ; Vertigo ; etiology ; physiopathology ; therapy

OBJECTIVETo explore the protective mechanism of penetration therapy with scalp electroacupuncture on Parkinson's disease (PD).

METHODSThirty-six Wistar rats were randomly divided into a normal group, a sham operation group, a model group and a penetration therapy group, 9 rats in each group. The sham operation group was operated by micro-injection with normal saline in the left corpus striatum. The model group and penetration therapy group were generated by micro-injection with 6-hydroxydopamine in the left corpus striatum to prepare rotation model of PD. The penetration therapy group was treated by penetration therapy with scalp electroacupuncture through "Baihui" (GV 20) to "Taiyang" (EX-HN 5), once each day, 6 days for one course, altogether 2 courses, and there was no treatment in the other two groups. (1) Immunohistochemical method was used to test the morphology and count of positive cells of tyrosine hydroxylase (TH). (2) In situ hybridization histochemistry was applied to detect the mRNA expression of brain-derived neurotrophic factor (BDNF).

RESULTS(1) The areal density (AD), numerical density (ND) and integrating optic density (IOD) of the positive neurons of TH in substantia nigra in the penetration therapy group were 0.065 +/- 0.011, 0.014 +/- 0.003 and 0.470 +/- 0.099, respectively, which were higher than 0.039 +/- 0.008, 0.008 +/- 0.002 and 0.266 +/- 0.065 in the model group (all P < 0.05). (2) The AD, ND and IOD of the mRNA expression of BDNF in substantia nigra in the penetration therapy group were 0.100 +/- 0.012, 0.014 +/- 0.003 and 1.158 +/- 0.130, respectively, which were higher than 0.047 +/- 0.012, 0.007 +/- 0.001 and 0.602 +/- 0.108 in the model group (all P < 0.05).

CONCLUSIONThe penetration therapy with scalp electroacupuncture has a better protective effect on dopaminergic neurons in substantia nigra of rats with PD. The protective mechanism is related to the provocation of neural nourishment so as to improve the morphous of dopaminergic neurons and increase the number of dopaminergic neurons.

Animals ; Disease Models, Animal ; Electroacupuncture ; Female ; Gene Expression ; Humans ; Male ; Nerve Growth Factors ; genetics ; metabolism ; Parkinson Disease ; genetics ; metabolism ; therapy ; Rats ; Rats, Wistar ; Scalp ; Substantia Nigra ; metabolism

OBJECTIVETo explore the mechanism of electroacupuncture scalp point-through-point therapy in the treatment of Parkinson's disease.

METHODSThirty-six Wistar rats were randomly divided into a normal group, a sham-operation group, a model group and a point-through-point therapy group. 6-()HDA was injected into left striatum to made lateralization Parkinson's disease rat model. The point-through-point therapy group was treated with electroacupuncture at "Baihui" (GV 20 )-through-"Taiyang" (EX-NH 5), once each day, 6 days constituting one course, for 2 courses, and the rats of other groups were not treated. HE staining method was used for observation of the histo-morphologic changes of the substantia nigra neurons, and RT-PCR for the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) mRNAs.

RESULTSThe expressions of TH mRNA (1.22 +/- 0. 19) and DAT mRNA (0.62-0.11) in the point-through-point therapy group were significantly higher than (0.65 +/- 0.17) and (0.41 +/- 0.08) in the model group, respectively (all P < 0.05). As compared with the model group, the number of neurons in the substantia nigra increased and degeneration of the neurons relieved in the point-through-point therapy group.

CONCLUSIONThe electroacupuncture scalp point-through-point therapy can increase expressions of TH mRNA and DAT mRNA in the substantia nigra in the Parkinson's disease model rat, and promote synthesis and reuptake of dopamine, hence Parkinson's disease is cured.

Acupuncture Points ; Animals ; Disease Models, Animal ; Dopamine Plasma Membrane Transport Proteins ; genetics ; metabolism ; Electroacupuncture ; Female ; Gene Expression ; Humans ; Male ; Parkinson Disease ; genetics ; metabolism ; therapy ; RNA, Messenger ; genetics ; metabolism ; Random Allocation ; Rats ; Rats, Wistar ; Substantia Nigra ; metabolism ; Tyrosine 3-Monooxygenase ; genetics ; metabolism

OBJECTIVETo screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responsing to the aqueous extract from dried powdered rhizomes of Typhonium giganteum (AEoTGE).

METHODThe response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE was explored with the technique of mRNA differential display.

RESULTAfter hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, 1 gene expression was upgrade and 1 gene expression was downgrade induced by AEoTGE.

CONCLUSIONThe research has provided important clues for the molecular mechanism of how hepatocarcinoma cells responseing to T. giganteum.

Araceae ; chemistry ; Carcinoma, Hepatocellular ; genetics ; pathology ; Cell Line, Tumor ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Liver Neoplasms ; genetics ; pathology ; Plants, Medicinal ; chemistry ; RNA, Neoplasm ; genetics ; Rhizome ; chemistry

Objective : To investigate the willingness to receive HIV testing in primary health service institutions (PHSIs) among young students in Wuhan City, so as to provide the evidence for improving the detection of HIV testing among young students. Methods: Fifteen PHSIs were sampled using a stratified random sampling method in 14 districts of Wuhan City, and school students at ages of 15 to 24 years were sampled from each district using a convenience sampling method. Participants' demographics, awareness of AIDS-related knowledge, HIV testing and willingness to receive HIV testing were collected using questionnaires, and factors affecting the willingness to receive HIV testing in PHSIs were identified among school students using a multivariable logistic regression model. Results : A total of 301 questionnaires were allocated, and 299 valid questionnaires were recovered, with an effective recovery rate of 99.34%. The respondents included 143 men (47.83%) and 156 women (52.17%), and had a mean age of (19.36±2.40) years; there were 223 respondents with an educational level of diploma and above (74.58%). The awareness of AIDS-related knowledge was 71.57% among the respondents, and 144 respondents had received AIDS-related health education in PHSIs (48.16%). There were 34 respondents that had received HIV testing (11.37%) and 203 respondents that were willing to receive HIV testing in PHSIs (67.89%). The respondents that were unwilling to receive HIV testing in PHSIs were mainly attributed to considering to be unlikely to get HIV infections (82.29%). Multivariable logistic regression analysis showed that school students who knew AIDS-related knowledge (OR=2.797, 95%CI: 1.583-4.941), knew free HIV counseling and testing services in PHSIs (OR=2.070, 95%CI: 1.123-3.814), and had received AIDS-related health education in PHSIs (OR=2.814, 95%CI: 1.573-5.032) were more willing to receive HIV testing in PHSIs. Conclusions There were 67.89% of school students that were willing to receive HIV testing in PHSIs in Wuhan City, and the willingness to receive HIV testing was correlated with the awareness of risk of HIV infections, and awareness and experience of AIDS control services in PHSIs.