Objective To evaluate the value of serum CA 19-9 levels in predicting resectability of pancreatic cancer. Methods Serum CA 19-9 levels of 52 patients with pancreatic cancer who were possible to be curatively resected according to the imaging were determined; the gold standard was respectability. An receiver operating characteristic (ROC) curve was constructed for the CA 19-9 levels; the point that lies closest to the upper left-hand comer of the graph is chosen as the cut-off point, the sensitivity, specificity,positive and negative predictive values were calculated. Results There were 29 (55.8%) resectable and 23 (44.2%) inoperable cases of pancreatic cancers. The CA19-9 level in resectable patients was (159.6±170.9)U/ml, and it was (944.4 ±773.4) U/ml in inoperable cases. The area under the ROC curve was 0. 918 ( ＞ 0.9, P ＜ 0.01 ), 95% confidence interval were 0.843 ～ 0.992. The CA19-9 level was (353.2) U/ml according to the cut-off point, the sensitivity and specificity was 93.1% and 78.3%, respectively; positive and negative predictive values was 84. 4% and 90. 0% , respectively. Conclusions Preoperative serum CA19-9 level may be a useful marker for further evaluating the respectability of pancreatic cancer who are possible to be resected according to the imaging studies.

Objective To establish the differentiation standard of stasis-heat TCM syndrome. Methods With Delphi statistics method, the expert questionnaire of diagnosis and treatment on stasis-heat TCM syndrome was worked out on the basis of literature research, and sent to more than 30 experts in two rounds. The investigation results and the suggestion of experts were analyzed. Results The recede effective questionnaire amount was 32 and 31 respectively in two rounds. The positive coefficient was 94% and 97% respectively. According to the investigation results, the standard of syndrome differentiation of stasis-heat syndrome was made. The suggestions from experts were about the same. The clinical manifestations of stasis-heat syndrome includes fever, bleeding, pain, abnormal mind, specific manifestations on skin, complexion, tongue and pulse, etc. The main points of syndrome differentiation are deep red tongue, red and blue purple choroides, crimson face, florid bleeding or bleeding with dark purple blood clots, and red purple tongue. Conclusion Differentiation standard of stasis-heat TCM syndrome formulated by Delphi statistics method conform to the thinking of TCM syndrome differentiation. The researchresults gave prominence to the pathogenesis characteristics of stasis-heat TCM syndrome and played a part in gathering expert consensus.

Objective: To investigate the effects of recombinant human erythropoietin (rhEPO) on the expression of heme oxygenase-1(HO-1) mRNA after renal ischemia/reperfusion (IR) in mice. Methods: Ninety male C57BL/6 mice were randomly divided into three groups, namely, the sham operation group (n = 30), renal IR group (n = 30), and rhEPO treatment group (n=30). Mice were sacrificed at 1, 2, 3, 6, 24, and 48 h after renal reperfusion, and the renal function was evaluated by determining blood creatinine. Histological damages were observed using a semi-quantitative histomorphological scoring system from 0 to 4. Cell apoptosis was analyzed by TUNEL staining in each group. HO-1 and IL-6 mRNA expression was examined by real-time PCR. Results: Compared with renal IR group, the expression of HO-1 mRNA was significantly higher in rhEPO treatment group at 3 , 6, and 24 h after reperfusion(P<0.05). The expression of IL-6 mRNA was significantly lower in the rhEPO treatment group at 6, 24, and 48 h after reperfusion(P<0.05). Serum creatinine level in the rhEPO treatment group was significantly lower than that in the renal IR group at 24 h after reperfusion(P<0.05). Compared with the renal IR group, renal histology injury was greatly attenuated by rhEPO in rhEPO treatment group. TUNEL staining analysis indicated that the apoptotic cells in the IR group were significantly more than those in the sham operation group(P<0.05), and those in the rhEPO treatment group was significantly less than those in the IR group(P<0.05). Conclusion: rhEPO can attenuate renal ischemia/reperfusion injury in mice, probably through promoting the renal expression of HO-1 mRNA.

Objective: To develop a compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues. Methods: A staining meth od combining Masson Fontana silver nitrate, Ponceau S, Picric acid and Victoria blue (MFSN-P-PA-VB) was used in the present animal skin experimental. Results: The melanin granule was blac k in the skin melanocyte, collagenic fibers were red, the red blood cells at hemorrhagic focus were yellow, and the elastic fibers were green by this MFSN-P-PA-VB staining method. Conclusion: This compound staining method has multicol or and fine contrast, and can better show melanins, the red blood cells at hemorrhagic focus, and collagenic and elastic fibers.

Objective: To investigate the distribution of neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues. Methods: Rats were decapitated and the pancreatic tissues were harvested, frozen and made into 8 μm serial sections. A combined-staining method (K-R-VB-P-PA method, a combination of Karnovsky-Roots and Victoria blue-Ponceau-Picric acid) was used for staining and the distribution of neural cholinesterase and elastic and collagen fibers was observed. Results: The results showed that the neural cholinesterase was brown, the elastic fibers were cyan, the collagen fibers were light red, and the smooth muscle was yellow in the pancreatic acinar, islet of Langerhans and blood vessels. Conclusion: K-R-VB-P-PA combined staining method can demonstrate the distribution of neural cholinesterase, elastic and collagen fibers and smooth muscle fibers in the pancreatic tissues synchronously.

Lung cancer, a malignant cancer with highest death rate, severely threats the people's health. 80% lung carcinomas are non-small cell lung cancer (NSCLC). Recent studies have found that estrogen system has close relationship with the biological behaviors of NSCLC. Meanwhile, the level of estrogen influences the progression of disease and the prognosis of lung cancer patients. As a result, estrogen pathway has become a hot target in NSCLC treatment. This paper reviews the recearch advances of estrogen system in NSCLC treatment.

Adult ; Aged ; Female ; Herbicides ; poisoning ; Humans ; Male ; Middle Aged ; Paraquat ; poisoning ; Poisoning ; therapy ; Young Adult

Objective:To improve the recognition of bleeding risk in the anticoagulant process by analyzing one case of senior patient with deep vein thrombosis occurred severe bleeding during the anticoagulant therapy.Methods:The possible causes of hemorrhage were analyzed after the brief introduction of medical history,lab test report and treatment process of the patient.Results:The reasons of hemorrhage might be as follows:① the combination of warfarin and dalteparin induced the adverse drug reaction;② albumin decreased during the treatment process resulting in the effect enhancement of warfarin;③ the clearance of warfarin decreased resulting from the low creatinine clearance of the elderly patient.Conclusion:Clinical anticoagulant practice should be more careful in senior patients.The frequency of related laboratory tests should be increased in order to find potential risks timely.

Objective To improve the existing folded grid tent and enhance the efficiency of medical support.Methods The simple pulley and handling dolly were developed,and the skeleton rod was strengthened.Results The improved folded grid tent was easy to carry,transport and handle,and the skeleton rod was solid enough.Conclusion The improved folded grid tent is gifted with an enhanced support efficiency and a longer service time.

Bone metastasis is one of the most common complications of malignant tumors.Cell adhesion molecules(CAMs),including cadherin family,integrin family,selectin family,immunoglobulin superfamily,and hyaladherin,play an important role in bone metastasis of tumor through homotypic and heterotypic adhesive interactions.In this review,molecular and cellular mechanisms underpinning bone metastasis associated with CAMs,the role in a multi-step bone metastatic cascades will be discussed.