Child ; Diagnosis, Differential ; Humans ; Lung Diseases ; diagnosis ; Tuberculosis, Pulmonary ; diagnosis

Objective: The purpose of this study was to establish a human lung adenocarcinoma cell line SPC-A-1 BM with high bone metastatic potential and detect the bone metastasis in immunodeficient NIH-BNX mice by using in vivo radionuclide bone imaging technology. Methods: The human lung adenocarcinoma cell line SPC-A-1 was injected into left ventricle of mice to induce bone metastasis. The metastatic lesions in mice were traced by radionuclide bone imaging agent. The lesion was resected and the isolated tumor cells were cultured in vitro to obtain the bone metastatic human lung adenocarcinoma cells. The first passage of bone metastatic human lung adenocarcinoma cells were injected again into mice via vascular system (artery and vein) or transplanted in lung orthotopically. The cells were repeatedly screened for several times. Results: Chromosome analysis showed that the bone metastatic SPC-A-1BM cells detected by radionuclide bone imaging still kept the properties of human origin after several cycles of screening in vivo and in vitro. The influence of 99m Tc-MDP (111 MBq) on the growth of SPC-A-1BM cells was similar with or a little less than X ray film (40 kV, 2 mA, 4 s). The overall sensitivity, specificity and accuracy of radionuclide bone imaging and X-ray film were 97.6%, 73.3%, 94% and 31.3%, 100%, 43%, respectively. Conclusion: The radionuclide bone imaging offers a practical and convenient method for establishing a human lung adenocarcinoma cell line SPC-A-1BM with high bone metastatic potential and detecting the bone metastasis in immunodeficient BNX mice.

Bronchial Diseases ; diagnosis ; diagnostic imaging ; physiopathology ; therapy ; Bronchioles ; pathology ; physiopathology ; Child ; Child, Preschool ; Diagnosis, Differential ; Female ; Humans ; Infant ; Male ; Respiratory Function Tests ; Retrospective Studies ; Tomography, X-Ray Computed ; Treatment Outcome

Objective The purpose of this study is to assess the prognostic significance of over expression of P53, C-erbB-2 in breast cancer. Methods In 48 patients with breast cancer the expression of P53, C-erbB-2, PCNA and ER was determined by the S-P staining immunohistochemical technique. Results The positive rate of P53, C-erbB-2, PCNA and ER were 54％, 43.7％ 52％, 41.6％ seperateiy. Expression of P53 and C-erbB-2 increased with the class of the tissuess. There is significant difference between grade Ⅰ and Ⅱ , Ⅲ (P＜0.05) as well as between lymph node metastasis (＋) and lymph node metastasis (-) patients (P＜0.05). Conclusion The over expression of P53, C-erbB-2 may be a useful prognostic indicator in breast cancer.

Child ; Enterovirus A, Human ; Enterovirus Infections ; Humans

Child ; Child, Preschool ; Female ; Humans ; Immunoglobulin M ; deficiency ; Male

Child ; Chronic Disease ; Cough ; diagnosis ; etiology ; therapy ; Humans ; Practice Guidelines as Topic

BCG Vaccine ; adverse effects ; Child ; Child, Preschool ; Genetic Diseases, X-Linked ; complications ; Granulomatous Disease, Chronic ; complications ; genetics ; Humans ; Infant ; Infant, Newborn ; Lymphadenitis ; etiology ; Male ; Membrane Glycoproteins ; genetics ; NADPH Oxidase 2 ; NADPH Oxidases ; genetics

A retrospective analysis was carried out in 36 patients with blunt diaphragmatic rupture during March 1991 and March 2006. Twenty-two diagnoses were confirmed preoperatively, and the rest 14were confirmed perioperatively. Three patients underwent surgical treatment after no response to conservative therapy. Thoracotomy was performed in 21 patients, laparotomy in 9, thoracotomy plus laparotomy in 5 and combined thoraco-laparotomy in 1. Most diaphragmatic rupture may be caused by blunt collision and could lead to thoracoabdominal injury. In spite of high mortality rate, the condition is usually under diagnosed. Definite diagnosis and timely operation are important to increase survival rate and reduce mortality.