OBJECTIVETo investigate the correlation between drinking behavior and polymorphism combination of extracellular superoxide dismutase (EC-SOD) and aldehyde dehydrogenase 2 (ALDH2) genes and oral squamous cell carcinoma.

METHODSThe genetic polymorphisms of EC-SOD and ALDH2 were analyzed by polymorphism-polymerase chain reaction technique in peripheral blood leukocytes of 750 oral squamous cell carcinoma cases and 750 non-cancer controls.

RESULTSThe frequencies of EC-SOD (C/G) and ALDH2 variant genotypes were 38.27% and 69.47% in oral squamous cell carcinoma cases and 21.07% and 44.40% in healthy controls, respectively. Statistical tests showed significant difference in the frequencies between the two groups (P < 0.01). The risk of oral squamous cell carcinoma with EC-SOD (C/G) was significantly higher than that of controls (OR = 2.32). Individuals carrying ALDH2 variant genotypes had high risk of oral squamous cell carcinoma (OR = 2.85). Combined analysis of the polymorphisms showed that percentages of EC-SOD (C/G)/ALDH2 variant genotypes in oral squamous cell carcinoma and control groups were 30.67% and 6.80%, respectively (P < 0.01). Individuals carrying EC-SOD (C/G)/ALDH2 variant genotypes had high risk of oral squamous cell carcinoma (OR = 8.13). The drinking rate of the case group was significantly higher than that in the control group (OR = 2.70). Statistical analysis suggested an interaction between drinking and EC-SOD (C/G) and ALDH2 variant genotypes, which increase risk of oral squamous cell carcinoma (OR = 25.00).

CONCLUSIONEC-SOD (C/G) and ALDH2 variant genotypes and drinking are the risk factors in oral squamous cell carcinoma, which could carry out a coordinated attack of oral squamous cell carcinoma.

Aldehyde Dehydrogenase ; Carcinoma, Squamous Cell ; Drinking ; Genotype ; Humans ; Male ; Middle Aged ; Mouth Neoplasms ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Risk Factors ; Superoxide Dismutase

0.05), but the values of ET and TNF in the CJ group were significantly lower than those in the EBD group (P

Objective To investigate the role of nurses in civil cardiac death organ donation work.Methods Cooperating,propagating and promoting in civil cardiac death organ donation; building a bridge between the families of organ donation,donor coordinators,physicians and hospital ethics committees; participating in organ donation medical ethics assessment work; cooperating with the doctors do intend to maintain organ donation,access and preservation; protecting donors remains.Results During the time of July 2011 to November 2013,we successfully completed 52 cases of civil cardiac death organ donation cooperating with doctors,and got 41 liver,which entered the national organ allocation system for distribution.Conclusions The nurses will play a more and more important role in work of civil cardiac death organ donation cooperating with doctors.

Objective To explore the mechanism of spontaneous seizures in adaptor protein complex type 3B knockout mice(AP3M2KO mice).Methods AP3M2KO mice were generated.Seizures and electroencephalogram(EEG)were monitored using video camera and telemetry system.Glutamate and GABA releases were determined using in vivo microdialysis method.Results AP3M2KO mice began to suffer from spontaneous seizures 8 weeks after the birth,but did not show any other behavior abnormality.The onset of ictal discharge over the temporal region was synchronized with seizures.There were no significant differences in basal glutamate and GABA releases in hippocampus between AP3M2KO((0.35±0.08)pmol/20μl and(2.94±1.69)fmol/20μl,respectively)and wild-type mice.However,the 50 mmol/L K+-evoked GABA release was impaired in AP3M2KO mice((63.5±11.8)fmol/20μl vs(209.2±63.7)fmol/20 μl,t=4.405,P＜0.05),whereas no significant difference was found in K+-evoked glutamate release.Conclusions AP3M2KO mice suffer from epileptic seizures similar to the clinical features of human epilepsy.The impairment of inhibitory GABAergic transmission iS involved in the mechanism of spontaneous seizures in AP3M2KO mice.

Objective: To evaluate the false positive rate and false negative rate of the Chinese version of the 12-item General Health Questionnaire (GHQ-12) and the related factors in the epidemiological survey of mental ill-nesses in Zhejiang Province. Method: A total of 15000 subjects were randomly selected from the province-wide using multi-stage stratified cluster randomization. Analyses for this paper were made in the quality control sample,10% of the total 15000 subjects (1510 subjects) in which the Chinese version of Structured Clinical Interview for DSM-Ⅳ Axis Ⅰ Disorders (SCID) was used as a golden criterion, and a cutoff score of the GHQ-12 was set to ≥ 3 to define GHQ-12 cases. Results: Totally 1449 subjects (96.0%) completed both the GHQ-12 and the SCID. Adjusted for sampling effects, the false positive and negative rates of the GHQ-12 were respective 14.6% and7.8%. Adjusted for other considered correlates and sampling effects, the adjusted odds ratios (AORs) of GHQ-12 false positive with living in less economically developed urban areas (urban type Ⅱ), with self-reportedly poor physical health, with having ever sought help because of mental problems and with being currently married/co-habited were respective 2.23 (95% CI:1.24～4.01), 2.36(1.36～4.10), 1.53 (1.10～2.14) and 0.51 (0.30～0.86) while AORs of GHQ-12 false negative with being aged 35～49 year group and living in less econom-ically developed rural areas (rural type Ⅲ) were respective 2.59 (1.18～5.67) and 2.72 (1.21～6.14). Conclusion:Factors related to the GHQ-12 false positive and negative are different. The cutoff scores of the GHQ-12 should be used based on the characteristics of subjects during identifying or screening mental illnesses.

Objective:The major aim of this study is to analyze the point mutation at the codon 54th of MBL in healthy North Huis,and to measure the plasma levels of MBL, and to analyze the association between the mutation frequency and plasma MBL concentrations.Methods:PCR-RFLP was used to detect MBL point mutation.MBL plasma concentrations were measured using MBL Oliger ELISA kit.Results:Frequency of point mutation at the codon 54th of MBL in healthy Huis was 0.15. The plasma MBL concentration was (3.40?2.55)mg/L. There was negative correlation between MBL concentrations and gene mutation frequency in huis(r=-0.67).Conclusion:The relationship between frequency of mutation at codon 54 of MBL gene and the plasma MBL concentrations in healthy Huis is negative correlation.

PurposeTo evaluate the effect of tube current on the pseudo-enhancement of renal cyst by simulating the phantom model of simple renal cyst.Materials and Methods 10% glucose and iodine solution with a certain concentration was used to simulate the renal parenchymal background concentration in plain scan, moderate enhancement and maximum enhancement respectively. The diameters of the cysts were 6 mm, 10 mm and 15 mm, respectively, and the cysts were divided into three groups according to different tube current: 119 mAs (group A), 178 mAs (group B) and 297 mAs (group C) while the tube voltage were all 120 kV. Whether pseudo-enhancement exists in cyst under different conditions was determined using an increase of CT value of 10 HU as the critical value. Results In group A, there was pseudo enhancement at the 240 HU background, and it was most significant with the diameter of 6 mm, which was 21 HU. In group B, pseudo-enhancement occurred in cysts with diameter of both 10 mm and 6 mm under the background of 180 HU and 240 HU, moreover, the biggest difference was 20.4 HU and it occurred in cyst with diameter of 6 mm under the background of 240 HU. In group C, pseudo-enhancement only occurred in cyst with diameter of 6 mm under the condition of 125 HU and 240 HU background concentration. Background concentration (F=17.587, P<0.01) and cyst diameter (F=4.214,P<0.05) had greater impact on cyst pseudo-enhancement, the higher the background concentration and smaller the diameter, more significantly the pseudo-enhancement would occur. With the increase of the tube current, the CT volume dose index increased, and the pseudo enhancement value was smaller, but there was no obvious regularity of pseudo-enhancement occurrence rate in cysts with different background concentration and diameter in each group.Conclusion The increase of tube current cannot completely eliminate cyst pseudo-enhancement. High background concentration and small diameter cyst are important factors in pseudo-enhancement. However, increasing the tube current can reduce the probability of occurrence of pseudo-enhancement to some extent. For those with heavier body weight, it might be necessary to increase the tube current to improve image quality and reduce the occurrence of renal cyst pseudo-enhancement.

OBJECTIVETo investigate the correlation between the combination of smoking and the polymorphisms of cytochrome P450 (CYP) 1A1-Msp I and glutathione S-transferase (GST) T1 genes and oral cancer.

METHODSThe genetic polymorphisms of CYP1A1-Msp I and GSTT1 were detected by polymerase chain reaction (PCR) technique in peripheral blood leukocytes of 300 oral cancer cases and 300 non-cancer controls, and the correlation between smoking, the two metabolic enzymes genetic polymorphisms and oral cancer were analyzed.

RESULTSThe frequencies of CYP1A1-Msp I (m2/m2) and GSTT1(-) were 38.33% and 69.33% in oral cancer cases and 21.00% and 44.33% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups (P<0.01). The risk of oral cancer with CYP1A1-Msp I (m2/m2) was significantly higher than that of controls (OR= 2.34, 95%CI 1.76-4.07). The individuals who carried with GSTT1(-) had a high risk of oral cancer(OR=2.84, 95% CI 1.98-4.54). Combined analysis of the polymorphisms showed that percentage of CYP1A1-Msp I (m2/m2)/GSTT1(-) in oral cancer and control groups was 30.67% and 6.67% respectively (P<0.01). The people who carried with CYP1A1-Msp I (m2/m2)/GSTT1(-) had a high risk of oral cancer(OR=8.27, 95%CI 3.63-11.29). The smoking rate of the case group was significantly higher than that in the control group (OR=2.71, 95%CI 1.31-4.52, P<0.01), and statistic analysis suggested an interaction between smoking and CYP1A1-Msp I (m2/m2)/GSTT1(-) genotypes polymorphisms which increased risk of oral cancer (OR=25.00, 95%CI 11.87-35.64).

CONCLUSIONCYP1A1-Msp I (m2/m2) and GSTT1(-) are the risk factors in oral cancer. Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among CYP1A1-Msp I (m2/m2), GSTT1(-) and smoking on the elevated susceptibility of oral cancer.

Case-Control Studies ; Cytochrome P-450 CYP1A1 ; Genotype ; Glutathione Transferase ; Humans ; Mouth Neoplasms ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Risk Factors ; Smoking

OBJECTIVE: To detect potential mutation in a pedigree affected with autosomal recessive non-syndromic deafness. METHODS: Mutation analysis was carried out by next generation sequencing, and suspected mutations were verified by Sanger sequencing. RESULTS: A heterozygous c.235delC mutation of the GJB2 gene, together with compound heterozygous mutations of the OTOF gene [c.1194T>A (p.D398E) and c.2180A>G (p.N727S)] were detected in the proband. The sister of the proband (also had hearing loss) has carried a heterozygous c.235delC mutation in the GJB2 gene, in addition with a heterozygous c.2180A>G(p.N727S) mutation of the OTOF gene. By Sanger sequencing, a heterozygous IVS1+2T>A mutation was further detected in the non-coding region of the GJB2 gene in both sisters. CONCLUSION The compound heterozygous c.235delC and IVS1+2T>A mutations of the GJB2 gene probably account for the hearing loss in the two sisters, among which IVS1+2T>A is considered as a novel pathogenic mutation of the GJB2 gene.
Connexins ; genetics ; DNA Mutational Analysis ; Female ; Hearing Loss, Sensorineural ; genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Membrane Proteins ; genetics ; Mutation ; Pedigree

Objectives To analyze the characteristics of antigenic genes of clinical Bordetella pertussis strains recently isolated by analyzing the sequence of pertussis toxin S1 subunit(ptxS1) , pertactin (Prn) , fimbriae 2 (Fim2) and fimbriae 3 (Fim3 ) genes of four clinical isolates. Methods The 4 clinical isolates were collected in 2002 in Shijiazhuang of Hebei province. Four strains were isolated from pertussis patient's nasopharyngeal aspirate. ptxS1, Prn, Fim2 and Fim3 genes of these strains were amplified and sequenced. The sequences of those genes were compared with those of the isolates in GenBank and the isoaltes used in the production of pertussis vaccine in China. Results The results of the gene sequencing showed the four clinical isolates belonged to ptxS1 A type, which were different from those in vaccine strains. In addition, three Prn and three Fim'3 variants were observed in the four clinical isolates. Sequence analysis showed that the nucleotide sequence and deduced amino acid sequence of those strains had more than 99% identity with those in vaccine strains. The phylogenetic trees of those genes also showed these strains had a higher level of similarity with other Bordetella pertussis strains. Conclusion The four clinical isolates are different from vaccine strains in four antigenic genes, which laid a foundation for further studies on pertussis epidemiology,quality control and development of pertussis vaccine in China.