Objective To evaluate the consistency of global acne grading system (GAGS) and acne lesion counting. Methods After training 3 dermatologists evaluated 60 acne patients by GAGS and skin lesion counting respectively in randomized order sequentially, and evaluated again 5 days later. Results The kappa-value of GAGS (consistency) was 0.675 among 3 different dermatologists, and 0.847 for the same desmatologist. The kappa-value among the 3 different dermatologists and the same dermatologist for the total lesion count was 0.426 and 0.413 respectively; for pustule count was 0.446 and 0.407 respectively. The kappa-value of total number of the inflammatory lesion counting was 0.429 for the 3 different dermatologists and the kappa-value was

Objective: To study the relationship between the depth and width of cavity design and to analyze the tendency of breaking load in preparations. Methods:Plaster models of mandibular first molar were prepared and magnified by 150%. Cavity on occlusal surface was designed and prepared in a cylinder shape with the depth(mm) of 3.0,4.5 and 6.0,and diameter(mm) of 3.0,4.5 and 6.0,respectively. 8 cavity samples were prepared for each design. The breaking load of the tooth models before and after restoration with self-curing resin was measured by Instron tester.Mechanical model static load test and two variable regression analysis were applied to study the mechanical property of the cavity design. Results:Before restoration, with the increase of depth and diameter, the incidence rate of tooth fracture increased. The width was the main factor. After restoration, the load was undertaken by restoration directly, and could be transfered to tooth and periodontal tissue through restoration uniformly. When the diameter of the cavity increased, the diameter of restoration also increased, and thus decreased the load on local structure of the tooth. With two-regression analysis, the regression equation and regression curve were obtained. Conclusion: The fracture of tooth before and after restoration is related to the diameter and depth of cavity design in cylinder shape. The regression equations of changing tendency of breaking load (Y) in preparation caused by the change of depth(X 1) and diameter(X 2) of cavity obtained by two-regression analysis are presented as follows: Before restoration: Y=1593.317-51.178 X 1-79.489X 2.After restoration:Y=1802.928 -192.461X 1+225.128X 2.

Dietary intake and biochemical parameters of zinc nutritional status in 270 pregnant women and 44 nonpregnant women have been studied and the results were as follows: 1.Dietary zinc intake was about 60% of RDA in both groups.2.The mean plasma Zn concentration declined progressively during gestation; the mean SOD activities and plasma Cu/Zn and alkaline phosphatase activities increased progressively.3.The zinc nutritional status of pregnant women in Beijing was poor. Using plasma Zn concentration less than 10.71 ?mol/l as a low limit, the incidence of zinc deficiency was 6.5%, 20.2% and 25.7% at each of three trimesters respectively.4.Plasma Zn concentration is more sensitive in evalution of nutritional status than hair Zn concentration. Plasma Cu/Zn could reflect zinc nutritional status. The mean SOD activities had similar trend with plasma Zn during pregnancy, and positive correlation with plasma Zn in second trimester (r= 0.2236,P

Objective To explore the changes of Th1/Th2 cytokine in patients with Graves disease(GD) and the regulation effect of Shengmai Injection on its balance.Methods The patients with GD were randomly divided into 2 groups,the Shengmai treated group and the Methimazole treated group,30 cases per group and 30 healthy subjects were enrolled in this study.The serum concentrations of their Th1 cytokine(IFN-?) and Th2 cytokine(IL-10) were assayed by ELISA before and after treatment.Results The serum levels of INF-? and IL-10 in patients with GD were significant higher and the ratio of INF-?/IL-10 were significant lower than healthy subjects(P

Aim To observe the effects of 17?-estradiol(E2)and exercise on the protein expression of peroxisome proliferators activated receptor ?(PPAR?)in bone tissues and bone marrow adipocytes in ovariectomized rats.Methods Forty female Sprague-Dawley rats,aged three months,were randomly divided into the following four groups:sham-operation(Sham),ovariectomized(OVX),ovariectomized plus moderate-intensity treadmill exercise(EX,18 m?minute-1,45 min?day-1,5 uphill,4 times?week-1)and ovariectomized treated with E2(25 ?g?kg-1,3 times?week-1)group.At the end of experiment,the morphological changes of femur and tibia were observed decalcifiedly by HE staining and the protein expression of bone PPAR? was detected by Western blot.Results The number of fat vacuoles and PPAR? protein expression in OVX group was significantly higher than that of other three groups.Conclusion E2 and exercise can benefit the bone morphological structure and these effects might be partly related to the inhibition of both PPAR? protein expression in bone tissues and adipogenic differentiation of bone marrow in OVX rats.

Objective Extrapolate the relationships between the width of circumferential rectal mucosa re-sected and the method of either performing single purse-string or performing double purse-string during per-forming the procedure for prolapse and hemorrhoids (PPH), and to assess the impact of both the level of sta-ple line and the width of rectal mucosa resected on clinical outcomes after stapled hemorrhoidpexy. Meth-ods Twenty-eight adult patients with Ⅲ-Ⅳ hemorrhoids were assigned to two groups: 15 patients using sin-gle pursestring and the remaining 13 patients using double purse-string. The mucosal width of doughtnuts of both groups were measured and the data recorded. The doughtnuts resected were sent to pathology examina-tion after stapled hemorrhoids pexy to identify whether or not the specimens involved the smoth muscles. The demographic complications (bleeding, postoperative pain, urinary retention, fecal urgency) were compared and statistically analysed between the two groups, respectively. Results There were no statistical differ-ences between the two groups in terms of postoperative short-term results and complication rates, as well as the proportion of involving smooth muscle in specimens(P >0.05). However, when compared the width of doughtnuts resected in single-pursestring group was smaller than that in double-parse-string group (P < 0. 01). Conclusion The single or double-purse-string suture in PPH can control the width of resected rectal mucosa.

Objective By compared FAB classification of myelodysplastic syndrome with WHO classification of myelodysplastic syndrome,understanded the proposed WHO new classification of myelodysplastic syndrome as soon as possible.Methods Retraced and analysed 78 cases of myelodysplastic syndrome based on examination of the bone marrow.Restults(1)According to the FAB classification,divided myelodysplastic syndrome into 5 kinds:RA,RARS,RAEB,CMML,RAEB-T.(2)According to the WHO classification,its subtypes were adjusted again,and its concluded:RA,RARS,RCMD,RAEB-Ⅰ,RAEB-Ⅱ,5q-del.(3)There were 9 cases possess chromosome to revise particularly in the chromosome check 28 cases myelodysplastic syndrome. Conclusion WHO classification has clinical guiding significance for early diagnosis,therapy observation and prognosis decision.

Objective Through this meta analysis of controlled clinical trials of the effect of aerobic exercise on the maximal oxygen uptake(VO_2max)in sedentary healthy adults,we attempt to provide evidence for exercise prescription.Methods MEDLINE search for controlled trials that involved in the effect of aerobic exercise on the VO_2max from 1976 to 2008 was conducted.The literatures were selected according to inclusion criteria and exclusion criteria.Review Manager 5.0 software provided a systematic evaluation for calculating VO_2 max Weighted Mean Difference with random effects model.Assessment of the sub-group of aerobic exercise intensity and literature publication bias were conducted.Results(1)Thirty five literatures were included in this review;involving 889 adults met the inclusion criteria.VO_2max Weighted Mean Difference is 3.61ml/kg/min,95%confidence interval 2.96～4.25ml/kg/min,with an increase of 13.2%,comparing with the average VO_2max values(27.26 ml/kg/min)before aerobic exercise.(2)The most effective exercise intensity was 70～80%VO_2max,71～80%HRmax and 61～70% HRR according to the sub.group of aerobic exercise intensity meta analysis.Conclusion VO_2max increased significantly due to participating in aerobic exercise in sedentary healthy adults.The results will provide a theoretical basis for me development of aerobic exercise prescription with appropriate intensity.

BACKGROUND:Osteoporosis is a bone metabolic disease that affects women more than men. Prevention and treatment of osteoporosis is becoming a serious medical problem because of the aging of the population. OBJECTIVE:To explore the effects of lithium chloride treatment on bone microarchitecture and bone marrow stromal cel differentiation of ovariectomized osteoporosis rats. METHODS:After ovariectomy, 28 of 30 healthy female Sprague-Dawley rats, 3 months old, were randomly divided into the folowing three groups: ovariectomizedin vivo group (9 rats), ovariectomizedin vitro group (10 rats), and lithium chloride group (9 rats). At the 11th week postoperatively, rats in the lithium chloride were intragastricaly injected with lithium chloride at a dose of 15 mg/kg, three times per week. After 8 weeks of treatment, the bone microarchitectures of the rat left femur in the ovariectomizedin vivo group and lithium chloride group were detected by micro-CT. The bone marrow mesenchymal stem cels were freshly isolated from the bone marrow of the bilateral femurs and tibia of rats in the ovariectomizedin vitro group. After 24 hours of inoculation, the cels were cultured in lithium chloride and divided into 0 mmol/L (control), 1 mmol/L and 5 mmol/L groups. At 6 and 8 days of culture, the medium was changed and lithium chloride with the corresponding concentrations was added. At 10 days of culture, western blot assay was adopted to detect protein expression of Runx-2, SP7 and PPARγ2. RESULTS AND CONCLUSION:(1) Compared with the ovariectomizedin vivo group, the volume density of trabecular bone, number of trabecular bone, and bone volume fraction in the lithium chloride group were significantly increased and the separation of trabecular bone was significantly decreased. However, no differences were seen in the thickness of trabecular bone and structure model index. (2) Lithium chloride at 1 and 5 mmol/L could increase the protein expression of Sp7 and Runx-2 in bone marrow stromal cels, but decrease the protein expression ofPPARγ2. These results indicate that lithium chloride may improve the microarchitecture of the trabeculr bone in ovariectomized osteoporosis rats through stimulating the osteogenic differentiation of bone marrow stromal cels.

Objective Through this meta analysis of controlled clinical trials of the effect of aerobic exercise on the maximal oxygen uptake(VO2max) in sedentary healthy adults,we attempt to provide evidence for exercise prescription.Methods MEDLINE search for controlled trials that involved in the effect of aerobic exercise on the VO2max from 1976 to 2008 was conducted.The literatures were selected according to inclusion criteria and exclusion criteria.Review Manager 5.0 software provided a systematic evaluation for calculating VO2max Weighted Mean Difference with random effects model.Assessment of the sub-group of aerobic exercise intensity and literature publication bias were conducted.Results(1)Thirty five literatures were included in this review;involving 889 adults met the inclusion criteria.VO2max Weighted Mean Difference is 3.61ml/kg/min,95% confidence interval 2.96 ～ 4.25ml/kg/min,with an increase of 13.2%,comparing with the average VO2max values(27.26 ml/kg/min) before aerobic exercise.(2) The most effective exercise intensity was 70～80%VO2max,71～80% HRmax and 61～70% HRR according to the sub-group of aerobic exercise intensity meta analysis.Conclusion VO2max increased significantly due to participating in aerobic exercise in sedentary healthy adults.The results will provide a theoretical basis for the development of aerobic exercise prescription with appropriate intensity.