Objective:Our purpose was to establish an ideal chronic pressure-afterload heart failure rat model which has the transition from cardiac hypertrophy to heart failure. Methods: Chronic pressure-afterload heart failure rat model was induced by gradually constricting the ascending aorta of young rats. Young rats were randomly divided into 2 groups: the constricted and sham-operated groups. Clinical manifestation, tail-cuff blood pressure, organ weight, and hemodynamic data were observed at various time after operation. Results: The overall survival rate was 87%. Tail-cuff pressure began to increase in 4 weeks after operation. Left ventricular hypertrophy appeared in 12 weeks and heart failure in 5 months. Conclusion:It's a practical and reproducible model of cardiac hypertrophy that progresses to chronic heart failure.

With the development of neuroimaging the detection rate of basilar artery tortuosity is significantly high.The occurrence and development processes of basilar artery tortuosity are affected by age,basilar artery length,vertebral artery dominance,and other factors.Basilar artery tortuosity may influence the blood supply of posterior circulation and cause brainstem and cranial nerve pressure leading to clinical symptoms.

Objective To investigate the association between norepinephrine transporter (NET)gene polymorphism and decision-making processing in patients with cerebral infarction.Methods A total of 145 patients with cerebral infarction and 188 normal controls were enrolled in our study.In all subjects,the polymerase chain reaction (PCR) for NET-T182C/G1287A polymorphism assay,gel electrophoresis,image analysis and enzymatic reaction,gene sequencing methods were used.The relationships of NET T182C/G1287A genotypes and alleles with decision-making processing were analyzed in patients with cerebral infarction.All participants completed six kinds of choice situational problems.Results There were significant differences in genotype and allele frequencies of T182C and G1287A polymorphism in NET between the patients with cerebral infarction and control group(for NET-T182C:genotype,x2 =4.437,P=0.049,allele frequency,x2=4.363,P=0.037,OR=0.625,95％CI:0.436-0.895;for NET-G1287A:genotype,x2=8.435,P=0.038,allele frequency,x2=2.765,P=0.036,OR=1.520,95％CI:1.053-2.193).The cerebral infarction patients with three NET-T182C genotypes and T/C alleles all completed six choice scenarios,and the scheme selection probability had no significant difference (all P＞0.05).In high-risk and no-risk loss situation (scenario 4),the scheme selection probability had significant difference in cerebral infarction patients with NET-G1287 A genotypes and G/A alleles (P＜0.05 and 0.05,OR=1.657,95％CI:1.149-2.390),and the patients with GG genotype tended to choose high-risk loss scheme,and the probability was obviously lower than that patients with other two genotypes,the patients with G allele tended to choose high-risk loss scheme,and the probability was obviously lower than that in patients with A allele (all P＜0.05).In other five choice scenarios,the scheme selection probability had no significant difference between the patients (all P ＞0.05).Conclusions NET-G1287A polymorphism may be associated with decision-making processing in patients with cerebral infarction.In the high-risk and no-risk loss condition,patients with GG genotype and G allele have more loss risk aversion.

Basilar artery hypoplasia (BAH) has been paid less attention to in the past.However,the increased detection rate makes the clinical significance of BAH to be gradually recognized.BAH have interaction with vascular risk factors,and this may promote the occurrence of ischemic events in posterior circulation.Brain evoked potential can be used as an important means for early detection of this pathophysiological events.The depth study of pathological significance of BAH helps to deepen the understanding of the mechanisms of posterior circulation ischemic events.

Objective To investigate the clinical risk factors of vertebral artery origin tortuosity and its impact on vascular vertigo. Methods Totally 206 inpatients in Department of Neurology were divided into tortuous group (n=137) and non-tortuous group (n=69).Clinical risk factors of cerebral vascular disease,vertigo scales and days of treatment relieve were recorded.Total cholesterol (CH),low density lipoprotein cholesterol (LDL-C),homocysteic acid (Hcy) and high sensitivity Creactive protein (hsCRP) were detected.Cervical computer tomography angiography (CTA) and contrast enhancement magnetic resonance angiography (CEMRA) were completed.The risk factors of vertebral artery origin tortuosity were analyzed with multiple logistic regression analysis. Results Vertebral artery dominance was much more in tortuous group than non-tortuous group(x2 =2.496,P＜0.01).Vertigo scales from 1 to 5 appeared in 17 cases,36 cases,79 cases,4 cases,1 case,respectively,and days of treatment relieve were 1-10 d in tortuous group,hut the corresponding data was 18 cases,35 cases,10 cases,3 cases,1 case and 1-6 d in non-tortuous group with statistical significance between the two groups(t=2.014,2.849,P＜0.01).The distinctions were found in age more than 65 years,hypertensive disease,high LDL-C,diabetes mellitus,smoking,stroke history,vertebral artery dominance and cervical spondylosis between tortuous group and non-tortuous group(x2=7.498,5.182,3.724,10.46,6.883,2.748,4.496,8.265,all P＜0.05).Age more than 65 years (95％CI:5.36-18.23,OR=4.84),hypertensive disease(95 ％ CI:2.79-16.45,OR=3.21),vertebral artery dominance(95％ CI:3.25-13.49,OR =5.48) and cervical spondylosis (95％ CI:4.38-21.28,OR=3.57) were high clinical risk factors. Conclusions Patients with vertebral artery origin tortuosity present with higher vertigo scales and longer days of treatment relieve.Age more than 65 years,hypertensive disease,vertebral artery dominance and cervical spondylosis are clinical risk factors for vertebral artery origin tortuosity.

Objective To assess the clinical value of transvaginal ultrasonic hysterography in diagnoio of intrauterine diseases. Methods Two-hundred and ninety-eight patients with transvaginal ultrasonographically diagnosed intrauterine diseases were enrolled in this study. All the patients underwent selective operation of ultrasonic hysterography and hysteroscopy. The results before and after ultrasonic hysterography were compared with those of hysteroscopy and pathology. Results Among 298 patients, 221 were diagnosed pathologically, while 198 were diagnosed with ultrasonic hysterography and 206 with hysteroscopy. The diagnostic coincidence of transvaginal ultrasonic hysterography, transvaginal ultrasound and hysteroscopy was 89.59%, 74.16% and 93.21%, respectively. Conclusion The accuracy of ultrasonic hysterography and hysteroscopy in diagnosis of intrauterine diseases is in coincidence. Ultrasonic hysterography is simple and convenient, worthy of clinical application.in gynecology.

Objective To investigate the correlation between expression levels of serum cytokines IL-2,IL-6, IL-8, IL-10, TNF-α and IFN-γ in patients with diffuse large B-cell lymphoma(DLBCL) and chemotherapy resistance. Methods 30 cases of DLBCL patients with chemotherapy resistant, 30 cases of DLBCL patients with chemotherapy sensitive and 20 cases of healthy individuals as normal control group were enrolled. The levels of serum cytokines IL-2, IL-6, IL-8, IL-10, TNF-α and IFN-γbefore, during and after treatment in both DLBCL groups and normal control group were detected by ELISA assay. Results The expression level of serum IL-6 and IL-10 before treatment in DLBCL patients with chemotherapy resistance was significantly higher than that in DLBCL patients with chemotherapy sensitive and normal control group (P ＜ 0.05), however,that after treatment in DLBCL patients with chemotherapy resistance was significantly lower than that before treatment (P = 0.02, P = 0.015). The level of serum IL-6 and IL-10 in patients with DLBCL recurrence into drug resistance was higher than that during of remission (P = 0.004, P ＜0.001). Before treatment, the expression level of serum IL-6 in patients with Ⅲ-Ⅳ stage in chemotherapy resistant group was significantly higher than that in ones with Ⅰ - Ⅱ stage(P ＜0.05). Levels of IL-2, IL-8, TNF-α and IFN-γin chemotherapy resistant group, chemotherapy sensitive group and the normal control group were no differences (P ＞0.05).Conclusion The expression levels of the serum IL-6 and IL-10 were closely correlated with the chemotherapy efficacy of DLBCL, they may be involved in drug resistance of DLBCL.

OBJECTIVE:To provide reference for the rational clinical prophylactic use of antibiotics. METHODS:The cases in whom the clinical prophylactic use of antibiotics between 2006 and 2007 in our hospital showed failure in efficacy were monitored to analyze the cause of failure,meanwhile the clinical samples were sent for culture,isolation,identification and drug susceptibility tests as well as drug resistance analysis. RESULTS:Cephalosporins,Quinolones,Penicillins,Cefhalosporins + enzyme inhibitor were more commonly used antibiotics in our hospital,with drug resistance rates at 57.21%,65.14%,68.63%,and 18.81% respectively. Among the total 240 cases monitored,the prophylactic use of antibiotics totaled 877 times,of which,388 (44.24%) were non-indicated drug use,459 (52.34%) showed drug resistance,286 (32.61%) involved improper drug choice,and 42 (17.50%) showed dual infection. CONCLUSION:The clinical prophylactic use of antibiotics in our hospital is far from perfect; therefore,it is urgent to tighten control on the standard prophylactic use of antibiotics.

Objective To study the expression and clinical significance of ARHGAP4 in colorectal cancer Method Real?time PCR,Western blot and immunocytochemistry were used to detect the expression of ARHGAP4 in colorectal cancer tissues and cell lines ,and the correlation between its expression and clinical features of patients was analyzed Results ARHGAP4 is overexpressed in colorectal cancer tissues and cell lines and its overexpression is correlated with T stage, N stage, clinical stage, and metastasis. Conclusion ARHGAP4 may promote the progression of colorectal cancer ,and have the potential to be a novel prognosis marker.

Purpose To observe the histopathologic changes of acquired immure deficiency syndrome (AIDS) in a Chinese Rhesus monkeys model and the pathogenesis that initiated the changes.Methods Chinese Rhesus monkeys were sacrificed after being inoculated SIVmac239 by Ⅳ(n=2)for four months.Autopsy was carried out by pathologic routine method.The lymph nodes, heart, liver, spleen, lung, kidney, digestive tract and other tissues were selected, the tissues fixed with 10% neutral formalin, and the pathologic sections were prepared by HE staining and immunohistochemical staining and special staining after paraffin imbedding.Results The main histopathological changes appeared in the immune system in different organs. The lymph nodes began to display the complex changes in a short period of time infected by the virus, including proliferation of lymphoid follicles, atrophy, or both; some lymphoid follicles of lymph nodes had few lymphocytes, with fibrous hyperplasia and immune complex (IC) deposition, displaying a burning down phenomenon.Splenomegaly and blood vessel and its endothelial cell proliferation in splenic corpuscles were noted with the immune complex deposition. Other parts of the mucosa-associated lymphoid tissue had different degrees of hyperplasia, or atrophy.Conclusion Histopathologic changes in Chinese Rhesus monkeys infected by SIVmac239 strain are very similar to human AIDS, which suggests that the model is a useful tool for the prevention and treatment study of AIDS.