Objective:To explore the clinical therapeutic effect of simvastatin combined cyclic adenosine monophos-phate on coronary heart disease (CHD)with chronic heart failure (CHF)and levels of serum-related factors.Meth-ods:A total of 78 CHD patients with CHF hospitalized in our hospital from Jan 2011 to Jan 2012 were selected. They were randomly and equally divided into simvastatin group and combined treatment group using number table, both groups received routine treatment,simvastatin group received simvastatin in addition, while combined treat-ment group received cyclic adenosine monophosphate based on simvastatin group.Therapeutic effect,changes of cardiac function indexes and serum factor levels were compared between two groups before and after treatment.Re-sults:The total effective rate of combined treatment group (92.31%)was significantly higher than that of simvasta-tin group (71.79%),P<0.05;compared with before treatment and simvastatin after treatment,there were signifi-cant rise in left ventricular ejection fraction [LVEF,(33.54±3.34)%,(43.41±3.23)% vs.(55.21±3.45)%] and transmitral early/late diastolic peak flow velocity [E/A,(0.63±0.11),(0.70±0.15)vs.(1.01±0.21)],and significant reductions in levels of brain natriuretic peptide [BNP,(536.74±21.41)ng/ml,(117.23±11.57)ng/ml vs.(78.20±10.92)ng/ml]and C reactive protein [CRP,(24.00±2.34)mg/L,(17.01±1.09)mg/L vs.(8.28± 0.81)mg/L]in combined treatment group,P<0.05~<0.01.Conclusion:Simvastatin combined cyclic adenosine monophosphate possess significant therapeutic effect on chronic heart failure of coronary heart disease,it can signif-icantly improve cardiac function and serum factor levels in CHD patients with chronic heart failure,and possess good clinical application value.

OBJECTIVE：To study the effects of Gushikangfuwan on osteoporosis caused by retinoic acid in rats. METHODS:The osteoporosis was induced by retinoic acid(70mg.kg-1,ig)daily in rats for two weeks. The pharmacologic effects of Gushikangfuvan was observed. RESULTS: Compared with the retinoic acid group Gushikangfuwan (6g.kg-1)enhanced the relative bone volume,average bone trabecula and bone Lacuna length in rats.Gushikangfuwan (3g.kg-1,6g.kg-1) also enhanced the content of calcium and phosphorus of thigh bone. CONCLUSIONS：Gushikangfuwan may ameliorate the bone quality and enhance the bone density in rats.

目的分析血常规检测中常见的误差来源,便于建立室内质量保证措施,确保结果准确。方法用全自动血球仪对我院226例就诊患者的血常规标本进行集中检测,对其血常规检测中常见的一些问题进行分析,包括方法学、仪器、试剂、标本采集、外部环境以及操作过程等综合分析。结果针对引起结果偏差的原因,制定相应的控制措施,确保每一份标本检测结果在质量控制范围内,减少非疾病因素对血常规检测结果的准确性的影响。结论针对血常规检测过程中经常出现的问题进行分析,加强标本分析前、中、后的质量控制,影响结果的一些因素是可以防范的。

OBJECTIVETo observe the effects on post-stroke depression treated with acupuncture at the acu points based on ziwuliuzhu (the midnight-noon ebb-flow theory).

METHODSNinety-three patients were randomized into a comprehensive group, a ziwuliuzhu group and a prozac group, 31 cases in each group. In the ziwuliuzhu group, acupuncture was applied to the acupoints based on ziwuliuzhu, once a day, 5 times in a week. In the prozac group, fluoxertine hydrochloride (prozac) was prescribed for oral administration, once a day, 20 mg each time. In the comprehensive group, acupuncture based on ziwuliuzhu combined with the oral administration of prozac were adopted and the treatment frequency was the same as the ziwuliuzhu group and the prozac group. The 4-week treatment was taken as one session in the three groups and 6 sessions were required totally. The clinical efficacy and the Hamilton depression rating scale (HAMD) score and the adverse reaction were compared among the 3 groups.

RESULTSThe total effective rate was 96.8% (30/31) in the comprehensive group, better than 83.9% (26/31) in the ziwuliuzhu group and 80. 6% (25/31) in the prozac group (both P<0.05). In 4, 12 and 24 weeks of treatment, HAMD score was lower significantly than that before the treatment in the three groups (all P<0.05), and the score in the comprehensive group at each time point after treatment was lower than the other two groups (all P<0.05). In 4 and 12 weeks of treatment, there were not different significantly between the ziwuliuzhu group and the prozac group (both P>0.05). In 24 weeks of treatment, HAMD score in the ziwuliuzhu group was lower than that in the prozac group (P<0.05). For the adverse reaction, there were 2 cases in the comprehensive group, 6 cases in the prozac group and 0 case in the ziwzuliuzhu group.

CONCLUSIONThe comprehensive therapy of acupuncture at the acupoints based on ziwuliuzhu and oral administration of prozac is superior to either the simple oral administration of prozac or the ziwuliuzhu acu-puncture in terms of clinical efficacy and the improvements in depressive state. Regarding the clinical efficacy, the impact of simple ziwuliuzhu acupuncture is not different significantly as compared with the simple oral administration of prozac, but it is better than the simple oral administration of prozac in terms of the long-term HAMD score and safety.

Acupuncture Points ; Acupuncture Therapy ; Aged ; Aged, 80 and over ; Depression ; etiology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Stroke ; complications ; Treatment Outcome

Objective Comparing the serum fingerprint of four kinds of extract solution from Banxia Baizhu Tianma Decoction.Methods Taking rabbits as experiment animals,collecting blood,and separating serum after drenching semi-bionic enzyme extracting(SBEE) solution.HPLC was used to detect the fingerprints of medicated serum at different blood-collecting time in order to optimize the best blood collecting time,then the serum fingerprints of four kinds of solution collected were compared.Results HPLC showed that when blood collecting time was 2 h,the number and total area of chromatographic peak had the maximum value;SBEE Solution had the the maximum number and total area of chromatographic peak in the four kinds of solution at the best blood collecting time.Conclusion The component content in SBEE medicated serum is higher than that by other method.

Objective To study the anti-dermatophytic effect of chitosan-acetate solution and to determine the antifungal MIC. Methods To determine the antifungal MIC of chitosan and acetic acid by agar dilution method,respectively. Results Both antifungi MIC of chitosan to Trichophyton rubrum and Trichophyton tonsurans were 2500mg?L~ -1 ,and to Trichophyton mentagrophytes,Microsporum gypseum,C. albicans were 5000mg?L~ -1;while the antifungi MIC of acetic acid to Trichophyton rubrum and Trichophyton tonsurans were 630mg?L~ -1, to Trichophyton mentagrophytes,Microsporum gypseum,Candida albicans were 1260mg?L~ -1. Conclusion Chitosan and acetic acid show inhibitory effect on the growth of dermatophytes.

0.05),but the level of P- selectin in treatment group were obviously decreased compared with control group after the seventh and fourteenth day(P

Objective To find out the iodine content in drinking water in Yi County,and to provide a scientific basis for prevention of iodine deficiency disorders.Methods An administrative village was taken as a unit in Yi County,Hebei Province.One drinking water sample was randomly selected from each village with centralized water supply.For villages with decentralized water supply,five drinking water samples were randomly selected according to directions (east,west,south,north and center) if there were more than five water sources in the village ; all the water source samples were taken if there were less than five water sources.Water iodine content of all of the water samples was tested; geomorphological feature,water type and well depth were surveyed.Water iodine content was determined through arsenic cerium catalytic spectrophotometric method.Results The range of water iodine content was 0.00-18.39 μg/L,and water iodine median was 2.08 μg/L in 1 024 water samples in the county.Water samples of 490,505 and 29 copies were collected in the hills,plains and mountains,and water iodine median was 1.74,2.26,3.15 μg/L,respectively.Concerning water iodine medians in drinking water,mountains were less than hills and plains(x2 =37.36,34.25,all P＜ 0.01),hills were less than (x2=15.27,P ＜ 0.01).Water samples of 742,228,54 copies were collected,respectively,in household well water,tap water and mountain spring water,and water iodine median was 2.08,2.24 and 0.73 μg/L,respectively.There was no significant difference between the water iodine median in tap water and household well water (x2 =2.97,P ＞ 0.05),and the iodine in mountain spring water was less than that of tap water and household well water (x2 =38.23,43.82,P ＜ 0.01).Water samples of 583 and 441 copies were collected,respectively,in the well depth ＜ 40 m and well depth ≥40 m,and water iodine median was 1.81 and 2.24 μg/L,respectively,and there was no significant difference between the water iodine median in the well depth ＜ 40 m and well depth ≥40 m(x2 =2.32,P ＞ 0.05).Conclusions Residents iodine content of drinking water in Yi County is lower; the natural environment is seriously iodine deficiency,and iodine supplementation should be strengthened.

Objective To investigate the correlation of T cell subgroups and natural killer (NK) cell's activity level of peripheral blood of the patients with refractory lymphoma. Methods Flow cytometry was applied to detect T cell subgroups' level and NK cell's activity of peripheral blood in 60 early cure lymphoma patients with chemotherapy before and 20 normal controls , after chemotherapy follow-up they were divided into 30 cases of difficult cure group and 30 cases of effective group. Results Compared with the normal controls, CD+4, CD+4/CD+8 and NK cell in lymphoma patients with chemotherapy before decreased (30.17±8.63 vs 46.52±1.39, t =12.218, P ＜0.05; 0.86±0.45 vs 1.64±0.05, t =11.225, P ＜0.05; 12.39±7.08 vs 19.29±0.84,t =6.365, P＜0.05), while CD+3 and CD+8 cell increased (76.14±10.71 vs 70.48±1.44, t =-3.439, P＜0.05;40.28±14.03 vs 28.35±0.73, t =-5.625, P ＜0.05). Compared with effective group, CD+4 CD+4/CD+8 and NK cell in difficult response group with chemotherapy before decreased (27.70±7.81 vs 33.13±8.82, t =2.163, P =0.036;0.67±0.27 vs 1.10±0.52, t =3.272, P =0.003; 9.87±6.60 vs 15.40±6.58, t =2.771, P =0.008), while CD+3 and CD+8 cell increased (79.67±8.18 vs 71.91±12.00, t =-2.540, P =0.015; 44.70±13.99 vs 34.98±12.41, t =-2.416,P =0.020). Conclusion The detection of T cell subgroups' level and NK cell' s activity in early lymphoma patients before chemotherapy may play a role to diagnose and predict the outcome of refractory lymphoma patients.

Objective With density in healthy people pharyngeal flora of the reference[1],study the respiratory tract infection and the treatment process of the change of density and pharyngeal flora infection treatment of correlation analysis,by using the density of the pharyngeal flora changes to evaluate the treatment of patients with respiratory tract infection and progno-sis.Methods Collected 102 cases of patients with upper respiratory tract infection and 219 cases of lower respiratory tract infection in patients with pharyngeal swab for bacterial culture,study clinical symptom change with the change of pharyngeal bacteria density of correlation.Results 62 cases of upper respiratory tract infection and the treatment group patients with pharyngeal flora normal no treatment group on the symptoms improved and recovered,no statistical difference (P >0.05), and 60 patients with upper respiratory tract infection and pharyngeal abnormal bacterium group the treatment group and treatment group in pharyngeal flora and CPIS score was statistically difference (P <0.05).For 219 patients with lower re-spiratory tract,including 121 cases of bacterial pneumonia,74 cases of severe pneumonia,and 24 cases of patients with lung abscess treatment observation,found that when back to normal pharyngeal flora,the accuracy of clinical pathological changes were 92.6%,86.5% and 87.5%,respectively.Conclmion Pharyngeal bacteria density between healthy people maintained a certain bacteria species and the number of stable,but when respiratory infections bacteria can disorders with the application of broad-spectrum antibiotics can lead to serious result in pharyngeal dysbacteriosis should be combined with pharyngeal bacteria in respiratory anti-infection treatment density changes to evaluate the clinical treatment,can reduce the number of days with antibiotics,patients with avoid induce drug-resistant bacteria and respiratory dysbacteriosis.