1.PREPARATION AND APPLICATION OF HUMAN PRP SYNTHYETIC PEPTIDE
Songyan LIU ; Shihe LIN ; Shuling LIU
Chinese Journal of Zoonoses 2000;(4):15-17
Aim In order to improver the diagnostic rate of Prion disease, solve the lacking sources of nature PrP antigen. ,synthesize the human PrP peptide, prepare the antibody to PrP peptide further. Method A synthetic peptide with the sequence identical to the 15 residues of human PrP, as described by Kretzschmar[1], was synthesized by the solid-phase method. The synthetic peptide was coupled to bovine serum albumin(BSA)by the method of EDCI〔2〕. The polypeptide combined with BSA was used as antigen to immunize the rabbit and detected by immunomethod. Result The PrP polypeptide combined with BSA obtained immunogenicity and anti-PrP synthetic peptide antiserum was successfully obtained. Conclusion The preparation and application of human PrP synthetic peptide can substitute for nature PrP antigen partly. It has laid a foundation for further preparation of monoclonal antibody to PrP and the study of Prion disease.
2.Parasite pollution in aquatic products marketed in Fujian Province
Chenxin LIN ; Shihan LIN ; Weiwei CHEN ; Shuling HUANG ; Dianwei JIANG
Chinese Journal of Zoonoses 2017;33(6):564-568
We studied the status of parasite pollution in fish and shrimps in Fujian Province,and provided basis for prevention and control of parasite pollution and food safety in aquatic products.Stratified random sampling method was used,and Fujian Province was divide into Eastern,Southern,Western,Northern and Central five regions of Fujian province.Based on the data collected from the five regions between 2012 and 2016,digestion and compression methods were conducted to detect the levels of parasite metacercariae and larvae in both freshwater and marine products.Results showed that the total parasitoid infection rate was 5.15% (130/2 524).The infection rate of trematode metacercariae and nematode larvae were 3.72% (94/2524) and 1.43% (36/2 524),respectively.Twenty-eight marine aquatic species were investigated and the infection rate was 17.25 % (88/510),in the form of Anisakis infection.The parasite infection rates in the five regions were 10.38% (27/260) in Mindong,5.84% (27/462) inMinnan,4.63% (30/648) in Minxi,4.64% (29/625) in Minbei and 9.91% (103/1 039) in Minzhong.The freshwater products in Fujian Province have been polluted by parasites and are area-depended.The infection rate of marine aquatic products is kept in a high level.Fujian Province should strengthen the food safety and health publicity,take effective prevention and control strategies,and use early warning mechanisms to insure the food safety in province.
3.Curative effects of Jianpijiedutang combined with Kangfuxin Liquid in the treatment of recurrent oral ulcer
Tingting WANG ; Jinghui GU ; Lin SHEN ; Shuling YU
Journal of Practical Stomatology 2017;33(3):385-387
Objective:To investigate the effects of Jianpijiedutang combined with Kangfuxin Liquid in the treatment of recurrent oral ulcer(ROU).Methods:80 patients with ROU were divided into 2 groups(n=40).The patients in control group were treated with Kangfuxin Liquid local spray,those in the test group were treated with Jianpijiedutang combined with Kangfuxin Liquid spray.The patients were followed up for 24 weeks.The effects were evaluated according to the trial standard of Chinese Stomatological Association.Results:The interval of ROU increased and ulcer number decreased in the 2 groups during the observation period,the test group showed more effective outcome than the control group(P<0.05).In the treatment group ROU interval increased more than in the control group(P<0.01),and ROU ulcer number decreased more(P<0.01).Conclusion:The Jianpijiedutang combined with Kangfuxin Liquid spray is more effective than the latter used only.
4.Influence of nursing intervention on family care of senile cerebrovascular disease
Shuling YU ; Wei LIN ; Hongxia LI ; Xiuju WANG ; Xiaomei YANG ; Shuyun NI
Chinese Journal of Practical Nursing 2014;30(3):5-7
Objective To investigate the service requirements and health and safety problems in home care among senile cerebrovascular disease patients above 65 years old and provide the direction and basis for the home care.Methods 78 cases of elderly patients with cerebrovascular sequela 2 years after discharge were selected as the research objects.One-on-one interviews with patients and their families,the self-made questionnaire were sent out,the patients' service item requirements and the main health and safety problems were reorganized,then the corresponding nursing intervention was implemented.One year later,patients were revisited and investigated again.Results In elderly patients,there were many health and safety issues in home care,their nursing service demand was high.After giving corresponding nursing intervention of health guidance,the incidence of aspiration,fall,falling out of bed,bedsore,sleep disorders,urinary tract infection was effectively reduced.Conclusions The mode of family care should be established on the basis of demand of home care by elderly cerebrovascular disease patients,which conforms to the ac-tual situation of our country,in order to improve patients' self-care ability and life quality.
5.EXPRESSION OF N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT-1 IN EPENDYMAL CELLS OF THE THIRD VENTRICLE OF RAT
Lin ZHANG ; Nuo YANG ; Shuling WANG ; Yan NAN ; Enhua YU ; Li SHEN
Acta Anatomica Sinica 2000;31(2):174-176,插图第16页
Objective To explore the expression of N-methyl-D-aspartate receptor subunit-1 (NMDAR1) in the ependymal cells of the third ventricle of rat. Methods The immunohistochemistry technique was used. Results (1)NMDAR1-immunoreaction (NMDAR1-IR) was strongly expressed in the ependymal cells of the third ventricle; (2)There was no significant sex difference in morphology and distribution of the NMDAR1-IR ependymal cells between male and female. Conclusion The present investigation provided the morphological evidence supporting that glutamate of CSF might regulate ependymal cells via NMDAR.
6.Nephrectomy and removal of inferior vena cava tumor thrombus under profound hypothermia and arrested circulation
Yousheng YAO ; Jian HUANG ; Hai HUANG ; Yanqi YANG ; Shuling PENG ; Kewei XU ; Zhenghui GUO ; Tianxin LIN ; Chun JIANG ; Jinli HAN
Chinese Journal of Urology 2008;29(5):300-302
Objective To study the feasibility and safety of performing nephrectomy together with the removal of complicated inferior vena cava tumor thrombus under profound hypothermia and arrested circulation. Methods After made the median thoraco-abdominal incision, the exploration of the abdominal organs was done. The right kidney, inferior vena cava and renal pedicle were well exposed then. After the whole body heparinization, cannulas were put into ascending aorta, superior vena cava, aortic root and right superior pulmonary vein. The body temperature was reduced to 20℃ with cardiopulmonary bypass unit and the extracorporeal circulation was stopped then. Cut open the inferior vena cava at vena renalis dextra ingress and the F16 urinary catheter was inserted into atrum dextra through inferior vena cava and inflated. The tumor thrombus was pulled out and the right kidney was removed. The inferior vena cava incision was sutured to close and the extracorporeal circulation was resumed and patient was re-warmed.Results The operation time was 330 min and the extracorporeal circulation time was 90 min, while the profound hypothermia with circulatory arrest time was 20 min. The estimated blood loss during operation was 400 ml and 6 unit red cells and 600 ml blood plasm were transfused. The patient was awaked 2.5 h after the operation, food intake resumed 4 days after operation and the patient was discharged on day 10 post-operatively. After 6 months'follow-up, there were no local recurrence and metastasis occurred. Conclusion The technique of profound hypothermia and circulation arrest could improve the safety and efficacy in the treatment of renal cell carcinoma with suprahepatic (level Ⅲ) caval tumor thrombus.
7.The nursing intervention effects on fallopian tube obstructive infertility of combining traditional Chinese and western medicine treatment
Xin KE ; Shuling LIN ; Yanna CHEN
China Modern Doctor 2014;(24):103-105
Objective To discuss the application of fallopian tube obstructive infertility combine traditional Chinese and western medicine treatment the curative effect and nursing. Methods Selected 100 cases of infertility patients from September 2010 to December 2011 in our hospital. Control group were given oviduct intubation through fluid,observa-tion group in the oviduct intubation through fluidtechnology on treatment of traditional Chinese medicine. The situation of tubal patency in the control group and observation group and the effect evaluation on the patients with anxiety and depression were compared. Results In the control group after treatment, the blocking extent eased, from 86% to 40%, unobstructed degree increased from 5% to 45%, with significant difference before the treatment (P<0.05); Observation group after treatment, the blocking extent eased, from 86% to 40%, unobstructed degree increased from 6% to 88%, with significant difference than before treatment (P<0.05); unobstructed degrees after treatment, the observation group was obviously higher than that of control group, the difference was statistically significant(P<0.05).After treatment, two groups of patients with anxiety and depression were improved, the anxiety and depression of observation group was sig-nificantly lower than the control group,the difference was statistically significant(P<0.05). Conclusion Salpingitis us-ing dredging technology to have certain effect, but combined with traditional Chinese and western medicine treatment of fallopian tube obstruction curative effect is distinct.
8.The morbidity and mortality trend and prediction of lung cancer in residents of Nangang District of Harbin in China during the past 10 years.
Xiwen SUN ; Wei LIU ; Shuling WU ; Huili HAN ; Yingji LIN ; Xudong DAI
Chinese Journal of Lung Cancer 2005;8(6):514-517
BACKGROUNDIt is not clear yet about the secular changes of morbidity and mortality trend of lung cancer in residents of Nangang District of Harbin in China. The aim of this study is to estimate the trend of lung cancer morbidity and mortality in residents of Nangang District from 1992 to 2001 and to predict their levels in the future 5 years.
METHODSData were collected from the annual statistic reports on cancer death cause from Nangang District in Harbin. The classification of death cause was made according to the ICD-9. Predictions about morbidity and mortality were made by the gray system GM(1,1).
RESULTSDuring the past 10 years, the morbidity and mortality of lung cancer were placed in uptrend slowly. The average morbidity and mortality of lung cancer were 44.75 per 100000 and 41.37 per 100000 respectively, and lung cancer was the first leading cancer for both episode and death of malignant tumors. The proportions of lung cancer were 25.91% and 33.29% for episode and death in all malignant tumors respectively. A half patients with lung cancer was 20-64 years old. Predictive morbidity and mortality of lung cancer would be 47.79/100000 and 44.81/100000 for men and 45.80/100000 and 42.02/100000 for women respectively.
CONCLUSIONSThe morbidity and mortality of lung cancer show a slowly increasing trend. Lung cancer is one of main malignant tumors among people of 20-64 years old. The gradually aging population, environmental pollution and individual unhealthy living habits are the important factors of lung cancer increasing.
9.Negative interference by calcium dobesilate in uricase-peroxidase coupled assays of serum uric acid
Xiuzhi GUO ; Jiangtao ZHANG ; Li′an HOU ; Jie WU ; Songlin YU ; Huiling FANG ; Xinqi CHENG ; Liangyu XIA ; Lin ZHANG ; Zhihong Qi ; Shuling CHI ; Dawei TONG ; Yingyin HAO ; Ling QIU
Chinese Journal of Laboratory Medicine 2015;(9):600-604
Objective To assess the interference by calcium dobesilatein 7 peroxidase-baseduric acid assays and to determine its clinical significance.Methods In the in vitro experiments, uric acid in pooled serum with final concentrations of calcium dobesilate additions (0, 2, 4, 8, 16, 32, and 64μg/ml) were measured by 7 peroxidase-based assays.Percent Bias (%) was calculated relative to the drug-free specimen.In the in vivo experiments, changes in serum uric acid and calcium dobesilate concentrations were observed before and after calcium dobesilate administration ( baseline, 0 h, 1 h, 2 h, 3 h, 4 h, 6 h ) involunteers.The interference in different assays was assessed compared with LC-IDMS/MS method. Calcium dobesilate levels in 40 specimens from those taking calcium dobesilate were measured by HPLC method.Of the 40 specimens, 10 were selected to analyse the levels of uric acid by both peroxidase and UV measurement method to assess the impact in clinical status.Results In the in vitro study, concentrations of uric acid measured by 7 peroxidase-based assays were reduced by -6.3%to -21.2%compared with drug-free serum, when theconcentration of calcium dobesilate was16μg/ml.In the in vivo study, comparedto UA levels at 0 h, the biasesof serum uric acid determined by peroxidase method after calcium dobesilate administration(1 h, 2 h, 3 h, 4 h, 6 h) were of -3.33%, -6.79%, -7.49%, -6.07%, -4.09%, respectively.The observed uric acid concentrations for 8 participants measured by enzymatic assays were inhibited by -3.75% to -6.89% at 0 hour and by -16.9% to-22.22% at 2 hours relative to the concentrations measured by the LC-IDMS/MS method. Conclusions Calcium dobesilate produced a clinically significant negative interference with uric acid in all peroxidase-based uric acid assays,which may result in false evaluation of uric acid level in clinical status.Significant differences in the degree of interference were observed among the assays.
10.Application value of aortic dissection detection risk score in diagnosis of acute aortic syndromes: analysis of 342 patients with acute chest pain enclosed
Shuling LAI ; Jiyan LIN ; Jiaquan LIU ; Minwei ZHANG
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2017;24(5):473-476
Objective To investigate the value of aortic dissection detection (ADD) risk score in the diagnosis of acute aortic syndromes (AAS). Methods Three hundred and forty-two patients with acute chest pain or back pain admitted to the Department of Emergency of the First Affiliated Hospital of Xiamen University from January 2013 to April 2016 were enrolled. At last, 71 patients were definitely diagnosed as AAS (AAS group), and 271 cases were diagnosed as non-AAS (non-AAS group). Furthermore, according to the ADD risk score, they were subdivided into two groups: low-risk (ADD score ≤ 1) and high risk (ADD score >1) subgroups. In the two groups, the ADD risk indexes and the proportions of patients with different risk scores were observed; the receiver operating characteristic curve (ROC curve) was drawn to evaluate the value of ADD risk score for diagnosing AAS. Results Compared with the non-AAS group, the proportions of patients in AAS group with indicators of high-risk pain characteristics, such as sudden pain and laceration-like pain were increased significantly [83.1% (59/71) vs. 31.0% (84/271), 29.6% (21/71) vs. 0 (0/271)];meanwhile, the proportions of patients with high-risk physical examination indicators, such as systolic blood pressure differences among the 4 extremities and the defect of local nerve function in AAS group were also significantly increased [23.9% (17/71) vs. 0 (0/271), 11.3% (8/71) vs. 0 (0/271), both P < 0.05]; the proportion of patients with high risk AAS score in AAS group was higher than that in the non-AAS group [66.2% (47/71) vs. 1.5% (4/271), P < 0.01]. The sensitivity of ADD score ≥ 1 for diagnosis of AAS and area under ROC curve (AUC) were all higher than those of ADD score ≥2 (sensitivity: 98.6% vs. 66.2%, AUC: 0.819 vs. 0.564), moreover, the specificity and the positive predictive value of ADD score ≥ 2 for diagnosis of AAS were higher than those of ADD score ≥ 1 (98.5% vs. 59.8%, 92.2% vs. 39.1%respectively). When the ADD risk score ≥ 1, its odds ratio (OR) = 104.0, 95% confidence interval (CI) was 0.761-0.877, P = 0.000; while ADD risk score ≥ 2, OR = 130.7, 95%CI was 0.516-0.612, P = 0.003. Conclusion It is shown that when ADD risk score (> 1) is used to diagnose AAS, it has relatively high sensitivity, when ADD score being high risk (> 1 score) is applied to diagnose AAS, its specificity is high, thus ADD risk score has important value in helping the early diagnosis of AAS.