1.The experimental study of the cytotoxicity to breast cancer cell line using modified T lymphocyte by TCRVpT.1 gene
Licai HU ; Shudong QIU ; Shulin HUANG
Chinese Journal of Immunology 1985;0(02):-
Objective: To investigate the influence of transfection of TCRVJ37.1 gene in cytotoxicity of PBMC of healthy donor to breast cancer cell line MCF-7/S. Methods :pcDN*31vp'1 packaged by lipofectamine were transfected to healthy donor's PBMC,Flow Cytometer Analysis and MTT Colorimetric Assay were used respectively to test the expression of pcDNA3.1VB7.1 gene and the cytotoxicity of PBMC before and after gene transfection of healthy donor to breast cancer cell line MCF-7/S. Results: The expression of TCRVJ37.1 gene after transfection was obviously higher.There was distinguish differences of cytotoxicity before and after gene transfection. Conclusion:The modified healthy donor's PBMC by TCR gene could have stronger cytotoxicity to breast cancer cell line.
2.Effects of medical polyacrylamide hydrogel on human fibroblasts: a transmission electron microscopic study
Xiao WANG ; Shulin QIU ; Shuxia WANG
Chinese Journal of Medical Aesthetics and Cosmetology 2010;16(4):256-258
Objecttive To explore the effect of medical polyacrylamide hydrogel on the ultrastructure of human fibroblasts.Methods 22 hyperplastic fibropeplos around the medical polyacrylamide hydrogel in mammaries after augmentation mammoplasty by injection of medical polyacrylamide hydrogel were obtained by operations.The tissues were observed with the transmission electron microscope and at the same time photographs taken.Results Most of cell organoles dissolved and disappeared;Most of the cristaes and membranes of mitochondria coalesced or disappeared;rough endoplasmic reticulum expanded.Most of the cristaes and membranes coalesced or/and disappeared.The phenomenon of shed particle was observed in some of the rough endoplasmic reticulum.Conclusion The medical polyacrylamide hydrogel obviously injures the ultrastructures of human fibroblasts.
3.Changes in glomerular filtration barrier in rat models of acute exhaustive exercise after intervention with sodium houttuyfonate
Xixiu LIN ; Jiwang QIU ; Ziqiang LUO ; Shulin QU ; Yongqiang ZHAO
Chinese Journal of Tissue Engineering Research 2014;(36):5793-5798
BACKGROUND:In high-intensity exhaustive exercise process, the body must bear the exercise intensity decreasing splanchnic blood flow“ischemia”, at the same time, along with the movement of energy and material consumption, metabolite accumulation and oxidative stress in the body cause pathological damage, leading to a decline in exercise capacity. Thus, what is the impact on kidney filtration barrier? How to adapt to the change of renal tissue? Houttuynia cordata has the functions of heat clearing and detoxifying, dieresis for treating strangurtia, hemostatic, expel ing phlegm to arrest coughing and analgesia, if it has a protective effect on the renal injury caused by acute exhaustive exercise and its mechanism has not been reported in the literature. OBJECTIVE:To explore the effect of acute exhaustive exercise on kidney filtration barrier in rats and the intervention effect of Houttuynia cordata.METHODS:After resting and watching for 3 days, Sprague-Dawley rats received adaptive running for 15 minutes at a speed of 10 m/min on a 0° treadmil . A total of 24 rats, which can finish the running, were selected. They were divided into normal control group, exhaustive exercise group and dosed exhaustive exercise group according to the weight of layer (n=8). Rats in the exhaustive exercise group and dosed exhaustive exercise group on the 10° treadmil received once exhaustive exercise. Dosed exhaustive exercise group received intraperitoneal injection of sodium houttuyfonate 10 mL/kg at 30 minutes before exercises. The normal control group did not do any exercise. RESULTS AND CONCLUSION:Compared with the normal control group, serum urea nitrogen, serum creatinine, urine protein content, malondialdehyde concentration, renal cellapoptosis and apoptosis index were significantly increased, but nitric oxide content and nitric oxide synthase activity in the renal tissue were significantly deceased in the exhaustive exercise group (P<0.05 or 0.01). Glomerular filtration epithelial cells, the kidney filtration barrier of basement membrane and podocyte damage were obvious, showing abundant cellapoptosis, occasional y necrosis. Compared with the exhaustive exercise group, urine protein content, serum creatinine, malondialdehyde concentration, renal cellapoptosis and apoptosis index were significantly reduced, but nitric oxide content and nitric oxide synthase activity were significantly increased in the dosed exhaustive exercise group (P<0.05). No obvious pathological changes were detected, but apoptosis was visible. These findings confirmed that houttuynine made a reduction in renal cellinjury induced by exhaustive exercise and possibly significantly reduced apoptosis, increased nitric oxide synthase content, decreased malonaldehyde, and apparently increased superoxide dismutase activity, and final y protected injured renal tissue induced by exhaustive exercise.
4.Effect of botulinum toxin type A on children with odorihidrosis
Zeliang HE ; Julei ZHANG ; Jin LI ; Lingling LIU ; Chengliang ZHANG ; Yuanyuan YAO ; Zhenyang SUI ; Zeyi WU ; Shulin QIU ; Xiaodong LI
Chinese Journal of Medical Aesthetics and Cosmetology 2023;29(2):130-133
Objective:To investigate the effect of botulinum toxin type A on children with odorihidrosis.Methods:From March 2017 to February 2021, 121 children with odorihidrosis, including 48 males and 73 females, aged 13 to 17 (15.9±1.2) years, were admitted to the Burn and Plastic Surgery Department of the 980 Hospital of PLA. There were 24 cases in mild group, 50 cases in moderate group and 47 cases in severe group. Botulinum toxin A was injected into 20-50 points on each side, and 1 U was injected into each point. The total amount of botulinum toxin A was 50-100 U on both sides.Results:Three groups of children were evaluated for efficacy, 24 cases of mild group was significantly effective in 23 cases, accounting for 95.8%. In the moderate group, 46 (92.0%) of 50 cases showed obvious effect. 49 cases (98.0%) were effective; In the severe group, 40 cases (85.1%) showed obvious effect and 45 cases (95.7%) were effective. Three groups of children with different efficacy had no statistical significance ( P>0.05). The significant efficiency in mild and moderate groups was higher than that in severe group, and the difference was statistically significant ( P<0.05). Conclusions:Botulinum toxin type A is effective in the treatment of children with mild and moderate bromhidrosis, and is worthy of clinical application.
5.Thermal proteome profiling: a technique for a comprehensive assessment of protein status.
Yanhua QIU ; Bintao ZHAI ; Yubin BAI ; Shulin CHEN ; Jiyu ZHANG
Chinese Journal of Biotechnology 2022;38(10):3628-3637
Thermal proteome profiling (TPP) is a combination of cellular thermal shift assay (CETSA) and quantitative mass spectrometry (MS), also termed as MS-CETSA. TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures. Proteins can change their thermostability when interacting with small molecules (e.g., drugs or metabolites), nucleic acids, or other proteins or posttranslational modification, while TPP can identify target proteins based on the difference in thermostability with or without ligand-binding. At present, TPP has been applied to identify the targets and off-targets of drugs and interrogate protein-metabolite and protein-protein interactions. Due to limited understanding of this technology, this review introduced the principles, methods, applications, advantages and limitations of TPP.
Proteome
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Mass Spectrometry