Objective To explore the clinical significance of lantus plus acarbose applied for the treatment of elderly diabetes patieats.Methods 84 old diabetes patients treated in our hospital were randomly divided into experimental group and control group. The patients of experimental group were treated with lantus plus acarbose, the control group used normal insulin. To observe the therapeutic effect of two groups. Results The levels of fasting plasma glucose,2 hours postprandial glucose,glycosylated hemoglobin A1C and the occurrence of adverse reactions after the treatment were statistically significant ( P ＜ 0. 05 ). Conclusion Lantus plus acarbose was an effective treatment for elderly diabetes patients, and could obtain the ideal blood sugar and less complications, and clinical effect of the therapy was ideal, and it was worth used widely.

Object To analyze the rDNA ITS sequences between wi ld plants and cultivars of Trapa L. and study the utility in p hylogenesis and identification of these two groups. Methods The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions w ere analyzed by means of the software of Clustal and Mega 2.0. Result s The rDNA sequences of 234-236 bp ITS1, 220-221 bp ITS2 gene fragment , and 5.8 S rDNA for 164 bp evenly were obtained from ten populations of Trapa L. The intraspecific substitution varies from 0.22% to 2. 94%. The variable sites are 16 while informative sites are six. The phylogenet ic tree based on ITS data was set up by NJ method. Conclusion ITS sequence is a pretty good molecular marker which can identify wild plants of Trapa L. from their cultivars. Diversity of ITS in differen t populations is less at intraspecific level. It is infered that the plants of Trapa L. may be derived from the same population of one species .

Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Schistosoma japonicum cercariae were stained by 0.4%trypan blue 0.5%methylene blue?eosin?borax M.E.B 0.5%eosin 0.5%methy?lene blue and 0.05% neutral red respectively for 5 min then they were observed under a stereoscopic microscope. Results The dead cercariae were stained in the trypan blue M.E.B eosin and neutral red but unstained in the methylene blue. The vi?tal cercariae were unstained in all the five kinds of dyes. Conclusion The staining methods by using 0.4% trypan blue 0.5%M.E.B 0.5%eosin and 0.05%neutral red can be used to determine the viability of S. japonicum cercariae.