Background and purpose:Cervical cancer is one of the common disease in women in china.Radical surgery remains the first choice for patients with early-stage cervical cancer.However,the majority of patients with bulky disease have lymph nodes or metastases when diagnosed.Palatinum-based neoadjuvant chemotherapy has been utilized in locally advanced cervical cancer with the aim of improving response and survival.The study was to evaluate the effect of neoadjuvant chemotherapy followed by radical surgery for the patients with locally bulky Ⅰ_(B2) and Ⅱ_A cervical carcinoma.Methods:Between September 2003 and February 2004,51 untreated patients with Ⅰ_(B2) and Ⅱ_A cervical carcinoma were enrolled in study group,and were treated with neoadjuvant chemotherapy with cisplatin,vincristine and bleomylin for one cycle followed by radical hysterectomy and pelvic lymphadenectomy;another 51 patients as control group were treated with radical surgery alone.The tumor size,depth of stromal invasion,lymph node metastasis and involvement of surgical specimens were assessed.Results:The overall clinical response rate was 41.2%.There were no complete and progressive ones.The blood loss during operation was 116.82?45.50ml,it was significantly lower than that in control group(P

The hypothesis of tumor stem cells believes that a small proportion of cells which have the stemness property reside in tumor. This population is the cause of tumor formation, growth, metastasis and relapse. Only the therapy targeting this population could make tumors curable. At present, most anti-cancer protocols only kill the majority of differentiated tumor cells and hardly affect the tumor stem cells. That is why most of the therapies do not achieve good results. Disrupting the pathways and niche which regulate the tumor stem cells’ self-renewal, or inducing the tumor stem cells’ differentiation, or targeting the surface markers which distinguish the tumor stem cells from normal stem cells are all probable strategies.

Anesthesia modalities for endovascular treatment of acute ischemic stroke include general anesthesia and conscious sedation.Several recent studies have shown that the general anesthesia is associated with the poor outcome of endovascular treatment in patients with acute ischemic stroke.The underlying reason of causing worse outcome is unknown.It may be associated with hemodynamic instability and hypotension.Currently,it is considered that both general anesthesia and conscious sedation have their advantages and disadvantages.We must choose individualize anesthesia modalities for each patient before we obtain highquality evidence.

Objective To explore the pathogenicity of Campylobacter jejuni(CJ)O∶19 strain after deletion of neuB1.Methods The mutant of CJ O∶19 by deleting neuB1,which LPS is deficient in sialic acid,was compared with the homologous wild strain by assaying their sensitivity to bactericidal activity,motility and autoagglutination of 10% normal human serum(NHS).Results The sensitivity of the mutant strain to bactericidal activity of serum was superior to that of the wild strain.The survival rate of the mutant strain was(20.6?7.4)% and(9.6?3.6)% after incubation with 10% NHS for 15 min and 60 min,that of wild strain was(36.9?5.9)% and(15.5?4.3)% respectively.The wild and mutant strains showed no significant difference in sensitivity to motility and autoagglutination of NHS.Conclusion The mutant strain possesses remarkable pathogenicity so that an inactivated vaccine from the mutant strain should be first considered.

e factors of elder age, abdominal surgeries and malignant tumor.

OBJECTIVE:To observe the clinical efficacy of compound glycyrrhizin combining desloratadine in the treat?ment of chronic urticaria.METHODS:103patients with chronic urticaria were randomly divided into treatment group which were treated with compound glycyrrhizin plus desloratadine and controlled group which were treated with single desloratadine.The courses of treatment for both groups were2weeks.RESULTS:The total effective rates of the treatment group and the controlled group were96.15%and82.35%,respectively.There were significant differences between the2groups(P

Objective To verify the applicable significance of confocal scanning laser retinal tomography in age-related macular degeneration (AMD). Methods Heidelberg confocal scanning laser tomography was used to measure 75 eyes of 59 patients with AMD, including 25 eyes of 20 patients with exudative AMD, 25 eyes of 16 patients with atrophic AMD , and 25 eyes of 23 patients with macular drusen. The differences of the width, volume and maximum height of Z profile signal of macula were analyzed. Results Z profile signal width in macular tomography of exudative AMD was wider than that of macular drusen; maximum height and volume in macular tomography of exudative AMD were larger than that of macular drusen (P

Objective To survey the CT radiation dose to cancer patients,and to compare it with diagnostic reference level (DRL) provided by newly issued national standard.Methods Computed tomography dose index weighted (CTDIw) of 5 CT equipments was measured by the standard phantom with typical scanning protocol and radiation dose parameters including average computed tomography dose index volume (CTDIvol),dose length product (DLP),75％ DLP with 900 adult and 300 pediatric cancer patients were collected.According to age stage,effective dose was estimated.Results The measured CTDIw for head scanning was on the same level with DRL,while lumbar vertebra and abdominal scanning were lower than DRL.To adults,DLP of cerebrovascular enhanced scanning and effective dose of abdominal enhanced scanning were the highest.To pediatric patients,there was no significant difference in DLP among different ages.Conclusion CTDI only reflects the dose contribution from a slice,but do not reflect the cumulative dose.For the patients need multiphase scanning,because they may be exposed to high dose,national standard should provide DLP value as the diagnostic reference level.Using individual scanning protocol is an effective method to reduce radiation dose of pediatric patient.

AIM To investigate the hypoglycemic effect of buccally delivered insulin solution (INS SOL) in normal rats. METHODS The hypoglycemic response was examined after buccal delivery of INS SOL co administered with various absorption enhancers. The pharmacological bioavailability (PA) was used to evaluate the absorption enhancement of INS SOL from the buccal cavity under various conditions compared with subcutaneous injection. RESULTS In the absence of enhancers, the PA was low(6 8%) after buccal delivery of INS SOL. However, the concomitant administration of sodium lauryl phosphate, sodium deoxycholate, Brij78 as well as lecithin appeared to be more effective in increasing the hypoglycemic effects of insulin. INS SOL (5 ??kg -1 ) containing 5% Brij 78 had the highest PA of 33%. CONCLUSION The use of proper absorption enhancers is useful for improving the buccal absorption of insulin.

Objective To evaluate the efficacy and safety of different doses of prednisone combined with cyclosporine A(CSA) on the treatment of idiopathic membranous nephropathy (IMN). Methods The data of 64 patients with nephrotic syndrome (NS) diagnosed as IMN by renal biopsy were retrospectively analyzed. Median follow?up time was 10 (7, 19) months. The subjects were divided into 2 groups according to different prednisone dosage. Thirty?two cases were in the low?dose group:prednisone 0.15 mg·kg-1·d-1+CSA, and 32 cases in the moderate?dose group:prednisone 0.4?0.5 mg· kg-1·d-1+CSA. Clinical and laboratory data were collected at baseline, 1, 3, and 6 months after treatment. During follow?up, cumulative recurrence rate and adverse reactions after treatment were recorded. Results Serum albumin (sALB) were significantly increased and 24 h urinary protein (24hUP) significantly decreased after treatment for 1, 3, 6 months compared with baseline data in the two groups. Serum creatine (Scr) increased after treatment with time. The elevation of sALB and the reduction of 24hUP in the moderate?dose group were higher than that of low?dose group at 6 months after treatment (P<0.05). The effective rate of the low?dose and moderate?dose group was 65.6% and 87.5% at 6 months after treatment, respectively (χ2=4.267, P=0.039). Comparison of different doses of CSA in two groups at 6 months after treatment, in low?dose group: the effective rates of CSA<3 mg· kg-1·d-1 and >3 mg·kg-1·d-1 subgroup were 76.5% and 53.3%, respectively (P=0.296); In moderate?dose group:the effective rates of CSA<3 mg·kg-1·d-1 and>3 mg·kg-1·d-1 subgroup were 89.5%and 84.6%, respectively (P=0.077); there were similar effects in patients treated with different dose CSA in the two groups. About 20.4% of the total patients relapsed when followed up for 18 months (low dose group vs moderate?dose group: 9.5% vs 28.6%, P=0.136), which most occurred after prednisone withdrawal or during the reduction of cyclosporine. Renal function decreased in 57.8% patients (low dose group vs moderate?dose group:50%vs 65.6%), mainly in the elderly (9/11) and the long course of treatment of CSA. There was no significant difference on adverse reactions between the two groups (P>0.05). Renal function in patients with high Scr or high blood trough concentration of cyclosporine was difficult to fully recover. Conclusions Remission rate is lower in low?dose prednisone combined with cyclosporine than the moderate?dose group in the treatment of IMN for 6 months. The recurrence rate of IMN or the incidence of adverse reactions are similar between the two groups. Induction therapy of IMN with cyclosporin<3 mg·kg-1·d-1 is safe and effective. The incidence of renal function reduction in the elderly is high, and the renal function is difficult to restore in patients with Scr exceeding normal upper limits.