1.Effect of DFMO on the Growth of K562 Cells and Its Association with Telomerase Activity
Mci XU ; Muzhen FAN ; Shulan CAO
Chinese Journal of Cancer Biotherapy 2000;7(4):247-250
Objective: To investigate the effects of DFMO on the growth characteristics, apoptosis and activity of telomerase of K562 cells. Methods: The growth rate, phase distribution of cell cycle and apoptosis of treated cells with DFMO were detected by cell count, morphological assay, FCM analysis and DNA electrophoresis, respectively. The telomerase activity was examined by telomeric repeat amplification protocol(TRAP). Results: In K562 cells treated with DFMO, the growth rate was markedly retarded. The increase in G1 -phase cells and decrease in s-phase population and induction of apoptosis were observed. The activity of telomerase of treated cells was inhibited. Conclusion: The growth inhibition and apoptosis induction of K562 cells treated by DFMO were associated with suppressed telomerase activity. It is suggested that inactivation of telomerase in cancer cells conld be one of important events in antitumor molecular mechanism of inhibition of polyamine biosynthesis.
2.Relationship between phosphodiesterase 4D gene polymorphism and ischemic cerebral vascular disease
Shulan XU ; Yingdong ZHANG ; Xingjian LIN
Journal of Clinical Neurology 1995;0(04):-
0.05) . Conclusions The A-allele in rs918592 may one of the risk factors in development of ICVD in the Han people in China. PDE4D gene may be not included glycometabolic mechanism to effect ICVD.
3.Strategy for keeping efficient expression and pharmacodynamics in reducing rAAV gene medicine immune response based on the decrease of vector dosage.
Guohai ZHANG ; Shulan ZENG ; Ruian XU
Acta Pharmaceutica Sinica 2013;48(3):305-14
How to reduce immune response is an unprecedented challenge for rAAV gene medicine. Recent studies suggested that lowering dosage of the vector used could reduce immune response caused by rAAV gene medicine. Nevertheless, it would also decrease the transgene expression, leading to failure of gene treatment. It is therefore important to take appropriate steps to maintain high gene expression level and pharmacodynamic, while the dosage of rAAV used is reduced. Here, steps to enhancing gene therapy, such as optimization of the administration, reconstruction of the viral vector and selection of the promoter, are discussed in order to achieve maximum outcome.
4.A clinical retrospective study on Osstem MS one-stage implant restoration of small edentulous space in the mandibular anterior region
Yan GAO ; Shulan XU ; Lei ZHOU ; Shuo YANG ; Zhen ZHOU
Journal of Practical Stomatology 2015;(5):639-643
Objective:To assess the clinical efficacy of two different diameter Osstem MS one-stage implant restoration of small edentu-lous space in the mandibular anterior region.Methods:85 patients were treated by Osstem MS one-stage implant with the diameter of 2.5 mm(n =66)and 3.0 mm(n =66)respectively for the restoration of small edentulous space in mandibular anterior region.The mesi-al and distal marginal bone level and soft tissue were statistically analyzed after 1 2 and 24 months of functional load.The implant survival rate was evaluated according to Wheeler's survival criteria.Results:The survival rate of the implants was 1 00%.The mean changes in marginal bone level(mm)on the mesial side of 2.5 mm and 3.0 mm diameter implants were 0.275 ±0.638 and 0.098 ±0.31 9,distal aspects were 0.360 ±0.588 and 0.1 09 ±0.323 after 1 2 months of functional load;while 0.299 ±0.672 and 0.099 ±0.31 8,0.381 ± 0.581 and 0.1 07 ±0.31 9 after 24 months of functional load.The mesial and distal marginal bone loss of 2.5 mm diameter implant was greater than that of 3.0 mm after 1 2 and 24 months of functional load(P <0.05).No significant change on the marginal bone level was found aomog the same diameter implants from 1 2 to 24 month observation(P >0.05).No relevant complication of peri-implant soft tissue was shown.Conclusion:Favorable clinical effects including function and aesthetics can be achieved by Osstem MS one-stage implant with the diameter of 2.5 mm or 3.0 mm for the restoration of small edentulous space in the mandibular anterior region,however,the mar-ginal bone loss was greater around 2.5 mm diameter implant.
5.Clinical study of the influence on marginal bone levers when open healing of implants with platform ;switching
Shulan XU ; Shuo YANG ; Xijuan XIAO ; Xianglong DING ; Fang JIA
The Journal of Practical Medicine 2016;32(12):2020-2022
Objective The study examined the influence on marginal bone levers at implants which implants were assigned to open healing and implant-abutment connection restored according to a platform-switching concept. Methods One implant were placed in the first molar of mandible of 48 patients , randomly assigned to open (OH) or submerge (SH) healing. Standardized radiographs were obtained after implant surgery, when implant transfer and crown mounting, and 6 ,12 and 24 months after implants restoration, then evaluated for implant-bone-lever alterations(ΔIBL). Results After 6 months after implants restoration,ΔIBL were (-0.25 ± 0.08)mm mm(OH)and (-0.26 ± 0.12)mm(SH). After 12 months follow-up, ΔIBL were (-0.29 ± 0.13)mm (OH)and (-0.31 ± 0.14) mm(SH), and after 24 months later, ΔIBLwere (-0.34 ± 0.15)mm(OH)and (-0.33 ± 0.11)mm (SH). Conclusions Platform-switched implants showed very limited peri-implant bone-lever alterations. The healing-mode neither affected the total amount nor the temporal patterns of ΔIBL.
6.Effect of Sangge Mixture on Cerebral Circulation and Metabolism in Rabbits
Fuwen WANG ; Jie LI ; Yan ZHU ; Shulan XU
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
The effect of Sangge Mixture (SM) on cerebral circulation and metabolism in rabbits was observed.The results showed that SM increased the blood flow of brain and cerebral cortex in a dose-dependent manner and had no effect on the blood flow of femoral arteries. Meanwhile, SM markedly increase the oxygen consumption and glucose intake in rabbit brain. It is suggested that SM can improve cerebral circulation, with a certain degree of selectivity.
7.ESBLs′ Genotypes of Escherichia coli and Klebsiella spp Isolated from Xinjiang: A Study
Min DU ; Xu CHEN ; Shangzhu MA ; Jiyun MA ; Shulan ZHANG ; Qiwen YANG ; Yingchun XU
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To investigate the resistant patterns of ESBLs-producing Escherichia coli and Klebsiella spp isolated from 3 hospitals in Xinjiang and to determine the genotypes of ESBLs. METHODS Using 2-fold agar dilution test to determine MICs of ten antibiotics.Using polymerase chain reaction and DNA sequencing to determine ESBLs genotype;using isoelectric focusing to determine the isoelectric points of ?-lactamases produced by strains in which no ESBLs genes were amplified by PCR. RESULTS Strains had low susceptibility to aztreonam,cefotaxime and ceftazidime.The susceptibility to cefepime,cefoperazone/sulbactam,cefmetazole,piperacillin/tazobactam,and meropenem were relatively high.Fifty five strains mainly carried blaCTX-M-1 subgroup gene and blaCTX-M-9subgroup gene,and blaSHV-12 gene were also detected. CONCLUSIONS blaCTX-M-1 Group gene and blaCTX-M-9 group gene are prevalent in three hospitals of Xinjiang,blaSHV-12 gene also plays a part role in leading to resistance of E.coli and Klebsiella spp to ?-lactams.
8.Correlation between nucleated red blood cell count and perinatal asphyxia in neonates
Ning TAN ; Huabao PENG ; Shulan FEI ; Yonghong WANG ; Wenyuan SHI ; Guizhen XU ; Zhouli WU
Chinese Journal of General Practitioners 2014;13(4):293-295
To explore the correlation between nucleated red blood cell (NRBC) count and perinatal asphyxia in neonates.Full-term newborns born from May 2011 to November 2012 were recruited and divided into perinatal asphyxia (n =40) and normal (n =30) groups.Apgar score was recorded immediately at delivery.The umbilical arterial blood was also collected into anticoagulant-treated tube and NRBC was counted by Japan OlympusCX41 biological microscope.NRBC count for perinatal asphyxia group [(10.70 ± 2.61)/100 WBC] was significant higher than that for normal group [(2.67 ± 0.35)/100 WBC].A statistically significant negative correlation existed between NRBC and umbilical arterial blood pH,Apgar score at 1 min,BE value (r =-0.802,P < 0.05 ; r =-0.639,P < 0.05 ; r =-0.566,P < 0.05).Associated with perinatal asphyxia in neonates,NRBC may be used as a simple index for assessing the severity of neonatal perinatal asphyxia.
9.Effect of three wavelength lasers on mRNA expression of types Ⅰ and Ⅲ procollagen in fibroblasts
Yanjing XU ; Xiaojing YU ; Jingpeng WANG ; Shulan GUO ; Dalie LIU ; Qin LI
Chinese Journal of Medical Aesthetics and Cosmetology 2016;22(4):236-239
Objective To investigate the effects of different wavelength (595 nm,755 nm and 1064 nm) lasers on the mRNA expression of types Ⅰ and Ⅲ procollagen in cultured fibroblasts.Methods Fibroblasts from Kunming mice were cultured in vitro.They were divided into 595 nm laser group,755 nm laser group,1064 nm laser group and no laser irradiating group.The mRNA expression of the types Ⅰ and Ⅲ procollagen was detected by RT-PCR.Results The mRNA expression level of type Ⅰ procollagen in 1064 nm group was higher than that of 755 nm group,595 nm group and control group (P<0.05).The expression levels of 755 nm group and 595 nm group were higher than that of control group (P<0.05).But the difference between 755 nm group and 595 nm group was not statistically significant (P>0.05).The mRNA expression level of type Ⅲ procollagen in 1064 nm group was higher than that in 755 nm group,595 nm group and control group (P<0.05).755 nm group had higher expression than 595 nm group and control group (P<0.05).But the difference between 595 nm group and the control group was not statistically significant (P > 0.05).Conclusions Three wavelength lasers can directly promote mRNA expression of types Ⅰ and Ⅲ procollagen in fibroblasts.595 nm laser mainly promotes mRNA expression of type Ⅰ procollagen,and 755 nm laser promotes more mRNA expression of type Ⅲ procollagen than 595 nm laser.The most mRNA expression of types Ⅰ and Ⅲ procollagen is promoted by 1064 nm laser.
10.Removal of fractured implants and immediate reimplantation:Report of a case
Shulan XU ; Zhao WANG ; Zhongxiong YAO ; Jinquan MU ; Zhigang ZHAO ; Shuo YANG
Journal of Practical Stomatology 2014;(4):583-585
Fracture of multiple implants is rare.This report describes 1 case with the fracture of multiple implants related to occlusal over-load,bruxism,periodontitis and other factors in the posterior position.The fractured implants were removed and immediate reimplantation was performed.The panoramic film showed that the implants were integrated successfully 12 months after prosthesis.