Hypercholesterolemia is an important risk factor of cardiovascular disease,which is related to complications and mortality of cardiovascular disease.A lot of researches show that some genes polymorphism was related to hypercholesterolemia.This paper introduces the hypercholesterolemia,including LDLR(low-density lipoprotein),apo(apolipoprotein) and PCSK9(proprotein convertase subtilisin kexin type 9),etc,and summed up the relationship between the genes polymorphism and hypercholesterolemia.

CD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily and is involved in various physiological and pathological activities.Highly expressed on the surface of many tumor cells,CD147 can promote tumor metastasis,angiogenesis and anchorage-independent growth.Targeting CD147 in cancer appears a promising therapeutic strategy.This review outlines the recent findings concerning the role of CD147 in tumor progression.

Vascular endothelial growth factor(VEGF) is high expressed in the organization of renal cell carcinoma and is mainly regulated by VHL gene and hypoxic of organization.Moreover,it can be used as a biomarker to judge renal cell carcinoma tumor progression and prognostic.In recent years,anti-VEGF targeted drugs are used for the treatment of renal cell carcinoma and receive meaningful clinical benefits.

Objective To study the action of the lymphangial immunochemotherapy on the advanced stomach cancer. Methods Group of therapy: To inject the chemotherapeutic medicine and biological response modifiers (BRM) absorbed by active carbon through the stomach mucosa by means of gastroscope and through the stomach serosa by means of surgical operation. Group of contrast: To inject the aqueous solution of the chemotherapeutic medicine and BRM through the arteria femoralis by means of the intervention. Then we examined the immune activity of the blood cell.Results There is a marked difference between the lymphangial immunochemotherapy and the blood immunochemotherapy in the immune activity of the blood cell. Conclusion The lymphangial immunochemotherapy can promote the apoptosis of the transferred cancer in the lymph system and enhance the immune activity of the blood cell.

Objective:To study relativity between tumor metastasis and the level of immune competent cells from patients with malignant carcinoma.Methods:The percentage of NK cells and CTL cells and monocyte/macrophage cells were measured and analyzed in tumor tissue and peripheral blood from patients with malignant carcinoma by flow cytometry.Results:NK cells, CTL cells and monocyte/macrophage cells of tumor tissue and peripheral blood were lower than those of normal control groups ( P

BACKGROUND:Percutaneous vertebroplasty technique has become an effective means for clinical treatment of spinal osteoporotic fractures, but there is the risk of bone cement leakage.
OBJECTIVE:To explore the clinical effect of percutaneous vertebroplasty with low-dose bone cement for acute osteoporotic vertebral compression fractures.
METHODS:From September 2008 to February 2011, 32 patients with osteoporotic compression fractures were treated by percutaneous vertebroplasty. According to the dose of bone cement, the patients were divided into low dose group (2-4 mL) and routine dose group (4-6 mL). In addition, another patients who were hospitalized over the same period for acute osteoporotic compression and could not receive vertebroplasty due to urgical contraindication served as control group.
RESULTS AND CONCLUSION:The pain relief and vertebral height restoration rate of the low dose group and routine dose group were significantly better than the control group (P<0.05). The bone cement leakage rate and last fol ow-up incidence of adjacent segment vertebral fractures of the low dose group were also significantly lower than those in the routine dose group (P<0.05). Vertebroplasty with modified low dose bone cement can achieve satisfactory clinical effects, and effectively reduce the leakage of bone cement and incidence of adjacent segment secondary fractures.

AIM　The purpose is to study the influences on serum insulin and glucose by different fasting time in alloxan (ALX)-induced diabetic mice. METHODS　Normal mice of serum glucose were determined at different fasting time. According to different fasting time (0 h, 6 h, 12 h, 18 h and 24 h), mice were divided into 5 diabetic experimental model (DEM) groups induced by ip ALX 200 mg/kg and 1 control group. Serum glucose (SG) and body weight were determined at experimental d0, d3, d8 and d13 and serum insulin concentration (SIC) at experimental d13. The death rate and succeeding rate were also observed during experimental time. RESULTS　Along with prolonged fasting time, SG、death rate and succeeding rate were increased in DEM mice, SG of normal mice, and SIC and body weight of DEM mice were decreased. Results showed that the optimal fasting time of ALX-induced diabetic mice was 12～18 h a lower death rate and impairment to pancreatic islets. Fasting 18h diabetic mice had higher succeeding since during this time rate、lower death rate and longer hyperglycemia time. CONCLUSION　This study indicated SG of mice in vivo had a protective effect against impairment action of pancreatic islets by ALX.

AIM: To study the changes of endothelin system during chronic heart failure (CHF) and imply the relationship between endothelin system and the course of CHF by observing the mRNA expression of endothelin receptors (ET_AR and ET_BR) and PreproET_1 in early stage and later stage of CHF caused by left coronary artery ligation. METHODS: The mRNA expression of ET_A, ET_B receptors and PreproET_1 were detected by RT-PCR technique. The plasma concentrations of ET_1 and ANP were determined by RIA method. RESULTS: The plasma concentrations of ET_1 and ANP, and the mRNA expression of ET receptors and PreproET_1 in the lefe ventricle increased significantly in early stage (myocardial infarction 10 d). While the plasma concentrations of ET_1 and ANP in later stage (myocardial infarction 70 d) were higher than those in the early stage. However, the mRNA expression of ET_AR, ET_BR and PreproET_1 decreased significantly. The mRNA expression of ET_AR in myocardial infarction (MI) 70 d rats had no difference with those in sham-operated rats, and the mRNA expression of ET_BR and PreproET_1 in MI 70 d rats was lower significantly than those in MI 10 d rats, but significantly higher than those in sham-operated rats. CONCLUSION: The changes of ET receptors and PreproET_1 mRNA expression are involved in the cardiac function modulation during the different stages in chronic heart failure. [

With the increasing of doctor-patient conflicts,the communication between them gradually becomes a critical element in medical service activities.How to improve the doctor-patient communication is an important content in resident communication skill training.Oncology is a developing discipline with fast development and high risk and residents in oncology department need more communications with patients in the era which individualized treatment is emphasized.Systematization and institutionalization of the training system of doctor-patient communication is beneficial to popularizing doctor-patient communication experiences,protecting the rights and interests of them and ensuring the smooth process of medical treatment.

Objective: To clone and express the preferentially expressed antigen of melanoma (PRAME) gene in E.coli. Methods: The cDNA encoding human PRAME gene was extracted from human AML cell line HL-60 and amplified by RT-PCR, then the PRAME gene was inserted into plasmid PGEM-T-easy. After sequenced, it was cloned into the prokaryotic expression vector pGEX-4T-2 to construct a clone with high level expression and the recombinant plasmid was cloned in E.coli. Results:The protein product reached the highest level at 5 h after IPTG induction. Conclusion: Since PRAME is only expressed at low levels in a few normal tissues and encodes an antigen recognized by autologous cytotoxic T lymphocytes, while expressed at high levels in patients with leukemia, it might be a new targeting candidate for tumor immunotherapy.