The platelet gel (PG) is mainly made of platelet rich plasma (PRP), which has been increasingly and widely used as a source of growth factors .PG releases platelet derived growth factor , vascular endothelial cell growth factor , transforming growth factor β, epidermal growth factor , and insulin like growth factor 1.These growth factors are necessary for wound healing .Re-cently studies demonstrated that PRP and PG were effective in treating ulcers , burning, osteoarthritis and arthromeningitis .This article will review the bionomics , the mechanism of promoting wound healing and clinical applications of PG .

Drug-induced pancreatitis (DIP)belongs to gastrointestinal drug-induced diseases.With the wide use of drugs,the number of drugs that may induce DIP is increasing.Since there are not specific clinical indicators for detecting DIP or a complete diagnostic and thera-peutic process for DIP,its diagnosis is often missed.The epidemiology and pathogenesis of DIP,as well as drugs inducing DIP and their classifications,are reviewed.It is stressed that careful collection of medication history of DIP patients and timely discontinuation of suspected pharmacological agents are the key to diagnosis and treatment of this disease,and the patients should be informed of the related drugs that in-duce DIP to prevent its recurrence.

Pluripotent stem cells are type of cells that can differentiate into cells of ectoderm,mesoderm and endoderm layers.Cells isolated from developing embryos such as embryonic stem cells,embryonic germ cells,as well as cells derived from adult tissues and bone marrow and testis,have been proved to be pluripotent.Recent studies demonstrated that differentiated cells from adult could be reprogrammed to become pluripotent by genetic manipulation.The characteristics of pluripotent cells from different origins are summarized in this review,and the possible relationship between those populations is discussed.

Objective To fully understand the medicinal plants of Fritillaria L.,the physicochemical properties of starch in two species of Fritillaria L.,F.thunbergii and F.ussurensis.were investigated by means of various analytical methods.Methods The properties of starch in the two different species of Fritillaria L.were compared by X-ray diffraction,scanning electron microscope(SEM) and themogravimetric analysis(TGA).Results The crystal type of starch in the two species of Fritillaria L.was the characteristic B-type which was in consistent with that of potato starch.The degrees of crystallinity of F.thunbergii starch and F.ussurensis starch were about 29.9% and 20.1%,respectively.However,the degree of crystallinity of the potato starch was 44.9%.From the crystallinity degree of the starch in two species of Fritillaria L.,it could be concluded that the content of amylose in F.ussurensis starch was higher than that in F.thunbergii starch.The granule size of the starch in two species of Fritillaria L.ranged from 5 to 40 ?m,which were all smaller than that of the potato.The starch granule in two species of Fritillaria L.was in cycloidal or elliptic-shape.It could be concluded that the thermal stability of the starch in two species of Fritillaria L.was different due to the different structures of different starch in various plants by TGA.Conclusion The physicochemical properties of starch in two different species of Fritillaria L.differ a lot due to their geographical origin.

Acupuncture Therapy ; Adult ; Female ; Humans ; Male ; Middle Aged ; Spondylosis ; physiopathology ; therapy ; Traction ; Upper Extremity ; physiopathology

BACKGROUND:Host immune rejection is the main problem for nerve alograft in the repair of nerve defects. Therefore, how to avoid and minimize the immune rejection is the key to the success of nerve alografting. OBJECTIVE: To develop a new nerve pretreatment method by which Schwann cels and myelin can be removed from the peripheral nerve of dogs while the basilar membrane can be reserved integraly in order to obtain acelular nerve alografts. METHODS:Bilateral sciatic nerves from healthy adult dogs were taken and pretreated with the combined freeze-thaw and chemical methods folowed by microscopic observation of ultrastructural features, histological staining and western blot analysis of its ingredients. RESULTS AND CONCLUSION:Pretreated acelular nerves with good ductility and excelent epineurium toughness were empty basal lamina tubes with no Schwann cels, myelin and fragments that were al removed thoroughly, but the basilar membrane was fuly retained. These findings indicate that the optimized combination of freeze-thaw and chemical methods can efficiently clear Schwann cels and myelin which are the major antigenic components in the peripheral nerve, while preserve the basilar membrane to promote nerve regeneration. Therefore, this method can be an ideal method for preparation of tissue engineered nerves.

Betatrophin,discovered in 2013,is a protein secreted by liver,white adipose tissues,and brown adipose tissues.Betatrophin can improve glucose tolerance in mice by promoting islet β cell proliferation and increasing the number of β cells significantly and specifically.At the same time,Betatrophin is involved in lipid metabolism.Betatrophin may work as the new target for treatment of diabetes and disorders of lipid metabolism.

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Objective To investigate expression of C-MET in palliary thyroid carcinoma(PTC) detected by rapid immunohistochemical analysis and its clinical significance to predicting lymph node metastases.Methods The expression level of C-MET was examined by rapid immunohistochemical analysis with C-MET-EPOS antibody in 85 cases of PTC.Results The rate of cervical lymph node metastates in PTC with strong positive stain expression(95.0%,23/25) was significantly higher than in PTC with positive stain expression(13.4%,8/60)(P

OBJECTIVE:To establish a HPLC method for the simultaneous determination of blood drug concentration of acetaminophen and phenacetin.METHODS:Diamonil C 18 was used as the chromatographic column,the mobile phase was methanol-water(45∶55),theophyllinum was used as internal standard,the detection wavelength was254nm and the column temperature was set at the room temperature.RESULTS:The minimum determination concentration of acetaminophen and phenacetin was5?g/ml,the calibration curve was linear in a range from5～25?g/ml;The recovery rate of both acetaminophen and phenacetin in this method was more than95%;both the intraday RSD and the interday RSD were less than4.0%.CONCLUSION:The method is rapid and accurate,which can satisfy the needs of the clinic blood drug concentration deter-mination of paracetamol and phenacetin.