Objective To investigate the effects of sensorimotor therapy on patients with knee osteoarthritis.Methods Ninety-two patients with knee osteoarthritis were divided into a treatment group and a control group by using random digits table. Both groups received conventional rehabilitation therapy, including knee isometric and isotonic contraction training, ultrashort wave diathermy as well as aerobic exercise. The treatment group was also administered with sensorimotor training, in addition to the conventional training,including the muscle strengthening and control training of quadriceps and hamstring muscle using sling exercise therapy system and on the Thera-band mattress. The visual analogue scale (VAS) for pain, manual muscle testing and knee range of motion as well as the Japanese Orthopedic Association knee function evaluation form were used to evaluate the patients. Results There was no statistic difference between the 2 groups before treatment, with regard to the evaluation parameters used in this study. After 2 months of treatment, both groups improved significantly (P ＜0.05),with the treatment group improved to a significantly greater extent (P ＜0.05). Conclusions Sensorimotor training in addition to the conventional rehabilitation treatment can further relieve the pain of patients with knee osteoarthritis joints arthritis and improve the function of patients with knee osteoarthritis.

This study is to explore the amelioration of piperine on chronic acute combining stress rat with depression-like behavior, visceral sensitivity, and its effect on the expression of serotonin (5-HT) and synaptophysin. Forty two SD rats were divided into seven groups: blank group, model group, piperine (12.5, 25, 50 and 100 mgkg-1, ig) and imipramine (10 mgkg-1, ip) groups. The rat model of irritable bowel syndrome was established by chronic acute combining stress, and then to evaluate depression-like behavior and visceral sensitivity. The expressions of 5-HT and synaptophysin in the hippocampus and colon were determined by high performance liquid chromatography (HPLC) and Western blotting, respectively. The duration of immobility of IBS rat in the forced swimming test had been significantly increased, the sucrose consumption of IBS rat had been reduced and visceral sensitivity was obviously elevated in the IBS model group as compared with those in the normal control group (P<0.05, P<0.01). As compared with those in the normal control group, the expression of 5-HT significantly decreased, 5-HIAA/5-HT ratio significantly increased in the hippocampus of IBS model group (P<0.05), but opposite presentations were noted in the colon (P<0.05). As compared with that in the normal control group, the synaptophysin expression in the hippocampus decreased significantly but obviously increased in the colon (P<0.05). Piperine improved the behavior of IBS rats, and reversed the levels of 5-HT and 5-HIAA, and 5-HIAA/5-HT proportion in the hippocampus and colon (P<0.05); besides, they significantly reverse the synaptophysin level in the hippocampus and colon (P<0.05). The presence of depression and visceral sensitivity had been changed in IBS rats, with abnormal expression of 5-HT and synaptophysin in the brain-gut system. Piperine can ameliorate the changes of the behavior and regulation of serotonin and synaptophysin expression in IBS rat model.

Five compounds were isolated from marine sponge Aplysinopsis sp.collected from the South China Sea.Their structures were elucidated by ~1H-NMR,~(13)C-NMR and MS as (E)-3'-deimino-3'-oxoaplysinopsin(Ⅰ),(Z)-3'-deimino-3'-oxoaplysinopsin(Ⅱ),3-(2- oxopropyl )- 3-hydroxyind-olin-2-one(Ⅲ),1H-indole-3-carboxaldehyde(Ⅳ),5?,6?-epoxystigmasta -7-en-3?-ol(Ⅴ).CompoundsⅢ,Ⅴwere isolated from Aplysinopsis sp.for the first time.

This paper was aimed to explore the relationship between different commercial specification and chemical constituents on Radix Rehmanni,which provided experimental evidences for drawing up the commodity commercial specification and grading criteria of Radix Rehmanni.Several classified indexes,including grade,surface color,section color,section texture were carried to explore the change rules of chemical constituents by setting the contents of iridoid glycoside,sugars,water content,total ashes,acid-insoluble ashes and water extract as the markers.The results showed that the grade,surface color and section texture had less impact on chemical constituents.However,the section color had more influence.Along with the section color gradually being deepened,the contents of iridoid glycoside and oligosaccharides of Radix Rehmanni were lower,and the contents of monosaccharide and polysaccharide were higher.It was concluded that there was correlation between commercial specification and chemical constituents of Radix Rehmanni.

Objective To investigate the effects of a new sling exercise treatment combined with injections of sodium hyaluronate on the radiographic findings and clinical variables of patients with patellofemoral pain.Methods Seventy patients with patellofemoral pain syndrome were divided into a group which was treated using a new sling exercise therapy combined with injections of sodium hyaluronate (the combined therapy group,n=34) and another which received only sodium hyaluronate injections (the control group,n=36).The patients of both groups were assessed pre-and post-treatment.The clinical assessment modalities were pain scores (using a visual analogue scale,VAS),quadriceps and hamstring strength and range of motion in the active flexion and extension of the knee.Q angles were measured supine and standing.Patellofemor tilt angle and congruence and sulcus angle were determined by CT scanning.Results After 5 weeks the average VAS pain score of the combined therapy group was significantly lower than that of the control group.Muscle strength and range of motion in the combined therapy group were both significantly higher than pro-treatment,the Q angles were significantly smaller,and the congruence and sulcus angles were significantly better.Conclusion Five weeks of practicing the new sling exercise therapy combined with injections of sodium hyaluronate can yield satisfactory clinical and radiologic outcomes in patients with patellofemoral pain syndrome.

To analyze the molecular mechanisms of cross-host transmission of the Aleutian mink disease vi rus (ADV), the hypervariable region fragment of the VP2 gene of the ADV in Jilin Province (China) was amplified. Sequencing analyses showed diversity at residue 174 by comparison with other VP2 genes in GenBank. The phylogenetic tree indicated that the ADV-JL strain had a close relationship with the highly pathogenic strain from Denmark: ADV-K. Results implied that residue 174 may be associated with ADV infectivity.
Aleutian Mink Disease ; virology ; Aleutian Mink Disease Virus ; chemistry ; classification ; genetics ; isolation & purification ; Amino Acid Sequence ; Animals ; Capsid Proteins ; chemistry ; genetics ; China ; Mink ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Analysis

Objective To explore the risk factors of cognitive impairment of elderly patients with cerebral infarction in order to provide the theoretical basis for the clinical intervention. Methods A total of 237 cases with senile cerebral infarction were selected as ours subjects who were hospitalized from Mar. 2010 to Jun. 2013 in Kailuan General Hospital Affiliated to Hebei United University. The general condition and medical history were recorded. The auxiliary examination was performed. Cerebral infarction was diagnosed based on the onset to diagnosis standard and MoCA scores of within 2 weeks. The patients with less than 26 MoCA score were diagnosed as cognitive dysfunction and otherwise were thought as normal. Single factor analysis methods and non conditional Logistic regression were applied to analyze the analysis. Results There was no significant difference in terms of incidence between patients with different gender. Patients with age more than 75 years old and lower education levels had the high incidence rate than those with younger age and high education levels( χ2=16. 661,5. 453;P﹤0. 05). The cognitive dysfunction incidence of patients with white collar was lower than those with blue collar(χ2 = 5. 458,P ﹤ 0. 05 ). And the cognitive dysfunction incidence of patients with hypertention,diabetes,heart disease and leukoaraiosis were higher than those without the above diseases(χ2 =28. 423,5. 621,7. 768,6. 070;P﹤0. 05). The incidence of patients smoking more was significantly higher than that of smoking less or no(χ2 =5. 045,P ﹤0. 05 ). Multiple factors and non conditional Logistic regression analysis showed that,67 Senile cerebral infarction patients occurred cognitive impairment within 2 weeks. The independent risk factors for its occurrence included age greater than 75 years( P=0. 000 ),diabetes mellitus( P=0. 043),hypertension(P=0. 000)and leukoaraiosis(P=0. 041). Conclusion There are many risk factors related to cognitive impairment after cerebral infarction occurred in the elderly. The intervention should take in many aspects and the risk factors should early found.

Objective To evaluate the effects of intermittent nasogastric feeding on neonatal intensive care unit ( NICU) on very low birth weight infants gastrointestinal complications .Methods Two hundred and fifteen very low birth weight infants were randomly assigned to either observation group ( n=106 ) or the control group ( n=109 ) .Infants in the observation group received intermittent gravity tube feeding .Infants in the control group received continuous drip tube feeding .Weight increase , total parenteral nutrition time , length of stay and complication were assessed .Results Observation group of very low birth weight infants when were turned out of the NICU, weight increased to(1 397.0 ±22.10)g, higher than the control group (1 320.0 ± 18.25)g, was statistically significant difference (t=2.26,P<0.05);total parenteral nutrition time, length of stay in NICU were (4.94 ±0.70),(9.69 ±7.19)d,in observation group, were lower than the control group [(4.74 ±0.74),(11.63 ±4.02)d], differences were statistically significant (t=2.72,4.85,respectively;P<0.01).The incidence of abdominal distention , vomiting, diarrhea, upper gastrointestinal bleeding in observation group were lower than the control group , and had statistical significance (P<0.05).However, the very low birth weight infants had not occurred necrotizing enterocolitis in two groups .Conclusions Intermittent nasal tube feeding can reduce the complications of enteral nutrition , shorten the time of hospitalizationand increased body weight growth rate .

Objective To investigate the application effect of centralized health education among low-income parents in neonatal intensive care unit ( NICU) . Methods A total of 200 cases, who had low income parents and admitted to NICU from December 2012 to June 2013, were randomly divided into observation group (100 cases) and control group (100 cases). The neonatus in the control group underwent conventional health education mode, while neonatus in the observation group received conventional health education model based on a new type of health education on adoption. The awareness of health education, medical compliance and satisfaction of nursing assessed by self-designed health education effect scale, self-realization medical compliance and the satisfaction questionnaire in our hospital for quality of care. Results The satisfaction for quality of care in the experimental group was 97. 0% higher than 84. 0% in the control group (χ2 =9. 83,P<0. 01). The knowledge control of parents after health education in the experimental group was better than that in the control group (P<0. 01). Similarly, the medical compliance of neonatal parents in the experimental group was better than that of control group including treatment (92. 0%), treatment cooperation (95. 0%), payment on time (100. 0%) and visit as required (90.%) (χ2 =20. 37,20. 41,18. 58,14. 59, respectively;P <0. 01). Conclusions The centralized health education can facilitate the knowledge control of low-income parents in NICU, satisfaction of health education and medical compliance, and is worth of clinical promotion.

Objective To explore the application of PDCA cycle on reducing unplanned extubation ( UEX) incidence of invasive arterial blood pressure monitoring in child patients. Methods The PDCA cycle had been used to documented the UEX status of invasive arterial blood pressure monitoring in 2013, analyses the reason of UEX, set improvement goals, formulate measures, organize and implement the strategy, and compared and analyses the improved effect. Results After the introduction of PDCA cycle management approach, the experimental group of arterial indwelling catheter UEX rate (4. 5%) was lower than that of the observation group (15. 45%) (χ2 =6. 62,P <0. 05); for children of experimental group, the length of hospitalization was (22. 98 ± 2. 53) d, which was lower than that of the control group (22. 98 ± 2. 53) d (t=12. 846,P<0. 05), the expenditure of hospitalization of the control group (6 991. 1 ± 237. 1) yuan was higher than that observation group(5849.71±252.80) yuan(t=34.631,P<0.05).Conclusions ThePDCAnursingcanpreventand control the UEX rate of invasive arterial blood pressure monitoring standardize nursing operation, and improve nursing management level.