Objective To analyze the clinical feature of X-linked chronic granulomatous disease (X-CGD) and gene mutation of CYBB. Method The clinical data of X-CGD in one child and the results of CYBB gene detection in his family were reviewed. Results This boy had onset in the neonatal period and presented with recurrent severe pulmonary infection as his main manifestation. Results of nitroblue tetrazolium test (NBT) in both non-stimulation group and LPS stimulation group in the child were 0, and neutrophil oxidation index (NOI) was 1.15. Gene analysis showed a deletion mutation in exon 6 of CYBB gene in the child (579-582delATTA), which resulted in frameshift mutation started from coding sequence of 189—isoleucine (I) and stop codon occured in advance in the 212th amino acid (I189fsX212). Both the child's mother and grandmother were carriers of the mutated gene. The same deletion mutation was not found in the CYBB gene in the amniocyte from the mother's next child. Conclusion One case of X-CGD patient with CYBB gene mutation and his families were diagnosed by gene detection. Prenatal diagnosis can avoid the birth of children with X-CGD.

Local policy innovation is a distinct feature in the arena of public administration in China. Previous researches concerned with local policy innovation experience and policy introduction, but they pay little attention to the failure of policy innovations and their termination. This paper uses the theory and concept of public policy process to analyze the reasons for the quick termination process and its mechanism taking healthcare policy as a case study in Chongqing city. It concludes that relative policy strategy is closed in decision-making. The strategy of some special policy target groups as well as the mutual agitation between public and media agenda are the main reasons for the rap-id end of policy innovation. This study is helpful to understand the factors and logic mechanism of the quick termina-tion of local policy innovation as a special type of public policy change which is termed as short-lived and make more scientific healthcare policy decision making and reform in China.

Ischemic preconditioning in limb renders skeletal muscle itself or remote tissue or organ being able to resistant a subsequent more sustained ischemic insult.Its clinical application is very potent,which protects important organ such as heart,lung,brain,intestine,stomach,liver,et al.The mechanism refers to nervous or humoral factors,local or system factors,potassium channels,adenosine receptor,opioid receptor,mitochondrium.

Objective We aimed to discuss the influence of establishment of a hospital which makes people satisfied on nursing management. Methods We improved our nursing work by establishing quality-control groups, training staff, checking once a week and making feedback once a month through decomposing and subdividing the standard of building a hospital which makes people satisfied from March 2002 to December 2006. Results The qualified rate of nursing management increased from 69.3% to 97.6% every year, which has statistical difference (P<0.01). Conclusions Establishment of a hospital which makes people satisfied improved the general level of nursing management quality and nursing staff. We acquired good social and economic befenit.

Objective To study the effect of PPARγ ligand troglitazone (TGZ) on invasion and metastasis of gastric cancer cells, and investigate the relationship of PPARγ ligand with gastric cancer.Methods The expression of PPARγ in gastric cancer cell line MGC803 were detected by immunofluorescence cytochemistry method. The effect of different density TGZ on proliferation activity and adhesion of gastric cancer cell were detected by MTT chromatometry. The effect of different ligands on invasion and metastasis of gastric cancer cell MGC803 were detected by invasion system in vitro. Results The expression of PPARγ mainly located in cell nucleus. TGZ inhibited the proliferation of gastric cancer cell, decreased cell adhesion, locomotory capacity and invasion to matrigel, which had time and dose-dependent relationship.When treatment with 0. 1,1.0 and 10μ mol/ L TGZ, inhibition ratio of invasion and metastasis of cell was 8.79% ,31.31% ,51.42% and 28.29% ,4. 27% ,59. 27% respectively, which had statistical significance compared with control group( P <0. 05). When treatment was 10μ mol/L TGZ, cell adhesion was 0. 32 ±0. 03, it was statistically significant higher than that in control group (0. 52 ± 0. 04, P < 0. 05 ). Conclusion Human gastric cancer cell line MGC803 expressed functional PPARγ protein. TGZ inhibited adhesion and invasion of MGC803 cell on ECM at different degree, the effect of combination of two ligands was evident, which mechanism of action needed to be further investigated.

Objective To evaluate the effect of different densities of830nm laser irradiation on genetic damages in mice cells.Methods 100Kunming mice were randomly divided into micronuleus groups and sperm group,as well as control group.Mice thymus glands and spleens were irradiated with an830nm semiconductor laser,30mW for5minutes or20minutes each day in vitro,and the micronuclei of polychromatic erythrocytes in sternum bone marrow and rate of abnormal sperm produced were determined.Results Laser irradiation induced deformed sperm(? 2 =111.747,P=0.000),while higher dose(20min per day)laser irradiation increased the micronuclei of polychromatic erythrocytes in sternum bone marrow.There was statistical significance in the difference between the rates of abnormal sperm and micronuclei in sternum bone marrow caused by irradiation on thymus and spleen(? 2 =27.395,P=0.000)and there was dosage effect.The spleen was the more sensitive.Conclusion Laser irradiation at830nm might have a genetic damages to mice cells.Attention should be paid to the potential hazardous effects induced by semiconductor laser irradiation.

Objective To analyze the prevalence and epidemiological characteristics of chronic diseases in Liaoning Province. Methods 16 370 residents from 3 cities and 3 counties in Liaoning province were se1ected by multistage cluster sampling.A household survey with questionnaire was carried out.Chronic diseases were self-reported. Results The total prevalence rate of chronic diseases was 34.48%,and it was higher in the urban(41.85%) than that in the rural(28.65%),higher in women(39.45%)than in men(29.38%).The prevalence rate significantly increased with rising of age above 25 years old.The first five of the most prevalent chronic diseases were those of circulation system(13.18%),movement system(7.84%),digestion system(3.92%),endocrine system(2.19%) and respiration system(2.15%),and they occupied 84.87% of all the cases. Conclusion Cardiovascular diseases,hypertension and cerebrovascular diseases were the first three kinds of the most prevalent chronic diseases,and they would be the main diseases need to control and prevent.Meanwhile chronic gastritis,peptic ulcer and intervertebral disk diseases in the young people should be also concerned

Objective To investigate the short and long term effect of mifepristone on hysteromyoma.Methods The treatment group ( n=60 ) was given mifepristone and the control group ( n=60 ) vitamin C.The volume of hysteromyoma was measured using B-mode ultrasound prior to and at the end of treatment.L H,FSH,E2 progesterone ( P) ,liver function and blood creatinine were monitored each month.At the second year,3 0 cases from treatmentgroup were given mifepristone again for 3 months in the same way as the first course and with the same indexes monitored. Results Mifepristone had1 0 0 % effective rate with one-course curative rate of6.67% ,the hysteromyoma volume decreased by an average of at least 3 5.1 0 % ,while the volume gradually increased over 6months after discontinuing mifepristone with the recurrence rate of3 0 .0 0 % .At the second course of treatment hysteromyoma was further reduced by50 .2 0 % ,with the scope of reduction larger than thatatthe firstcourse( P

Objectives To analyse the predisposing cause and clinical manifestasion of diabetic ketoacidosis (DKA) complicated with multiple organic failure (MOF),in order to diagnose early and management timely.Methods 537 diabetic patients admitted in our hospital from 1997 to 2000 were included in this cases retrospective study. 182 cases of them complicated with DKA or DKA complicated with MOF (58) were further analyzed by cause, clinical manifestation, number of organs in MOF and the prognosis. Results Analysis showed that the main cause of MOF in DKA patients was infection (84 5%). The higher incidence of MOF in older DKA patients than those of younger. The possible involved organs in MOF were kidney, heart and brain etc. Involving organs of MOF were much, the mortality was closely correlated with the number of involved organs. Conclusions The possible way to reduce the incidence of MOF in DKA patients is intensive preventing and treating infection. To avoid MOF occurring in DKA patients, complications of kidney, heart and brain should be timely diagnosed and managed.