Objective To study the relationship between the OATP1B1 521T ＞ C genetic polymorphism and essential hypertension.Methods 164 essential hypertension subjects and 159 normotensive subjects were detected by the TaqMan-MGB probe real-time fluorescence quantitative PCR,and the results were compared with those of DNA sequencing.Results The frequencies of T/C genotype and C allele of OATP1B1 521T ＞ C gene of the essential hypertension subjects were obviously lower than those of the normotensive subjects(T/C genotype:0.16 vs 0.25,P ＜0.05 ;C allele:0.10 vs 0.17,P ＜0.05),The difference was significant.Binary logistic stepwise regression analysis was used for evaluatine the risk factors of essential hypertension,there was significant relationship between OATP1 B1 52IT ＞ C gene polymorphism and essential hypertension.Conclusion The SLCO1 B1 521T ＞ C variant was common in Chinese essential hypertension population,but the difference of frequency of SLCO1B1 52IT ＞ Cmuton between the essential hypertension patients and the normotensive controls was of obviously statistical significance,which indicates that the SLCO1B1521T ＞ C variant maybe associate with essential hypertension.

HIV-1 gp41 has been successfully anchored on the cell surface of yeast Saccharomyces cerevisiae by yeast cell-surface display systems using His-tag for the detection of protein expression. Gp41 activity has been detected by gp41 monoclonal antibody. The vector for gp41 yeast display has been constructed as follows: the gene-encoding gp41 was amplified by PCR using pMD18T-gp41 as a template, and then inserted into shuttle vector pICAS-His by restriction enzyme digestion. Next, the vectors were introduced into Saccharomyces cerevisiae MT8-1. After cultivation, recombinant cells were immunofluorescence labelled. The bright green cells were observed by the microscopy indicating the proteins have been displayed on the cell surface successfully, flow cytometry convinced that gp41 has been folded correctly on the cell surface. Then different concentrations of initial glucose were used to enhance the expression of protein. gp41 has been expressed by 82.46% yeast cells as the concentration of glucose was 1%. Protein expression was depressed when the concentration was increased.
Genetic Engineering ; methods ; Genetic Vectors ; genetics ; metabolism ; HIV Envelope Protein gp41 ; biosynthesis ; genetics ; Humans ; Protein Folding ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Saccharomyces cerevisiae ; cytology ; genetics ; metabolism ; Saccharomyces cerevisiae Proteins ; genetics ; metabolism ; Surface Properties

Objective:To investigate the current status of alexithymia and silence behavior in clinical nurses, and analyze the influence of clinical nurses' alexithymia on silence behavior.Methods:Using the cross sectional survey method, convenience sampling was used to select 589 clinical nurses from the First Affiliated Hospital of Wenzhou Medical University as the research object from September to October 2019. The investigation was carried out with the general information questionnaire, Toronto Alexithymia Scale (TAS) and the Employee Silence Scale to analyze the influence of alexithymia on silence behavior of clinical nurses. A total of 600 questionnaires were distributed and 589 valid questionnaires were returned, with the effective response rate of 98.2%.Results:Among 589 clinical nurses, the total scores of TAS and silence behavior were (54.07±8.86) and (36.24±6.32) respectively. The results of single factor analysis showed that in terms of job title, working years, education, department, form of employment, and marital status, there were statistically significant differences in the scores of the Employee Silence Scale in 589 clinical nurses ( P<0.05) . Pearson correlation analysis results indicated that clinical nurses' TAS scores were positively correlated with the Employee Silence Scale scores. The hierarchical regression analysis showed that describing affective disorder and extroverted thinking had a positive predictive effect on silence behavior, explaining 24.1% of the total variation. Conclusions:The alexithymia and silence behavior of clinical nurses need to be improved, and the alexithymia positively affects the silent behavior. Nursing managers should encourage clinical nurses to actively express their inner suggestions and ideas, and reduce the silence behavior of clinical nurses.