Objective To discuss application of double pipe for negative pressure drainage in reducing intra-abdominal infections after gastrointestinal operation.Methods 85 patients with intra-ahdominal drainage after gastrointestinal operation were randomly divided into the control group (43 patients) and the observation group(42 patients).The control group used traditional latex drainage,the observation group took the double cannula negative pressure continued drainage.The intra-abdominal infection and bacterial number distribution,patients drainage time,drainage volume and drainage tube occlusion were observed.Results 25 bacterial cultures were positive in two groups of patients.Compared between the two groups,the bacterial number detection rate were significant.31 pathogenic bacteria were detected in 25 specimens.Compared between these two groups,staphylococcus aureus,clearing time of drainage,drawing tube time after drainage liquid being clear,the total catheterization time,average daily drainage volume were all significantly different.Conchrsious Double pipe negative pressure drainage for postoperative abdominal drainage can effectively reduce abdominal infection,shorten drainage time,and its effect is very good.

Objective The effect of leech injection on experimental therombus formation in rats was studied. Methods The method of common carotid artery-external jugular vein by accessory pathway in rats was used. leech injection to observe its antithrombotic effect. At the same, related indecex were assessed. Results The leech injection inhibited the formation of experimental thrombus obviously and reduced aggregation of plate]et and adhesiveness of platelet. Conclusion It is suggested that leech injection has the effect of antithrombosis formation.

AIM:To investigate regulatory roles of Apelin in adventitial remodeling and fibrosis in rats with transverse aortic constriction ( TAC) .METHODS:The male Sprague-Dawley rats with TAC were randomized to daily deliver either pyroglutamyl Apelin-13 ( 50μg/kg) or saline for 4 weeks.RESULTS:Histomorphometric analysis by HE and Masson Trichrome staining revealed increased medi -al and adventitial thicknesses , especially in the adventitia , in ascending aortas in rats with TAC when compared with the sham-operated rats.Downregulation of APJ receptor and elevations in phosphorylated mTOR and ERK 1/2 levels were observed in rats with TAC . There are marked increases in heart weight ( HW) , HW/body weight ratio , and aortic fibrosis in rats with TAC .The pressure over-load-mediated pathological adventitial remodeling was strikingly rescued by Apelin-13, associated with attenuation of aortic fibrosis and reduced mRNA expression of TGF-β1, fibronectin and collagen I .CONCLUSION:Our results demonstrate the importance of Apelin-13 in amelioration of aortic adventitial remodeling and fibrosis in rats with TAC via modulation of the mTOR /ERK signaling , thus indi-cating potential therapeutic strategies by enhancing Apelin /APJ action for preventing pressure overload-and fibrosis-associated cardio-vascular disorders .

Objective To explore the effect of Tuina combined with treadmill training on nerve regeneration after sciatic nerve transection. Methods Ninty-six Sprague-Dawley rats were randomly divided into normal group (n=32), model group (n=32) and treatment group (n=32). The sciatic nerve was transected and the epineurium was sutured in the latter two groups. The treatment group was given Tuina manipulation and treadmill training once a day. Eight rats from each group were detected their sciatic nerve conduction velocity (NCV), the number of axons and Schwann cells (SCs) two, three, four and eight weeks after intervention. Results Compared with the model group, the NCV accelerated in the treatment group four and eight weeks after intervention (P<0.05); while the number of axons was significantly different two and four weeks after intervention (P<0.05), and the number of SCs was not very different between the treatment group and the model group after intervention (P>0.05). Conclusion Tuina combined with treadmill training may promote the regeneration of peripheral nerve after injury in rats.