AIM: To establish the method of fingerprint analysis of Crocus sativus L. and compare their HPLC-FPS of eight kinds of Crocus sativus L. from different sources. METHODS: HPLC with ZORBAX SB-C 18 column was used, the mobile phase was a linear gradient of methanol containing 1% acetic acid and water containing 1% acetic acid in 40min, the detection wavelength at 312nm. RESULTS: 12 marker peaks were separated. The methodological evaluation showed that the method had a good repeatability, and the marker peak area ratio of different samples were different. CONCLUSION: This method is simple and accurate with good reproducibility and can be used for a quality control for Crocus sativus L.

Objective To explore the clinical effect of laparoscopic uterine artery occlusion combined with myomectomy on treatment of patients with uterine fibroids.Methods 112 patients with uterine fibroids were randomly divided into the observation group (n=56)and the control group (n=56),the observation group was treated with laparoscopic uterine artery occlusion com-bined with myomectomy,while the control group was treated with laparoscopic myomectomy only. Clinical effect was contrasted between the two groups.Results In the observation group,operative time and hospital stay were significantly shorter than the control group(P <0.01),blood loss was less than the control group(P <0.01),and the exhaust time was significantly earlier than the con-trol group(P <0.05).In the observation group,remission rate of menorrhagia was 96.4%,which was significantly higher than 80.4% in the control group(P <0.01).Uterine fibroid recurrence rate was 1.8%,which was significantly lower than 16.1% in the control group(P <0.01),but degree of uterine volume reduction was significantly higher than the control group(P <0.01). There was no significant difference of incidence of postoperative complications between the two groups.Conclusion Laparoscopic uterine artery occlusion combined with myomectomy can effec-tively reduce intraoperative loss of blood,improve therapeutic efficacy and reduce incidence rate of complications and recurrence rate.

Objective To explore the clinical effect of laparoscopic uterine artery occlusion combined with myomectomy on treatment of patients with uterine fibroids.Methods 112 patients with uterine fibroids were randomly divided into the observation group (n=56)and the control group (n=56),the observation group was treated with laparoscopic uterine artery occlusion com-bined with myomectomy,while the control group was treated with laparoscopic myomectomy only. Clinical effect was contrasted between the two groups.Results In the observation group,operative time and hospital stay were significantly shorter than the control group(P <0.01),blood loss was less than the control group(P <0.01),and the exhaust time was significantly earlier than the con-trol group(P <0.05).In the observation group,remission rate of menorrhagia was 96.4%,which was significantly higher than 80.4% in the control group(P <0.01).Uterine fibroid recurrence rate was 1.8%,which was significantly lower than 16.1% in the control group(P <0.01),but degree of uterine volume reduction was significantly higher than the control group(P <0.01). There was no significant difference of incidence of postoperative complications between the two groups.Conclusion Laparoscopic uterine artery occlusion combined with myomectomy can effec-tively reduce intraoperative loss of blood,improve therapeutic efficacy and reduce incidence rate of complications and recurrence rate.