1.Extended profundaplasty for lower extremity ischemia
Shuixian QIAN ; Shenglai ZHANG
Chinese Journal of General Surgery 1993;0(02):-
Objective To evaluate the clinical effectiveness of extended profundaplasty for treatment of critical leg ischemia. Methods Twenty-five legs (stage Ⅲ and Ⅳ in Fontaine′s classification) of 23 patients with critical leg ischemia caused by extensive occlusion of the distal arteries were reviewed during past 12 years. All patients underwent extended profundaplasty and were evaluated by clinical observation of API, tcPO 2, PSaO 2, Treadmill test, NIRS and ultrasound perioperatively. Results Fourteen legs in 13 patients had a favorable outcome, seven legs in 6 patients had an improvement on their symptom and sign, three legs in 3 patients underwent below knee and one case medio-tarsal amputation during the periods of 7 months to 7 years follow-up. Conclusion The extended profundaplasty is simple, less invasive, safe and effective for the treatment of limb-threatening ischemia.
2.The study of the correlation of pregnancy -induced hypertension in the case of indexing syndrome and preg-nancy outcome
Chinese Journal of Primary Medicine and Pharmacy 2015;(22):3372-3375
Objective To study the correlation of pregnancy -induced hypertension in the case of indexing syndrome and pregnancy outcome,and to explore their clinical applicability.Methods 30 cases of birth mothers with pregnancy -induced hypertension were selected.According to the severity of hypertension,the patients were divided into the severe pregnancy -induced hypertension group,the moderate pregnancy -induced hypertension and the mild gestational hypertension group.The different levels of pregnancy -induced hypertension maternal outcome indicators, and different maternal outcomes in the two groups were analyzed and compared.Results Preterm birth (58.3%), cesarean section (66.7%),gestational age (33.6 ±2.8)weeks,baby weight (2 330.3 ±652.3)g of pregnancy -in-duced hypertension group were significantly higher than moderate pregnancy -induced hypertension group[18.2%, 18.2%,(36.6 ±3.7)weeks and (2 892.6 ±761.4)g],the differences were statistically significant (χ2 =3.884 5,χ2 =5.490 0,t =2.204 8,t =1.907 1,all P <0.05).The eclampsia (41.7%),postpartum hemorrhage (58.36%), acute renal failure (41.7%)of pregnancy -induced hypertension group were significantly less than the moderate (9.1%,18.2%,9.1%),mild maternal gestational hypertension group (0.0%,14.3%,0.0%),the differences were statistically significant(χ2 =3.158 6,χ2 =3.884 5,χ2 =3.158 6,all P <0.05).Those of moderate maternal gestational hypertension group were significantly higher than the mild gestational hypertension group,the differences were statistically significant (P <0.05).Conclusion Pregnancy -induced hypertension syndrome has significant effect on pregnancy outcomes,the severity degree of pregnancy -induced hypertension is positively correlated with pregnancy outcomes,so early prevention of pregnancy -induced hypertension can increase overall maternal prognosis.
3.Mini-incision surgery for the treatment of venous varicosis of lower limbs
Shuixian QIAN ; Jianjun WANG ; Shenglai ZHANG
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To evaluate the therapeutic effect of mini-incision surgery for the treatment of varicose vein of lower extremities. Methods 106 limbs of 95 paitnets with varicose vein underwent saphenectomy combined with ligation of communicating veins by way of mini-incision from November 1997 to April 2001. The ascending venography and StethoDoppler examination were performed preoperatively in all of the patients. Results Venous reflux in deep vein of lower extremities were at grade 0 in 62 limbs, at grade Ⅰ in 28 limbs and at grade Ⅱ in 16 limbs demonstrated by ascending venography with Valsalva test. StethoDoppler examination showed that valve insufficiency of great saphenous vein at saphenofemoral junction was in 99 limbs, the insufficiency of small saphenous vein at saphenopopliteal in 5 limbs and incompetent perforating vein in all of the limbs. Postoperative course was uneventful, the average hospitalization days after operation were 2.8 days and no recurrent varicose veins occurred in all the patients during the follow-up period lasting 1-30 months. Conclusions With the help of preoperative StehoDoppler examination, the mini-incision surgery for the treatment of varicose vein of lower extremities is safe, simple, effective and lower costs.
4.Genetic analysis of neonatal diabetes mellitus and its influence on treatment outcome
Xiuli CHEN ; Shuixian SHEN ; Feihong LUO ; Miaoying ZHANG ; Tang LI ; Linqi CHEN ; Min HU ; Hong DU ; Lingfeng CAO ; Ruoqian CHENG ; Zhuhui ZHAO ; Dijing ZHI
Chinese Journal of Endocrinology and Metabolism 2011;27(6):488-491
Fourteen neonatal diabetes mellitus(NDM)patients were recruited. 9 patients were treated with glyburide and the other 5 with insulin. ABCC8, KCNJ11, and INS genes were sequenced in 6 of them. Gene mutations were found in 2, 1, and 1 cases in these genes, respectively. One case with 6q24 hypomethylation and another without known mutation were also found. 8 out of 9 patients treated with glyburide reached euglycemia(88.9%). The other 5 patients with insulin therapy either died or lost contact. The results suggest that glyburide therapy is effective in neonatal diabetes mellitus, while insulin therapy may contribute to poor compliance.
5.Influence of group health care model during pregnancy on midwifery based primipara′s self-efficacy and quality of life
Shuixian ZHANG ; Lin ZHOU ; Liangfang SHI ; Xia LEI
Chinese Journal of Health Management 2021;15(5):459-463
Objective:To explore the influence of a midwife-led group health care model on primiparas′ self-efficacy and quality of life.Methods:The study included 200 primiparas who registered with the obstetrics department of our hospital between January and December 2019. The puerperas were divided into two groups with 100 cases each according to the random number table method. The control group received routine health care during pregnancy. The T-test and χ 2 test were used, while the observation group received midwifery-led group health care during pregnancy. The scores of maternal role adaptability, self-efficacy, weight control, delivery mode, breastfeeding, mental state, and quality of life was compared between the two groups. Results:After nursing, the mother′s role adaptability (63.14±9.38) and self-efficacy (34.16±2.89) scores in the observation group were higher than those in the control group (52.89±8.41 and 31.28±2.43, respectively) ( t=8.136, 7.627, P<0.05). The weight gain of the observation group in the third trimester [(3.68±1.22) kg] was less than that of the control group [(5.49±1.76) kg] ( t=8.452, P<0.05). The vaginal natural delivery rate of the observation group was 91% higher than the 71% of the control group (χ2=12.966, P<0.05), the cesarean section rate of the observation group was 9% lower than the 29% of the control group (χ2=12.966, P<0.05), and the exclusive breastfeeding rate in the observation group was higher than that in the control group ( χ2 =6.258, P<0.05). After nursing, the SAS (40.74±4.93) and SDS scores (42.16±5.07) of the observation group were lower than those of the control group (47.10±5.27 and 48.59±5.42, respectively) ( t=8.813, 8.664, P<0.05), and the four-dimension quality of life scores of the observation group were higher than those of the control group ( t=7.293, 7.406, 7.357, 8.767, P<0.05). Conclusion:A midwife-led group pregnancy care model can effectively enhance the self-efficacy of primiparas and improve their quality of life.
6.Practice effects of midwife-centered antenatal care model in family groups
Lin ZHOU ; Dandan CHEN ; Yimin LI ; Zimei CHEN ; Shuixian ZHANG
Chinese Journal of Modern Nursing 2019;25(5):539-542
Objective? To explore the effects of the midwife-centered antenatal care model attended by pregnant women on reducing cesarean delivery rates and properly controlling neonatal weight. Methods? Totally 120 pregnant women who established medical records and attended antenatal care regularly in Hangzhou First People's Hospital from February 2017 to January 2018 were selected by on-site recruiting and divided into the treatment group (n=60) and the control group (n=60) according to the random number table. Pregnant women in the control group received conventional antenatal care, while pregnant women in the treatment group received the midwife-centered antenatal care in family groups. The cesarean delivery rate, postpartum hemorrhage rate, neonatal weight, incidence rate of fetal macrosomia and incidence rate of low birth weight infant were compared between the two groups. Results? The cesarean delivery rate of the treatment rate was 8.3%, while that of the control group was 35.0% (χ2=12.570, P<0.01); the neonatal weight of the treatment group was (3.22±0.39) kg, while that of the control group was (3.51±0.47) kg (t=-3.70,P<0.01). Conclusions? The midwife-centered antenatal care in family groups can reduce cesarean delivery rates and properly control neonatal weight, which is of significance in nursing research and clinical nursing work.
7.The role and prospect of 4I training mode in the interdisciplinary talent training of life sciences in universities
Qian ZHANG ; Xuyang ZHANG ; Dan LIU ; Shuixian ZHANG ; Rong HU
Chinese Journal of Medical Education Research 2021;20(5):557-560
The cultivation of interdisciplinary talents for life science is still in the state of exploration. The 4I training mode includes four key elements, interdisciplinary course setting, integration of cross project, instruction and administration across whole research and innovational evaluation at the end. Based on the 4I training mode, systematic and targeted design and arrangement would enhance the training level of interdisciplinary talents of life science in universities.
8.A cohort study on the impacts of pre-pregnancy maternal body mass index,gestational weight gain on neonate birth status and perinatal outcomes in Fujian province
Yanhua LI ; Xiaomei CHEN ; Shuixian CHEN ; Jiangnan WU ; Xiuyun ZHUO ; Qiaoling ZHENG ; Xiuqing WEI ; Ronghua ZHANG ; Huiqing HUANG ; Cuixian ZHENG ; Juan LIN
Chinese Journal of Epidemiology 2014;(6):635-640
Objective To study the impacts of pre-pregnancy maternal BMI and gestational weight gain(GWG)on pregnancy outcomes. Methods We adopted a prospective cohort study with cluster sampling in single pregnant women,who were not with hypertension,diabetes,hyperlipidemia or other diseases in the previous history,neither did they have diseases of heart,liver,kidney,thyroid etc. related to current pregnancy. Those pregnant women who visited the prenatal nutrition clinic under‘informed consent’were surveyed with questionnaire to track their peri-natal complications,delivery mode and neonate birth outcomes etc. Pearson and partial correlations,chi-square test and binary logistic regression were used to study the association between pre-pregnancy maternal BMI,GWG and pregnancy outcomes. Results A total of 623 pregnant women were recruited in the cohort,with 592(95%)of them eligible for analysis. Results from the Multivariate Logistic Regression analysis indicated that,after controlling the potential confounding factors,when compared to women with pre-pregnancy BMI between 18.5 and 24.0,the odds ratios(ORs)for low birth ponderal index(PI) were 2.34[95%confidence interval(CI),1.24-4.42)]among those with BMI<18.5,respectively,while 2.73(1.12-6.68)for high birth PI among those with BMI>24.0. Similarly,when compared to pregnant women with normal GWG(defined as weight gain range from P15 to P85 by stratification of pre-pregnancy BMI),low GWG(
9.Relying on the SPOC platform to flipping medical immunology classroom teaching
Hui PANG ; Shuixian LI ; Jinsheng WANG ; Ying ZHANG ; Li LI
Chinese Journal of Medical Education Research 2018;17(12):1219-1223
Objective To explore the application and effect of SPOC platform in medical immunol-ogy teaching. Methods The teaching reform class of Grade 2015 (41students) of Changzhi Medical College was taken as the experimental group, while the parallel class of imaging (37 students), preventive specialty (40 students), Class Nine of clinical medicine (37 students) and oral specialty (39 students) were randomly selected as the control group. "SPOC learning platform + traditional teaching" method was used for teach-ing and learning in the course of the test group. The traditional teaching method was utilized in the control one. For this type of teaching activity, a questionnaire survey was carried out among the students in the reform class, and the final exam score was used as the measure standard. The data were analyzed by SPSS 17.0 software. Results The results demonstrated that the students preferred to learn on the SPOC platform for mobile phones, and the number was significantly higher than that of learning through the computers. Students in the reform class basically liked this type of learning, and their final exam scores were more significant difference than the other classes in the same grade (t'=15.21, P=0.000). The classes in the experimental group and the control group were taken as the research objects, and the rank-sum test was performed, which was statistically significant ( χ2=81.65, P=0.000). Then, the two groups were compared in different classes: the statistical difference between the teaching reform class and image 1502 (t'=11.52, P=0.000), prevention 1502 (t'=7.38, P=0.000), clinical medicine 1509 (t'=8.93, P=0.000), oral 1501 class (t'=8.93, P=0.000) were found. The questionnaire survey demonstrated that 90.24% (37 students) of the reform class liked this form of learning and at the same time, it was suggested that the learning content on the platform should be further linked to the clinical practice and combined with scientific research to expand the students' thinking. Conclusion The SPOC learning platform has turned over the teaching of medical immunology and improved students' motivation and interests in learning.
10.Effect of long non-coding RNA-ROR mediating epithelial-mesenchymal transformation on radiotherapy resistance of nasopharyngeal carcinoma cells in vitro
Xiaocheng XUE ; Xue ZHANG ; Shuixian HUANG ; Yi ZHANG ; Dan LU ; Xiaoping CHEN
Academic Journal of Naval Medical University 2024;45(10):1218-1225
Objective To investigate the role of long non-coding RNA(lncRNA)-ROR in mediating epithelial-mesenchymal transformation(EMT)and its impact on radiotherapy resistance in nasopharyngeal carcinoma cells.Methods Nasopharyngeal carcinoma cells CNE2 were divided into blank group,negative control(NC)group and lncRNA-ROR siliencing group;or were divided into blank group,radiotherapy group,radiotherapy+NC group,and radiotherapy+lncRNA-ROR overexpression group(radiotherapy treated with 6 Gy radiation for 24 h).The CNE2 proliferation was detected by cell counting kit 8 method.The cell migration was detected by cell scratch test and Transwell cell migration test.The apoptosis ratio was detected by flow cytometry,and the apoptosis-related proteins and epithelial-mesenchymal transition proteins were detected by Western blotting.Results Compared with the blank group and NC group,the proliferation ability of nasopharyngeal carcinoma cells CNE2 was decreased after inhibition of lncRNA-ROR expression for 48 and 72 h(all P<0.05).The mobility of CNE2 cells after lncRNA-ROR expression inhibition was lower than that in the NC group(P<0.05).The migration ability of CNE2 cells in the radiotherapy+lncRNA-ROR overexpression group was higher than that in the radiotherapy group and radiotherapy+NC group(both P<0.05).Compared with the radiotherapy group and radiotherapy+NC group,the apoptosis rates of CNE2 cells in the radiotherapy+lncRNA-ROR overexpression group was decreased(both P<0.05).After lncRNA-ROR inhibition,the expression of activated caspase 3 and caspase 9 proteins was increased(both P<0.05),while the expression of activated caspase 3 and caspase 9 proteins was decreased in the radiotherapy+overexpressed lncRNA-ROR group(both P<0.05).Inhibition of lncRNA-ROR increased the expression of epithelial marker proteins(E-cadherin,β-catenin),and decreased the expression of interstitial marker proteins(N-cadherin,vimentin).The epithelial marker protein expression was decreased and interstitial marker protein expression was increased in CNE2 cells in the radiotherapy+lncRNA-ROR overexpression group compared with the radiotherapy group and radiotherapy+NC group(all P<0.05).Conclusion lncRNA-ROR can affect the radiotherapy resistance of nasopharyngeal carcinoma cells by regulating their proliferation,migration,apoptosis and EMT,and it is a potential target for reversing the radiotherapy resistance of nasopharyngeal carcinoma cells.