Objective: To select the optimum alcohol concentration of Shangtong Tincture. Methods: The percutaneous absorption test was performed on excised rats skin in improved Franz diffusion cell. The penetrative amount of paeonol was determined quantitatively by HPLC and the content of paeonol as a marker. Results: The penetrative amount of paeonol of Shangtong Tincture with 60%～80% alcohol concentration have a optimal value, the average rate is 0.010mg/cm 2?h. Conclusion: Shangtong Tincture with 70% alcohol concentration is the best.

AIM: To provide a HPLC fingerprint of Fructus aurantii micropowder in order to create a basis for(identification.) METHODS: With the help of computer similarity evaluation,Seventeen kinds of Fructus aruantii sample were pulverized and micronized separately,then were extracted by methanol and compared correlation between both extracts. RESULTS: In methanol extraction,contents of aurantiamarin,hesperidin and neohesperidin in micropowder were more than that in pulverized powders,correlation of both HPLC fingerprint was 0.9~1.0. CONCLUSION: The method established can be used for identifying and evaluating crud drug of Fructus aurantii,and further prove that micronization is beneficial to increasing flavonoid dissolution.

Objective: To provide experiment data for ultra fine prepared mineral drugs Methods: Electron microscope scannin,X ray diffraction were used in identification, atomic emit sectrum was used in determination. Results: The dissolution rate of ca 2+ composition could be high.The ultra fine prepared of minaral drugs could be prepared with powder diameter of K 4。 Conclusion: Ultra fine Prepared fluoritum and gypsum fibrosum may Save clinically dose.

AIM: To provide application and identification basis for methanol-extratis of Fructus aurantii by constructing the HPLC-FP. METHODS: The C_(18) column was used with a mobile phase of(A)(0.095%) phosphoric acid acetonitrile-(B)(0.095%) phosphoric acid gradient elution.The flow rate was(1.0) mL/min.The wavelength of detecter was set at 330 nm.Naringin was reference standard. RESULTS: By cluster analysis,the eighteen kinds of Fructus aurantii samples were classified as four clusters: the superior in producing area,the ordinary in producing area,the ordinary and the inferior.By similarity calculation,the similarity of the superior in producing area and the ordinary in producing area were(0.9)～(1.0),and the ordinary and the inferior were less than(0.9). CONCLUSION: The HPLC-FP of Fructus aurantii has been established.The method can be used to identify and evaluate the quality of Fructus aurantii.

AIM: To investigate the influence of microwave-assisted extraction on flavone contents of Pollen Tyhae micropowder. METHODS: Ultraviolet spectrophotometer and HPLC were applied to analyze Pollen Tyhae micropowder, total flavon and isorhamnetin-3-O-neohespridoside were adopted as the marker, respectively. RESULTS: Appropriate conditions of microwave-assisted extraction included: extraction time of 8min, ethanol concentration of 70%, Solid/liquid ratio of 1∶18 (g?mL -1) and the power of microwave oven of 540w. CONCLUSION: Compared with normal reflux method, microwave-assisted extraction of Pollen Typhae micropowder is more useful and can improve the extraction rate the reduce the extracting time.

OBJECTIVETo establish the double HPLC fingerprints of water-soluble composition and amino acids precolumn derivative reagent of 13 batches of Isatidis Radix micropower.

METHODThe gradient elution was adopted with Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm). Water-soluble ingredients were detected with acetonitrile-0.1% phosphoric acid solution as mobile phase, flow rate 0.5 mL x min(-1), column temperature 20 degrees C, and the injection volume 10 microL. Amino acid ingredient were derived by PITC, and then were detected with mobile phase of 0.1 mol x L(-1) sodium acetate buffer solution (pH 6.5) - acetonitrile, flow rate 1.0 mL x min(-1), column temperature 30 degrees C, and the injection volume 5 microL. Both of the absorption wavelengths were 254 nm. Pharmacopoeia Commission "Chinese chromatographic fingerprint evaluation system (version 2.0)" was adopted to analyse, fingerprints of Isatidis Radix micropower were established, at the same time 4 main ingredients were recognized by the SPSS cluster analysis.

RESULTCommon mode of Fingerprint to water-soluble and amino acids ingredient of Isatidis Radix micropower was established, then adenosine, epigoitrin and 15 amino acids were identified as characteristic peaks. Cluster analysis showed that different kinds of the herbal Isatidis Radix micropower from different areas were different levels in the main ingredients.

CONCLUSIONDouble fingerprints of Isatidis Radix micropower is established. Each peak is optimally separated in chromatogram, which provides a scientific basis for quality control of Isatidis Radix micropower.

Objective:To establish a high-performance liquid chromatography (HPLC) characteristic spectrum of Radix Actinidiae Chinensis decoction pieces, standard decoction and formula granules; To simultaneously determine the contents of index components; To study the transfer law of formula granules based on the extraction amount, content of Catechin and characteristic spectrum of standard decoction.Methods:15 batches of Radix Actinidiae Chinensis decoction pieces were collected, the standard decoction was prepared, and the extraction amount was measured. The HPLC fingerprints of decoction pieces, standard decoction and formula granules were established, and the characteristic peaks were calibrated and attributed. The content of Catechins in decoction pieces, standard decoction and formula granules was measured, and the transfer rate law was calculated. The yield rate, the common peak transmission number of fingerprints, the content and the transfer rate were the main evaluation indexes, and the law of the transmission of the magnitude value was analyzed.Results:The paste yield of 15 batches of Radix Actinidiae Chinensis standard decoction ranged from 3.9%-6.3%. A total of 4 characteristic peaks were calibrated in the feature map, and peak 2 was identified as Protocatechuic acid and peak 4 as Catechin; 6 characteristic peaks were detected in Actinidia chinensis Planchon decoction pieces, standard decoction and formula granules, and their relative retention times were all within the specified range. The content of Catechins in Radix Actinidiae Chinensis decoction pieces was 0.4%-1.4%, which was 3.8%-4.9% in formula granules. The transfer rate of decoction pieces-standard decoction was 13.6%-38.3%, and the decoction-formula granules was 15.5%-21.2%.Conclusions:The mass transfer between Radix Actinidiae Chinensis decoction pieces, standard decoction and formula granules has a good migration. The formula granules also have a good correlation and consistency with the standard decoction, which indicating that the preparation process of Radix Actinidiae Chinensis is reasonable and feasible.

Objective To analyze the volatile constituents of Linglingxiang (Lysimachia foenumgraecum,Hance,Holy Basil) by applying solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC/MS).Methods The heads of solid phase microextraction (SPME) with 4 kinds of different coating,75 μm CAR/PDMS,65 μm DVB/PDMS,85 μm PA and 100 μm PDMS,were used to extract the under the best extraction condition,and then these volatile constituents were detected by using gas chromatography-mass spectrometry (GC-MS).Results The key parameters of SPME for extracting volatile constituents from Linglingxiang were as follows:temperature was 85℃C and extracting time was for 50 min.A total of 10 types including 103 kinds of volatile constituents were identified by 4 different SPME extraction heads,and phenols,esters and hydrocarbons were main chemical types of volatile compounds in Linglingxiang.There were totally 6 volatile constituents detected by 4 different SPME extraction heads,and they were Sugarlactone,Dihydroactinidiolide,(+)-cedrol,phytone,phenanthrene,and 3-Amino-4,5-dimethyl-2 (5h)-furanone.Among all extraction heads,the head of 75 μm CAR/PDMS detected the largest number of volatile constituents reached to 43.Conclusion SPME-GC/MS was used for the first time form determining the volatile constituents in Linglingxiang.The method is simple,rapid and accurate,which can offer reference to further studies on the material base of Linglingxiang.

Objective To predict the quality marker(Q-marker)of Wuwei disinfection decoction based on HPLC fingerprints and network pharmacology.Methods Using the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 version)software,the fingerprints of 15 batches of Wuwei disinfection decoction were established,and the peaks were found and assigned to each batch.Wuwei disinfection decoction's primary targets and routes were screened using network pharmacology,and a"component-target-pathway"network diagram was established.The five principles of the Q-marker were used to predict the quality marker in Wuwei disinfection decoction.Results The similarity of the fingerprints of 15 batches of Wuwei disinfection decoction was＞0.95,13 peaks were identified,and 11 peaks were recognized as neochlorogenic acid,chlorogenic acid,esculetin,cryptochlorogenic acid,loganin,secoxyloganin,chicoric acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C and linarin.Through the pharmacological analysis of the network,it was speculated that chlorogenic acid,esculetin,secoxyloganin,chicory acid,and linarin might be potential Q-marker of Wuwei disinfection decoction,which may regulate multiple genes,such as tumou suppressor p53/oncoprotein Mdm2,tyrosine-protein kinase SRC,as well as multiple cancer pathways such as pancreatic cancer and prostate cancer,to produce anti-tumor and anti-cancer effects.At the same time,Wuwei disinfection decoction was also predicted to be used for the treatment of COVID-19.Conclusion The established HPLC fingerprinting method of Wuwei disinfection decoction was sensitive,rapid,and stable.Based on the network pharmacology results,potential Q-marker with traceability,predictability,and close relevance to the clinical application of Wuwei disinfection decoction was screened,which can provide a reference for further research on Wuwei disinfection decoction in the future.

Through the textual research and analysis of the variety， origin， processing， quality evaluation and clinical application of Moslae Herba in ancient and modern literature， its origin of materia medica was clarified. Moslae Herba has experienced variety changes in history. Elsholtzia ciliata was the mainstream variety during and before the Song dynasty， however， during the Ming and Qing dynasties， emerging variety of Mosla chinensis rose to the mainstream status due to its remarkable efficacy and the formation of cultivation， and differentiated into two commodities（wild variety of Qingxiangru and cultivated variety of Jiangxiangru）， cultivated products formed an authentic producing area in Jiangxi. The three varieties coexisted during the Ming and Qing dynasties， and the Elsholtzia varieties were gradually eliminated. Variety changes have caused changes in the functions and indications of drugs. E. ciliata had the effect of clearing heat and was mainly used to treat heatstroke and cholera， while M. chinensis was used for exogenous wind cold and dampness in the summer because of its warm and strong sweating properties， but not for cholera. Traditional Moslae Herba is mainly harvested in the summer and autumn （flowering to fruiting stage） and the above-ground parts are dry in the shade and used as medicine. Modern Qingxiangru is mostly harvested before the flowering period， and Jiangxiangru is harvested after flowering and fruiting in late summer and early autumn. In summary， according to the 2020 edition of Chinese Pharmacopoeia， the dried above-ground parts of Moslae Herba should be selected for Xinjia Xiangruyin in the Catalogue of Ancient Famous Classical Formulas（The First Batch）， mainly the cultivated variety of Jiangxiangru， and the raw products is cut into segments and used as medicine. It is suggested that when applying and developing famous classical formulas containing Moslae Herba at different periods of time today， the origin should be established in conjunction with clinical efficacy.