1.Effect of personalized intervention on elderly patients with colostomy after Miles operation for rectal carcinoma
Ju ZHANG ; Chunrong LIU ; Fang SHUI ; Qian HE ; Yanling XIAO
China Journal of Endoscopy 2017;23(7):54-59
Objective To study the clinical effect of personalized intervention on elderly patients with colostomy after Miles operation for rectal carcinoma. Methods 114 cases elderly patients with colonic stoma after Miles operation for rectal cancer from June 2014 to January 2016 were divided into control group and observation group by random number method, 57 cases in each. The control group were treated with routine intervention, while patients in observation group was treated with personalized intervention. the self-care ability score before intervention, the incidence of complications, intervention satisfaction and life quality score were compared between the two groups at the same time. Results The total complication rate in the observation group was significantly lower than that in control group ( 5.25% vs 22.80%) (χ2 = 8.36, P = 0.000); the intervention satisfaction of the observation group was significantly higher than that in control group (91.23% vs 75.44%) (χ2 = 6.60, P = 0.010). After intervention, the self-care ability score and life quality score of observation group were significantly higher than that in control group (P < 0.01). Conclusion The personalized intervention can reduce the complications, significantly improve the patients' life quality score and self-care ability, and effectively alleviate the negative situation, improve the patients intervention satisfaction, with a higher development value, it is worth of clinical promoting.
2.Expression and identification of VEGF165 in bone marrow mesenchymal stem cells of rhesus
Zaiyu GUO ; Heliang ZHANG ; Tao SHUI ; Guozhe ZHANG ; Weihua ZHAO ; Qian CHEN ; Yanwei HOU
Tianjin Medical Journal 2016;44(10):1209-1212
Objective To detect the transferred vascular endothelial growth factor (VEGF)165 gene expression in rhesus autologous bone marrow mesenchymal stem cells (MSCs), and to explore the functional viability of transgenic MSCs. Methods MSCs from rhesus bone were isolated by Ficoll, which were used to detect the phenotype. After the culturing, the expression vector pcDNA-eGFP-VEGF165 was transfected into bone marrow MSCs. Fluorescence microscope and flow cytometry were used to detect the enhanced green fluorescent protein (eGFP) expression. At the same time, the phenotype in transfected MSCs was also indentified. The VEGF165 expression level was detected by RT-PCR. Results The highly purified MSCs were collected successfully. The transfected MSCs and daughter cells showed expressions of eGFP and VEGF165, which also remained the characteristics of MSCs. Conclusion The VEGF165 gene that is transfected into MSCs can maintain characteristics of MSCs, and stably express foreign genes.
3.Cloning, expression and immunity of pilA gene and ompC gene from avian pathogenic Escherichia coli.
Shan YU ; Qian ZHANG ; Xiaoxi SHUI ; Zhouliang YU ; Baohua ZHAO
Chinese Journal of Biotechnology 2008;24(9):1561-1567
In order to amplify pilA gene and ompC gene of avian pathogenic Escherichia coli (APEC) strain, two pairs of primers were designed according to the GenBank sequences, and a 549 bp pilA gene and a 1104 bp ompC gene were obtained by PCR separately. Sequence analysis indicated that the homology of the nucleotide sequence of AEPC strain to those other reference strains was 98.18% of the pilA gene and 97.28% of the ompC gene. Two expression plasmids pETpilA and pETompC were constructed by inserting pilA gene and ompC gene into the prokaryotic expression vector pET-28a. The two plasmids were transformated into E. coli BL21 separately and two recombinant strains BL21 (pETpilA) and BL21 (pETompC) were obtained. The type 1 fimbraie and the out membrane protein were highly expressed when the recombinant strain BL21 (pETpilA) and BL21 (pETompC) were induced by IPTG Two specific proteins were detected by SDS-PAGE and immunogenicity of the expressed protein was confirmed by Western blotting and ELISA. The expressed fimbraie and OmpC were transformed into vaccine. The protective immune response was proved after the mice were immunized with the two vaccines. The results showed that the recombinant strain BL21 (pETpilA) and BL21 (pETompC) could be as candidate vaccine to provide protective immune response against AEPC infection.
Animals
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Cloning, Molecular
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Escherichia coli
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genetics
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immunology
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metabolism
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Escherichia coli Proteins
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genetics
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immunology
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metabolism
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Escherichia coli Vaccines
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immunology
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Fimbriae Proteins
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genetics
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immunology
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metabolism
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Gene Expression Regulation, Bacterial
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Genes, Bacterial
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Mice
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Porins
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genetics
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immunology
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metabolism
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Recombinant Fusion Proteins
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genetics
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immunology
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metabolism
4.Treatment of desmoid tumour in head and neck.
Yong-Xue ZHU ; Shui-Zhang QIAN ; Ling ZHANG ; Yi WU ; Qing-Hai JI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(6):432-434
OBJECTIVETo find out the suitable therapy for the patient of desmoid tumour in head and neck.
METHODSForty-four patients with desmoid tumours of the head and neck treated at Cancer Hospital of Fudan University between 1987 and 2002 were identified from inpatient tumour database. Patients were classified into three groups: operation group (15 cases); operation + radiation group (12 cases); radiation group (17 cases). All patients were prospectively followed. Clinicopathologic features and treatment modalities were evaluated.
RESULTSIn the group of operation, four of operation group had recurrences 26.7% (4/15). In the group of radiation, three of radiation group had recurrences 17.6% (3/17). And the recurrence of operation + radiation group was 23.5% (4/17). No patient died of their disease.
CONCLUSIONFor desmoid tumors of the head neck, operation + radiation was recommended, and the benefits of radiation therapy were demonstrated.
Adolescent ; Adult ; Aged ; Combined Modality Therapy ; Female ; Fibromatosis, Aggressive ; surgery ; therapy ; Head and Neck Neoplasms ; surgery ; therapy ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult
5.Development and application of a mammlian one hybrid-based high-throughput screening model for Eralpha modulator.
Qian ZHANG ; Xiaoxi SHUI ; Yuling FAN ; Weili HAO ; Zhihui ZHENG ; Xinhua LU ; Baohua ZHAO ; Hua ZHANG ; Jiangong HE
Chinese Journal of Biotechnology 2009;25(7):1088-1094
Estrogen Receptor (ERalpha) is a member of superfamily of ligand-activated transcription factors which play critical roles in many biological processes. To screen novel modulators of ERalpha for drug development and biological function research, we developed a mammalian one-hybrid-based high-throughput screening model for ERalpha modulator. We cloned the ERalpha LBD gene from the total mRNA of fat tissue by RT-PCR and fused it with the GAL4 DNA binding domain of pBIND-GAL4 plasmid to construct a chimara expression plasmid pBIND-GAL4-Eralpha(LBD). The L02 cells was cotransfected with pBIND-GAL4-ERalpha(LBD) and a GAL4-responsive luciferase reporter plasmid pGL3-GAL4, and following treatment with test compounds for 24 h, the activities of luciferase were detected to evaluate the transactivities of ERalpha modulators. After manner optimizations of transfection conditions, Estradiol, an agonist control, induced the expression of luciferase in a dose-dependent with EC50 of 0.17 micromol/L, the maximum folds of induction was about 28.1. Tamoxifen, an antagonist control, efficiently suppressed the estradiol-mediated luciferase induction with EC50 of 0.10 micromol/L. Using this screening model, we discovered four ERalpha agonists from 2000 natural and synthetic compounds.
3T3-L1 Cells
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Animals
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Chimera
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metabolism
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DNA-Binding Proteins
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biosynthesis
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genetics
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Estrogen Receptor Modulators
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chemistry
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isolation & purification
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Estrogen Receptor alpha
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agonists
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Genes, Reporter
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genetics
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Genistein
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chemistry
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isolation & purification
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HeLa Cells
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Humans
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Luciferases
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genetics
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metabolism
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Mice
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Models, Chemical
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Saccharomyces cerevisiae Proteins
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biosynthesis
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genetics
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Transcription Factors
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biosynthesis
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genetics
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Transfection
6.Statin reduced triglyceride level via activating peroxisome proliferator activated receptor α and upregulating apolipoprotein A5 in hypertriglyceridemic rats.
Xian-sheng HUANG ; Shui-ping ZHAO ; Lin BAI ; Qian ZHANG ; Min HU ; Wang ZHAO
Chinese Journal of Cardiology 2010;38(9):809-813
OBJECTIVEto explore the potential role of apolipoprotein A5 (apoA5) on the hypertriglyceridemia (HTG)-lowering effects of statin.
METHODStwenty-four Sprague-Dawley rats were randomized into 3 groups: (1) control group (n = 8), with no special treatment; (2) HTG group (n = 8), treated with 10% fructose water for 6 weeks; (3) statin group (n = 8), treated with 10% fructose water for 2 weeks and cotreated with atorvastatin 10 mg×kg(-1)×d(-1) for another 4 weeks. Body weight, fasting plasma lipids and the hepatic expressions of apoA5 and peroxisome proliferator activated receptor (PPAR)α were determined. In separate in vitro experiments, we tested the effects of atorvastatin on TG and the expressions of apoA5 and PPARα in HepG2 cells.
RESULTS(1) at 6 weeks, plasma TG was higher in rats in HTG group than in controls, which was significantly reduced in statin group (both P < 0.05). (2) Rat hepatic apoA5 expression in HTG group was significantly lower than in control group and was significantly higher in statin group than in HTG group (both P < 0.05). (3) Similarly, rat PPARα mRNA expression in HTG group was lower than in control group and was higher in statin group than in HTG group (both P < 0.05). (4) Statin significantly upregulated the expressions of apoA5 and PPARα and decreased TG in HepG2 cells. The above effects induced by statin was blocked in the presence of PPARα inhibitor.
CONCLUSIONSupregulation of apoA5 expression contributes to TG lowering effect of statin via PPARα signaling pathway.
Animals ; Apolipoprotein A-V ; Apolipoproteins ; blood ; Atorvastatin Calcium ; Down-Regulation ; Hep G2 Cells ; Heptanoic Acids ; pharmacology ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; pharmacology ; Hypertriglyceridemia ; metabolism ; Male ; PPAR alpha ; metabolism ; Pyrroles ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Triglycerides ; blood ; Up-Regulation
7.Transcatheter interventional therapy of congenital heart disease: the results of Chinese TIT registry.
Jun-jie LI ; Zhi-wei ZHANG ; Ming-yang QIAN ; Yu-fen LI ; Shu-shui WANG ; null
Chinese Journal of Cardiology 2012;40(4):283-288
OBJECTIVETo report the results of transcatheter interventional therapy (TIT) of congenital heart disease (CHD) register from 23 medical centers in China.
METHODIn this retrospective multicenter registry study, clinical data from 5808 patients who underwent TIT between January 2008 to December 2010 in 23 Chinese medical centers in 14 cities were analyzed.
RESULTSProcedure was successful in 5720 cases (98.5%), success rate was 99.5% for PDA, 98.8% for ASD, 97.4% for VSD and 98.5% for pulmonary stenosis (PS). Multivariate regression analysis showed that PDA size and procedure time, age and procedure time, distance from VSD to AV were significantly associated with the procedure success rate of PDA, ASD and VSD closure, respectively. Early complications occurred in 306 cases (5.3%), 36 cases (0.6%) experienced major complications including device embolization in 7 cases, serious aorta regurgitation in 5 cases, serious tricuspid regurgitation in 4 cases, tricuspid stenosis in 2 cases, heart block (HB) in 13 cases (2 in ASD and 11 in VSD), cardiac tamponade in 2 cases (1 ASD and 1 PS) and hemolysis in 3 cases. Procedure time and PDA size, ASD size, device size, age and PS degree were risk factors related to the occurrence of the early complications for PDA, ASD and VSD closure and PBPV respectively. The median follow-up time was 15 months (range 1-36 months). The complete closure rate during follow up was 100% for ASD, PDA and VSD and the pressure gradient in PS decreased to normal range in all PS patients. Late complications occurred in 15 cases (0.2%), of which 3 cases needed surgery intervention and permanent pacemaker was implanted in 1 patient. There was no death during procedure and at follow-up period.
CONCLUSIONSTIT of CHD offers encouraging results in China. Follow up is warranted to monitor the occurrence of serious complications, especially late complications.
Adolescent ; Adult ; Cardiac Catheterization ; Child ; Child, Preschool ; China ; epidemiology ; Ductus Arteriosus, Patent ; surgery ; Female ; Heart Defects, Congenital ; epidemiology ; surgery ; Heart Septal Defects, Atrial ; surgery ; Heart Septal Defects, Ventricular ; surgery ; Humans ; Infant ; Male ; Middle Aged ; Pulmonary Valve Stenosis ; surgery ; Registries ; Retrospective Studies ; Young Adult
8.Effects of trichostatin A on the expressions of inflammatory cytokines and toll-like receptor 4 and the acetylation of nuclear factor-κB induced by lipopolysaccharide in macrophage.
Xiao-Lan HU ; Xiao ZHANG ; Qian LI ; Shui-Feng QIU ; Ru-Huan MEI
Acta Physiologica Sinica 2012;64(6):651-656
The present study aims to explore the possible mechanisms that trichostatin A (TSA), a histone deacetylases inhibitor (HDACi), affects the inflammatory signaling pathways of lipopolysaccharide/toll-like receptor 4/nuclear factor-κB (LPS/TLR4/NF-κB). Murine macrophage cell line RAW264.7 cells were employed. MTT assay was used to assess cell viability. The contents of TNF-α, IL-1β and IL-6 in culture supernatant were assayed by enzyme-linked immunosorbent assay (ELISA). TLR4 expression and NF-κB/p65 (Lys310) acetylation were examined by Western blotting. DNA binding activity of NF-κB/p65 was detected by using TransAM(TM) NF-κB/p65 activity assay kit. The results showed that, compared with control group, which was treated by DMSO, the cells treated with TSA (20, 40, 80 ng/mL) showed decreased percentages of cell survival (P < 0.05). The contents of TNF-α, IL-1β and IL-6 in culture supernatant were all increased by LPS (100 ng/mL), whereas reduced by 40 ng/mL TSA pretreatment (P < 0.05). TSA pretreatment inhibited LPS-induced up-regulation of TLR4 protein expression. Acetylation of NF-κB/p65(Lys310), which was already increased by LPS, was further enhanced by TSA (P < 0.05). On the contrary, LPS-increased DNA binding activity of NF-κB/p65 was decreased by pretreatment with TSA (P < 0.05). The results suggest that TSA-induced anti-inflammation may be attributed to decreases in the expression of TLR4 and DNA binding activity of NF-κB/p65.
Acetylation
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Animals
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Cell Line
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Hydroxamic Acids
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pharmacology
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Inflammation
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metabolism
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Interleukin-1beta
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metabolism
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Interleukin-6
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metabolism
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Lipopolysaccharides
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Macrophages
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drug effects
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metabolism
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Mice
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Signal Transduction
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Toll-Like Receptor 4
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metabolism
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Transcription Factor RelA
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metabolism
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Tumor Necrosis Factor-alpha
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metabolism
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Up-Regulation
9.Sequence analysis of a novel human leukocyte antigen allele B*5827.
Chao-xia LU ; Na ZHU ; Qian ZHANG ; Hong HUANG ; Bing-shen KE ; Huai-shui HOU ; Bai-jun SHEN
Chinese Journal of Medical Genetics 2011;28(1):88-91
OBJECTIVETo investigate the molecular basis for a novel human leukocyte antigen (HLA) allele B*5827.
METHODSDNA from the proband was analyzed by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified product was sequenced bidirectionally.
RESULTSAbnormal HLA-B locus was observed and its nucleotide sequence was different from the known HLA-B allele sequences, with highest homology to HLA-B*5820 allele. It differs from HLA-B*5820 by 8 nucleotide substitutions in exon 3, i.e., nt 290 (G > C), nt 346 (T > A), nt 390 (A > C), nt 404 (G > C), nt 413 (C > G), nt 471 (A > G), nt 486 (A > G) and nt 487 (C > A), resulting in an amino acid change from ser > arg at nt 97, phe >tyr at nt 115, ser > arg at nt 130, thr > ala at nt 157 and thr > glu at nt 162. Nucleotide differences of nt 404 (G > C) and nt 413( C > G) did not change amino acid.
CONCLUSIONThe sequences of the novel allele have been submitted to GenBank (access No.GU071234). A novel HLA class I allele B*5827 has been officially assigned by the WHO HLA Nomenclature Committee in Jan. 2010.
Alleles ; Base Sequence ; Cloning, Molecular ; Genotype ; HLA-B Antigens ; chemistry ; genetics ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Analysis, DNA
10.Increased serum apolipoprotein A5 in patients with acute coronary syndrome.
Xian-sheng HUANG ; Shui-ping ZHAO ; Qian ZHANG ; Lin BAI ; Min HU ; Wang ZHAO
Chinese Journal of Cardiology 2009;37(10):896-899
OBJECTIVETo explore the relationship between serum apolipoprotein A5 (ApoA5) and lipid profile or high sensitive C-reactive protein (hs-CRP) in patients with acute coronary syndrome (ACS).
METHODSSerum apoA5 and hs-CRP levels were measured by ELISA and immunoturbidimetry in control subjects (n = 232), patients with stable angina (SA, n = 127), unstable angina (UA, n = 116) and acute myocardial infarction (AMI, n = 112). Triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were also measured.
RESULTSCompared with controls [(108.7 +/- 23.2) microg/L] and SA patients [(78.3 +/- 20.2) microg/L], serum ApoA5 level was significantly increased in UA [(340.6 +/- 63.5) microg/L] and AMI patients [(373.2 +/- 73.8) microg/L] (all P < 0.05). ApoA5 was positively correlated with TG (r = 0.63 and 0.67, respectively, all P < 0.05) and hs-CRP (r = 0.57 and 0.55, respectively, all P < 0.05) in UA and AMI patients but there were no significant correlations between ApoA5 and TC, HDL-C and LDL-C in ACS patients (all P > 0.05).
CONCLUSIONIncreased serum apoA5 level and the positive correlation between ApoA5 and serum TG and hs-CRP in ACS patients might reflect increased inflammation responses in ACS patients.
Acute Coronary Syndrome ; blood ; Aged ; Apolipoprotein A-V ; Apolipoproteins A ; blood ; C-Reactive Protein ; metabolism ; Female ; Humans ; Male ; Middle Aged ; Triglycerides ; blood