1.Diagnostic Value of Serum GP73 Combined with Dickkopf-1 and AFP in Hepatocellular Carcinoma
Fan YAO ; Liang LU ; Liao ZHANG ; Kejia ZHENG ; Shui JIN
Progress in Modern Biomedicine 2017;17(26):5153-5156
Objective:To investigate the diagnostic value of serum GP73 combined with Dickkopf-1 and AFP in hepatocellular carcinoma.Methods:117 cases of hepatocellular carcinoma in our hospital were collected as hepatocellular carcinoma group,80 patients with hepatitis B virus as the hepatitis group,and randomly selected 80 healthy subjects as the control group from September 2014 to September 2016.The serums GP73,Dickkopf-1,AFP level of every group were detected by the enzyme-linked immunosorbent assay (ELISA),and the relationship between the GP73,Dickkopf-1,AFP between clinicopathological features were analyzed,the diagnostic value of GP73,Dickkopf-1 and AFP in hepatocellular carcinoma were analyzed.Results:The serums GP73,Dickkopf-1,AFP level of hepatocellular carcinoma group were higher than those of hepatitis group,control group,and the serum AFP level of hepatitis group was higher than that of control group,the difference was statistically significant (P<0.05).The GP73,Dickkopf-1,AFP in patients with classification B~C were higher than those in classification A among the Child Pugh classification,the difference was statistically significant (P<0.05).No statistical significance on difference of GP73,Dickkopf-1,AFP between different differentiation,tumor size,number of tumors was found (P>0.05).The sensitivity,specificity,positice predictive value,negative predictive value,and accuracy of GP73+Dickkopf-1+AFP in the diagnosis of hepatocellular carcinoma were higher than those of GP73,Dickkopf-1,AFP respectively,the difference was statistically significant (P<0.05).Conclusion:Serums GP73,Dickkopf-1,AFP in patients with hepatocellular carcinoma have high expression levels,combined with the three indicators can obviously improve the diagnostic value of hepatocellular carcinoma,which has clinically important reference value.
2.Selection of the internal fixation for various types of intertrochanteric fracture in aged patients and prevention for complication.
Xin GE ; Jian-Ming ZHANG ; Wei-Cheng LU ; Shui-Qiang QIU
China Journal of Orthopaedics and Traumatology 2009;22(5):385-386
OBJECTIVETo investigate the results and indication of differently internal fixation for intertrochanteric fracture and prevent for complication.
METHODSOne hundred and two patients of intertrochanteric fracture of femur from January 1997 to December 2007 were reviewed and analysed. There were 46 males and 56 females, with an average age of 76.5 years ranging from 60 to 93 years. There were 34 cases of stability fractures and 68 of instability fractures. The methods of internal fixation included dynamic hip screw in 82 cases, proximal interlocking nail in 5, multi-screw fixation in 9, dynamic codylar screw in 6.
RESULTSThese patients were followed up for 6 to 72 months (36 months in average). The time of fracture healing was from 8 to 12 weeks. According to evaluation criterion of effect, the results were excellent in 72 cases, good in 20, fair in 10. After operation, there were 3 cases of coax vara, 2 cases of lag screws cutting out, 1 case of breakage of screw, 2 cases of avascular necrosis of the femoral head, 1 case of postoperative infection, 1 case of delayed union.
CONCLUSIONSuitable implant selection according the type of fracture, health condition is an important factor to reduce the incidence of complication for unstable fracture in aged patients. DHS is a main treatment of intertrochanteric fracture. PFN is recommended for serious osteoporosis or unstable intertrochanteric fracture. Compression screws is a choice for the fixation of type I and type II fracture of bad health condition.
Aged ; Aged, 80 and over ; Bone Screws ; Female ; Fracture Fixation, Internal ; methods ; Fracture Fixation, Intramedullary ; methods ; Fracture Healing ; physiology ; Hip Fractures ; complications ; surgery ; Humans ; Joint Instability ; physiopathology ; Male ; Middle Aged ; Postoperative Complications ; prevention & control
3.Dynamic changes in plasma D-dimer level and its prognostic value in children with severe hand-foot- mouth disease.
Chinese Journal of Contemporary Pediatrics 2013;15(12):1119-1122
OBJECTIVETo study the dynamic changes in plasma D-dimer and its prognostic value in children with severe hand-foot-mouth disease (HFMD).
METHODSA total of 95 children who suffered from HFMD between May 2010 and September 2012, including 65 cases of severe HFMD (observation group) and 30 cases of non-severe HFMD (control group), were enrolled in the study. Plasma D-dimer levels of the observation group on days 1, 2, 3, 4 and 5 were compared with plasma D-dimer levels of the control group on day 1 after admission. In the observation group, plasma D-dimer levels on days 1, 2, 3, 4 and 5 were compared. The prognostic value of plasma D-dimer was analyzed using the receiver operating characteristic (ROC) curve.
RESULTSOf the 65 cases in the observation group, 15 died, and 50 survived. All the 30 cases in the control group survived. Plasma D-dimer levels in the observation group on days 1, 2, 3, 4 and 5 were significantly higher than in the control group on day 1 after admission (P<0.05). In the observation group, there were significant differences between plasma D-dimer levels on days 1, 2, 3, 4 and 5 (P<0.01), and plasma D-dimer level was the highest on day 1 after admission and second highest on the next day. Of the patients in the observation group, those who died had significantly higher plasma D-dimer levels on day 1 after admission than those who survived (P<0.05). In the observation group, plasma D-dimer levels on day 1 after onset had an area under the ROC curve of 0.877 (95% confidence interval: 0.785-0.969) and an optimal cut-off value of 582.10 μg/L (80% sensitivity and 78% specificity) for predicting mortality.
CONCLUSIONSChildren with severe HFMD have significantly increased plasma D-dimer levels, and the severer the condition, the higher the value. Plasma D-dimer levels can be used as one of the important indices for assessing the severity and prognosis of severe HFMD.
Child ; Child, Preschool ; Female ; Fibrin Fibrinogen Degradation Products ; analysis ; Hand, Foot and Mouth Disease ; blood ; mortality ; Humans ; Infant ; Male ; Prognosis
4.Study of Bilingual Teaching for Medical Microbiology
Shui-Xiang XIE ; Xiao-Li WANG ; Wen-Ping ZHANG ; Lian-Lan MA ; You-Tian ZHONG ; Gao-Lu CAO ; Zhen HUANG ;
Microbiology 1992;0(01):-
Bilingual teaching is adapted to the development of higher education in china.Based on actual fact of college,teaching mode,evaluation and effect of bilingual teaching on medical microbiology were studied,which started with necessity of bilingual teaching to use original edition teaching material in English. The result would provide some gist to choice the suitable pattern of bilingual teaching for other subject of our college.
5.The application of network scale-up method on female sex workers and clients size estimation in Taizhou city.
Xi-ping HUAN ; Shui-lian BAO ; Hai-tao YANG ; Jin-shui XU ; Tao QIU ; Xiang ZHANG ; Long PAN ; Zhong-kui ZHU ; Wei GUO ; Lu WANG
Chinese Journal of Preventive Medicine 2013;47(3):233-237
OBJECTIVETo estimate the size of female sex workers and clients in Taizhou city.
METHODSA household survey using network scale-up method (NSUM) was conducted among the 3000 community residents in Taizhou city from August to October in 2011, which aimed to estimate the social network size (c value) of Taizhou residents, and the c value was adjusted by demographic characteristics, back estimation and outlier elimination. Using the adjusted c value, the number of acquaintance of female sex workers or clients and the respect level toward female sex workers or clients were used to estimate the size of female sex workers and clients.
RESULTSA total of 2783 valid questionnaires were collected, among which 1380 (49.6%) were collected from Taixing city, 1403 (50.4%) were collected from Jingjiang city. 1334 respondents were male (47.9%) and 1449 (47.9%) respondents were female. The mean age was (39.4 ± 10.7) years. The average personal social network size using original data for Taizhou residents was 525, which differed from place, sex, age, educational level and marriage status. Using the remaining known populations through back estimation, the social network size was 419 and became 424 after the elimination of outliers. The estimated population size for female sex worker was 6370 (95%CI: 5886 - 6853), which accounted for 0.52% (6370/1 229 980) of the total number of female aged from 15 to 49. The estimated population size for clients was 15 202 (95%CI: 14 560 - 15 847), which accounted for 1.28% (15 202/1 190 340) of the total number of males aged from 15 to 49 and the ration of clients to female sex worker was 2.39:1.
CONCLUSIONNSUM is an easy and quick way to estimate the size of female sex workers or clients, but the estimated sizes are subject to bias and error due to estimate effect and sample representativeness.
Adolescent ; Adult ; China ; Female ; Humans ; Male ; Middle Aged ; Sex Work ; statistics & numerical data ; Sex Workers ; statistics & numerical data ; Surveys and Questionnaires ; Young Adult
6.Development and application of a mammlian one hybrid-based high-throughput screening model for Eralpha modulator.
Qian ZHANG ; Xiaoxi SHUI ; Yuling FAN ; Weili HAO ; Zhihui ZHENG ; Xinhua LU ; Baohua ZHAO ; Hua ZHANG ; Jiangong HE
Chinese Journal of Biotechnology 2009;25(7):1088-1094
Estrogen Receptor (ERalpha) is a member of superfamily of ligand-activated transcription factors which play critical roles in many biological processes. To screen novel modulators of ERalpha for drug development and biological function research, we developed a mammalian one-hybrid-based high-throughput screening model for ERalpha modulator. We cloned the ERalpha LBD gene from the total mRNA of fat tissue by RT-PCR and fused it with the GAL4 DNA binding domain of pBIND-GAL4 plasmid to construct a chimara expression plasmid pBIND-GAL4-Eralpha(LBD). The L02 cells was cotransfected with pBIND-GAL4-ERalpha(LBD) and a GAL4-responsive luciferase reporter plasmid pGL3-GAL4, and following treatment with test compounds for 24 h, the activities of luciferase were detected to evaluate the transactivities of ERalpha modulators. After manner optimizations of transfection conditions, Estradiol, an agonist control, induced the expression of luciferase in a dose-dependent with EC50 of 0.17 micromol/L, the maximum folds of induction was about 28.1. Tamoxifen, an antagonist control, efficiently suppressed the estradiol-mediated luciferase induction with EC50 of 0.10 micromol/L. Using this screening model, we discovered four ERalpha agonists from 2000 natural and synthetic compounds.
3T3-L1 Cells
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Animals
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Chimera
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metabolism
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DNA-Binding Proteins
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biosynthesis
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genetics
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Estrogen Receptor Modulators
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chemistry
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isolation & purification
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Estrogen Receptor alpha
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agonists
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Genes, Reporter
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genetics
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Genistein
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chemistry
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isolation & purification
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HeLa Cells
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Humans
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Luciferases
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genetics
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metabolism
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Mice
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Models, Chemical
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Saccharomyces cerevisiae Proteins
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biosynthesis
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genetics
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Transcription Factors
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biosynthesis
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genetics
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Transfection
7.Anatomic study of internal fixation of acetabular anterior column plate technique.
Xian-quan WANG ; Wei ZHANG ; Shui SUN ; Jin-lu ZHANG ; Jian WANG ; Wei LI
Chinese Journal of Surgery 2006;44(24):1700-1703
OBJECTIVETo study the best entry points, direction and length of screw in acetabular anterior column plate technique, and to prevent the serious complications of screw penetrating the joint surface.
METHODSTwenty male cadaveric adult semi-pelvic specimen were taken, and the distance from anterior acetabular margin, posterior acetabular margin to anterior inferior iliac spine, iliopubic eminence and pubic tubercle were measured respectively. Determine and make serial cross-sections of the acetabular anterior column, measure the safe angle of screw entry on all entry points of each cross-section, and put all data into software SPSS 10.0 for statistics process.
RESULTSThe distance from anterior acetabular margin to anterior inferior iliac spine, iliopubic eminence and pubic tubercle was (25.4 +/- 1.4) mm, (11.8 +/- 0.7) mm and (37.4 +/- 1.5) mm respectively, the distance from posterior acetabular margin to anterior inferior iliac spine, iliopubic eminence was (15.5 +/- 0.9) mm and (29.1 +/- 1.6) mm respectively. On each cross-section, the maximum of the safe entry angle of inclination in 0.5 cm, 1.0 cm and 1.5 cm entry point lateral to the linea terminalis of pelvis was (8.2 +/- 2.2) degrees , (14.9 +/- 3.4) degrees and (26.1 +/- 4.5) degrees respectively.
CONCLUSIONSWhen plate for internal fixation on acetabular region of anterior column is used, there are three ways to avoid screw penetrating the joint surface. The first way is using short screw in any direction; the second way is using long screw close to the linea terminalis of pelvis, the direction of the screw is parallel to the quadrilateral plate; the third way is using different entry angle and length according to different entry point.
Acetabulum ; anatomy & histology ; surgery ; Adult ; Bone Plates ; Bone Screws ; Cadaver ; Fracture Fixation, Internal ; instrumentation ; methods ; Humans ; Male ; Models, Anatomic
8.Sequence analysis of a novel human leukocyte antigen allele B*5827.
Chao-xia LU ; Na ZHU ; Qian ZHANG ; Hong HUANG ; Bing-shen KE ; Huai-shui HOU ; Bai-jun SHEN
Chinese Journal of Medical Genetics 2011;28(1):88-91
OBJECTIVETo investigate the molecular basis for a novel human leukocyte antigen (HLA) allele B*5827.
METHODSDNA from the proband was analyzed by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified product was sequenced bidirectionally.
RESULTSAbnormal HLA-B locus was observed and its nucleotide sequence was different from the known HLA-B allele sequences, with highest homology to HLA-B*5820 allele. It differs from HLA-B*5820 by 8 nucleotide substitutions in exon 3, i.e., nt 290 (G > C), nt 346 (T > A), nt 390 (A > C), nt 404 (G > C), nt 413 (C > G), nt 471 (A > G), nt 486 (A > G) and nt 487 (C > A), resulting in an amino acid change from ser > arg at nt 97, phe >tyr at nt 115, ser > arg at nt 130, thr > ala at nt 157 and thr > glu at nt 162. Nucleotide differences of nt 404 (G > C) and nt 413( C > G) did not change amino acid.
CONCLUSIONThe sequences of the novel allele have been submitted to GenBank (access No.GU071234). A novel HLA class I allele B*5827 has been officially assigned by the WHO HLA Nomenclature Committee in Jan. 2010.
Alleles ; Base Sequence ; Cloning, Molecular ; Genotype ; HLA-B Antigens ; chemistry ; genetics ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Analysis, DNA
9.Influence of propofol and isoflurane on cytokines response to cancer surgery during perioperative period.
Cong YU ; Yu-lin LUO ; Shui-sheng XIAO ; Qing ZHANG ; Si-lu CHEN
West China Journal of Stomatology 2007;25(6):554-556
OBJECTIVETo compare the influence of propofol and isoflurane on pro-inflammatory and anti-inflammatory cytokine response to perioperative period of tongue cancer surgery.
METHODSTwenty-four adult patients undergone the operation of tongue cancer were assigned to two groups randomly, propofol group (Group P) and isoflurane group (Group I). In group P, anesthesia was induced with fentanyl 2-3 microg/kg, propofol 2 mg/kg, atracurium 0.6 mg/kg and maintained with propofol 5-8 mg x kg(-1) x h(-1) and inhalation of 50% nirous oxide (N2O:O2=50%:50%). In group I, anesthesia was induced with 3%-4% isoflurane, fentanyl 2-3 microg/kg, diazepam 0.06-0.1 mg/kg, atracurium 0.6 mg/kg and maintained with inhalation of 50% N2O and isoflurane (ended-tidal isoflurane was maintained at 0.6%), in two groups atracurium was given intermittently. Blood samples were taken from peripheral vein before anesthesia (TO), at the end of operation (T1), 24 h (T2) and 48 h (T3) after operation for determination of serum IL-6 and IL-10 concentrations. The mean arterial pressure (MAP) and body temperature in two groups were recorded.
RESULTSIL-6 and IL-10 levels increased significantly in two groups at T1, T2 and T3 compared with T0 (P < 0.01). The increasing trend of IL-6 and IL-10 levels were similar in both groups, whereas the level of IL-6 at T1 in propofol group was lower than that of isoflurane group significantly (P < 0.01), however the level of IL-10 was much higher in propofol group than that of isoflurane group at T1 and T2 (P < 0.05).
CONCLUSIONThe influence of total intravenous anesthesia of propofol on post-operation inflammatory response is much gentler than isoflurane.
Adult ; Anesthesia, Intravenous ; Cytokines ; Female ; Humans ; Interleukin-10 ; Interleukin-6 ; Isoflurane ; Male ; Middle Aged ; Neoplasms ; Perioperative Period ; Postoperative Period ; Propofol
10.Fungal granuloma of mediastinal lymph nodes in an immunocompetent host.
Xue-yuan CHEN ; Hui-ping LI ; Rong-xuan ZHANG ; Shui-hua LU
Chinese Medical Journal 2011;124(15):2396-2399
This is a case report of mediastinal fungal granuloma in an immunocompetent host. The definite diagnosis was made by pathological biopsy via video-assisted thoracoscopy and silver methenamine staining showed aspergillus hyphae and spores in the epithelioid granuloma. In conclusion, opportunistic pathogenic fungi can cause granulomatous inflammation in mediastinal lymph nodes in an immunocompetent host, as it can do in an immunocompromised host. More attention should be paid on tissue biopsy and pathological examination to ensure a correct diagnosis for these kinds of cases.
Adolescent
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Fungi
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immunology
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pathogenicity
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Granuloma
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diagnostic imaging
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immunology
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microbiology
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Humans
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Immunocompromised Host
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immunology
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Lymph Nodes
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diagnostic imaging
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immunology
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microbiology
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Male
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Mediastinum
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diagnostic imaging
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Radiography