Acupuncture Points ; Acupuncture Therapy ; Adult ; Esophageal Achalasia ; therapy ; Humans ; Male

Objective To observe the toxic effects of2-methyl-4-chlorophenoxyacetic acid(MCPA)in male mice.Meth-ods The Kunming male mice were divided into4groups,10male mice per group.The control group were purfused into stom-ach by distilled water,the other three groups were perfused into stomach by20,100and200mg /kg MCPA,once a day,6times per week,continuously for17days.The body weight ,T 4 ,TSH,cholesterol,the ratio of spleen and testis to body weight and the specific activity of LDH,SDH in the testis were analyzed.Results The body weight of mice in MCPA-exposure groups in-creased more slowly than that in the control group.The contents of TSH in serum and ratio of spleen to body weight in200mg/kg group were significantly lower than those in control group.The contents of cholesterol in serum increased with the increases of MCPA-exposure doses.The contents of T 4 in serum were significantly higher in20mg /kg group and lower in200mg /kg group compared with that in control group.The ratio of the testis to body weight reduced with increase of the MCPA-exposure doses,and the specific activity of LDH in the testis of MCPA-exposure groups increased significantly,but the specific activity of SDH in the testis of MCPA-exposure groups revealed no significant variations compared with that of control group.Conclusion MC-PA inhibited the growth of male mice,resulted in endocrine disorder,the atrophy of spleen,and presented the toxicity of repro-ductive system.

Objective To study the effect of nitrobenzene(NB) on the activity of enzymes and the content of zinc in the testis tissue of mice. Methods 40 male mice were divided into 4 groups, 10 in each, 3 groups were treated with NB at the doses of 2, 20, 200 mg/kg by gavage, the control group was treated with vegetable oil in the same volume, once a day, for 21 consecutive days. The activity of G-6-PD, LDH and the content of Zn2+ in testis cells were determined. Results The index of epididymis and testis in the groups of 200 mg/kg were significantly lower compared with the control and 2 mg/kg group (P0.05). The activity of G-6-PD, LDH and the content of Zn2+ in all the treated groups were lower than those in the control group (P

The study assessed the early functional outcomes with cemented titanium implants of radius in the treatment of comminuted fractures of radial heads. The functional outcomes of arthroplasty with cemented titanium implants of radius in the treatment of radial head fractures (Mason Type III: 6; Mason Type IV: 4) in 10 consecutive patients (mean age, 38 years) were evaluated over a mean time of 23.7 months (18-31 months). The patients were assessed on the basis of physical examination, functional rating (Mayo) and radiographic findings. The parameters evaluated included motion, stability, pain, and grip strength. Five patients were considered to have excellent results, 4 patients had good results and 1 patient had fairly good results. There were no cases of infection, prosthetic failure, heterotopic ossification or dislocation. When medial collateral ligament was injured, radial head became the main stabilizing structure of the elbow. Titanium radial head implant may provide the stability similar to that of native radial head. We believe that titanium radial head implants may be indicated for the Mason Type III and Mason Type IV radial head fractures.
Elbow Joint/surgery ; Fracture Fixation/*methods ; Fractures, Comminuted/*surgery ; Prostheses and Implants ; Radius Fractures/*surgery ; Titanium ; Treatment Outcome

Objective To explore the influence of self-efficacy training on the memory and executive function of patients with ischemic stroke in the recovery stage.Methods A total of 80 cases were randomly divided into a self-efficacy training group and a control group,with 40 cases in each.The control group was given routine cognitive therapy,while the self-efficacy group was given cognitive interventions plus efficacy training.Before training and after training for 3 months,self-efficacy levels were examined using the general self-efficacy scale (GSES),memory and executive function were measured using the Behavioral Assessment of Dysexecutive Syndrome (BADS) and the Wechsler Memory Scale (WMS).Anxiety and depression were evaluated using the Self-rating Depression Scale (SDS) and Self-Rating Anxiety Scale (SAS).Results Inter-group comparison showed no significant differences in terms of GSES or BADS scores before treatment between the control and self-efficacy groups.After training,the total GSES and BADS scores in both groups were significantly higher than before.Both the average GSES and BADS scores in the self-efficacy group were significantly higher than in the control group.There were similarly no significant differences in WMS scores before treatment,but after training the WMS results were significantly better in the self-efficacy training group than in the control group.After training,the SDS and SAS scores had improved to a significantly greater extent in the self-efficacy group than in the control group.Conclusion Self-efficacy training can facilitate the recovery of memory and promote executive function in patients with ischemic stroke.

Objective To study the protective effect of ginger extract on RBC of X-ray irradiated mice. Methods Mice were given extract of ginger with three doses, namely 9.3 ml/kg bw, 4.7 ml/kg bw and 2.3 ml/kg bw before X-ray irradiation(dose was 2.0 Gy, for two times, the interval was 24 h), 48 hours after the last irradiation, the blood samples were collected and RBC, HCB, HCT, MCV, MCHC, MCH, RDW were examined. Results Compared with the exposed control, RBC, HCB, HCT increased and MCH, RDW decreased significantly in ginger treated groups. Conclusion The ginger extract has a certain effect to prevent RBC from damaging induced by X-ray irradiation in mice.

0. 05). This paper surggests that the activity of Na+-K+ ATPase on erythrocyte membrane may be a indicator in the diagnosis of stroke and human senility. The mechanism of changes of Na+-K+ATPase was also discussed in this paper.

To determine the effect of straight-leg-raising (SLR) movement on epidural fibrosis after laminectomy, 40 adult New Zealand rabbits were selected as laminectomy models in the study. They were divided into 2 groups: a SLR group (group S) and a control group (group C) randomly, with each group having 20 animals. All rabbits were subjected to total laminectomy in the site of S1. Every 5 rabbits in each group selected randomly were killed at the 1st, 2nd, 4th, and 8th week after the surgery. Segments of spines from L7 to S2 were removed en bloc. After gross evaluation, specimens were sliced up. The slices were stained by HE and Masson's trichrome methods respectively for histological examination. Our results showed that formation process of scar in group S was retarded as compared with that of group C at the time of the 2nd-week, but there was no statistical difference between groups in the adhesion degree (P> or =0.05). At the 4th and 8th week, the epidural fibrosis of group S was more serious than that of group C. Since the 2nd-week, the area of scar in group S was larger than that of group C. The number of fibroblasts and inflammatory cells in group S were larger than those of group C at early stage. But in later stage, there was no statistical significance between the two groups. It is concluded that SLR movement after laminectomy may promote the formation of epidural fibrosis and retard the maturity of scar. SLR movement can also aggravate scar adhesion.

AIM:To investigate the time course of nuclear factor-κB (NF-κB) and the effects of 3-aminobenzamide (3-AB) on the expressions of NF-κB,interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in hippocampus after seizures. METHODS:Epilepsy were induced by kainic acid through cerebral ventricular injection. Western blotting was used to detect NF-κB p65 expression in nucleus at various experiment groups. Moreover,mRNA and protein expressions of IL-1β and COX-2 in different experiment groups were determined by RT-PCR and Western blotting analysis. RESULTS:NF-κB p65 immunoreactivity began to increase in the nuclear fraction at 2 h (P<0.05),kept rising at 12 h (P<0.05) and returned to control level at 24 h after epilepsy seizures. Furthermore,3-AB sharply decreased the accumulation of NF-κB p65 in nucleus (P<0.05). In addition,3-AB significantly decreased the mRNA and protein expressions of IL-1β and COX-2 which obviously increased in hippocampus at 6 h after epilepsy seizures (P<0.05). CONCLUSION:Seizures triggers NF-κB nucleus translocation and promotes the expressions of IL-1β and COX-2 in hippocampus. In addition,poly (adenosine diphosphate-ribose) polymerase inhibition by 3-AB suppresses NF-κB associated inflammatory pathway in epileptic rat hippocampus.

Objective To investigate the expressions of serum cystatin C CysC),retinolbinding protein (RBP),serum and urinary β2-microglobulin (β2-MG) and their effects on predicting renal damage in primary hypertension in elderly patients.Methods A total of 149 patients were divided into 3 groups of hypertension grade 1 (n =50),hypertension grade 2 (n =50) and hypertension grade 3 group (n=49).92 healthy controls who took physical examination were selected as control group.The levels of serum CysC,RBP,serum and uric β2-MG,blood urea nitrogen,creatinine and other biochemical indexes were detected.The complications of hypertension were observed.The differences in renal function indexes between groups and their correlations were analyzed.Results There were 86 cases with hyperlipidemia (57.7％),71 cases with atherosclerosis (47.7％),59 cases with type 2 diabetes mellitus (39.6％),56 cases with coronary heart disease (37.6％),and 49 cases with cerebral vascular disease (32.9％) in 149 hypertensive patients.The levels of serum CysC,RBP,serum and uric β2-microglobulin were increased in hypertensive patients as compared with healthy controls (P＜0.05 or 0.01),and the extents of increments in the above indexes were positively correlated with blood pressure level [CysC:(1.2± 0.5) mg/L,(1.7±0.6) mg/L,(2.2±0.9) mg/L;RBP:(53.1±10.2) mg/L,(65.2±16.3)mg/L,(75.8±17.5) mg/L;serum β2-MG:(2.7±1.5) mg/L,(4.0±2.1) mg/L,(4.9±2.0) mg/L;uric β2-MG:(178.3±73.9) ng/L,(237.9±78.6) ng/L,(277.5±87.5) ng/L;respectively,P＜0.05or 0.01].Conclusions Elderly hypertensive patients are complicated with a variety of diseases.The levels of serum CysC,retinol-binding protein,serum and uric β2 microglobulin have significant diagnostic values on renal impairment caused by hypertension,and can be considered as sensitive factors for early renal impairment in essential hypertension.