Easily confusable seed of Ziziphus jujuba var. spinosa (Bunge ) Hu and Hovenia acerba Lindl., Allium tuberosum Rottl .ex spreng. and A. fistulosum; Sinapis alba L. and Brassica campestris L. were examined under scanning electron microscope. Results s howed that: 1. )irregular nests were present on the surface of semen Z. jujuba var. spinosa, while papillae appeared on the surface of senen H.acerba Lindl; 2. ) Cells of seed coat of semen A. tuberosum were irregularly shaped or polygonic with protruding cutin on its peridium, while that of Semen A. fistulcsum were polygonic with connecting fibers between cells; 3. ) cells of semen S.alba L. were similarily polygonic, with thick and protruding vertical perine,while that of semen B. campestris L. were also polygonic, but its vertical perine were thiner and less protruding, its peridium shrinked in side and was covered with cutinous nets veins.

Objective To investigate the clinical effect of biofeedback electrical stimulation in the treatment of female stress urinary incontinence(SUI) and discuss the clinical significance.Methods Daily therapy for 40 min through biofeedback electrical stimulation along with pelvic muscle exercises was performed in 36 cases of female SUI for 20 days as a course of treatment.The curative effect was evaluated as regular intervals.Results In the 36 cases,26 caces were cured,5 caces were improved and 5 caees were invalid.The total effective rate was 86.1％.In the follow-up visit,there was 1 case of relapse(2.8％ ) but with moderate symptoms.Conclusion Biofeedback electrical stimulation combined with pelvic muscle exercises,as the therapy for mild and moderate female SUI,was a safe,reliable,feasible,convenient and effective clinical method with high curative ratio.

Objective To investigate therapeutic effects of CT-guided percutaneous minimally invasive treatment by using Herbert screws for wrist scaphoid fracture . Methods From September 2010 to October 2012, 21 patients with scaphoid fracture were treated by internal fixation by using Herbert screws under the guidance of CT .The results of the procedure were assessed in terms of osseous union , pain extent , grip strength , and active motion of the wrist .Postoperative outcomes were evaluated by wrist standard scores based on patients’ perception of functions. Results The operation time was 25 -55 min (mean, 35.3 min).The intraoperative blood loss was 8-40 ml (mean, 18.6 ml).Fracture fragments were cured with bone union , with an average healing time of 10.4 weeks (range, 8-17 weeks).All the 21 cases were followed up for 8 -13 months (mean, 9.8 months).The VAS scores at the last follow-up was (1.2 ±0.8) points, which was significantly lower than that before the operation (5.3 ±1.4) points (t=11.785, P=0.000).The grip strength was (39.6 ±3.5) kg, which was significantly higher than that preoperatively [(19.4 ± 2.4) kg, t=-21.624, P =0.000].The Angle of wrist flexion and extension was 115.2°±7.4°, significantly higher than preoperative measure (89.8°±10.4°, t =-9.103, P =0.000).The wrist ulnar radial deflection angle was 53.7°±5.5°, significantly higher than preoperative one (39.4°±3.4°, t =-10.116, P=0.000).At the last follow-up assessment of wrist functions, excellent result was recorded in 18 cases, and good in 3 cases, with a good-or-excellent rate of 100%. Conclusion Treatment of scaphoid fracture with Herbert screws under the guidance of CT is a minimally invasive method , bearing advantages of little incision, simple operation performance , and good effects .

ObjectiveTo explore the application of the plastic principles in surgical treatment for diabetic foot ulcer and analyze the therapeutic efficacy.Methods51 cases of diabetic foot ulcer repaired with surgical treatment were analyzed.Results26 cases repaired with free skin graft,1 1 cases repaired with skin flap graft,3 cases repaired with amputation,the percentage of first stage healing rate was 91.4％.ConclusionApplication of the plastic principles in surgical treatment for diabetic foot ulcer,could improve the healing percentage and reduce the rate of amputation.

liver after i.v. administration. The distribution of osthole in blood and kidney were siginificantly higher( P

Objective To construct a short hairpin RNA (shRNA) targeting LDH-A, and evaluate the effects on growth, apoptosis and the expression of LDH-A in PANC-1 cells. Method Three shRNAs targeting LDH-A were combined to pGCsilencer vector, and transfected into PANC-1 cells. The expression of LDH-A after transfected by the three shRNAs in pancreatic cancer cell lines PANC-1 was detected by quantitative real time PCR. After tranfected by LDH shRNA-3 with the highest inhibited rate, cell growth was analyzed by MTT assay, apoptosis was detected by flow cytometry. The LDH-A expression was detected by reverse transcription polymerase chain reaction, LDH activity was observed with enzyme cytochemical method. Results The 2-AACt of LDH-A shRNA-3 was (0. 47 ± 0. 02), less than the untransfected pancreatic cancer cell(0. 71 ± 0. 01), the LDH-A shRNA-3 could inhibit the expression of the LDH-A most effectively. The growth of the pancreatic cancer cell was inhibited after 12 h transfected by LDH-A shRNA-3, all the absorbance value of transfected cell in 24 h,36 h,48 h,72 h decreased obviously compared to the untreated pancreatic cancer cell(P <0. 01). The apoptosis rate of the transfected cell increased to 61.74%. The inhibition of LDH-A expression in PANC-1 cells transfected by shRNA-3 was significantly and the activity of LDH reduced. Conclusion LDH-A shRNA inhibits the expression of LDH-A, the proliferation of cancer cells inducing the apoptosis of PANC-1 cells.

Objective To investigate the effects of oxamate on concentrations of calcium in pancreatic cancer cells.Methods Pancreatic cancer cell panc-1 was cultured in vitro with oxamate for 48 h,then stained with Fluo-3/AM,and the light density of cells for different concentration of oxamate under confocal laser microscopy was observed.Results Oxamate influenced the concentration of calcium in panc-1,and this effect corresponded to the concentration of oxamate.Conclusions Oxamate can induce the increase of the concentration of calcium of panc-1,and then influence the cellular pathophysiologic process.

Objective To investigate infection pathogens and drug resistance of patients in the department of geriatrics ,so as to guide the rational use of antibacterial agents .Methods Retrospectively analysed data on clinical specimens of 1 563 cases of elderly patients from 2014 to 2013 .Results A total of 591 non‐repeated strains was isolated and the positive rate was 37 .8% .Strains mostly were isolated from sputum specimens (299 strains ,accounted for 50 .6% ) ,followed by urine specimens(171 strains ,accoun‐ted for 28 .9% ) ,secretion specimens(75 strains ,accounted for 12 .7% ) and blood specimens(15 strains ,accounted for 2 .5% ) .The isolated pathogenic bacteria mainly were gram‐negative bacteria(72 .60% ) ,which mostly were Pseudomonas aeruginosa .Acineto‐bacter baumannii ,Escherichia coli and Staphylococcus aureus has relatively high resistance rates against most of antibacterial agent . A total of 54 strains of multi‐drug resistant bacteria were isolated ,which was accounted for 56 .3% .Conclusion The pathogenic bacteria in the elderly patients mainly are gram‐negative bacteria ,pathogens have high resistance rates to antimicrobial agents ,and the multi‐drug resistance is serious ,so it is necessary to use antibacterial agents rationally based on the results of drug susceptibility tests .

Objective] To study Professor Wang Kungen's therapeutic principles in treating chronic constipation by data mining therapy. [Method]First, establish Wang Kungen's medical records database; then do data standardization and data mining. [Result]There are totally 207 cases that meet the demand. The major patterns of syndrome of CC in Professor Wang Kungen's medical records are as follows: gastrointestinal heat syndrome, stagnation of liver qi and spleen deficiency, Yin deficiency syndrome, Qi deficiency. The major prescriptions are as follows: Liumo Decoction, Xiao Yao San, Pi Yue Pill, Buzhong Yiqi decoction. There is close correlation between prescriptions and patterns as follows: gastrointestinal heat syndrome and Liumo Decoction; stagnation of liver qi and spleen deficiency and Xiao Yao San; Yin deficiency syndrome and Pi Yue Pill; Qi deficiency and Buzhong Yiqi decoction. There is close correlation between herbs as follows: rhizoma atractylodis macrocephalae and fructus arctii; rhizoma atractylodis macrocephalae and radix paeoniae alba; fructus cannabis and elecampane; rhizoma atractylodis and fuling; peach kernel and cortex moutan; semen raphani and fructus aurantii.[Conclusion] The evidence type of chronic constipation is relatively purging heat and keeping body fluid, soothing the liver and stomach, tonifying spleen and moistening intestines, tonifying Qi.

OBJECTIVE:To explore the clinical efficacy and safety of Yunkang granule combined with dydrogesterone in the treatment of early threatened abortion. METHODS:128 patients with early threatened abortion were randomly divided into single drug group and combined medication group,64 cases in each group. Single drug group received Dydrogesterone tablet with first dose of 40 mg,then 10 mg,q 12 h until vaginal bleeding;combined medication group additionally received 8 g Yunkang granule, tid,until symptoms disappeared. Human chorionic gonadotropin (HCG),clinical symptoms remission time and disappearance time,pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcome,and the incidence of adverse reac-tions in 2 groups were compared. RESULTS:After treatment,HCG in 2 groups significantly increased,and combined medication group was higher than single drug group,the differences were statistically significant(P<0.05);clinical symptoms remission time and disappearance time in combined medication group were shorter than single drug group,the difference was statistically signifi-cant (P<0.05). There were no significant differences in incidence of pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcomes in 2 groups (P>0.05). The incidence of adverse reactions in combined medication group was 10.9%, which was significantly lower than single drug group (40.6%),the difference was statistically significant (P<0.05). CONCLU-SIONS:Both dydrogesterone alone and Yunkang granule combined with dydrogesterone show good efficacy in the treatment of ear-ly threatened abortion,but the remission time and disappearance time of clinical symptoms and adverse reactions in combined medi-cation group are less than that in single drug group.