1.Efficacy of fixation with knee arthroscopy as adjuvant therapy for lateral tibial plateau fracture
Fang YE ; Shuhua LAN ; Quanzhou WU
China Journal of Endoscopy 2016;22(8):25-28
Objective To study the efficacy of fixation with knee arthroscopy as adjuvant therapy for lateral tibial plateau fracture. Methods 72 cases with lateral tibial plateau fracture of our hospital from September 2011 to September 2014 were divided into experimental group (36 cases) and control group (36 cases), according to different operation method, the experimental group using arthroscopic, while the control group using traditional incision surgery. Then the incision length, operative time, hospital stay, wound healing, bone healing time were compared. Complications, knee function and reduction of the fracture were compared. Results The incision length of experimental group was significantly lower than the control group, the difference was statistically significant (P <0.05). Operative time, hospital stay, wound healing time, the bone healing time were significantly lower than the control group, the differences were statistically significant (P < 0.05). Delay healing, joint stiffness and traumatic arthritis were significantly lower than the control group, the differences were statistically significant (P < 0.05). RASMUSSEN function score and radiographic scores in the experimental group were significantly higher than the control group, the differences were statistically significant (P < 0.05). Conclusion Fixation with knee arthroscopy as adjuvant therapy for lateral tibial plateau fracture has advantages of smaller incision, shorter operative time and hospital stay and healing time, lower complication rate while better reduction of the fracture and function than conventional methods.
2.INTRAUTERINE GROWTH RETARDATION AT FULL TERM PREGNANCIES WITH ENDOCRINE FACTORS
Ling WU ; Baoqin LIU ; Shuhua YU
Journal of Pharmaceutical Analysis 2000;12(1):73-76
Objective To investigate the relationship between intrauterine growth retardation (IUGR) and en docrine parameters so as to assess the effects of the main endocrine factors on IUGR. The concentrations of growth hormone (GH), insulin, T3, T4 and TSH were measured in umbilical cord blood, amniotic fluid and maternal serum. Methods The samples were collected from 23 pregnant women who were diagnosed as the full term IUGR, 42 normal full term pregnant women with normal infants' weight were taken as control. Growth hormone and insulin were mea sured by radioimmunoassay. T3, T4 and TSH were investigated by micro-radioimmunoassay. Results The concentra tions of growth hormone, insulin and T4 in umbilical cord blood were lower in IUGR than that in control group(GH 4. 63μg/L vs 7.01μg/L, insulin 10. 68μIU/ml vs 31.44μIU/ml, T4 87. 39nmol/L vs 138. 10nmol/L. P <0. 05, 0. 05 and 0. 05, respectively). The TSH concentration in umbilical cord blood was higher in IUGR than in control group (10. 84μmIU/L vs 5. 75μmIU/L, P <0. 01). The concentration of growth hormone in maternal serum and the concen tration of insulin in amniotic fluid were also lower in IUGR group than in control group(GH 1.77μg/L vs 2.74μg/L, P <0. 01, insulin 5. 84μIU/mi vs 15. 64μIU/ml, P <0. 01). Conclusion This study confirms that full term neonates with IUGR are abnormal in endocrine factors. The inadequacy of growth hormone may be one of the causes of IUGR. The relative scarcity of growth hormone and insulin seems to be a factor to compromise the fetus' metabolism. Be sides, the early hypothyrosis of infants with IUGR might protect them from unfavorable environment in the uterine.
3.THE SEGMENTAL AND REGIONAL PROJECTIONS OF THE SCIATIC,TIBIAL AND COMMON PERONEAL NERVES TO THE SUBSTANTIA GELATINOSA OF SPINAL CORD IN RATS
Shuhua QIU ; Shiji DAI ; Haixia WU
Acta Anatomica Sinica 1955;0(03):-
The segmental and regional projections of the sciatic, tibial and common peroneal nerves to the substantia gelatinosa of spinal cord in rats were studied with the acid phosphatase method. Experiments were carried on 42 rats. Under chloral hydrate anesthesia, sciatic, tibial and common peroneal nerves were severed respecively. Then, after a survival time of 5~12 days, the lumbosacral segments of the spinal cord were removed and sectioned. The frozen sections were incubated with the modified Gomori's method.The projections of the sciatic and tibial nerves to the substantia gelatinosa (SG)were found in L_2~S_1, while these of the common peroneal nerve in L_(2~6).The projections of the sciatic nerve were found in the most medial, the intermediate and the most lateral parts of SG in the segments L_(2~3), and in entire SG in the segments L_4~S_1.The projections of the tibial nerve were shown in the most medial and the intermediate parts of SG in the segments L_(2~5), and in the medial parts of SG at L_6~S_1.The projections of the common peroneal nerve were found in the intermediate parts of SG in all segments L_(2~6).The above study has provided anatomical data for probing into the mechanism of somatosplanchnic interrelations and the principle governing acupuncture anesthesia.
4.Experimental Study Progress on Tribendimidine,Artemether and Artesuante Against Clonorchis sinensis and other Trematodes
Shuhua XIAO ; Jian XUE ; Zhongxin WU
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(01):-
Currently praziquantel is one of the major drugs used in treatment of schistosomiasis and other trematode infections.Recent experimental studies indicate that a new anthelmintic,tribendimidine,is used in the treatment of intestinal nematodes,also possesses effect against several species of trematodes including Clonorchis sinensis,Opisthorchis viverrini and Echinostoma caproni.Tribendimidine is even more effective against C.sinensis in rats that a single 300 mg/kg oral dose cures almost all of the animals treated,a lower cure dose than praziquantel(375-500 mg/kg).The anti-malarial drugs artemether and artesunate are not only effective in the prevention of schistosomiasis,but also effective against several species of trematodes,especially C.sinensis.The single oral dose of both drugs to cure or achieve high efficacy in infected rats is 75 mg/kg.This review summarized research progress on tribendimidine,artesunate,and artemether in experimental animals infected with C.sinensis and other species of trematodes.
5.Transcutaneous Injection of MIIGX3 Artificial Bone and Methylprednisolone for Treatment of Recurrent Bone Cysts
Hongbin WU ; Jingyuan DU ; Shuhua YANG
Chinese Journal of Minimally Invasive Surgery 2005;0(08):-
Objective To investigate the efficacy of transcutaneous injection of MIIGX3 artificial bone and methylprednisolone for recurrent bone cysts.Methods From January 2004 to March 2006,a total of 13 children with recurrent bone cysts received transcutaneous injection of methylprednisolone and MIIGX3 in our hospital.X-ray was employed to detect the degradation of MIIGX3 and formation of new bones.Results The operation time ranged from 30 to 95 minutes(mean 42 minutes).The patients were followed up for 1 to 3 years.None of them had recurrence of bone cysts during this period.The artificial bones were completely degraded and replaced by new bones in 1.5 years after the injection.Conclusions Transcutaneous injection of methylprednisolone and MIIGX3 is effective for recurrent bone cysts.Patients have shorter operation time and hospital stay after this treatment because the procedure is microinvasive.
6.A retrospective analysis of 112 cases of adverse drug reaction of Chinese drug preparations
Jianhong WU ; Jianru SHI ; Huiyi CHEN ; Xingyi CHEN ; Shuhua CHEN
International Journal of Traditional Chinese Medicine 2012;34(2):109-112
Objective To study the characteristics and regularities of Chinese drugs preparations ADR in order to improve the rational drug use.Methods A total of 112 cases of TCM ADR reports collected in 2009~2011 which have been presented to the Guangzhou ADR Monitoring Center.Results Of the 112 cases,26 kinds of drugs were involved,quinquagenarian were the main crowd,intravenous infusion was the main route of administration,the main types of TCM ADR were lesions of skin and accessories.Conclusion Great importance should be attached to TCM ADR monitoring to promote rational drug use in the clinic.
7.THE AMPLIFICATION AND EXPRESSION OF c-erbB2 AND p53 IN ORAL SQUAMOUS CELL CARCINOMAS
Naiguo LIU ; Shuhua WU ; Changmin SHAN ; Zenghua LU ; Jie ZHANG ;
Acta Anatomica Sinica 1957;0(04):-
0 05); The protein expression of erbB2 in carcinomas of palate and floor of mouth were obviously higher than the expression in lip cancer and gingival carcinoma( P 0 05).Conclusion The amplification and overexpression of c erbB2 and p53 play important roles in the formation and development of OSCC, the overexpression of c erbB2 protein has more important significance in tumorigenesis of carcinomas of palate and floor of mouth; Not only transcriptional regulation but also translational modulation exists on the expression of c erbB2 and p53. [
8.A systematic review of hip fractures in elderly people
Wu ZHOU ; Guohui LIU ; Shuhua YANG ; Zengwu SHAO ; Bobin MI
Chinese Journal of Orthopaedics 2017;37(17):1093-1099
Objective To study the age peak of incidence,pathologic sites,therapeutic effect and complications of different treatment for hip fractures in the elderly.Methods Hip fractures,therapy,elderly were used as key words to search articles both in English and Chinese from 1979 to 2017.The articles were filtrated by title,abstract and full text and 32 of them were left.All the patients were objects of this study,but the repeated cases were excluded.The patients' data such as age of onset,gender,fracture type,operation method,follow-up time,and complications were collected and analyzed by systematic analysis.Results Data of 2 758 patients in 32 papers had been collected for analysis.There were 1791 femoral neck fractures and 967 intertrochanteric fractures,with a ratio of 1.85:1.The patients' age of onset was ranging from 54 to 92 years old,with an average of 76.7 years old,and the age peak of incidence was from 70 to 79 years old.The sex ratio was 1:1.66 (1 037 males:1721 females).All the patients had been followed up for 0.5 to 18 years,(average,7.8 years).Among 927 femoral neck fractures who were treated by 3 lag screws,there were 278 cases of non-union (29.9%),139 femoral head necrosis(15.1%) and 19 internal fixation loosening (2.0%);but for 183 femoral neck fractures who were treated by dynamic hip screws (DHS),there were 51 cases of non-union (28.1%),31 femoral head necrosis(17.0%),3 internal fixation losening(1.8%),and 27 cutting effect (15%).Then for 400 femoral neck fractures who were treated by hemiarthroplasty,there were 6 cases of dislocation(1.5%),14 shaft fracture (3.5%),8 deep infection (2.0%);while for 281 femoral neck fractures who were treated by total hip arthroplasty,there were 14 cases of dislocation (5.0%),11 shaft fracture (4.0%),and 10 deep infection (3.5%).On the other hand,for 354 intertrochanteric fractures who were treated by proximal femoral nail or proximal femoral nail antirotation internal fixation (PFN or PFNA),there were 18 cases of trochanter fracture (5.0%),28 hip varus (8.1%),32 displacement (9.0%);and for 210 intertrochanteric fractures who were treated by Gamma nail,there were 17 cases of trochanter fracture (7.9%),11 cutting effect (5.0%),6 displacement (3.0%);and for 135 intertrochanteric fractures who were treated by DHS,there were 8 cases of cutting effect (6.1%),7 displacement (5.2%);and for 101 intertrochanteric who were treated by proximal femoral locking plate,there were 5 cases of displacement (5.0%);Also for 89 intertrochanteric fractures who were treated by hemiarthroplasty,there were 2 cases of dislocation(1.8%),3 shaft fracture (3.0%),1 deep infection(1.5%);and for 78 intertrochanteric fractures who were treated by total hip arthroplasty,there were 4 cases of dislocation (4.8%),3 shaft fracture (4.0%),2 deep infection (2.5%).Conclusion The elderly hip fractures' age peak of incidence is from 70 to 79 years old,and femoral neck fracture takes up a majority of it.Female patients are more than male patients.The femoral neck fractures are mainly treated by 3 lag screws internal fixation;non-union and femoral head necrosis are the common complications postoperation.While the intertrochanteric fractures are mainly treated by proximal femoral nails;displacement,hip varus and trochanter fractures are the common complications postoperation.The artificial hip replacement is the effective remedy measure for secondary femoral head necrosis after hip fractures.
9.Effects of HDAC5 on the proliferation and apoptosis of the gastric cancer cell line SGC-7901
Yong ZHANG ; Chen YANG ; Xinzhi WANG ; Yubao XIONG ; Shuhua WU
Chinese Journal of Clinical Oncology 2017;44(10):469-473
Objective: To investigate HDAC5 expression in gastric cancer cell lines and its effects on the proliferation and apoptosis of the gastric cancer line SGC-7901. Methods: The expression patterns of HDAC5 and Twist1 in gastric cancer cell lines and normal gastric mucosal cells were detected by Western blot. The effects of HDAC5 and Twist1 on the proliferation and apoptosis of SGC-7901 cells were analyzed by MTT and flow cytometry, respectively. Results: The expression of HDAC5 and Twist1 in gastric cancer cell lines were significantly higher than that in normal gastric mucosal cells (P<0.05). HDAC5 knockdown significantly down-regulated Twist1 expression,inhibited cell proliferation, and induced apoptosis in SGC-7901 cells, whereas HDAC5 overexpression exhibited an opposite effect (P<0.05). Moreover, Twist1 knockdown significantly inhibited cell proliferation and induced apoptosis in SGC-7901 cells (P<0.05). Conclusion:HDAC5 may promote cell proliferation and inhibit apoptosis in gastric cancer cells by upregulating Twist1 expression, thus promoting the initiation and development of gastric cancer.
10.Establishment and evaluation of rat model of acute stress during chronic heart failure
Xiaoxia ZHANG ; Xuesi WU ; Zhihong HAN ; Shuhua MI
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
Objective To establish the rat model of acute stress during chronic heart failure and evaluate it.Methods Male Wistar rats were injected adrimycin(ADR)via abdominal cavity to induce heart failure.Cardiovascular dynamics and heart pathology were measured.Rats with heart failure received bacterial lipopolysaccharide(LPS)0.2 mg/kg by peritoneal injection to induce acute stress,and we measured blood pressure,heart rate and the expression of HO-1 after LPS injection.Results ADR peritoneal injection led to heart failure successfully.Compared with that in normal group,left ventricular function(?LVdp/dtmax)of rats in model group reduced significantly,and myocardial fibrosis and hypertrophy occurred,too.LPS peritoneal injection led to acute stress in rats with heart failure.Heart rate and blood pressure began to rise 0.5 h after LPS injection and reached at 1 h after LPS injection.The expression of myocardium HO-1 increased at 6 h and was significantly higher at 12 h after LPS injection.The expression of HO-1 was higher in model group than in normal group.Conclusion LPS peritoneal injection successfully leads to acute stress in rats with heart failure,providing a reliable model for study of decompensated heart failure.